Development and evaluation of enzyme-linked immunosorbent assay based on recombinant inorganic pyrophosphatase gene antigen for the detection of Mycoplasma suis antibodies

The recombinant ppa protein of Mycoplasma suis migrated to 21 kDa. Using this antigen, an ELISA system to detect the antibody against M. suis infection in swine was established. The rELISA demonstrated 98.5% specificities among negative samples and 96.9% sensitivity among positive samples with M. suis infection. A comparison of this ELISA system with an indirect hemagglutination assay (IHA) test using 132 swine samples revealed that the positive rate was 34.0% in ELISA and 28.0% in IHA. Compared with IHA, the present rELISA system using recombinant ppa antigen significantly improves the specificity, sensitivity, and stability for serodiagnosis of M. suis infection in swine.     

利用轻小型飞机遥感数据建立人工林特征参数模型

目前获取森林特征参数的主要方法是外业测量,工作量大、效率低。该文以中国自主研发的轻小型航空遥感系统为数据获取工具,以油松人工林为研究对象,通过对获取森林的激光雷达（light detection and ranging,LIDAR）点云数据去噪,分类,提取等过程获得单木的树高数据,对获取的航空影像数据进行预处理,匹配,拼接,分割及冠幅提取获得单木的冠幅数据,再与外业抽样调查的单木的树高、胸径建立回归模型,同时验证模型精度。试验结果表明：通过LIDAR点云数据提取的树高与实测的树高具有极显著的相关性,所建立的模型预测精度达97.5%,通过影像提取的冠幅与实测的胸径也具有极显著的相关性,预测精度达91.6%,基本上能够满足林业生产的要求。     

基于NPP/VIIRS夜光遥感影像的作业灯光围网渔船识别

为对远洋灯光渔船作业信息进行实时动态监测,该研究基于可见光红外辐射仪(visible infrared imaging radiometer suite,VIIRS)夜光遥感影像,根据远洋灯光渔船作业时其集鱼灯灯光在VIIRS白天/夜晚波段(day/night band,DNB)影像上的辐射特征,采用峰值中值指数(spike median index,SMI)对灯光渔船与背景像元间的辐射差异进行拉伸,在此基础上设计了基于最大熵法(maximum entropy method,Max Ent)阈值分割以及局部峰值检测(local spike detection,LSD)的作业远洋灯光渔船识别算法,并采用2015年西北太平洋公海灯光围网渔场内作业渔船船位监控系统(vessel monitoring system,VMS)数据对该算法的识别精度进行检验。验证结果显示,该文提出的作业远洋灯光渔船自动识别算法对实际作业灯光渔船的识别精度在92%以上,可以满足远洋灯光渔船日常监测的需求,可为进一步评估远洋光诱渔业捕捞努力量、推进远洋光诱渔业信息化管理以及打击非法、未申报和无管制的(illegal,unregulated,unreported,IUU)捕捞活动提供技术支持。     

覆膜滴灌棉田蒸散量的模拟研究

通过综合考虑影响作物蒸散量的土壤、作物、大气3方面因子,结合新疆滴灌棉田覆膜栽培的生产实际,设计了不同覆盖度和品种试验,以Penman－Montieth方程估算参考作物蒸散量,确定了不同覆盖度及品种条件下的作物系数,并在此基础上实现了覆膜滴灌棉田蒸散量较为准确地估计。试验结果认为覆膜滴灌棉田全生育期蒸散量在540～620 mm之间,全生育期蒸散量和作物系数都随着覆盖度的增加而减小,叶面积指数与日蒸散量及作物系数关系密切,品种间由于品种特性的差异而引起的叶面积指数变化,最终导致了品种间作物系数K_c的不同。     

Effect of drought pretreatment before anthesis and post-anthesis waterlogging on water relation,photosynthesis, and growth of tomatoes

The effect of drought preconditioning before anthesis and post-anthesis waterlogging on water relation, photosynthesis, and growth was studied in tomatoes. Tomatoes were grown in pots and exposed to four treatments, whereby the plants were irrigated to 80% field capacity in T1 (control) and T2, 70% of the control (T3), and 50% of the control (T4). Drought was maintained for 30 days from 14 days after transplanting (DAT), and then the plants under T2, T3, and T4 were subjected to waterlogging at 60 and 80 DAT and lasted for 2 days. The results showed that drought pretreatments induced a decrease in leaf water potential, leaf insertion angle, photosynthetic rate, and transpiration rate. The stomatal closure and epinasty observed in response to drought pretreatment represented adaptive mechanisms to the followed waterlogging. The soil redox potential, photosynthetic rate, stomatal conductance, and transpiration rate of un-pretreatment were dramatically decreased by post-anthesis waterlogging; however, T3 was found to effectively enhance tolerance to a waterlogging event by decreasing leaf insertion angle and increasing photosynthetic rate, stomatal conductance, and transpiration rate. Fruit quality and yield were deteriorated considerably by waterlogging. However, T3 caused less damage to fruit quality and yield by post-anthesis waterlogging compared to T2.     

不同地下水位下渠基冻胀规律与保温板适宜厚度确定

为探明河套灌区不同地下水位条件下渠基冻胀的差异,了解地下水位变化对渠基土壤水分迁移和力学性能的影响,该文通过建立不同地下水位冻胀试验平台并结合原型渠道,分析了不同地下水位对铺设不同厚度聚苯板的基土冻胀的影响,阐述了冻胀率和截面弯矩沿渠坡的分布规律,提出了不同地下水位下聚苯板适宜铺设厚度的理论计算公式。试验得出,每降低1 cm地下水位,基土冻胀量减小0.15 cm。当地下水位降低0.5~1.0 m后,削减冻胀率为71%~83.8%。地下水位下降有效阻止或延缓了土壤毛细水的上升,降低了0~30 cm土层的土壤水分,有效阻止了冻结锋面水分的迁移,减小了土体中冰夹层的形成,从而降低了土体的冻胀变形。并且,渠道的冻胀破坏部位随着地下水位的不同而发生变化。该研究可为北方季节性冻土区骨干渠道保温防冻胀技术研究提供科学依据和技术支撑。     

Changes in the pH,EC, available P,SOC and TN stocks in a single rice paddy after long-term application of inorganic fertilizers and organic matters in a cold temperate region of Japan

Purpose

The objective of this study was to determine the changes in the main soil chemical properties including pH, electrical conductivity (EC), available phosphorus (P), soil organic carbon (SOC) and total nitrogen (TN) stocks after long-term (31 years) additions of two types of organic matters—rice straw and rice straw compost, combined with NPK fertilizers in single rice paddy in a cold temperate region of Japan.

Materials and methods

A long-term experiment on combined inorganic fertilizers and organic matters in paddy rice cultivation began in May 1982 in Yamagata, northeastern Japan. After the 31st harvest, soil samples were collected from five treatments [(1) PK, (2) NPK, (3) NPK + 6 Mg ha^?1 rice straw (RS), (4) NPK + 10 Mg ha^?1 rice straw compost (CM1), and (5) NPK + 30 Mg ha^?1 rice straw compost (CM3)] at five soil depths (0–5, 5–10, 10–15, 15–20, and 20–25 cm). Soil chemical properties of pH, EC, available P, SOC, and TN were analyzed.

Results and discussion

The pH decreased significantly only at the higher compost rate of 30 Mg ha^?1, while EC increased in all the organic matter treatments. Available P significantly increased in the CM1 and CM3 treatments by 55.1 and 86.4 %. The amounts of SOC stock increased by 67.2, 21.4, and 8.6 %, and soil TN stock by 64.1, 20.2, and 8.5 % in CM3, RS, and CM1, respectively, compared to NPK treatment.

Conclusions

Significant changes in soil properties were observed after 31 years of organic matter applications with reference to PK- and NPK-fertilized rice paddy soils. A significant decrease in pH was observed with the application of a high rate (30 Mg ha^?1) of rice straw compost but not with the conventional rate of 10 Mg ha^?1. However, EC increased significantly relative to that of the PK- and NPK-fertilized plots in all the organic matter treatments. Available P significantly increased in the CM1 and CM3 treatments by 55.1 and 86.4 %. The amounts of SOC stock expressed as a percentage of total C applied to the soil were higher from 10 Mg ha^?1 compost (28.7 %) than that from 6 Mg ha^?1 rice straw (17.4 %), indicating a more effective soil organic C accumulation from rice straw compost than that from original rice straw.

     

禽传染性喉气管炎病毒体内外感染模型中鸡Toll样受体21mRNA转录水平的研究

为研究病毒与机体的相互作用,本研究参考GenBank中鸡Toll样受体21 (ChTLR21)的基因序列设计实时定量PCR特异性引物,以鸡核糖体蛋白L4 (RPL4)为内参基因,建立检测ChTLR21 mRNA相对转录水平的实时定量PCR方法,分析ChTLR21在禽传染性喉气管炎病毒(ILTV)感染的鸡胚成纤维细胞(CEF)中和感染SPF雏鸡免疫器官脾脏、法氏囊和胸腺组织中的转录水平.结果显示:ILTV感染CEF后2h、4h、8h和18h时间点ChTLR21 mRNA转录水平分别为未感染对照细胞的1 540.53、0.98、1.19和3.70倍.但仅在ILTV感染2h时引起ChTLR21转录水平升高,与对照组相比差异显著(P＜0.05).ILTV感染SPF雏鸡后6h、24 h和30 h脾脏中ChTLR21 mRNA转录水平分别为未感染对照的56.34、59.85和3.61倍;法氏囊中分别为0.03、25.98和3.08倍;胸腺中分别为2.52、50和7.32倍.在感染初期,脾脏中ChTLR21转录量显著升高,随后有所降低,但均高于未感染对照(p＜0.05);法氏囊中仅在感染6h时呈显著抑制(p＜0.01);胸腺中呈波动性转录水平升高,但与对照组无统计学差异(p＞0.05).本研究证明ChTLR21参与了鸡体对ILTV感染的应答,并在体内外感染模型中呈现不同的表达规律.     

玛曲高寒草甸放牧强度的遥感监测

为给玛曲县高寒草甸草-畜平衡和草地资源的可持续利用提供科学依据,本研究利用遥感技术,结合2016年地面实测数据和同时期的MODIS影像资料,分别建立了研究区草地地上生物量和植被指数(NDVI)、相对放牧强度间的回归模型以及不同相对放牧强度和NDVI值之间的幂回归模型。结果表明,6-9月调查点NDVI和地上生物量间存在较好的相关性,即随着NDVI值的增加生物量呈上升趋势,且9月份的拟合(R2=0.510 2)达到了极显著水平;实测地上生物量和相对放牧强度之间有很好的拟合关系(R2=0.965 1),随着放牧强度的增加地上生物量呈下降趋势;相对放牧强度和植被指数间相关性较好(R2=0.631),即随着NDVI值的变小,相对放牧强度逐渐增强,当相对放牧强度增强到一定程度时,NDVI对其响应不灵敏。     

用低场核磁共振检测水稻浸种过程中种子水分的相态及分布特征

为研究水稻浸种过程中种子的水分相态及其分布特征,利用低场核磁共振快速、无损、准确的检测技术,通过硬脉冲回波序列CPMG(carr-purcell-meiboom-gill sequence)测量水稻种子横向弛豫时间T2,根据横向弛豫时间T2的差异区分种子内部的水分相态及其变化规律。试验结果表明:通过T2反演谱横向弛豫时间T2长短的差异,发现水稻浸种过程中种子内部水分存在结合水、自由水2种水分状态,同时可区分出内层水、中层水、外层水3种水分分层;二者均能通过回归方程合理的估测水稻在浸种过程中种子的吸水率情况;通过T2反演谱信号幅值大小的差异,发现水稻浸种过程中的种子总水含量不断上升,但由于判定依据及划分方式的不同,二者在水分的流动方式上略显差异。低场核磁共振技术对水稻浸种过程中种子内部的水分变化进行了直观的揭示,提供了一种高效的种子水分检测方法。