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71.
María Teresa Tejedor-Junco Margarita González-Martín Estefanía Bermeo-Garrido Rebeca Villasana-Loaiza Elena Carretón-Gómez 《Veterinary research communications》2018,42(3):227-232
Doxycycline is an antibiotic that, in addition to the classic antibacterial use, is also prescribed to fight parasitic diseases, like heartworm disease in dogs. Despite the concern that the overuse of this antibiotic may decrease susceptibility of clinically important bacteria, the consequences of the prolonged doxycycline therapy in heartworm-infected dogs have never been studied before. We have analyzed the impact of this therapy on Staphylococcus aureus and Enterococcus antimicrobial resistance. In this study, 17 heartworm-infected dogs (10 that had completed the doxycycline treatment and 7 dogs that had not yet begun) were included. Twenty-four isolates of Staphylococcus aureus were obtained from two locations of each dog. After treatment, 73.3% of isolates were resistant to at least one antibiotic but only 22.2% of isolates before treatment. Most of doxycycline resistant isolates were obtained from dogs that have received treatment. Erythromycin resistance or intermediate susceptibility was detected in 45.6% of isolates, most of them from dogs after treatment. For Enterococci, 48 isolates were obtained from fecal samples (25 before treatment and 23 after treatment). Before treatment, 32% of isolates were resistant at least to one antibiotic while after, this data increase up to 65%. Comparing isolates before and after treatment, a clear increase in resistance to doxycycline (12% against 21.74%) and erythromycin (20% against 39.13%) was observed. Although the present work is a preliminary research, the results encourages the development of further studies to determinate the effect of prolonged doxycycline therapy on antimicrobial resistance. 相似文献
72.
Tatsuya Nakayama Michio Jinnai Ryuji Kawahara Khong Thi Diep Nguyen Nam Thang Tran Thi Hoa Le Kieu Hanh Pham Ngoc Khai Yoshinori Sumimura Yoshimasa Yamamoto 《Tropical animal health and production》2017,49(1):31-37
Reports of livestock infections with extended-spectrum beta-lactamase-producing Escherichia coli (ESBL-E) are increasing. Based on interviews conducted over a 6-month period, we found that veterinarians in the Vietnamese province of Thai Binh prefer to prescribe colistin-based drugs (CBD) in chicken farms. We aimed to clarify whether CBD use selects for strains of colistin-resistant ESBL-E. With the cooperation of seven local households, we detected ESBL-E in chickens’ feces after treating chickens with CBD. Phylogenetic groupings and the presence of CTX-M/AmpC genes were determined, and the multi-antibiotic susceptibility of isolates was analyzed. Our results showed that ESBL-E presented in seven chickens’ feces from two households. Seventy-two percent of ESBL-E isolates harbored CTX-M9 and the phylogenetic group A; the colistin minimum inhibitory concentration (MIC) of all isolated ESBL-E ranged from 0.064 to 1 μg mL?1. Moreover, ESBL-E isolates were used to experimentally select for colistin resistance, and the effect of commercial CBD on ESBL-E was investigated. The results showed that an ESBL-E strain with a colistin MIC of 4 μg mL?1 was able to grow in media with CBD. Although CBD treatment was effective, in vitro experiments demonstrated that ESBL-E can easily acquire colistin resistance. Therefore, restrictions on colistin use are necessary to prevent the emergence of colistin-resistant bacteria. 相似文献
73.
Ragnhild J Bager Egle Kudirkiene Isabelle da Piedade Torsten Seemann Tine K Nielsen Susanne E Pors Andreas H Mattsson John D Boyce Ben Adler Anders M Bojesen 《Veterinary research》2014,45(1)
The Gram-negative bacterium Gallibacterium anatis is a major cause of salpingitis and peritonitis in commercial egg-layers, leading to reduced egg production and increased mortality. Unfortunately, widespread multidrug resistance and antigenic diversity makes it difficult to control infections and novel prevention strategies are urgently needed. In this study, a pan-genomic reverse vaccinology (RV) approach was used to identify potential vaccine candidates. Firstly, the genomes of 10 selected Gallibacterium strains were analyzed and proteins selected on the following criteria; predicted surface-exposure or secretion, none or one transmembrane helix (TMH), and presence in six or more of the 10 genomes. In total, 42 proteins were selected. The genes encoding 27 of these proteins were successfully cloned in Escherichia coli and the proteins expressed and purified. To reduce the number of vaccine candidates for in vivo testing, each of the purified recombinant proteins was screened by ELISA for their ability to elicit a significant serological response with serum from chickens that had been infected with G. anatis. Additionally, an in silico prediction of the protective potential was carried out based on a protein property prediction method. Of the 27 proteins, two novel putative immunogens were identified; Gab_1309 and Gab_2312. Moreover, three previously characterized virulence factors; GtxA, FlfA and Gab_2156, were identified. Thus, by combining the pan-genomic RV approach with subsequent in vitro and in silico screening, we have narrowed down the pan-proteome of G. anatis to five vaccine candidates. Importantly, preliminary immunization trials indicated an in vivo protective potential of GtxA-N, FlfA and Gab_1309.
Electronic supplementary material
The online version of this article (doi:10.1186/s13567-014-0080-0) contains supplementary material, which is available to authorized users. 相似文献74.
Ouédraogo-Koné S Kaboré-Zoungrana CY Ledin I 《Tropical animal health and production》2008,40(2):155-164
Foliage of Afzelia africana, Pterocarpus erinaceus and Khaya senegalensis, from 10 trees per species, was collected every two weeks during the late dry, rainy and cool season to determine the seasonal
effects on chemical composition. Fifteen rams of the Djallonké breed, weighing on average 20.0 kg, were used to evaluate the
voluntary intake and digestibility of hay of A. gayanus, foliage of A. africana (as a sole feed), and A. africana, P. erinaceus and K. senegalensis offered with 30% of the diet as A. gayanus hay. The crude protein (CP) content of A. africana, and P. erinaceus decreased significantly from the late dry season to the cool season when that of K. senegalensis tended to increase. The mean CP of A. africana, P. erinaceus and K. senegalensis differed significantly (173 g, 139 g and 114 g/kg DM, respectively). The DM intake of A. africana offered with hay (571 g/d) or as a sole feed (598 g/d) were not significantly different, but was higher than that of P. erinaceus (428 g/d) and K. senegalensis (298 g/d). The digestibility calculated by difference of DM and CP of A. africana (582 g/kg DM and 795 g/kg CP, respectively) did not differ significantly from A. africana as a sole feed, but were higher than for the other species. The nutritive value of A. africana seems to justify the high preference of herders for this species. 相似文献
75.
S. Kennou Sebei R. Bergaoui M. Ben Hamouda R. G. Cooper 《Tropical animal health and production》2009,41(7):1427-1438
The aim of the current investigation was to determine wild ostrich reproductive behaviour in Orbata Nature Reserve by observing
16 hens and 28 cocks over a seven-year period. Intense laying commenced in January, one month after the cessation of the rainy
season, and 92% of the eggs were produced during the dry season (January to May, peaking in March). Over the seven years,
1,322 eggs were laid in 69 nests, which corresponded to an annual average production of 19.2 ± 9.1 eggs/nest and 11.8 eggs/hen.
24 nests (34.78%) were non-brooded, 17 nests (24.64%) were deserted in the course of incubation, and 28 nests (40.58%) possessed
hatched eggs. All the non-incubated nests had egg losses equivalent to 46.6 ± 12.6%. Hatchability success of incubated eggs
was 41.9 ± 12.0%. Ostriches tended to dig their nests adjacent to the reserve enclosure which had direct access by road and
track, the latter subjecting them to human disturbance and predation. The systematic obstruction of these nests stimulated
ostriches to build additional nests within the reserve perimeter. The authors discussed the results recorded in an ostrich
flock in relation to the environmental factors (climatic factors, food disponibility and predation) and suggested possibilities
for improved wildlife management. 相似文献
76.
Effects of tea saponins on rumen microbiota,rumen fermentation,methane production and growth performance—a review 总被引:1,自引:0,他引:1
Reducing methane emission from ruminant animals has implications not only for global environmental protection but also for
efficient animal production. Tea saponins (TS) extracted from seeds, leaves or roots of tea plant are pentacyclic triterpenes.
They have a lasting antiprotozoal effect, but little effect on the methanogen population in sheep. There was no significant
correlation between the protozoa counts and methanogens. The TS decreased methanogen activity. It seems that TS influenced
the activity of the methanogens indirectly via the depressed ciliate protozoal population. The TS addition decreased fungal
population in the medium containing rumen liquor in in vitro fermentation, but no such effect was observed in the rumen liquor
of sheep fed TS. Tea saponins had a minor effect on the pattern of rumen fermentation and hence on nutrient digestion. When
added at 3 g/day in diets, TS could improve daily weight gain and feed efficiency in goats. No positive associative effect
existed between TS and disodium fumarate or soybean oil on methane suppression. Inclusion of TS in diets may be an effective
way for improving feed efficiency in ruminants. 相似文献
77.
Frankena K Somers JG Schouten WG van Stek JV Metz JH Stassen EN Graat EA 《Preventive veterinary medicine》2009,88(2):150-157
This study describes the effects of floor system, digital dermatitis (DD) and interdigital dermatitis and heel-horn erosion (IDHE) on locomotion performance in 225 dairy cows of 12 commercial dairy herds. Nine herds were kept in cubicle houses with concrete passageways (either solid, slatted, or grooved concrete) and three herds were kept in straw yards. Animals were at most five times examined at monthly intervals for lesion severity of DD and IDHE and for locomotion score. Locomotion score was rated on a scale ranging from 1 to 5 (from normal to severe) and disturbed locomotion (lameness) was defined as a score > or =3. A logistic regression model was used to analyze the 943 observations using lameness (yes/no) as outcome variable. The proportion of observations scored as lame (locomotion score > or =3) increased from 18% 1 month after trimming to 29% at 4 months after trimming. Severe lesions of DD and IDHE were associated with a significantly higher proportion of lame cows. The proportion of animals with disturbed locomotion increased from 16% to 40% as the severity of DD increased and from 17% to 30% with increasing severity of IDHE lesions. Locomotion performance highly differed between the cubicle house and straw yard group. Only 1% of all gaits in straw yard cows were scored as lame, while in cubicle housed cows these percentages varied from 24% to 46% with grooved floors showing the highest average locomotion score. Due to the extreme low incidence of lameness in straw yards, the statistical analysis had to be restricted to observations on concrete floors (n=744). The logistic regression model with lameness (yes/no) as dependent variable and random effects of cow and herd resulted in Odds Ratios for severe DD and IDHE of, respectively, 3.2 and 3.2, both significantly larger than unity. Cows housed at grooved concrete floors showed the highest OR of 6.5 compared to solid concrete floors. Recovery of lameness was poor as disturbance in gait lasted several months. 相似文献
78.
Millogo V Norell L Ouédraogo GA Svennersten-Sjaunja K Agenäs S 《Tropical animal health and production》2012,44(5):1017-1025
The aim of the study was to test three different hand-milking techniques (“pull down”, “thumb in”, and “full hand grip”) and
their effects on milk production and teat treatment. This is important since milk production in many tropical areas still
rely on hand-milking. The study was carried out at a peri-urban farm in the Bobo-Dioulasso area in Burkina Faso. Twelve indigenous
Zebu cows in early lactation were used in the study. The sequences of the treatments (hand-milking techniques) and the milkers
were balanced for carry-over effects between successive periods and days, respectively. The sequences were constructed by
using special Latin squares. Yield and composition of saleable milk was not affected by milking technique but there were differences
between the milkers in milk yield. There was also a significant interaction for saleable milk yield between milker and milking
technique. Hemoglobin in milk was measured as an indicator of teat damage. The hemoglobin content was numerically higher in
post-milking strip milk samples than in saleable milk. It was concluded that the amount of milk removed depends mainly on
the milker and how well the milking technique works for the individual milker. No effect of milking technique was observed
on teat treatment. 相似文献
79.
Adrian Muwonge Clovice Kankya Jacques Godfroid Berit Djonne John Opuda-Asibo Demelash Biffa Takele Ayanaw Musso Munyeme Eystein Skjerve 《Tropical animal health and production》2010,42(5):905-913
To date, the public health relevance of mycobacterial infections in pigs is not well investigated despite high risk of infection.
Recently, there has been a documented increase in opportunistic infections and risk of acquiring opportunistic mycobacterial
infections in HIV/AIDS patients in Mubende district; unfortunately, there has been no published information on the epidemiology
of mycobacterial infections in this area. This study was carried out between September 2008 and February 2009. Investigations
were done to assess the prevalence and associated risk factors of mycobacterial infections in slaughtered pigs in Mubende
district of Uganda. A total of 997 pigs (53.7% male and 46.3% female) from 31 different slaughterhouses were examined for
the presence of lesions compatible with TB and mycobacterial infections. Pathologic tissue specimens were collected for culturing
and isolation of mycobacteria. A cross-sectional technique was used based on convenient visits to slaughterhouses but random
selection of individual slaughtered pigs for a detailed post-mortem inspection on a daily basis. The results reflected a 9.3%
and 3.1% (95% CI) prevalence of Mycobacterium species based on necropsy examinations and culture isolation, respectively. The highest prevalence of mycobacterial infection
was recorded in Buwekula County (the mixed agro-zone) whilst the lowest was in Kassanda County (pastoral zone). A multivariable
logistical regression analysis identified age (P ≤ 0.001) and sex (P ≤ 0.05) as risk factors for mycobacterial infections in pigs. Post-estimation statistics of the regression model evaluation
and validation fit it well into the data (HL, χ
2 = 5.9; P = 0.69 for necropsy, HL χ
2 = 2.9; P = 0.94 for culturing). This study documented a high prevalence of mycobacterial infections in slaughter pigs in Mubende district.
The fact that pigs and human often share common housing and environment poses a high risk of zoonotic transmission. This then
warrants further molecular investigation to identify the specific Mycobacterium species and their public health importance in this area. 相似文献
80.