广西蚕区主推家蚕品种在不同区域及不同批次饲养的适应性调查

将广西壮族自治区主推的3对家蚕品种分别在桂西北、桂中和桂南几个具有地域代表性蚕区的不同养蚕批次进行适应性饲养试验,调查不同品种的蚕茧产量和品质,以期通过家蚕品种最好的生产应用布局来提高广大蚕农的经济收益.根据试验结果建议:那坡、环江蚕区每年第1、2、3、10、11批次对饲养家蚕品种的选择顺序为桂蚕2号、两广二号或桂蚕N...     

基于《耕地质量等级》的靖远县耕地质量等级评价

基于《耕地质量等级》标准,按照黄土高原区耕地质量等级评定指标体系,利用县域耕地资源管理信息系统建立耕地质量等级信息系统数据库,运用空间分析、层次分析、模糊数学和综合指数等方法对靖远县耕地质量等级进行综合评价.结果表明:靖远县耕地受地貌、地形和水文地质条件影响等级高低出现了明显的区域分布规律,从北到南,依次为十等地到三等地,面积占比分别为1.51％、23.51％、41.55％、15.92％、10.31％、3.87％、2.34％、0.99％.耕地质量平均等级为7.64等,耕地主要利用类型由旱地和砂田逐渐向菜地和水田过渡.全县耕地土壤有机质、有效磷、速效钾平均含量为11.41 g/kg、16.55 mg/kg、178.03 mg/kg,均属于中等水平.全氮为0.70 g/kg,属于低等水平.该评价较准确地反映了靖远县耕地质量等级现状,可对耕地质量的监测和保护提供了参考依据.     

硒对鸭镉中毒保护效应的研究

６６只３日龄天府肉鸭随机分为６组,其中一组每日上、下午灌服蒸馏水作为试验对照组,另５组每日早晨灌以１／２０ＤＬ５０（１４．４７ｍｇ／ｋｇ体重）的氯化镉水溶液作试验组,试验Ⅰ－Ⅳ组每日下午分别再灌以３．３９、１．６９、０．８５、０．４２ｍｇ／ｋｇ的硒,第Ⅴ组灌服蒸馏水。试验期６周,其发病率和死亡率分另为１０／１１、９／１１、１０／１１、１０／１１、１１／１１、和４／１１、３／１１、２／１１、４／１１     

Green tea polyphenols supplementation alters immunometabolism and oxidative stress in dairy cows with hyperketonemia

Peripartal cows often experience negative energy balance, and are therefore prone to suffering from metabolic diseases such as hyperketonemia, which causes financial losses in dairy farms. This study aimed to investigate the effect of green tea polyphenol (GTP) supplementation during the periparturient period on production performance, oxidative stress and immunometabolism in dairy cows with hyperketonemia. One hundred Holstein cows were assigned to GTP (0.2 g/kg DM; n = 50) or control (without GTP; n = 50) group based on body weight, previous milk yield, and parity on d 15 before expected parturition. Subsequently, 10 cows with hyperketonemia were selected from each group, according to blood β-hydroxybutyric acid (BHBA) concentration between 1.2 and 2.9 mmol/L from 2 to 3 d postpartum. All cows were fed a close-up diet and a lactation diet with or without GTP supply from 15 d prepartum until 30 d postpartum. Milk and blood samples were obtained from 20 cows selected with hyperketonemia on 10, 20, and 30 d postpartum. Compared with control cows, greater milk yield and lower somatic cell count were observed in GTP cows. The GTP group had lower concentrations of BHBA, free fatty acids, cholesterol, triglyceride, reactive oxygen species, malondialdehyde, and hydrogen peroxide, greater concentrations of glucose, lower activities of aspartate aminotransferase, alanine aminotransferase, and glutamyl transpeptidase, alongside greater activities of superoxide dismutase, glutathione peroxidase, and total antioxidant capacity. Additionally, GTP supplementation up-regulated concentrations of interleukin-6 and interleukin-10, but down-regulated concentrations of tumor necrosis factor-α, interleukin-1β, interleukin-2, interleukin-8, and interferon-γ in plasma. Greater concentrations of plasma immunoglobulin G were also detected in the GTP group. Overall, the data suggested that GTP supplementation from 15 d prepartum to 30 d postpartum improved the milk yield and health status in cows with hyperketonemia during early lactation.     

Pharmacokinetics of sanguinarine,chelerythrine, and their metabolites in broiler chickens following oral and intravenous administration

Sanguinarine (SA) and chelerythrine (CHE) are the main active components of the phytogenic livestock feed additive, Sangrovit®. However, little information is available on the pharmacokinetics of Sangrovit® in poultry. The goal of this work was to study the pharmacokinetics of SA, CHE, and their metabolites, dihydrosanguinarine (DHSA) and dihydrochelerythrine (DHCHE), in 10 healthy female broiler chickens following oral (p.o.) administration of Sangrovit® and intravenous (i.v.) administration of a mixture of SA and CHE. The plasma samples were processed using two different simple protein precipitation methods because the parent drugs and metabolites are stable under different pH conditions. The absorption and metabolism of SA following p.o. administration were fast, with half‐life (t_1/2) values of 1.05 ± 0.18 hr and 0.83 ± 0.10 hr for SA and DHSA, respectively. The maximum concentration (C_max) of DHSA (2.49 ± 1.4 μg/L) was higher that of SA (1.89 ± 0.8 μg/L). The area under the concentration vs. time curve (AUC) values for SA and DHSA were 9.92 ± 5.4 and 6.08 ± 3.49 ng/ml hr, respectively. Following i.v. administration, the clearance (CL) of SA was 6.79 ± 0.63 (L·h^?1·kg^?1) with a t_1/2 of 0.34 ± 0.13 hr. The AUC values for DHSA and DHCHE were 7.48 ± 1.05 and 0.52 ± 0.09 (ng/ml hr), respectively. These data suggested that Sangrovit® had low absorption and bioavailability in broiler chickens. The work reported here provides useful information on the pharmacokinetic behavior of Sangrovit® after p.o. and i.v. administration in broiler chickens, which is important for the evaluation of its use in poultry.     

宿主域扩大的重组救活昆虫杆状病毒表达载体的构建及外源基因的表达

通过克隆的家蚕核多角体病毒解旋酶基因DNA与重组救活可线性化的苜蓿尺蠖核多角体病毒基因工程载体病毒 (BacPAK6 )DNA在昆虫细胞中发生重组 ,经累代筛选获得了既可感染家蚕又可感染秋粘虫细胞Sf 2 1的宿主域扩大的昆虫杆状病毒表达载体 (HyBacPAK6 )。HyBacPAK6DNA经Bsu36Ⅰ酶切后与含植酸酶基因的转移载体pVL1393 phy在家蚕细胞中重组后 ,通过蓝白斑筛选发现重组率可达 90 %以上。然而以杂交病毒为载体的外源基因表达量仅为以BmNPV为载体病毒的表达量的 2 0 %左右。分析认为HyBacPAK6可用于表达一些对蚕体有害的外源基因。     

Design of the dynamic safety management system for the confidential carrier based on RFID

针对当前涉密载体的管理现状,详细分析了涉密载体管理过程中存在的安全隐患,并基于RFID技术和数据库技术,提出了涉密载体动态安全管理系统设计方案.该系统包含涉密载体的电子标识、库存安全、借用审批、动态跟踪、动用查询和销毁六个子系统,可实现涉密载体的全生命周期管理.对涉密载体管理单位有着非常好的参考价值和现实意义.     

广西野生动物非法利用和走私的种类初步调查

广西独特的地理位置使得广西成为全国野生动物贸易的集散地之一。为了解广西野生动物的走私种类及其季节性变化,作者统计了广西2007～2008年已破获的非法利用和走私野生动物案件涉及的种类及其季节性变化。结果表明2007和2008年分别查获案件54起和61起。野生动物走私和非法案件中涉及种类共有71种,隶属15目33科,其中两栖类2种,占所有种数的2.8%;爬行类27种（蜥蜴类8种、龟鳖类5种、鳄类1种、蛇类13种）,占38.0%;鸟类25种,占35.2%;哺乳类17种,占23.9%。2007和2008年查获的非法利用和走私野生动物分别是56种和32种,非法利用或走私的种类主要是珍稀濒危的种类,数量大,种类多。广西的野生动物走私和非法利用需要引起关注。     

小肽的吸收与转运及其营养作用

随着动物营养学研究的发展,蛋白质营养的研究也在逐步深入,由原来的粗蛋白营养发展到氨基酸营养,进一步发展到现在的小肽营养。本文就小肽的吸收、转运、营养及其在动物生产中的应用,以及目前小肽研究领域中存在的问题等方面进行阐述。     

仔猪产气荚膜梭菌肠毒血症病原诊断及疫苗研究IV．肠毒血症C型疫苗生产用菌种的确定

试验证明用单一菌株C59-2生产C型产气荚膜梭菌肠毒血症干粉灭活疫苗,其效力可以达到《规程》要求,并且简化了生产工艺,降低了生产成本,提高了生产效率。疫苗免疫的兔血清可分别中和原来3个制苗用菌株所产生的外毒素。