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991.
A fungus, Fusarium solani, isolated from the soil, degraded ioxynil (3,5-diiodo-4-hydroxyben-zonitrile) in pure culture into at least eight products. Five products were detected in the organic fractions extracted from a culture grown in [14C]cyano-labeled ioxynil. Three additional products were separated by ion-exchange chromatography of the acidified aqueous phase. Cultures grown in the presence of [14C]ring-labeled ioxynil produced the same products in the organic extract and four to five products in the aqueous phase. The cyano-carbon of ioxynil was released as CO2 at a faster rate than that of ring-carbons and was released after the initial ring cleavage. Two of the metabolites were identified as 3,5-diiodo-4-hydroxybenzamide and 3,5-diiodo-4-hydroxybenzoic acid. 相似文献
992.
993.
A method was devised for the extraction and measurement of adenosine 5'-triphosphate (ATP) in soil that minimizes sorption of ATP on the soil colloids. Soil was ultrasonified for 1 min with a solution containing trichloracetic acid (0.5 m). disodium hydrogen orthophosphate (0.25 m) and paraquat dichloride (0.1 m). The ATP content of the filtered extract was determined without further treatment in a scintillation spectrometer by the firefly luciferin-luciferase system. Recovery of added ATP was greater using the extratant containing trichloracetic acid, orthophosphate and paraquat than with trichloracetic acid alone or with a sulphuric acid extradant. Recoveries of added ATP ranged from 45% to 84% in thirteen different soils; ATP contents from 0.64 to 9.03 μg g?1 soil. 相似文献
994.
Production of surface casts and the removal of plant litter from the soil surface by earth-worms had similar seasonal variations, with maximum values in May and minimum values occurring in July and August. Seasonal variations in the total nitrogen (TN) and oxidizable carbon contents of casts were closely related to variations in litter production. The C:N ratio of casts (10.7) was consistently smaller than that of underlying soil material (15.0 and 14.2 for the 0–5 and 18–22 cm depths, respectively), which is probably due to the mineralization of plant-derived organic material during passage through earthworms and utilization of low C:N ratio litter. Seasonal changes in the amounts of inorganic N forms in casts showed a build-up of NH4+N in the cooler winter months (July and August), attaining a maximum of 112 μg.g?1. with a decrease in autumn (April and May) and early spring (September and October), reaching a minimum of 54 μg.g?1. The opposite trend existed for seasonal variations in the NO3?-N content of the casts. Because only minor fluctuations in the amounts of N forms were obtained for underlying soil material during the casting period, the more dramatic changes observed in the casts could not be explained by soil variations. Seasonal variations in urease enzyme activity, associated with fluctuations in organic matter content, were more important than the effect of temperature on enzyme activity in accounting for seasonal variations in the NH4+-N content of casts. It was calculated that 73% of the TN content of litter removed from the surface by earthworms was accumulated in casts, indicating both the importance of earthworms in incorporating litter N with soil material and the inefficiency of N digestion by earthworms. 相似文献
995.
B.J. Macauley 《Soil biology & biochemistry》1979,11(2):175-179
Fungal succession on untreated and fungicide- and insecticide-treated leaves of Eucalyptus pauciflora was compared. Natural succession on untreated leaves fits the general scheme for succession on deciduous tree leaves. Initial colonizers are species of Coelomycetes and Moniliales. When the numbers of Coelomycetes decrease in the late stages of decomposition, the frequency of Penicillium species and species of Zygomycetes increases. The succession is affected only marginally by DDT and dieldrin treatment while benomyl severely restricts the occurrence of most fungal species. The effect of benomyl persists even after exposure for 48 weeks in the field. 相似文献
996.
The production of nitrous oxide by soils was studied over short periods at a range of moisture contents up to field capacity with a highly-sensitive gas Chromatographic method.Nitrous oxide (N2O) was emitted from all soils studied at all soil moisture contents, which ranged from air dry to field capacity. The rate of emission increased with increasing moisture content and with increasing temperature up to 37°C.The evolution of N2O was not due to displacement of soil air during wetting. It was inhibited by HgCl2 and toluene, and was prevented by formaldehyde and autoclaving. Thus it appeared to be due to microbiological processes.The results of experiments with nitrification and denitrification inhibitors suggest that a considerable part of the N2O was produced by the oxidation of ammonia. Production by denitrification of nitrate cannot be ruled out. The relative importance of these two mechanisms probably depends on the moisture and oxygen content of the soil.It is concluded that the microbial production of N2O is continuous in soil at all moisture contents. The process at low moisture contents constitutes an important component in the cycle which maintains the N2O concentration in the atmosphere. 相似文献
997.
B.E. Söderström 《Soil biology & biochemistry》1979,11(2):147-148
A technique for assessing the metabolically-active fungal biomass by means of fluorescein diacetate (FDA) staining was recently described (Söderström, 1977). In this note some practical details in the application of this technique to studies of FDA-active fungal biomass in the soil are discussed. 相似文献
998.
999.
Kermit Cromack Phillip Sollins William C. Graustein Karen Speidel Allen W. Todd Gody Spycher Ching Y. Li Robert L. Todd 《Soil biology & biochemistry》1979,11(5):463-468
Fungal mats of Hysterangium crassum (Tul. and Tul.) Fischer occupied a mean of 9.6% of the upper 10 cm of soil developed under a 40–65 yr old stand of Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) in Oregon. This hypogeous basidiomycete exudes large amounts of oxalic acid, some of which precipitates with Ca in microscopic crystals of calcium oxalate, resulting in a mean CaC2O4 content of 82g m?2 for the entire soil. Soil oxalate concentration was significantly greater within fungal mats (P < 0.01) and soil pH was significantly lower (P < 0.01) than in soil adjacent to mats. The quantity of Ca present as CaC2O4 is 0.5 the amount of exchangeable Ca in the soil and exceeds the mass of Ca lost annually in runoff. Scanning electron micrographs show intense chemical weathering, attributable to oxalate attack, in the immediate vicinity of hyphae. X-ray diffraction patterns of clay indicate bulk weathering is more intense within the fungal mats than in adjacent uncolonized soil. 相似文献
1000.
Interactions between eight ectomycorrhizal fungi and eight bacteria were tested on five laboratory media and in the rhizoplane of Pinus radiata. Depression of growth of the fungi by the bacteria in laboratory media was dependent on the medium and bore little relation to effects in the rhizoplane. In the rhizoplane, different bacteria could depress, have no effect or even stimulate growth of mycorrhizal fungi. Competition and antagonism are suggested as mechanisms for depression of the fungi. Some bacteria gave protection against the depressive effects of other bacteria. Considerable differences occurred between ectomycorrhizal fungi in their colonization of the rhizoplane in the absence of bacteria and also in their presence. The common mycorrhizal fungi Rhizopogon luteolus and Thelephora terrestris generally colonized roots well but the strain of Pisolithus tinctorius studied colonized poorly. Direct microscopy showed the percentage cover of the root by microorganisms was usually only 10–20%.It is proposed that interactions of ectomycorrhizal fungi with soil organisms are important in determining the successful introduction and persistence of inoculated ectomycorrhizal fungi. Fungi should be selected for compatibility with a wide range of soil microflora as well as efficiency in plant stimulation. 相似文献