Pathogenic and genetic variability in <Emphasis Type="Italic">Tilletia indica</Emphasis> monosporidial culture lines using universal rice primer-PCR

Tilletia indica Mitra is the causal agent of Karnal bunt of wheat, an important disease prevalent in several countries. The disease is internationally quarantined and the pathogen due to its heterothallic nature shows high variability. In the present study, we compared the pathogenic behaviour of various isolates of T. indica collected from different geographical locations of India and genetically characterized monosporidial (Ms) culture lines raised from these isolates of the pathogen. Pathogenic variability revealed existence of three pathotypes based on aggressiveness on a set of differential host genotypes. Monosporidial culture lines viz., 5 each from KB1, KB2, KB4 and KB5 and three lines of KB3 were established and analyzed genetically using 12 Universal Rice Primers (URPs). Amplification showed 98.44% polymorphism and primer URP 13R produced 100% polymorphic bands. Maximum similarity (83%) was between KB1MsB and KB1MsD as calculated by Jaccard’s similarity coefficient, whereas, minimum similarity was between KB1MsC and KB4MsB; KB1MsE and KB3MsA (46%). Three groups were formed among all Ms culture lines. One major group consisted of 13 lines with approximately 70% similarity, the second group consisted of 7 culture lines showing 55% similarity and the third group consisted of 3 Ms lines. URPs were able to differentiate the Ms culture lines raised from different T. indica isolates and the results indicated heterogeneity in the pathogen population.     

Genetic diversity in Acorus calamus L. as revealed by RAPD markers and its relationship with β-asarone content and ploidy level

β-Asarone content in Acorus calamus is a paramount issue because it limits the usage of plant for medicinal purpose. In the present study A. calamus L. accessions based on RAPD marker, ploidy level and β-asarone content were characterized and correlated on the basis of β-asarone content/ploidy level. Of the 40 random primers used, 6 primers generated polymorphism. Genetic relatedness among accessions evaluated by a similarity matrix based on Dice's coefficient ranged from 0.72 to 0.97. A phenetic dendrogram based on UPGMA analysis grouped accessions into two clusters. A. calamus L. accessions were found to be triploid and tetraploid and their β-asarone content was found in two ranges 6.92–8.0% and 73–88%. The study clustered the accessions as per their ploidy level, β-asarone content and geographical locations. This study would have extensive application in quality control of raw materials.     

The feeding ecology of elaterid larvae in central European arable land: New perspectives based on naturally occurring stable isotopes

To understand soil food webs, empirically generated data on the trophic connections and the feeding ecology of the major below-ground animal taxa are needed. Here we used stable isotope analysis to assess the trophic ecology of wireworms, the larvae of click beetles, in Central European arable land. Wireworms are amongst the major soil macroinvertebrates and are of practical importance in arable soils. Besides feeding on crops, they are thought to feed on weeds, soil organic matter (SOM), and even animal prey, but their feeding ecology is poorly studied under natural conditions. Elaterid larvae and their putative feeding substrates—plant roots, SOM, and litter—were sampled at 17 locations in Austria, Germany, and Italy and their isotope ratios of carbon (¹²C/¹³C) and nitrogen (¹⁴N/¹⁵N) measured to determine the wireworms’ trophic level, the importance of SOM and weeds within the diet of Agriotes larvae, as well as the individual diet variation in Agriotes obscurus larvae. δ¹⁵N signatures suggested that Agriotes larvae are predominately herbivorous, whereas the other wireworm species primarily fed on animal prey. In contrast to SOM, weeds were readily eaten by Agriotes larvae: their dietary contribution ranged between 28% and 67% in weedy maize fields. Most A. obscurus larvae fed on a mixed diet of weeds and maize, although ～15% of the larvae fed primarily on one of the two food sources only. δ¹⁵N signatures indicated that ～10% of the “herbivorous” A. obscurus larvae fed primarily on animal prey, revealing high intraspecific trophic plasticity in these soil insects. Wireworm feeding behaviour is apparently complex at the individual level: the population consists of types A and B generalists, a phenomenon which needs further assessment.     

Diversity of arbuscular mycorrhizal fungi associated with the rhizosphere of tea growing in ‘natural’ and ‘cultivated’ ecosites

The colonization and diversity of arbuscular mycorrhizal fungi (AMF) associated with the rhizosphere of tea [Camellia sinensis (L.) O. Kuntze] growing under ‘natural’ as well as ‘cultivated’ conditions in the Kumaun region of Uttaranchal Himalaya (India), during the periods of active growth and dormancy were investigated. Root and rhizosphere soil samples, collected from both the ecosites (natural and cultivated), were monitored for root colonization. While the percent root colonization was quite high (77.66 ± 4.40 and 86.40 ± 3.02%, in the natural and cultivated tea, respectively) during the period of active growth in both the ecosites, relatively higher colonization (97.33 ± 0.78 and 98.13 ± 0.80%, in the natural and cultivated tea, respectively) was recorded during the period of dormancy. The rhizosphere of cultivated tea bushes was found to be dominated by Glomus morhpotypes (88.89% of the total isolates) along with three morphotypes of Acaulospora; occurrence of 35 morphotypes belonging to four genera viz. Acaulospora (11.43%), Gigaspora (11.43%), Glomus (68.57%) and Scutellospora (8.57%) was recorded in the rhizosphere of tea plants from the natural ecosite. A total of 51 AMF morphotypes were detected. Shannon–Weaver index of diversity was higher (1.80 ± 0.13 and 2.05 ± 0.10 during periods of active growth and dormancy, respectively) at the species level for the natural ecosite over its counterparts from the cultivated ecosite. Values for the diversity indices of natural and cultivated ecosites did not show much variation in the period of dormancy. These data suggest that collectively, various cultural practices negatively affect AMF diversity at the genus level in tea plantations of the colder regions.     

Formation of furan and methylfuran by maillard-type reactions in model systems and food

The formation of furan and 2-methylfuran was studied in model systems based on sugars and selected amino acids. Both compounds were preferably formed under roasting conditions in closed systems yielding up to 330 micromol of furan and 260 micromol of 2-methylfuran per mol of precursor. The amounts obtained under pressure cooking conditions were much lower, usually below 20 micromol/mol, except for 2-furaldehyde, which yielded 70-100 micromol/mol of furan. Labeling studies indicated two major formation pathways for both furans: (i) from the intact sugar skeleton and (ii) by recombination of reactive C(2) and/or C(3) fragments. Under roasting conditions in the absence of amino acids, furan was mainly formed from the intact sugar skeleton. Formic and acetic acid were identified as byproducts of sugar degradation, indicating the split off of C(1) and/or C(2) units from hexoses. The presence of alanine, threonine, or serine promoted furan formation by the recombination of C(2) fragments, such as acetaldehyde and glycolaldehyde, which may originate from both sugars and amino acids. In aqueous solution, about half of furan was generated by the recombination of sugar fragments. 2-Methylfuran was preferably formed in the presence of amino acids by aldol-type reactions of C(2) and C(3) fragments with lactaldehyde as a key intermediate, the Strecker aldehyde of threonine. The total furan levels in cooked vegetables were increased by spiking with hexoses. However, in pumpkin puree, only about 20% of furan was formed from sugars, preferably from the intact carbon skeleton.     

Production of high yielding red raspberry long canes in a Northern climate

Techniques for the production of raspberry long canes with high yield potential were studied on a research station and in two commercial nurseries in South Norway. Potted ‘Glen Ample’ plants were grown in the open and in polyethylene greenhouses during two seasons in order to optimize cultural practices. Dates of propagation and transfer to the production sites were varied. Following cold storage from December to early June, the canes were tipped (cut) at 160 cm height and grown in open plastic tunnels in the fruiting season for the evaluation of growth and yield performance. Remarkable berry yields of 1.5–3.8 kg per plant (cane) were regularly achieved. When grown in the open, later starts than June 1 (June 15 and July 1) successively reduced yields. On the other hand, an earlier start involves risks of growth cessation due to low spring temperature. When grown in greenhouses, high yields were still possible when plants were transferred from propagation conditions as late as July 1, although optimal yields of nearly 4 kg per plant were obtained with transfer on June 15. In a year with unusually high summer temperatures, the yields of canes produced in the open and in greenhouses did not vary significantly, whereas in the second year with normal summer temperatures, canes produced in greenhouses consistently out-yielded those produced in the open by about 1 kg per cane. Regression analyses revealed that high yields were associated with fruiting cane architecture traits such as cane height, number and length of laterals, and a low proportion of dormant buds. The single most important component was lateral length which alone accounted for 82% of the yield variation. Since lateral lengths increased from the shoot tip towards the base, cane heights in excess of 2 m are required for the attainment of top yields as the uppermost nodes with short and low-yielding laterals then can be eliminated by tipping.     

Editorial

No abstract available for this article.     

Acremonium strictum pulmonary infection in a horse

A 10-year-old Thoroughbred gelding was admitted to the Veterinary Medical Teaching Hospital of the University of California-Davis with a 2-week history of intermittent fever and acute onset of lethargy, anorexia, and ataxia. Although the clinical signs were nonspecific, the results of initial hematologic and biochemical analysis were consistent with a chronic inflammatory process. Thoracic radiographs revealed an increased fine reticulonodular interstitial opacity throughout the dorsal caudal lung fields. Cytologic examination of bronchoalveolar lavage (BAL) fluid showed mixed inflammation with many mononuclear phagocytes containing single, spherical, intracytoplasmic fungal organisms. Four mold species were cultured in low numbers from the BAL fluid. One of the fungal elements observed on the culture plates was identified as Acremonium strictum by real-time polymerase chain reaction (PCR). A diagnosis of fungal pneumonia due to A strictum was made based on the results of thoracic imaging, cytologic evaluation, culture, and PCR testing. The horse made an uneventful recovery with supportive treatment and was disease-free based on normal physical, radiographic, and cytologic findings at 21 days after presentation. To our knowledge, this is the first report of isolation of A strictum from the BAL fluid of a horse with interstitial pneumonia.     

Environmental control of growth and flowering of Rubus idaeus L. cv. Glen Ample

Environmental control of the annual growth cycle of ‘Glen Ample’ raspberry has been studied in order to facilitate crop manipulation for out-of-season production. Plants propagated from root buds were raised in long days (LD) at 21 °C and then exposed to different temperature and daylength conditions at varying ages. Shoot growth was monitored by weekly measurements and floral initiation by regular sampling and examination of axillary bud #5. Under natural summer daylight conditions at 60°N shoot growth was nearly doubled at 21 °C compared with 15 °C, while at 9 °C one half of the plants ceased growing and formed flower buds at midsummer. Developing shoots have a juvenile phase and could not be induced to flower before the 15-leaf stage. No significant reduction in induction requirements was found in larger plants. Plants exposed to natural light conditions from 10th August, had an immediate growth suppression at 9 and 12 °C with complete cessation after 4 weeks (by September 7). This coincided with the first appearance of floral primordia. At 15 °C both growth cessation and floral initiation occurred 2 weeks later (by September 21), while at 18 °C continuous growth with no floral initiation was maintained until early November when the photoperiod had fallen below 9 h. The critical photoperiod for growth cessation and floral initiation at 15 °C was 15 h. Plants exposed to 10-h photoperiods at 9 °C for 2–4 weeks had a transient growth suppression followed by resumed growth under subsequent high temperature and LD conditions, while exposure for 5 or 6 weeks resulted in complete growth cessation and dormancy induction. The critical induction period for floral initiation was 3 weeks although no transitional changes were visible in the bud before week 4. When exposed to inductive conditions for marginal periods of 3 or 4 weeks, an increasing proportion of the plants (20% and 67%, respectively), behaved as primocane flowering cultivars with recurrent growth and terminal flowering. It is concluded that growth cessation and floral initiation in raspberry are jointly controlled by low temperature and short day conditions and coincide in time as parallel outputs from the same internal induction mechanism.     

Small urban woodlands as biodiversity conservation hot-spot: a multi-taxon approach

To evaluate the importance of urban woodlands to serve as potential sites for biodiversity conservation, we analysed bird, carabid beetle and small mammal community responses to urbanisation at different spatial scales. We analysed the relationships between the variations of the structure (species richness S, diversity H′ and dominance D) of animal communities of woodlands distributed along a rural–urban gradient, and the variations along this same gradient of (1) the vegetation within woodlands, (2) the landscape at 100 m and (3) 600 m around the woodlands. We identified the spatial scales whose variations along the gradient most affected each animal community structure, and characterised community responses to these variations. Our results showed that urbanisation affected taxa differently according to their dispersal ability. Carabid beetles, less mobile, seem to be sensitive to increasing fragmentation and built surfaces from periurban to town centre which could make their movement within the urban landscape difficult. Birds, mobile species, seem to be more sensitive to variations of the vegetation structure within woodlands from periurban to town centre that could affect their capacity to maintain in habitat patches. Although our study did not allow relating the small mammal community structure to urbanisation, it suggests that this taxa is sensitive to urban local disturbances. A relevant management scale of woodlands can be specified for each taxa conservation. Urban woodlands accommodate over 50% of the species present in periurban woodlands, and effective management could enhance this number. Woodlands seem to be a good choice for promoting biodiversity conservation in towns.