青海湖地区植物中常量营养元素含量特征

以青海湖地区各类植被中采集的46种主要植物样品，进行6种常量营养元素的分析测试，得到了区内主要植物常量营养元素的一些特征。为青海湖地区生态环境研究、草原建设、畜牧业发展等提供一些基础资料。     

母猪饲料中添加中药“泻康宁”对仔猪黄白痢的治疗试验

由埃希氏大肠杆菌引起的仔猪黄白痢会导致机体水分和电解质大量丧失 ,使机体衰弱甚至死亡 ,从而造成很大的经济损失。目前 ,大肠杆菌引起的仔猪黄白痢已成为影响仔猪发育和育成的重要疾病之一 ,成为养猪业的一大障碍。虽然应用抗生素、磺胺类药物和抗菌增效剂等治疗仔猪黄白痢有一定的疗效 ,但由于大肠杆菌具有很强的变异性 ,易产生抗药性而影响治疗效果 ,而且 ,有的药物还会在体内蓄积 ,给人类的健康带来危害 ,正因为如此 ,人们开始寻找新的无公害的药物。实验研究发现 ,中草药不仅能够促进畜禽生长发育和提高生产性能 ,调节动物机体的新陈…     

WTO协议的文体特征探析

应用现代文体学理论并结合语用学和认知语言学理论观点,尝试从字位与书写、词汇、句法层面描述和分析WTO英文协议的文体特征,希望对理解和翻译世界贸易法规和类似的经贸法规有所帮助。     

论贫困山区县的生态旅游开发--以湖南新化县为例

生态旅游作为实现旅游可持续发展的重要途径正受到全球范围内的认同和追随。新化县是湖南中部的一个贫困山区县，对新化县的生态旅游资源作了全面的评价，从资源、区位、宏观环境等多方面对新化县生态旅游开发可行性进行了深入的分析，并提出了一系列生态旅游的开发策略。     

供水工程影响下中国北方地区耕地后备资源开发潜力

灌溉水源是北方地区耕地后备资源能否有效开发的关键。当前,中国正在推动国家水网工程规划建设,为北方地区耕地后备资源开发提供了新的机遇。在以往研究基础上,该研究考虑北方地区已建、在建及规划的供水工程,基于自然适宜、利用高效、发展稳定三方面评价准则,结合三维魔方空间分类方法,开展了雨养情景和供水灌溉情景下耕地后备资源开发潜力分析。结果表明：雨养农业情景下,北方地区勉强适宜（Ⅱ级）、中度（Ⅲ级）和高度（Ⅳ级）适宜的耕地后备资源面积分别为2.9万、1.0万和0.6万km²。灌溉农业情景下,耕地后备资源面积明显增加,勉强适宜（Ⅱ级）、中度（Ⅲ级）和高度（Ⅳ级）适宜的耕地后备资源面积将分别达到5.4万、7.6万和6.5万km²,主要集中于新疆、内蒙古和甘肃3省（自治区）。该研究结果可为中国耕地后备资源开发利用及国家水网工程效益发挥提供参考。     

The rates of maize growth and lignin biosynthesis change after root-applied chalcone

The effects of root-applied chalcone at 0.15 mmol L⁻¹ on the growth and lignin biosynthesis in maize were investigated. The contents of 4-coumarate: CoA ligase (4CL, EC 6.2.1.12) substrates in maize shoots were increased more rapidly in the samples with chalcone application than in the control and the increase occurred at ≤ 3 h after the application (HAA). The lignin content was reduced by chalcone at ≤ 6 HAA. The shoot growth was suppressed by chalcone at ≤ 9 HAA. Consequently, the results suggest that chalcone suppressed maize growth by inhibiting monolignol biosynthesis.     

Cell proliferation and nestin expression in cortex and SEZ of MCAO rats

AIM:To explore the effect of brain ischemia injury on cell proliferation and nestin expression in cortex and subependymal zone (SEZ).METHODS:Using a local brain ischemia model(MCAO), BrdU positive cells of cortex and subependymal zone (SEZ), also nestin positive cells, were observed by immunohistochemistry.RESULTS:BrdU and nestin positive cells in SEZ of MCAO rats were obviously increased. In cortex, only nestin positive cells were observed.CONCLUSION:Neural stem cells in SEZ and cortex were activated after brain ischemia, it may be related with neural recovery after brain ischemia injury.     

and laminin on adhesion and proliferation of human hepatocellular carcinoma cell

AIM:To explore the effects of PMA(phorbol-12-myristate-13-acetate, a tumor promoter, mimicking the action of diacylglycerol on PKC)and laminin on the adhesion and the proliferation of human hepatocellular carcinoma cells, and provide a new clue to liver cancer treatment.METHODS:Human hepatocellular carcinoma cell line(BEL-7402)was used to identify the endogenous laminin and protein kinase C-α(PKC-α) expression, and the effects of laminin and PMA on the adhesion and the proliferation were also investigatedin vitro.RESULTS:By the effect of exogenous laminin, human hepatocellular carcinoma cell (BEL-7402) possessed endogenous laminin expression and increased the adhesion and the proliferation, which was showed the synergistic action by the effect of PMA in combination. By the action of PMA alone, the proliferation and the PKC-α expression increased by exogenous laminin were decreased, and the adhesion and the endogenous laminin expression were increased.CONCLUSIONS:The finding suggested that the adhesion and the proliferation of human hepatocellular carcinoma cell were closely related to the effects of endogenous or exogenous laminin, which were associated with cPKC-α activity. Therefore, the application of anti-laminin antibody in combination with PKC antagonist might be a new clue to find out the therapy for liver cancer.     

The application of atomic force microscope in clinic diagnosis of erythrocytes

AIM: To investigate the application of atomic force microscope (AFM) in clinic diagnosis. METHODS: Topographic images and some parameters of large range field and microstructures of erythrocytes in the blood of normal subjects, lung cancer and myelodisplastic syndrome (MDS) patients were examined by atomic force microscope. RESULTS: Many clear topographic images of many erythrocytes, single erythrocyte, and microstructure of erythrocyte membrane surface were obtained. Many erythrocytes in lung cancer patients were found to change into echinocytes. One erythrocyte had 10-20 protuberances, most of which, with a mean width of 589. 0 nm and a length of 646. 7 nm, were on the edge of cells. The protuberances on the center of echinocytes are lodged and embedded. The erythrocytes of MDS patients were biconcave in shape. Many apertures with different diameters of tens to hundreds nanometer appeared on the surface of cell membrane. CONCLUSIONS: AFM can be widely applied in clinic pathological inspection, including quantification of cells, obtainment and comparison of many parameters (such as diameter, thickness, volume, surface, surface area/volume ratio), observation of topograph of single cell, and observation and comparison of membrane surface microstructure of cells, and so on.     

Bone marrow-derived stromal cells and cartilage tissue engineering

Articular cartilage repair is one of the most challenging issues which remains to be resolved in clinic work. Discovering of bone marrow stromal cells (BMSC) and its application in tissue engineering provide new methods for the treatment of cartilage defects. High seeding density, appropriate cytokines and three-dimensional culture play important roles in the process of inducing BMSC differentiating into chondrocytes, suitable scaffold is also essential in reconstructing cartilage in vitro by methods of tissue engineering.