2020年宁夏吴忠市畜间包虫病流行病学调查

为了解宁夏吴忠市畜间包虫病流行范围及防治效果,科学规范指导下一阶段包虫病防治工作,2020年对吴忠市5个县(市、区)采用基本情况调查、实验室抽样检测等方法进行了畜间包虫病流行病学调查.共检测犬粪样品1861份,犬细粒棘球绦虫感染阳性率为0.75％;抽检羊脏器4027份,棘球蚴感染率为0.22％,有3个县区检出感染;检测...     

深入推进首都签约兽医工作的思考

首都签约兽医工作是首都官方兽医的配套制度和工作体系的有机组成部分,按照北京市布署,房山区认真调研、部署推进。本文对签约兽医模式与协检员模式进行了简单的比较,对推进首都签约兽医运行机制的关键点进行了分析,并提出了建议:分类管理"专职"和"兼职"签约兽医,细化"签约",建立并逐步强化签约兽医责任追溯机制,争取各级财政落实专职签约兽医福利待遇和兼职签约兽医发放津贴等。     

滨州市推广紫穗槐产业发展有关问题探讨

该文阐述了紫穗槐的特性,提出了滨州市推广种植紫穗槐的重要性和可行性,并进行了效益分析。     

Genomic differentiation with isolation by distance along a latitudinal gradient in the spotted-leg treefrog Polypedates megacephalus

The patterns of isolation by distance (IBD) entailing increased genetic differentiation among populations have aroused extensive concerns for evolutionary biologists. Although the IBD may act on spatial processes contributing to the genetic differentiation among populations in anuran species, the factors shaping the IBD of frogs among populations in natural systems are largely unknown. Here, we studied the genetic differentiation among six populations with 24 individuals of the spotted-leg treefrog along a latitudinal gradient (1860.31 km) based on 1020 single nucleotide polymorphisms from restriction site-associated DNA sequencing. The results showed that the genetic diversity differed significantly among populations and that the insular populations had higher genetic diversity than the mainland populations. Furthermore, we also found a significant genetic differentiation among populations (F_ST = 0.277) and no sign of inbreeding (F_UNI = −0.145). The IBD was detected for all populations, and a higher degree of the IBD was indicated when controlling for the effects of the isolation between Hainan and mainland populations caused by the Qiongzhou Strait. Our findings suggest that the form of the Qiongzhou Strait plays a key role in shaping the genetic diversity and population differentiation in treefrogs.     

氟对牛脾淋巴细胞膜ATP酶活性的影响

取健康牛脾脏,常规方法分离培养淋巴细胞,用不同浓度氟化钠（NaF）染毒24h。低渗溶胞法提取细胞膜,检测膜上ATP酶活性。结果显示,与对照组相比较,染氟组淋巴细胞膜ATP酶活性降低,组间差异显著或极显著。表明：氟能抑制牛脾淋巴细胞膜ATP酶活性。     

一例牙鲆淋巴囊肿病例的诊断及病毒MCP基因遗传进化分析

2019年1月,山东省某牙鲆鱼场出现疑似淋巴囊肿病例。患病鱼体表长有灰白色菜花样瘤状物,死亡率超过30%,给养殖场造成了较为严重的经济损失。为确定发病原因,对该鱼场病死鱼进行了病理组织学诊断和病毒检测,并对病毒MCP基因进行了序列测定及遗传变异分析。结果显示：眼观及组织学病变符合淋巴囊肿病的病变特征;所检测到的病毒与选定的参考毒株核苷酸和氨基酸同源性分别为78.7%~99.9%和86.8%~100%。MCP基因遗传进化分析显示,检测到的病毒与LCDV-C、JF00Kuma、JF03Yoshi、JF03GunNeA、JF、LCDV-K1等毒株同属于淋巴囊肿病毒基因II型,且与我国分离株LCDV-C有较高的同源性。经综合诊断,确定该流行病由淋巴囊肿病毒基因II型所致。该研究为该病的综合诊断及流行病学调查提供了新资料,为下一步进行该病毒相关分子生物学研究以及疫苗制备等提供了理论依据。     

Isolation and characterization of emerging subgroup J avian leukosis virus associated with hemangioma in egg-type chickens

Subgroup J avian leukosis virus (ALV-J), first isolated in 1989, predominantly causes myeloid leukosis (ML) in meat-type or egg-type chicken. Since 2006, the clinical cases of hemangioma rather than ML in commercial layer flocks associated with ALV-J have been reported, but it was still not clear whether the novel oncogenic ALV-J had emerged. We characterized SCAU-HN06 isolate of ALV-J from hemangioma in commercial Roman layers through animal experiment and full-length proviral genome sequence analysis. The SPF white leghorn egg-type chickens infected with SCAU-HN06 in ovo at day 11 of incubation showed an overall incidence of 56% hemangioma and 8% renal tumor throughout the 22-week trial, the mortality rate was 16%. Most genes of SCAU-HN06 isolate showed high nucleotide sequence identity to JS09GY6 which was isolated from Hy-Line Variety Brown layers suffering hemangioma. The 19-bp insertion in leader sequence and one key deletion in E element were the common features of SCAU-HN06 and JS09GY6. SCAU-HN06 and those ALV-Js associated with hemangioma, possibly recombinants of ALV-J and other avian retrovirus, may share the same ancestor.     

犬食盐中毒诊治1例

犬食盐中毒在临床病例中较为少见,原因在于不同动物对食盐的敏感性不同,鸡和猪最为敏感,而犬的肾脏有非常好的排泄功能,对食盐并不敏感。但养犬者饲养管理方法不当。也会造成犬食盐中毒。笔眷在宠物门诊学习期间。遇到一例犬食盐中毒的病例。经过快速诊断和及时有效的救治。病犬成功治愈。     

基于MODIS数据的伊犁河谷植被指数变化及其对气候的响应

利用2006-2014年主要生长季(5-9月)的MODIS遥感数据,分析了2006-2014年的年平均NDVI和生长季月NDVI变化规律。同时,收集整理同期伊犁河谷地区的气温与降水资料,并对NDVI与当月、2个月滑动、3个月滑动和5个月滑动的平均气温、降水值进行了相关性分析。结果表明,NDVI与气温、降水都存在一定的相关性,NDVI受当月平均气温的影响最大;NDVI与当月降水和滑动平均降水的相关性都很好,但3个月滑动平均降水对NDVI的影响最大,并且通过了0.01的极显著性检验。