全文获取类型
收费全文 | 86333篇 |
免费 | 5090篇 |
国内免费 | 7662篇 |
专业分类
林业 | 6644篇 |
农学 | 4459篇 |
基础科学 | 3667篇 |
7688篇 | |
综合类 | 43102篇 |
农作物 | 6622篇 |
水产渔业 | 4307篇 |
畜牧兽医 | 12453篇 |
园艺 | 6638篇 |
植物保护 | 3505篇 |
出版年
2024年 | 693篇 |
2023年 | 1741篇 |
2022年 | 3754篇 |
2021年 | 3820篇 |
2020年 | 3547篇 |
2019年 | 3526篇 |
2018年 | 2501篇 |
2017年 | 4106篇 |
2016年 | 2642篇 |
2015年 | 4116篇 |
2014年 | 4417篇 |
2013年 | 5279篇 |
2012年 | 7448篇 |
2011年 | 7447篇 |
2010年 | 6964篇 |
2009年 | 6202篇 |
2008年 | 6432篇 |
2007年 | 6017篇 |
2006年 | 4886篇 |
2005年 | 3999篇 |
2004年 | 2444篇 |
2003年 | 1539篇 |
2002年 | 1759篇 |
2001年 | 1485篇 |
2000年 | 1349篇 |
1999年 | 495篇 |
1998年 | 66篇 |
1997年 | 41篇 |
1996年 | 20篇 |
1995年 | 35篇 |
1994年 | 31篇 |
1993年 | 35篇 |
1992年 | 39篇 |
1991年 | 29篇 |
1990年 | 14篇 |
1989年 | 10篇 |
1987年 | 19篇 |
1986年 | 18篇 |
1985年 | 3篇 |
1981年 | 5篇 |
1965年 | 3篇 |
1964年 | 4篇 |
1963年 | 4篇 |
1962年 | 17篇 |
1961年 | 5篇 |
1959年 | 3篇 |
1958年 | 3篇 |
1957年 | 11篇 |
1956年 | 32篇 |
1955年 | 17篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
1.
2.
陈俊梅 《农业装备与车辆工程》2009,(9):16-18
介绍了成卷工艺对棉卷结构的影响以及棉卷成卷过程中起泡现象与准备工艺的关系.分析了棉卷成卷工艺过程中起泡过程和起泡原因,提出了防止起泡的措施. 相似文献
3.
4.
5.
应用现代文体学理论并结合语用学和认知语言学理论观点,尝试从字位与书写、词汇、句法层面描述和分析WTO英文协议的文体特征,希望对理解和翻译世界贸易法规和类似的经贸法规有所帮助。 相似文献
6.
The effects of root-applied chalcone at 0.15 mmol L−1 on the growth and lignin biosynthesis in maize were investigated. The contents of 4-coumarate: CoA ligase (4CL, EC 6.2.1.12) substrates in maize shoots were increased more rapidly in the samples with chalcone application than in the control and the increase occurred at ≤ 3 h after the application (HAA). The lignin content was reduced by chalcone at ≤ 6 HAA. The shoot growth was suppressed by chalcone at ≤ 9 HAA. Consequently, the results suggest that chalcone suppressed maize growth by inhibiting monolignol biosynthesis. 相似文献
7.
AIM:To explore the effects of PMA(phorbol-12-myristate-13-acetate, a tumor promoter, mimicking the action of diacylglycerol on PKC)and laminin on the adhesion and the proliferation of human hepatocellular carcinoma cells, and provide a new clue to liver cancer treatment.METHODS:Human hepatocellular carcinoma cell line(BEL-7402)was used to identify the endogenous laminin and protein kinase C-α(PKC-α) expression, and the effects of laminin and PMA on the adhesion and the proliferation were also investigatedin vitro.RESULTS:By the effect of exogenous laminin, human hepatocellular carcinoma cell (BEL-7402) possessed endogenous laminin expression and increased the adhesion and the proliferation, which was showed the synergistic action by the effect of PMA in combination. By the action of PMA alone, the proliferation and the PKC-α expression increased by exogenous laminin were decreased, and the adhesion and the endogenous laminin expression were increased.CONCLUSIONS:The finding suggested that the adhesion and the proliferation of human hepatocellular carcinoma cell were closely related to the effects of endogenous or exogenous laminin, which were associated with cPKC-α activity. Therefore, the application of anti-laminin antibody in combination with PKC antagonist might be a new clue to find out the therapy for liver cancer. 相似文献
8.
AIM: To investigate the application of atomic force microscope (AFM) in clinic diagnosis. METHODS: Topographic images and some parameters of large range field and microstructures of erythrocytes in the blood of normal subjects, lung cancer and myelodisplastic syndrome (MDS) patients were examined by atomic force microscope. RESULTS: Many clear topographic images of many erythrocytes, single erythrocyte, and microstructure of erythrocyte membrane surface were obtained. Many erythrocytes in lung cancer patients were found to change into echinocytes. One erythrocyte had 10-20 protuberances, most of which, with a mean width of 589. 0 nm and a length of 646. 7 nm, were on the edge of cells. The protuberances on the center of echinocytes are lodged and embedded. The erythrocytes of MDS patients were biconcave in shape. Many apertures with different diameters of tens to hundreds nanometer appeared on the surface of cell membrane. CONCLUSIONS: AFM can be widely applied in clinic pathological inspection, including quantification of cells, obtainment and comparison of many parameters (such as diameter, thickness, volume, surface, surface area/volume ratio), observation of topograph of single cell, and observation and comparison of membrane surface microstructure of cells, and so on. 相似文献
9.
Articular cartilage repair is one of the most challenging issues which remains to be resolved in clinic work. Discovering of bone marrow stromal cells (BMSC) and its application in tissue engineering provide new methods for the treatment of cartilage defects. High seeding density, appropriate cytokines and three-dimensional culture play important roles in the process of inducing BMSC differentiating into chondrocytes, suitable scaffold is also essential in reconstructing cartilage in vitro by methods of tissue engineering. 相似文献
10.
ZHANG Li-rong XIANG Peng XIA Wen-jie CHEN Zhen-guang ZHANG Xiu-ming LI Yan LI Shu-nong 《园艺学报》2002,18(8):896-899
AIM: To investigate the effects of PD98059 on the differentiation from mesenchymal stem cells to osteoblasts.METHODS: hMSC were separated from human marrow and expanded in cuture medium. hMSC were induced with dexamethasone, β-glycerophosphate, vitamin C which acted as osteoblast differentiation inducer. PD98059 was added into the osteoblasts induction medium. The cells were assayed with cell morphology, alkaline phosphatase (AP) activity and calcium deposition. RESULTS: The isolated cultured MSC comprised a single phenotypic population and displayed a fibroblast-like morphology. After induced with osteoblasts induction medium, the cells showed changes in cell morphology from spindle-shape to cuboidal and polygonal. The AP activity increased gradually and reached the peak in 12 days, then decreased. Many scattered tangerice calcium nodes were observed. PD 98059 significantly inhibited AP activity and calcium deposition in a dose-dependent manner. A striking observation of the present study was that a few adipocytes appeared in cultures that were treated with PD 98059 and osteogenic differentiation medium. CONCLUSION: These results indicated that osteogenic diferentiation from the hMSCs was related to the activation of the ERK. 相似文献