Oncolytic Vaccinia Virus Carrying Aphrocallistes vastus Lectin (oncoVV-AVL) Enhances Inflammatory Response in Hepatocellular Carcinoma Cells

Aphrocallistes vastus lectin (AVL) is a C-type marine lectin derived from sponges. Our previous study demonstrated that oncolytic vaccinia virus carrying AVL (oncoVV-AVL) significantly enhanced the cytotoxicity of oncoVV in cervical cancer, colorectal cancer and hepatocellular carcinoma through the activation of Ras/ERK, MAPK/ERK and PI3K/Akt signaling pathways. In this study, the inflammatory response induced by oncoVV-AVL in a hepatocellular carcinoma cell (HCC) model was investigated. The results showed that oncoVV-AVL increased the levels of inflammatory cytokines including IL-6, IL-8 and TNF-α through activating the AP-1 signaling pathway in HCC. This study provides novel insights into the utilization of lectin AVL in the field of cancer therapy.     

池塘网箱配合饲料养殖鳜鱼技术研究

为研究池塘网箱配合饲料养殖鳜鱼技术,在3个池塘中进行人工生态设置,架设不超过池塘总面积10%的网箱,网箱外投放不同生态位与食性的鱼类控制池塘生物总量,网箱中投放鳜鱼(Siniperca chuatsi Basilewsky)苗种,全程投喂配合饲料,结合投喂以中草药成分制作的药饵。试验结果表明,养殖期间未发生病害,3个池塘水质52.4%的时间为轻度富营养化,47.6%的时间为中度富营养化;商品鳜鱼产量15～18 kg/m2,折算池塘平均产量12 700～14 400 kg/hm2,养殖利润194 400～249 900元/hm2,商品鳜鱼规格整齐,起捕销售便捷,经济效益和生态效益显著。     

哈萨克母羊分娩前与胎儿组织器官细菌的多样性

[目的]研究分娩前哈萨克母羊和胎儿组织、肠道内容物及相关器官菌群的多样性.[方法]试验选择5只4岁、胎次相同、健康的妊娠后期母羊,平均体重为(55.64±4.37)kg,安乐死后,分离各组织器官并采集相关样品,用于分析菌群多样性.[结果](1)母羊唾液、小肠内容物及羊水中有较高的多样性,chao1指数分别为1 042....     

黄淮海地区不同种植结构下点蜂缘蝽的发生规律

为明确大豆-玉米带状间作种植模式对点蜂缘蝽Riptortus pedestris发生规律的影响,2023年在山东省大豆单作田中进行诱捕效果试验,筛选诱捕效果最好的诱捕器对黄淮海地区山东、河南和安徽3个省6个市不同种植模式下点蜂缘蝽的发生动态进行监测。结果显示,风叶型诱捕器对点蜂缘蝽的诱捕效果最佳。在大豆单作田内点蜂缘蝽的发生高峰期为7月中旬—8月中旬,此时大豆正值盛花-始荚期。在大豆-玉米带状间作田内点蜂缘蝽的发生动态与大豆单作田的无明显差异,但点蜂缘蝽发生数量及高峰期发生时间可能与杀虫剂的施用时间和次数密切相关。     

草地贪夜蛾核型多角体病毒对草地贪夜蛾的毒力及其侵染特征

为明确草地贪夜蛾核型多角体病毒（Spodoptera frugiperda multiple nucleopolyhedrovirus,SfMNPV）对草地贪夜蛾Spodoptera frugiperda 的毒力及其体内外侵染特性,于室内测定SfMNPV对草地贪夜蛾的毒力,采用苏木精-伊红（hematoxylin-eosin,HE）染色分析SfMNPV侵染后草地贪夜蛾的组织病理学特征,通过实时荧光定量PCR（real-time fluorescence quantitative PCR,qPCR）技术分析SfMNPV在草地贪夜蛾体内和Sf9细胞系中的复制特征。结果表明,SfMNPV对草地贪夜蛾2龄幼虫的LC₅₀和LT₅₀分别为7.39×10⁵ OBs/mL和3.82 d;SfMNPV可侵染草地贪夜蛾幼虫的真皮、气管、脂肪体和中肠等组织,未侵染肌肉组织;SfMNPV侵染96 h后草地贪夜蛾体内SfMNPV基因组拷贝数最大,为3.91×10⁶ 拷贝数/ng,SfMNPV侵染96 h后Sf9细胞培养上清液中SfMNPV基因组拷贝数最大,为2.08×10⁷ 拷贝数/mL。     

浅谈克服东魁杨梅大小年的关键技术

近几年来,我镇果农大量种植杨梅,经统计全镇共种植320.6亩.由于品种较杂,存在产量低、品质差,平均每亩可增产223.9kg左右,个别年份虽产量高但品质差,增产不增收的现状.     

平菇菌盖超微结构的研究

利用透射电镜，对平菇菌盖的超微结构进行了观察。平菇成熟菌丝细胞有完整的双层细胞壁结构，内含细胞膜，线粒体，细胞核，内质网，泡囊，核糖体，糖原、须边体及典型的桶孔隔膜，有的细胞中，糖原颗粒了聚集在细胞质中，泡囊中也含有大量颗粒。线粒体膜，核膜，孢子膜均为双层，轮廓清晰。孢子中有线粒体、核、泡囊、核糖体、内质网。老化菌丝内有较大液泡，不见各种细胞器。     

RAPD标记鉴定果树芽变的可行性分析

在综合分析果树芽变的遗传特点以及RAPD标记技术的原理与存在问题的基础上，结合已有的研究报道，对RAPD标记应用于果树芽变鉴定中的可行性进行了探讨。对于不同的果树芽变类型，RAPD标记技术表现出不同的鉴定可行性。同时，对某些有关的问题提出了相应的建议。     

不同成熟度黄皮果实品质及贮藏特性的差异

黄皮Clausena lansium(Lour.)Skeels是我国南方原产的热带亚热带常绿果树。黄皮果实酸甜可口,风味独特,营养价值高且具有保健及药用价值,是深受人们喜爱的特产果品。近年来,我国黄皮栽培面积不断扩大,产量逐年提高。但黄皮采后极不耐贮藏,在自然条件下极易变软腐烂,1天后即明显     

Identification,Characteristics and Function of Phosphoglucomutase (PGM) in the Agar Biosynthesis and Carbon Flux in the Agarophyte Gracilariopsis lemaneiformis (Rhodophyta)

Agar is widely applied across the food, pharmaceutical and biotechnology industries, owing to its various bioactive functions. To better understand the agar biosynthesis in commercial seaweed Gracilariopsis lemaneiformis, the activities of four enzymes participating in the agar biosynthesis were detected, and phosphoglucomutase (PGM) was confirmed as highly correlated with agar accumulation. Three genes of PGM (GlPGM1, GlPGM2 and GlPGM3) were identified from the G. lemaneiformis genome. The subcellular localization analysis validated that GlPGM1 was located in the chloroplast and GlPGM3 was not significantly distributed in the organelles. Both the GlPGM1 and GlPGM3 protein levels showed a remarkable consistency with the agar variations, and GlPGM3 may participate in the carbon flux between (iso)floridoside, floridean starch and agar synthesis. After treatment with the PGM inhibitor, the agar and floridean starch contents and the activities of floridean starch synthase were significantly decreased; products identified in the Calvin cycle, the pentose phosphate pathway, the Embden-Meyerhof-Parnas pathway and the tricarboxylic acid cycle were depressed; however, lipids, phenolic acids and the intermediate metabolites, fructose-1,6-phosphate were upregulated. These findings reveal the essential role of PGM in regulating the carbon flux between agar and other carbohydrates in G. lemaneiformis, providing a guide for the artificial regulation of agar accumulation.