Implantation of canine umbilical cord blood-derived mesenchymal stem cells mixed with beta-tricalcium phosphate enhances osteogenesis in bone defect model dogs

This study was performed to evaluate the osteogenic effect of allogenic canine umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) mixed with beta-tricalcium phosphate (β-TCP) in orthotopic implantation. Seven hundred milligrams of β-TCP mixed with 1 × 10⁶ UCB-MSCs diluted with 0.5 ml of saline (group CM) and mixed with the same volume of saline as control (group C) were implanted into a 1.5 cm diaphyseal defect and wrapped with PLGC membrane in the radius of Beagle dogs. Radiographs of the antebrachium were made after surgery. The implants were harvested 12 weeks after implantation and specimens were stained with H&E, toluidine blue and Villanueva-Goldner stains for histological examination and histomorphometric analysis of new bone formation. Additionally, UCB-MSCs were applied to a dog with non-union fracture. Radiographically, continuity between implant and host bone was evident at only one of six interfaces in group C by 12 weeks, but in three of six interfaces in group CM. Radiolucency was found only near the bone end in group C at 12 weeks after implantation, but in the entire graft in group CM. Histologically, bone formation was observed around β-TCP in longitudinal sections of implant in both groups. Histomorphometric analysis revealed significantly increased new bone formation in group CM at 12 weeks after implantation (p < 0.05). When applied to the non-union fracture, fracture healing was identified by 6 weeks after injection of UCB-MSCs. The present study indicates that a mixture of UCB-MSCs and β-TCP is a promising osteogenic material for repairing bone defects.     

Bending creep performances of three-ply cross-laminated woods made with five species

To improve the performance of cross-laminated woods, 30 types of three-ply parallel-laminated and cross-laminated woods were prepared from five species with various densities and shear compliances in cross section, and their bending creep performances were investigated on the basis of our previous research in cross-laminated wood made with sugi (Japanese cedar). The creep deformation perpendicular to the grain was decreased by cross laminating. The creep deformation perpendicular to the grain of parallel-laminated woods (P_⊥ type), that perpendicular to the grain of face laminae of cross-laminated woods (C_⊥ type), and also that parallel to the grain of face laminae of cross-laminated woods (C_║ type) tended to decrease with increasing density of species used for perpendicular-direction lamina. It was found that the extent of the decrease was greater in creep deformation than in initial deformation. The degrees of anisotropy for both deformations of laminated wood were markedly decreased by cross laminating. The extent of the decrease was much greater in creep deformation than in initial deformation and considerably smaller in buna with higher density than in sugi with lower density. The measured values of initial deformation and creep deformation of C_⊥ type were almost equal to the calculated values obtained from the measured values of parallel-laminated woods, whereas the measured values of both deformations of C_║ type were much greater than their calculated values and increased markedly with increasing shear compliance in cross section of perpendicular-direction lamina used for core. The ratios of the average of measured values to the calculated value of C_║ type ranged from 1.05 (katsura) to 1.50 (sugi) in initial deformation and from 1.30 (katsura) to 3.69 (sugi) in creep deformation. This result can be explained as the effect of deflection caused by shear force.     

Static bending strength performances of cross-laminated woods made with five species

Thirty types of three-ply parallel- and cross-laminated woods were prepared from five species, and their static bending strength performance were investigated. The modulus of elasticity (MOE), proportional limit stress, and modulus of rupture (MOR) perpendicular to the grain were increased by cross-laminating, and the extent of the increase increased with decreasing density of the species. The measured values of MOE parallel and perpendi-cular to the grain of parallel-laminated woods and perpendicular to the grain of face laminae of cross-laminated woods were approximately equal to those calculated from true MOEs of individual laminae. However, the MOE parallel to the grain of face laminae of cross-laminated woods was much lower than the calculated MOE owing to the effect of the deflection caused by shear force on the MOE. The percentage of deflection caused by shear force versus total deflection (Y _s) showed high values, from 16.1% (buna) to 40.5% (sugi), and it decreased linearly with increasing shear modulus in the cross section of the core. In addition, there was an extremely high positive correlation between the MOR and the measured MOE parallel to the grain of face laminae of cross-laminated woods. The MOR was also highly dependent on the shear modulus in cross section of the core.Part of this paper was presented at the 50th Annual Meeting of the Japan Wood Research Society, Kyoto, April 2000     

Derivation and application of an equation for calculating shear modulus of three-ply laminated material beam from shear moduli of individual laminae

In a detailed study of the relation between the deflection caused by shear force and the constitution of a laminated material beam, we derived an equation for calculating the shear modulus of a laminated material beam from the shear moduli of individual laminae. The validity of the derived equation was investigated using crosslaminated wood beams made with five species. The calculated shear moduli parallel to the grain of face laminae ranged from 48.3 MPa to 351 MPa, while those perpendicular to the grain of face laminae ranged from 58.0 MPa to 350 MPa. The calculated shear moduli increased markedly with increasing shear modulus in a cross section of perpendicular-direction lamina of a cross-laminated wood beam. The calculated apparent modulus of elasticity (MOE) of cross-laminated wood beams agreed fairly well with the measured apparent MOE values. This fact indicated that the apparent MOE of cross-laminated wood beam was able to be calculated from the true MOE values and shear moduli of individual laminae. The percentage of deflection caused by shear force obtained from the calculated apparent MOE (Y _sc) was close to that obtained from the measured apparent MOE (Y _s) and there was a high correlation between both values. From the above results, it was concluded that the derived equation had high validity in calculation of shear modulus of a cross-laminated wood beam.