益母草对绿壳蛋鸡血液生化指标及激素的影响

将280只50周龄健康、生产性能相近的黑羽绿壳蛋鸡随机均分为4组,每组设5个重复,每个重复14只。对照组饲喂基础日粮,试验组在基础日粮中分别添加0.5%、1.0%、1.5%益母草粉,观察其对黑羽绿壳蛋鸡血清生化指标、血液激素及卵巢的影响。结果表明,与对照组相比,在绿壳蛋鸡日粮中添加1.0%和1.5%益母草粉可显著降低血清中总胆固醇(TC)、甘油三酯(TG)和低密度脂蛋白(LDL)含量,提高高密度脂蛋白(HDL)、雌二醇(E₂)和促卵泡激素(FSH)含量,增加卵巢相对重量。益母草可以影响脂肪代谢,具有降低血脂的作用,考虑到生产成本问题,最适添加量为1.0%。     

农杆菌介导的xynB基因转化苜蓿的初步研究

构建了PBI121-xynB表达载体,利用农杆菌介导法进行紫花苜蓿遗传转化研究,将xynB基因的cDNA序列导入苜蓿。转基因植株生长发育良好,RT—PCR检测xynB基因已经导入到苜蓿中,DNS方法检测转基因植株的木聚糖酶活性为10.5 U/g鲜叶片。     

原料表面碱液处理对稻草碎料板力学性能的影响

在实验室条件下探索了稻草原料表面碱液处理对稻草碎料板力学性能的影响.试验表明,处理后的稻草碎料板的内结合强度比处理前有大幅度提高,弯曲弹性模量和静曲强度比处理前则有所下降,但都达到了相关的国家标准;2h吸水厚度膨胀率升高,超过国家标准.因此稻草表面碱液处理提高了稻草原料表面润湿性和胶合强度,但对稻草碎料板的力学性能有影响.     

云南含笑地被苗培育技术

阐述了云南含笑的形态特征、生物学特性,从育苗技术、苗木移植、整形修剪等技术环节总结了云南含笑地被苗的培育技术。     

采用PCR-SSCP技术分析蚯蚓粪便的微生物群落结构

通过SDS/蛋白酶的方法抽提蚯蚓粪便微生物总DNA,以总DNA为模板,采用通用引物分别成功扩增细菌16S rRNA基因的V4-V5可变区,真菌18S rRNA基因的V8-V9可变区,应用单链构象多态性技术（SSCP）分析了蚯蚓粪便中不同空间层次的微生物种群的多样性。结果表明,在粪便的不同空间层次上呈现出明显的空间分布多样性,并且与纯蚯蚓粪微生物种群的多样性有很大的差别。为进一步的蚯蚓粪生态学功能研究提供了有益的指导。     

Association of toll‐like receptor 2 polymorphisms with somatic cell score in Xinjiang Brown cattle

This study aims to identify single nucleotide polymorphisms (SNPs) and haplotypes in the TLR2 gene, and analyze the association of SNPs or haplotypes and somatic cell scores in 151 Xinjiang Brown cattle and 138 Holsteins to evaluate the role of TLR2 during intramammary infections. TLR2 coding region was amplified by PCR and screened for SNP sequencing. Genotypes and frequencies of SNPs were identified. Finally, the associations of genotypes or haplotypes and somatic cell scores (SCS) were analyzed. The results showed that: (i) 15 SNPs (E+653, E+945, E+978, E+1010, E+1250, E+1688, E+1707, E+1779, E+1782, E+1891, E+1995, E+2025, E+2055, E+2214 and E+2295) were observed and detected from 289 cows; (ii) distribution of the 14 SNPs were significantly different from Xinjiang Brown cattle and Holstein (P < 0.001) except for the E+945 (P > 0.05); (iii) in 11 SNPs (E+945, E+978, E+1010, E+1688, E+1707, E+1779, E+1782, E+1995, E+2025, E+2055 and E+2214), the SCS of AB genotype was lower than AA (P < 0.05) in Xinjiang Brown cattle; and (iv) haplotypes composed of the above‐mentioned 11 SNPs were constructed. The SCS of cattle with Hap5 was lower than that of Hap3 (P < 0.05). This suggests that Hap5 might play an important role in sub‐mastitis resistance in Xinjiang Brown cattle.     

梅花鹿生长激素基因的克隆及序列分析

对梅花鹿的生长激素基因I部分片段进行了克隆测序 ,序列长度为 14 6 6bp ,现已将该序列提交到GeneBank上。GeneBank中的Blast分析表明 ,梅花鹿生长激素基因I与马鹿同源性为 99% ,与鼷鹿同源性为84 %。     

珍珠粟在畜禽饲料中的应用研究进展

珍珠粟作为一种重要的热带谷类作物而广泛地种植于半干旱地区,坑旱、耐瘠,其籽实蛋白质含量高,氨基酸组成合理,含抗营养因子少,作为玉米的替代品而广泛地应用于畜禽养殖业中,尤其在非反刍动物饲料中的应用效果较好。     

雌雄蛹自动分选机的研制

雌雄蚕蛹分选是制造优质蚕种的重要环节。依据雌雄蛹体重量和体形大小存在明显差异的特点 ,研制成功三段式雌雄分选机。经生产测试 ,每小时可分选 135 96kg茧的蚕蛹 ,分选雌雄蛹的误鉴率平均为 3 18% ,比人工分选的准确率高 ;通过调节分选孔的大小 ,准确率可达 99 5 %以上 ;在复眼着黑后分选对蛹体无损伤。机械操作简易 ,符合生产要求     

Endozoochorous seed dispersal by golden snub-nosed monkeys in a temperate forest

Seed dispersal is essential for plant recruitment and the maintenance of biodiversity. Colobine monkeys are primarily folivorous, but they also consume fruits and are often assumed to be seed predators. Although they are known to be epizoochorous seed dispersers, their role as endozoochorous seed dispersers needs reassessment. We examined potential endozoochory in golden snub-nosed monkeys (Rhinopithecus roxellana) at Dalongtan in Shennongjia National Park, central China, by assessing potential germination of ingested seeds (n = 1806, 9 species) from fecal samples. Intact seeds were in almost all fecal samples (ranging from 5–130 seeds), and ingested seeds were from small seeded species (seed width <4.5 mm). The 2 most abundant species were Actinidia arguta (73%) and Rosa caudata (15%). The fruits of A. arguta were unripe when ingested (i.e. effective seed predation) and the ingested seeds did not germinate in the trials. Therefore, ingestion of unripe seeds does not lead to effective seed dispersal. However, germination rates of defecated R. caudata (9%) were greater than control seeds (6% and 0%), demonstrating potential endozoochorous seed dispersal. Thus, colobine monkeys do indeed disperse mainly small-seeded from multi-seeded fruits through potential endozoochory and this process enhances the recruitment of seedlings.