落叶松优良无性系苗木繁育技术

以苇河林业局东风林场为实验基地,结合固定标准地和临时标准地调查,从落叶松优良无性系苗木繁育方面进行研究,探讨落叶松无性繁育技术,为速生丰产林提供优良无性系苗木。     

荒漠草原牧草贮藏养分积累与消耗规律的研究

１９９４年在内蒙古达茂联合旗荒漠草原上，对１０种牧草的贮藏养分积累与消耗规律进行了研究。采用３．５一二硝基水杨酸比色法对野外采集的样品进行贮藏碳水化合物的定量分析。试验结果表明，禾本科植物贮藏碳水化合物含量的季节变化形成双峰或多峰曲线，双子叶植物则一般为扩展的Ｕ型曲线。作者对试验结果和出现的异常情况进行了分析和讨论。     

胡杨悬浮细胞的生长及液泡膜微囊的制备

改进了胡杨细胞悬浮培养体系，细胞得率提高了3倍多。做出了胡杨悬浮细胞：的生长曲线，以培养12～15d为宜。摸索了胡杨细胞破壁技术，使用了研磨、超声、捣碎、French Press法。从蛋白产率、v．H^ ATPaSe活力、反应潜势三个方面考虑选用捣碎法，结合差速离心和蔗糖密度梯度分离纯化液泡膜微囊，完善了从胡杨细胞分离纯化液泡膜微囊的试验流程，从而使液泡膜H^ ATPase的研究成为可能。     

水生光合生物无机碳的运输

本文综述了水生光合生物无机碳的运输。作为光合作用的无机碳源ＣＯ２和ＨＣＯ３－是水生光合生物最好的运输形式。但是，不同类型的水生光合生物无机碳的运输形式及其运输机理各不相同。     

Current Status and Enlightenment of Production and Trade of Forage Product in China北大核心CSCD

At present,China's traditional agriculture and animal husbandry has no way to rely on the "fine fodder + straw" feeding model. Only relying on grass and animal husbandry can guarantee China's consumer demand and food safety. However, due to the restraints such as policy, infrastructure, technology, market and organizational factors, the "lacking" status of China's pasture industry is not optimistic. In this paper, we analyze the pasture production, commercial grass production, grass seed production,forage product trade, the import and export volume of different types of pasture products, the current status of pastoral products trading partners and the causes behind it in depth. And concluded that the supply of forage products in China needs to be imported. Imports are growing while exports are generally declining,the pasture trade market is highly concentrated while grassland trading partners are dispersed. Five suggestions are given to provide theoretical guidance for the development of China's pasture industry: increase policy support, promote infrastructure, increase investment in scientific research, strengthen market construction, and develop industrial organizations. © 2019 China Agricultural University. All rights reserved.     

呼伦贝尔典型草原的利用方式对地上啮齿动物群落多样性的影响

2012年7、9月及2013-2014年5、7、9月,采用标志重捕法研究了连续放牧、季节轮牧和禁牧对呼伦贝尔典型草原啮齿动物捕获率、捕获量比例、多样性指数(丰富度指数、均匀性指数、Shannon-Wiener指数)的影响。结果表明,1)啮齿动物捕获率表现为连续放牧季节轮牧禁牧,连续放牧区显著高于禁牧区(P0.05)。2)不同放牧方式使啮齿动物群落结构发生了一定的变化。连续放牧区主要以草原黄鼠(Citellus dauricus)为主,捕获量比例为38.60%;季节轮牧区以黑线仓鼠(Cricetulus barabensis)和草原黄鼠占的比例较大,捕获量比例分别为37.30%和31.50%;禁牧区以黑线仓鼠为主,捕获量比例为38.89%。3)连续放牧区啮齿动物丰富度指数、Shannon-Wiener多样性指数均为最高;季节轮牧中均匀性指数为最高;禁牧中丰富度指数、Shannon-Wiener指数、均匀性指数均为最低。连续放牧中的丰富度指数和Shannon-Wiener指数显著高于禁牧(P0.05)。不同放牧方式明显改变了啮齿动物群落组成及多样性。综上,本研究结果表明,季节轮牧方式更利于合理利用草地资源并且能够抑制鼠害发生。     

Effect of dietary vanadium and vitamin C on egg quality and antioxidant status in laying hens

This study assessed the effect of dietary vanadium (V) and vitamin C (VC) on production performance, egg quality and antioxidant status in laying hens. A total of 360 laying hens (31‐week‐old) were randomly allotted into a 3 × 3 factorial arrangement treatments (four replicates and 10 chicks per replicate) with three levels of dietary V (0, 5 and 10 mg/kg) and three levels of vitamin C (0, 50 and 100 mg/kg) for 12 weeks. The effect of V and VC did not alter egg production, egg weight, average daily feed intake and feed conversion ratio during 1–12 week. Albumen height and Haugh unit value were linearly decreased (p < 0.001) by addition of V, whereas the effect of 100 mg/kg VC was observed to counteract (p < 0.05) this effect in V‐containing treatments during 1–12 week. Hens fed V‐containing diet laid lighter (linear effect, p < 0.05) coloured eggs (higher lightness value, lower redness and yellowness value), and the VC exerted no influence on it during 1–12 week. The serum superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH‐Px) activities, ability to inhibit hydroxyl radical, were significantly decreased, and the malondialdehyde (MDA) and V contents were increased (p < 0.05) by effect of V during 4, 8 and 12 week. The effect of VC alone and the interactive effect between VC and V were shown to increase serum (p < 0.05) SOD activity in 4 week and decrease MAD levels in 12 week. The result indicate that V decreased the egg quality and caused the oxidative stress at level of 5 mg/kg and 10 mg/kg, and the addition of 100 mg/kg vitamin C can alleviate its egg quality reduction effect and can mitigate the oxidative stress to some extent.     

肉鸡Trx1和Trx2蛋白的表达纯化及其抗氧化应激效果评价

试验旨在对肉鸡Trx1和Trx2蛋白进行表达和纯化,并评价其抗氧化特性。将原核表达载体GgTrx1-pET28a(+)和GgTrx2-nsp-pET28a(+)转入大肠埃希菌中进行蛋白表达;应用镍柱亲和层析的方法对该融合蛋白进行纯化;采用胰岛素还原法对重组肉鸡Trx1和Trx2蛋白(GgTrx1和GgTrx2)进行活性鉴定;通过细胞体外试验分析比较GgTrx1和GgTrx2对大鼠肝细胞BRL-3A氧化应激保护作用的影响。结果显示,试验成功获得两种重组蛋白:GgTrx1和GgTrx2,最适诱导条件分别为37℃、0.4mmol/L IPTG诱导4h和37℃、1.0mmol/L IPTG诱导4h;纯化的重组蛋白GgTrx1和GgTrx2纯度可达到90%,浓度分别达到4.0和5.0 mg/mL。重组蛋白GgTrx1和GgTrx2均具有较高的还原胰岛素二硫键的能力,并呈现出浓度效应。体外细胞试验发现,重组蛋白GgTrx1和GgTrx2均能显著降低过氧化氢诱导的BRL-3A膜脂过氧化,保护抗氧化酶SOD和CAT活性,且重组蛋白GgTrx2效果更明显。本试验结果表明,重组蛋白GgTrx1和GgTrx2均具有生物学活性和良好的抗氧化应激特性,且线粒体型Trx2为临床治疗氧化应激性疾病提供了更多可能。