云南生物多样性的现状及发展前景

对我国云南的动物资源、植物资源,生态系统类型,物种遗传资源等进行的分析研究,论述了云南生物多样性丰富性和特有性等组成特征,并分析了云南生物多样性现在存在的问题,提出相应的保护对策,并且提出了关于云南生物多样性的一点展望。     

宁洱县巨龙竹引种试验林竹丛结构现状分析与经营管理

巨龙竹(Dendrocalamus sinicus)是云南特有的大型合轴丛生竹类,是迄今为止发现的世界上最大的竹种。其秆高可达30m以上,径粗可达30cm以上,是我国南部热区及世界热带至南亚热带地区推广前景最为广阔的优良经济竹种之一。分析论述了巨龙竹在宁洱县两个引种地的竹丛结构和生长状况,根据巨龙竹的秆高与胸径的关系和新竹与老竹的生长状况的关系来和原产地的巨龙竹生长状况做出对比,找出了其在引种地的生长规律,提出了与之相适应的经营管理措施,以实现巨龙竹的高产与稳产。     

应用水垢指数法对贵阳污水热能适用性的分析

为了解贵阳市污水热能的适用性,预测贵阳市污水对污水热能应用的热交换设备产生腐蚀和结垢情况,可为污水热能的利用提供理论数据支持。通过对贵阳市城市污水水质的监测,分析出贵阳市小河污水处理厂在温度变化较大的月份的污水指标值。应用朗格利尔饱和指数(LSI)法、赖兹纳稳定指数(RSI)法、帕科拉兹结垢指数(PSI)法3种指数分析方法,判断出贵阳市的污水腐蚀、结垢趋势。结果表明,贵阳市污水的可利用性较强,可以满足污水源热泵系统对水质的要求。     

Puqienine E: An angiotensin converting enzyme inhibitory steroidal alkaloid from Fritillaria puqiensis

Eight steroidal alkaloids, puqienine A, puqienine B, puqienine C, puqienine D, puqienine E, puqietinone, puqiedine and peimisine were isolated from Fritillaria puqiensis G. D. Yu et. G. Y. Chen, and their anti-hypertensive effect were assessed in vitro based on the inhibition of the purified angiotensin converting enzyme (ACE) using high-performance liquid chromatography assay. The results showed that puqienine E, puqienine B and puqienine A exhibited better inhibitory activity against ACE than others, with inhibition ratios of 70.2 ± 0.5%, 24.7 ± 0.5% and 20.4 ± 2.8%, respectively at the concentration of 200 μM. The 50% inhibiting concentration of puqienine E was determined to be 68 µM.     

Genetic analysis of Akabane virus isolates from cattle in Korea

Bayesian Inference (BI) and Neighbor Joining (NJ) analyses of the phylogenetic relationships between the nucleotide sequences of the N gene of Akabane virus revealed an unclear topology among genogroups I–III, which was probably caused by genetic reassortment or recombination between these genogroups. In contrast, nucleotide and amino acid phylogenetic tree analyses of the M RNA segment agreed with the topologies obtained by using the BI and NJ methods. Therefore, distinct genogrouping of Akabane virus isolates should be performed using the M RNA segment. Four Korea isolates were classified into genogroup II together with Akabane virus strains isolated from all areas of Japan, including Okinawa Island. However, more nationwide isolates and more clinical data from Korean cattle farms will be required in the future to confirm the precise relationships between genotypes and pathogenicity.     

Crystal growth inhibitors for the prevention of L-cystine kidney stones through molecular design

Crystallization of L-cystine is a critical step in the pathogenesis of cystine kidney stones. Treatments for this disease are somewhat effective but often lead to adverse side effects. Real-time in situ atomic force microscopy (AFM) reveals that L-cystine dimethylester (L-CDME) and L-cystine methylester (L-CME) dramatically reduce the growth velocity of the six symmetry-equivalent {100} steps because of specific binding at the crystal surface, which frustrates the attachment of L-cystine molecules. L-CDME and L-CME produce l-cystine crystals with different habits that reveal distinct binding modes at the crystal surfaces. The AFM observations are mirrored by reduced crystal yield and crystal size in the presence of L-CDME and L-CME, collectively suggesting a new pathway to the prevention of L-cystine stones by rational design of crystal growth inhibitors.     

注重选人用人的公信度加强反腐倡廉建设

随着人事制度改革的不断深化,在人力资源管理方面构建惩治和预防腐败体系的重要性日趋显现。坚持正确的用人导向,把紧防治选人用人上的不正之风的关口;建立健全干部考核、管理制度,使反腐倡廉贯穿选人用人的全过程,对人事制度改革具有十分重要的特殊意义。     

大跨屋盖结构风效应的风洞试验与原型实测研究

以广州国际会展中心为工程案例,进行了刚性模型风洞试验和有限元模态分析,在此基础上计算了屋盖的风致位移响应,结果表明,对于屋盖风振响应影响最大的因素是结构的基阶振型,其次才是风荷载;随着阻尼比的增加,位移响应谱的共振峰得到了有效平抑,峰值位移响应也随之减小.基于现场实测,提出功率谱点积识别大跨屋盖结构竖向整体振动固有频率的方法,并与有限元计算结果进行了对比分析,实测得到的前4阶固有频率与振型均能与有限元模型较好吻合,其中基阶固有频率的相对误差仅为0.3%,证明了该方法的有效性.     

大豆C4H基因克隆及生物信息学分析

为了从分子水平上揭示大豆C4H的结构特点,并为提高其表达活性提供理论依据,利用RT-PCR技术克隆了大豆C4H基因,并已登录GenBank(登录号为FJ770468),长度为1 766 bp,编码区1 521 bp,编码一个含有506个氨基酸残基的多肽。生物信息学分析显示,C4H理论PI=5.30,Mw=39.092 ku;C4H是易溶、亲水性较强的蛋白,同时有两个明显的疏水峰,有2个跨膜肽段;通过HNN分析表明,C4H各个氨基酸残基对应的二级结构分别为α螺旋(Alpha helix)(Hh):251,49.60%,β折叠(Extended strand)(Ee):52,10.28%,无规卷曲(Random coil)(Cc):203,40.12%;Geno3d模建预测,C4H蛋白中β折叠区有57个折叠,折叠区间有24个α螺旋,此外还有14个卷曲结构。氨基酸序列和结构分析显示C4H蛋白包含了一个保守区,即P450 domain。利用SignalP分析得到信号肽存在几率、信号肽锚定几率均为0.498,切割位点位于28和29位氨基酸之间。