花生蛋白的研究与开发

从花生蛋白的营养价值、功能特性、改性,以及在食品中的应用等方面,综述花生蛋白的应用现状,阐述了花生蛋白的优势及其潜力所在,并针对我国在花生蛋白加工利用方面的欠缺提出了几点建议。     

农药使用存在的问题及对策

分析生产实践中农药使用存在的问题,提出科学的应对措施,确保农产品安全无害化生产,实现高产、优质、高效的目标。     

粘虫色板防治菇蚊菇蝇的研究

为了给粘虫色板在食用菌害虫防治中的应用技术方案制定提供科学的依据,进行了对5种不同颜色的粘虫色板对菇蚊菇蝇的诱杀效果比较研究,进一步对粘虫色板的悬挂高度、悬挂密度、悬挂时间以及在菇房中应用粘虫色板的防效果和对食用菌产量的影响评价等方面研究。研究结果表明不同颜色的粘虫色板对食用菌菇蚊菇蝇的诱杀效果有显著差异,其中黄色粘虫色板对菇蚊菇蝇诱杀效果最佳;利用黄色粘虫色板悬挂在菇房中菌墙的上方30cm左右,晚间结合灯光诱杀效果最佳;处理菇房的菌袋受害率降低了14.7%,挽回产量损失达11.0%,防治效果显著。     

非洲猪瘟病毒LNA引物PCR检测方法的建立及优化

为了建立一种便捷、快速且精准的非洲猪瘟病毒的检测方法,本研究根据GenBank中公布的ASFV p72基因序列,设计了一对特异性引物,并对引物中的适当碱基进行锁核酸修饰,通过优化退火温度、引物浓度,建立了非洲猪瘟病毒的锁核酸修饰引物PCR检测方法。结果表明,该方法具有良好的敏感性和特异性,检测灵敏度可以达到3×10¹ copies/uL,比常规引物PCR方法的灵敏度提高了100倍,比real-time PCR方法的灵敏度提高了10倍,对猪瘟病毒、猪圆环病毒2型、猪伪狂犬病毒等病原基因组均无扩增,特异性良好。72份临床样品的检测结果与OIE推荐的qPCR方法检测结果一致,符合率为100%。本研究成功建立了非洲猪瘟病毒的LNA引物PCR检测方法,方法的特异性强、敏感性高,操作简单,为非洲猪瘟病毒的检测提供了一种新的、更加敏感的检测技术。     

基于VC++6.0实现图像直方图统计的研究

应用Matlab仿真工具进行图像处理算法的实现,存在运行效率低,且可移植性和实用性均不太理想。利用VC++程序,研究对象为黄瓜叶子,通过编程实现了图像直方图的统计。     

Effect of biochar amendment on soil‐silicon availability and rice uptake

Rice growth and its resistance to pests had been often constrained by soil‐silicon (Si) availability. The purpose of this study was to assess the potential of biochar soil amendment (BSA) to improve Si availability in paddy soils. A cross‐site field trail with BSA was conducted in six locations with different climatic and crop‐production conditions across S China. Plant‐available Si content before field‐trials establishment and after rice harvest, as well as Si content in rice shoot were determined. Varying with site conditions, plant‐available Si content of soil was observed to increase significantly with BSA in most sites. Significant increase in rice shoot Si was detected in four out of the six sites, which was well correlated to the concurrent increase in soil pH under BSA treatment. This study demonstrates an important role of BSA to improve Si availability and uptake by rice mainly through increasing soil pH of the acid and slightly acid rice soils.     

Cloning and expression analysis of PRL and PRLR genes in black Muscovy duck

ABSTRACT

1. Prolactin hormone (governed by the PRL gene) is secreted by the anterior pituitary of animals, which combines with its receptor (prolactin receptor, PRLR) to act on target cells. Both PRL and PRLR are mainly associated with reproductive performance. The genetic mechanism of nesting in poultry is not yet clear, and so the aim of the current study was to determine expression patterns of PRL and PRLR at different times across the breeding stages of black Muscovy ducks.

2. In this study, the CDS regions of PRL and PRLR were determined by RACE sequencing. The expression levels of PRL and PRLR in the pituitary, ovary and uterus from the black Muscovy duck were compared and analysed during the pre-laying, laying and nesting periods.

3. The results showed that PRL and PRLR are highly homologous in a variety of poultry species. The expression of the PRL gene in the pituitary was the highest, which was significantly higher than seen in the ovary and uterus. This trend ran through the entire prenatal period, i.e. the laying period and the nesting period. The expression level of the PRLR gene in the pituitary and ovary was generally low, and expression in the uterus was the highest. There was no significant difference in expression of the PRLR gene between pituitary and ovary during different periods, but the expression level of the PRLR gene in the uterus reached its highest level during the nesting stage, which was significantly higher than seen in the early laying period.     

生态环境对海岛棉纤维微观结构影响的研究

本文对海岛棉新海3号和88—346于1991、1992年,分别在生态条件有明显差异的新疆库尔勒、阿拉尔、莎车地区进行试验。结果表明:(1)生态环境影响纤维微观结构.环境条件对不同结构参数的影响不同,且因品种而异:(2)不同品种的微观结构参数对生态环境表现不同的适应性,但都有维持各自特征值的趋势;(3)品种内存在着取向参数与HVI比强度成正相关现象,这种关系同品种间已有的分析结果不同。     

试论传统音乐在经济发展中的传承

本文阐述了中国传统音乐的历史、发展和现状，具体分析了经济发展和传统音乐的矛盾提出了切实有效的保护、传承中国传统音乐的措施，并展望了中国传统音乐的发展前景。     

进口球根花卉携人线虫营养类群及数量检测

为了检测进口球根花卉种球携带的线虫，尤其是检疫名录之外的植物线虫。本研究首次采用本实验室设计的线虫7d自然游离分离法对进口花卉种球所携带的线虫进行了数量检测和营养类群归类。结果表明：1）从荷兰和新西兰进口的百合、郁金香等6种花卉种球中检出的线虫共涉及3目10科12属．其中非植物线虫分属1目3科4属，检疫名录以外的植物线虫数量占前3位的属有拟滑刃属（Aphelenchoides）、滑刃属（Aphelenchus）和短体属（Pratylenchus），分别占植物线虫总数的77．1％、16．4％和2．9％；2）新西兰进口百合种球中检测出的拟滑刃属线虫数量（426条／10球）为荷兰进口品种的3．5倍（123条／10球）。3）上述两国进口的百合种球中有3个品种携带潜在危险性线虫——根结属线虫，但数量较少，平均2～7条／10球．同时还携带非植物线虫，分属于盆咽属、拟丽突属、头叶属和小杆属。数量在50～300条／10球之间。