不同投喂方式对革胡子鲶消化酶的影响

在（25±1）℃水温、自然光照条件下,设3种投喂方式饲养革胡子鲶（clarias leath-er）：第1种连续投喂整个试验期间不间断,第2和3种分别连续投喂3、7 d 后饥饿1 d,3种方式分别以R0（对照组）、R1/3、R1/7表示,试验周期共45 d。结果显示：R0、R1/3和R1/7组胃、后肠和肝脏中蛋白酶活性影响差异不显著,但前肠和中肠内蛋白酶活性影响存在差异(P<0.05)。R0、R1/3、R1/7组的胃、前肠、中肠、后肠和肝脏中淀粉酶影响不显著,R0、R1/3、R1/7的胃、前肠和肝脏内脂肪酶活性影响差异显著(P<0.05),而R0、R1/3、R1/7组中肠和后肠中脂肪酶活性影响差异不显著(P>0.05)。     

急性感染肝片吸虫水牛的某些免疫反应研究

本实验选用12头体重为300－500kg，年龄2－3岁的雄性去势水牛，经粪便检查和Dot-ELISA检测确认为无肝片吸虫感染后，随机分为感染组（n=9）和对照组（n=3）。感染组每头水牛1次性经口感染1600个肝片吸虫囊蚴。实验期间由专人饲养，自由采食青干草和饮水。每周定时颈静脉采血，测定外周血淋巴细胞增殖反应、白细胞介素－2（IL－2）水平、T和B淋巴细胞比例、IgG水平以及血清蛋白总量和比例。结果表明：感染组水牛的T淋巴细胞刺激指数在感染后2－8周高于对照组，但刺激指数和IL－2与对照组均无显著性差异。感染组水牛的外周血T淋巴细胞比例从感染后第1周显著下降，以后一直在低于对照组水平波动，而淋巴细胞从感染后第1周开始显著升高，且一直在高于对照组范围内波动。感染组水牛的α-球蛋白、β－球蛋白与对照组相比均无显著性差异，而白蛋白从第5周开始显著下降，以后维持在低于对照组的水平范围波动；γ－球蛋白从第5周显著高于对照组，以后于对照组范围波动。感染组水牛的抗ES抗原（分泌－排泄抗原）的IgG抗体水平从第2周升高，并持续至实验结束。结果提示：体液免疫应答反应是水牛抗肝片吸虫感染的重要机制。     

The expression of genes related to adipocyte differentiation in pigs

The purpose of this study was to detect differential expression of genes related to adipocyte differentiation in pigs by suppression subtractive hybridization. Adipocytes and stromal vascular cells (a fraction containing preadipocytes) from pig adipose tissue were isolated for mRNA extraction. The cDNA from preadipocytes was subtracted from the cDNA from adipocytes. The subtracted gene fragments were cloned into pGEM-T Easy TA cloning vector. We selected 384 clones for gene sequence determination and for further analysis. These genes were subjected to a differential screening procedure to confirm the differential expression of genes between the 2 cell types. We found that at least 36 genes were highly expressed in the adipocytes compared with preadipocytes. Among these, 6 genes including 2 novel genes with the greatest differences were selected and confirmed by Northern analysis. We found that angiotensin I-converting enzyme (ACE), ataxia-telangiectasia mutated protein (ATM), calpain 1, and stearoyl coenzyme A desaturase 1 (SCD1) were highly expressed in adipocytes compared with preadipocytes (P < 0.05). The relative mRNA abundance of ACE, ATM, calpain 1, SCD1, and 2 novel genes discovered in the current study was increased at the later stages of adipocyte differentiation (P < 0.05). The results confirmed that the genes involved in lipid metabolism and adipocyte differentiation were highly expressed in porcine adipocytes. However, further investigation is needed to demonstrate specific functions of the novel genes discovered in the current study.     

关于生物安全隔离区建设试点的几点思考

动物疫病区划管理是当前国际通行的动物疫病管理措施,目的是实现动物疫病控制或净化,提高动物卫生水平,促进动物、动物产品的国际贸易。2003年,世界动物卫生组织(OIE)提出了动物疫病区域化管理新模式--生物安全隔离区划,即在同一生物安全管理系统下,通过采取监测、控制和生物安全管理措施,建立并维持无一种或多种特定动物疫病状态的无疫生物安全隔离区。虽然该概念提出时间较短,但已引起了全世界范围的广泛关注,欧盟和澳大利亚、泰国等一些畜牧业发达国家都在进行积极地研究和探索。我国也在无疫示范区建设基础上,根据OIE有关标准和建议,研究制定生物安全隔离区建设和评价标准,并着手开展试点工作。     

胸膜莫肺炎放线杆菌APXIV部分基因片段的表达及纯化

参照GenBank中已发表的ApxIV基因序列,以自行分离的App DNA为模板,利用PCR方法扩增出ApxIV3'端,大小为552bp的保守基因序列.将PCR产物克隆到pMD18-T Simple Vector中,获得重组质粒pMD-ApxIV,对其重组质粒pMD-ApxIV进行BamHI、HindIII双酶切,并将酶切产物克隆到原核表达载体pET-32a(+)中,构建了重组表达质粒pET-ApxIV.将表达质粒转化至大肠杆菌BL21中,用IPTG诱导表达,通过SDS-PAGE和Western blot分析,结果表明pET-ApxIV在BL21中成功表达,并能被App阳性血清所识别,具有良好的免疫原性.表达蛋白的分子质量约为39.5KDa.利用HiTrap FF crude columns将表达的蛋白进行了纯化.     

辉煌后涅槃 涅槃中轮回——2009豆市年度投资报告

经过2008年全球金融危机引爆的资本市场“去杠杆化”后．全球大豆市场走势将逐步回归供需面的引导。由于全球走出经济危机尚需时日,2009年国内外大豆期价很难走出单边行情．预期将更倾向于宽幅区间振荡行情。如果2009年美豆种植面积减少．2009年下半年经济好转,流动性再度充裕．那么2009／10年度美豆的农场价格有可能高于本年度。但年度均价在未来的1到2个年度仍倾向于下滑。     

Effect of Fermentation Bed with Spent Mushroom Substrate of Pleurotus eryngii on Weaning Piglets

[Objective] The paper was to investigate effects on fermentation bed temperature,growth performance,diarrhea rate and digestive en-zyme activity of weaning piglets by using spent mushroom substrate of Pleurotus eryngii as padding.[Method] A total of 120 weaning piglets(Duroc × Landrace ×Yorkshire) with average initial body weight of(8.0 ±0.5)kg were allocated to five dietary treatments in a randomized complete block design for 42 d,each of which was replicated three times with eight piglets per replicate(half male,half female).The padding for control group was(50% sawdust +50% rice husk);experimental group Ⅰ 100% spent mushroom substrate;experimental group Ⅱ(15% sawdust +15% rice husk +70% spent mushroom substrate);experimental group Ⅲ(25% sawdust +25% rice husk +50% spent mushroom substrate);experimental group Ⅳ(35% sawdust +35% rice husk +30% spent substrate).[Result] There was no significant difference in surface temperature of fermentation bed between experimental groups and control group(P0.05).Compared with the control group,the temperature under 20 cm of fermentation bed in ex-perimental groups I,Ⅱ and Ⅲ increased significantly(P0.05).Except for experimental group Ⅳ,other three experimental groups had higher aver-age daily gain(P0.05) and experimental group Ⅰ had higher average daily feed intake(P0.05) compared to the control group.The diarrhea rate and mortality of weaning piglets in experimental groups Ⅱ,Ⅲ and Ⅳ were significantly decreased compared with the control group(P0.05).Compared with the control group,other three experimental groups had higher digestive enzyme activity in duodenal contents except for experimental group Ⅳ(P0.05).[Conclusion] Spent mushroom substrate of P.eryngii can be used as fermentation bed padding,and the optimal proportion was experimental group Ⅲ.     

DNA Barcoding:An Effective Tool for Species Identification of Lepidopteran Oak Pests

[Objective] The paper was to improve the efficiency and accuracy of early forecast of Lepidopteran oak-infesting pests.[Method] DNA barcoding technique was established for quick species identification using mitochondrial cytochrome C oxidase subunit Ⅰ(COⅠ) as the standard gene.This barcoding technique was used to amplify and sequence genomic DNA samples from eggs and pupae of 11 species of Lepidopteran pests collected from oak.[Result] The DNA barcoding standard genes of 594-708 bp were determined from eggs and pupae of Lepidopteran insects.There were differences of 0-2 bases in DNA barcode sequences between conspecific eggs and pupae,with the sequence identity of 99.7%-100%.The average content of A,T,G and C of DNA barcode sequences from Lepidopteran insects were 30.7%,38.5%,14.9% and 15.9%,respectively.The obtained DNA barcode sequences had 91.4%-100% identity and 0-8.6% difference degree with GenBank-deposited DNA barcode sequences from organisms of the genetically-closest relationship.Among them,DNA barcode sequences from egg and pupa samples of 10 Lepidopteran insects(No.1-20) had 99%-100% identity and 0-1.0% difference degree with homologous sequences in GenBank database,while the remaining samples(No.21-22) had high difference degree(8.6%) with homologous sequences.[Conclusion] The established DNA barcoding technique is an effeetive tool for species identification of Lepidopteran pests using genomic DNA from eggs and pupae of Lepidopteran insects.     

喷雾干燥鱼浆蛋白粉营养成分分析

本试验对鱼粉加工废水鱼浆蛋白粉(经浓缩、水解、喷雾干燥得到的副产品)进行营养成分分析,其中粗蛋白质含量达64.08%,钙2.67%、磷1.12%,钙磷比例合理;各氨基酸含量分别为:赖氨酸3.29%,蛋氨酸0.94%,谷氨酸5.17%,牛磺酸2.50%。     

缺氧诱导因子-1α与肉鸡腹水综合征

缺氧诱导因子-1α(HIF-1α)是机体缺氧信号传导途径中一个关键的转录因子-缺氧诱导因子-1(HIF-1)的一个活性亚基,对人类和哺乳动物肺动脉高压的形成具有极其重要的病理生理作用。文章综述了HIF-1α与哺乳动物肺动脉高压发生发展的关系及其可能的作用机理与途径,对剖析HIF-1α在肉鸡腹水综合征发生发展中的作用机理具有一定的意义。