大熊猫铜绿色假单胞菌感染诊断

北京动物园1只大熊猫腹泻,粪便呈浅红色、带粘液和胶冻样.经对粪便作细菌培养,分离出铜绿色假单胞菌(Pseudomonas aeruginosa).该菌对多种抗生素不敏感,对小白鼠有较强的致病力,是引起本次大熊猫腹泻的病原菌.介绍如下.     

猪细小病毒病的诊断

１发病情况河北省某集约化猪场饲养繁殖母猪５００余头,存栏猪５０００余头,自１９９５年建场以来一直未出现过重大疫情。１９９８年１０月份,收稿日期：１９９８－１１－１３该场从１０月１５日至１０月２５日仅１０天时间,产仔数为２２５头,其中木乃伊胎３２头,死...     

鸡布氏艾美耳球虫致病性研究

用１８日龄公雏作为试验对象,以增重、病变和死亡为判定指标,用Ｅ．ｂｒｕｎｅｔｉ的０．５万／只、１万、５万、１０万、２０万、５０万和１００万／只为接种梯度,研究了布氏艾美耳球虫的致病性。结果表明：接种０．５万／只至２０万／只剂量的Ｅ．ｂｒｕｎｅｔｉ都明显影响鸡的增重;（与对照组相比,均存在极显著性差异（Ｐ＜０．０１）,各剂量组间增重无差异,）５０万／只和１００万／只剂量组的鸡出现减重。５０万／只剂量组鸡死亡５０％,１００万／只剂量组鸡死亡１００％;Ｅ．ｂｒｕｎｅｔｉ接种５万／只剂量组及以上各组鸡饮食欲消失,鸡失去自洁行为,粪量少,含有粘液和血液;剖检病变主要在小肠至直肠,肠壁变薄,易碎,呈粉红色至暗红色,肠粘膜上有小的出血点,肠内容物以粘液和血液为主。部分鸡的盲肠内容物干、呈暗红色。（病变计分为：对照组０分,０．５万／只为０．５７＋;１万／只０．０３＋;５万／只１．１７＋;１０万／只１．５０＋;２０万／只２．２３＋;５０万／只３．６４＋;１００万／只３．８３＋。）１００万／只剂量组中有两只鸡出现腿麻痹症状,不能站立。     

鸡传染性喉气管炎病毒姻台株gB基因的序列测定及其结构功能分析

研究以鸡传染性喉气管炎病毒（ILTV）烟台株为模板，PCIK扩增出1条约2．7的gB基因片段，并将其克隆到pMD18-T载体中。序列测定显示，gB基因长2622bp，包含完的阅读框架。序列比较分析发现，烟台株和王岗株、河北株的班基因核苷酸序列与SA2株的比均在第89位上缺失1个碱基G，而在第102位核苷酸处插入1个碱基A，从而引起氨基酸序中第29-32位5个氨基酸的移码突变。烟台株还有另外7个碱基发生突变，使得其班基因与北株、王岗株、SA2疫苗株的gB基因核苷酸同源性分别为99．8％、99．7％和99．6％，氨基酸的同性分别为99．4％、99．4％、98．9％，表明不同ILTV毒株之间gB基因是非常保守的。划型相列河源     

新城疫活疫苗对控制鸡大肠杆菌病的效果观察

为了研究鸡新城疫活疫苗紧急免疫控制鸡大肠杆菌病的效果,通过大肠杆菌人工感染30日龄蛋鸡,待出现病症后,紧急免疫新城疫La-sota弱毒苗(Ⅳ系)4羽份.免疫后5～17 d,通过血凝抑制试验测定血清新城疫抗体水平,试验组新城疫抗体水平逐步提高,对照组抗体略有下降,免疫后17 d两组抗体水平差异显著(P＜0.05);对照组...     

微机地理信息系统软件PC ARC／INFO功能简介

本文主要介绍了微机地理信息系统软件 PC ARC/INFO 的结构,主模块 ARC 及其主要子模块的功能,系统优化的方法及该系统当前的主要应用领域及前景展望。     

绵羊肺炎支原体黏附因子的定点突变及原核表达

本研究采用点突变PCR方法成功克隆并表达了绵羊肺炎支原体(Mycoplasma ovipneumoiae,M.O)内蒙古分离株(SZWQ株)的黏附因子基因(SZWQ-860)。表达产物经SDS-PAGE电泳检测,与预期目的蛋白的大小一致,将重组蛋白纯化后经Western blot检测,结果表明重组蛋白具有良好的免疫反应性。     

养猪生产的收益和成本调查

自2007年5月生猪价格上涨以来,猪价一直高位运行,养猪生产效益可观。为及时了解养猪生产中各个环节的收益和成本情况,特进行了专题调查,现报告如下。1养猪收益根据笔者对养猪生产中的饲料、饲养、屠宰、销售等环节的调查,以一头猪为例,收益情况大体如下。1.1饲料生产环节可获利     

The 16MΔvjbR as an ideal live attenuated vaccine candidate for differentiation between Brucella vaccination and infection

Brucellosis brings great economic burdens for developing countries. Live attenuated vaccines are the most efficient means for prevention and control of animal Brucellosis. However, the difficulties of differentiating of infection from vaccine immunization, which is essential for eradication programs, limit their applications. Therefore, the development of a vaccine that could differentiate infection from immunization will overcome the limitations and get extensive application. VjbR is a quorum sensing regulator involving in Brucella's intracellular survival. The vjbR∷Tn5 mutants have been proven effective against wild type strain challenge, implying its possibility of use in vaccine candidate development. To further evaluate this candidate gene, in the present study, the antigenicity of purified recombinant VjbR protein was analyzed. Antibodies to Brucella melitensis VjbR could be detected in sera from patients and animals with brucellosis but not in control ones, implying the potential use of this protein as a diagnostic antigen. Then a vjbR mutant of B. melitensis 16M was constructed by replacing the vjbR with kanamycin gene. The mutant showed reduced survival in macrophage and mice. Vaccination of BALB/c mice with 16MΔvjbR conferred significant protective immunity against B. melitensis strain 16M challenges, being equivalent to which induced by the license vaccine Rev.1. The vjbR deletion mutant elicited an anti-Brucella-specific immunoglobulin G response and induced the secretion of gamma interferon and interleukin-10. The most importance is that, the use of vjbR mutants as vaccines in association with diagnostic tests based on the VjbR antigen would allow the serological differentiation between infected and vaccinated animals. These results suggest that 16MΔvjbR is an ideal live attenuated vaccine candidate against B. melitensis and deserves further evaluation for vaccine development.     

Establishment of Detection Methods for Dexamethasone Sodium Phosphate in Injections by UPLC-PDA

A detection method of dexamethasone sodium phosphate in houttuynia injection,bupleurum injection,vitamin C injection,lincomycin hydrochloride injection,and asragulus polysacharin injection was established by UPLC-PDA.The Waters ACQUITY UPLC^® BEH C18 column(2.1 mm×50 mm,1.7 μm) was used,the mobile phase consisted of 0.75% triethylamine phosphatic liquor/solution (pH 3.0)-methyl alcohol-acetonitrile(50:45:5),the flow rate was 0.3 mL/min,the column temperature had remained 25℃,photodiode array detector wavelength range was 190 to 400 nm,the recorded wavelength was 242 nm,the injection volume was 10 μL.The linear range of dexamethasone sodium phosphatel was 10.0 to 200.0 mg/L (R²=0.9999),the mean recovery and RSD for dexamethasone sodium phosphate in houttuynia injection,bupleurum injection,vitamin C injection,lincomycin hydrochloride injection and asragulus polysacharin injection were 97.54% and 1.14%,101.59% and 0.19%,100.92% and 0.92%,99.82% and 0.73%,100.08% and 0.62%,respectively.This method was accurate,easy and fast,it was suitable for detection of dexamethasone sodium phosphatein houttuynia injection and other four kinds of injections.