通过财务稽核强化电力企业的内部控制

通过对财务稽核的意义、作用以及电力企业内部控制的基本要求,阐述财务稽核对强化企业内部控制的重要性,同时列举电力企业管理中容易出现的财务管理方面的漏洞,并依次作为问题进行讨论寻找解决的途径。     

基于DPSA算法的“单库-单站”水资源优化调度方法研究

提出了单座水库与单座补水泵站水资源优化调度的非线性数学模型,模型以年内各时段的缺(弃)水量的平方最小为目标函数,各时段水库供水量和泵站引提水量为决策变量,各时段水库库容为状态变量,以年调节水库可供水量、泵站年引提水总量、水库与补水泵站的联合运行准则(泵站闲时补库、忙时供水)等为约束条件,并以南水北调东线一期江苏境内"骆马湖-皂河站"为实例,采用动态规划逐次逼近法进行求解。结果表明,相同供水保证率时,50%、75%、95%来水频率下泵站补水量、缺水量、弃水量比常规调度均有减少。     

干旱地区膜下滴灌技术甜瓜种植模式试验研究

本文通过田间试验,探索干旱地区甜瓜由传统起垄滴灌种植变为平地滴灌种植的可行性。同时,探讨与滴灌相适宜的植株密度以及经济的滴灌带布置方式,以提高甜瓜种植的经济效益。本文可为类似地区推广平地滴灌技术提供借鉴。     

基于灰色关联法的小型农田水利工程建设项目绩效评价研究

运用灰色关联法原理,采用均方差法计算指标权重,根据近年来湖北省进行小型农田水利工程建设项目绩效评价的实际情况,建立了小型农田水利工程建设项目评价定性定量混合指标体系和模型,应用该模型和方法对湖北省2012年第二批20个小农水重点县工程建设项目进行了评价(作为研究,各县、市名以汉语拼音字头代替)。结果表明:LHK、SSS、ZJS、DJK、XTS等14个县为优秀,XCX、TMS、JSX等5个县为良好,CYX为一般,无及格等级的重点县。评价结果与实际基本相符。证明本文所提出的模型和方法可应用于实际,是一种快速便捷的评价工具。     

长株潭城市群土地利用变化现状及策略分析

文章通过对长株潭城市群土地利用现状剖析,结合相关的理论提出相应的策略：以循环经济理念指导土地利用,优化土地利用结构与布局。     

Cloning and Bioinformatic Analysis of L1 Gene of BPV from Guizhou Province

In order to study and analyze L1 gene of bovine papillomavirus（BPV）in Guizhou province,the L1 gene of BPV-GZ01 strain was amplified,cloned and sequenced using bioinformatic softwares and methods,and the secondary structure,tertiary structure,B-cell preponderant epitope,conserved domains analysis, transmembrane domain and signal peptide of L1 gene were predicted.The results showed that the length of L1 gene was 1 494 bp,encoding 497 amino acids.The L1 gene of BPV-GZ01 strain shared an amino acid identities of 98.6%,99.4%,98.4%,94.4% and 91.3%,and a nucleotide identities of 99.1%,99.8%,99.4%,87.6% and 82.8% with those of BPV2,BPV2-SW01,BPV2-AKS01,BPV13 and BPV1 strains,respectively.The results of phylogenetic tree analysis indicated that there was a close relationship between BPV-GZ01 and BPV2-SW01 strains.The prediction of secondary structure of L1 protein indicated that the random coil,extended strand and alphahelix took a higher percentage.The L1 protein was supposed contain 6 potential antigen epitopes.And no transmembrane domains and no signal peptide were found.The tertiary structure of L1 protein was curved spiral structure.These results provided a theoretical basis for immunologic diagnosis and further research of nucleic acid vaccine of BPV.     

Production and Identification of Clone Sheep Using Bone Marrow Mesenchymal Stem Cells as Donor Cell

In order to obtain the cloned sheep using bone marrow mesenchymal stem cells（BMSCs）as the donor cells,the BMSCs of sheep were chosen and reconstructed embryos were built to transfer.10 published microsatellite markers were chosen,and the DNA samples from clone sheep,donor cells and surrogate ewes were amplified,and the relationship of father-son（RCP）was analyzed using the Quantity One for genotyping.The results showed that the reconstructed embryos were successfully built for electric fusion using sheep BMSCs as nuclear donor,and making nuclear transplantation into enucleated mature oocytes of which the fusion rate was 80.62%.20 surrogate ewe were chosen to be implanted with the reconstructed embryos at morula stage by implant surgery,and 5 lambs were born and only 3 were survived.The genotype of cloned sheep was in line with the dornor cell and the RCP were more than 99.999%.In conclusion,the first clone sheep were obtained successfully by using BMSCs as a nuclear donor in this experiment.     

Effect of Aerosol OT and Paraformaldehyde Supplement on the Voluntary Intake,Digestion and Metabolism of Late-pregnant Ewe Fed with Ground Diet

The Small-tail Han ewes with 3 years old, (48.7±2.0)kg body weight, carring the multi-lamb gene, were fed with the same diet, mating after one month.18 pregnant ewes were selected and divided into 3 groups(groups 1(control group), 2 and 3).From 90 days of pregnancy, groups 2 and 3 were supplemented with aerosol OT(800 mg/kg) and paraformaldehyde(300 mg/kg), respectively, and the experiments of digestion and metabolism were carried on from 121 to 130 days of pregnancy to study the effect of aerosol OT and paraformaldehyde supplements on the voluntary intake, digestion and metabolism, lambing of late-pregnant ewes.The results showed that when fed with aerosol OT, the voluntary intake of dry matter was increased by 13.0%(P<0.01);The amount of digestion of DM, OM, CP, cellulose and GE was increased by 12.4%(P<0.05), 14.4%(P<0.01), 23.2%(P<0.01), 15.2%(P>0.05) and 27.4%(P<0.01), respectively;The retention of N, Ca and P was increased by 31.1%(P<0.01), 48.5%(P<0.01) and 31.9%(P<0.01), respectively;The serum triglyceride and free fatty acid content tended to decrease;but the apparent digestibility of the dietary nutrients were not significantly affected(P>0.05);The loss of ewe postpartum weight was reduced by 61.9%(P<0.01), a nest of lamb weight was increased by 4.2%(P>0.05).In the condition of feeding paraformaldehyde, the voluntary intake of dry matter was increased by 12.6%(P<0.01);The amount of digestion of DM, OM, CP, cellulose and GE was increased by 15.4%(P<0.01), 16.2%(P<0.01), 22.4%(P<0.01), 14.2%(P>0.05) and 29.0%(P<0.01), respectively;The retention of N, Ca and P was increased by 33.5%(P<0.01), 48.9%(P<0.01) and 36.3%(P<0.01), respectively;But the apparent digestibility of the dietary nutrients were no significantly affected(P>0.05);The serum β-hydroxybutrate and free fatty acid content tended to decrease;The loss of ewe postpartum weight was reduced by 57.1%(P<0.01), a nest of lamb weight was increased by 11.1%(P<0.05).This study showed that by feeding aerosol OT or paraformaldehyde, the intake, digestibility of Ca, P and N could be improved in late-pregnant ewe, the body weight loss, body fat mobilization could be reduced, and the birth weight of litter could be increased.     

濒危植物羽叶丁香组织培养

以濒危植物羽叶丁香的芽和种子为外植体,采用植物组织培养法,研究了不同消毒方法对无菌体系建立、不同浓度的蔗糖和激素组合对种子初代培养、不同基本培养基和激素组合对增殖和生根的影响,以期为羽叶丁香的组培快繁、种质资源保护和开发利用提供依据。结果表明:新枝为较适宜的外植体,适宜的消毒方式为75%酒精消毒30s,0.1%升汞消毒7min,最适增殖培养基为MS+5.0mg·L~(-1) 6-BA+0.01mg·L~(-1) IBA;最适种子初代培养基为MS+20g·L~(-1)蔗糖汁+3.0mg·L~(-1) 6-BA+0.10mg·L~(-1) IBA,最适增殖培养基为MS+7.0mg·L~(-1) 6-BA+0.05mg·L~(-1) IBA;最适生根培养基为WPM+2.0mg·L~(-1) IBA。     

景天属植物耐非生物胁迫研究进展

景天属植物作为园林绿化的重要植物材料已被广泛应用。了解其对非生物胁迫逆境的适应性,对在生态园林城市的建设和环境修复中景天品种的选择和利用具有重大意义。现综述了近年来我国在景天属植物耐高温、低温、干旱、涝、酸、盐、光和重金属等非生物胁迫研究方面的进展,对存在的问题及今后的发展方向提出了一些建议。