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51.
Hochi S Abdalla H Hara H Hirabayashi M 《The Journal of reproduction and development》2011,57(5):557-563
Freeze-drying (lyophilization) has been proposed as an alternative method for sperm preservation to overcome the disadvantages of the current cryopreservation method such as the high maintenance cost of frozen stocks, the problems associated with transportation of frozen materials and the potential risk of total loss of the frozen stock. Since freeze-dried spermatozoa after rehydration lose their motility, which is an essential requirement to complete physiological fertilization, a relatively difficult microinsemination technique must be applied to rehydrated spermatozoa. Theoretically, it has been supposed that freeze-dried spermatozoa could maintain their functions and abilities to interact with the oocyte cytoplasm after prolonged storage at refrigerator temperature. However, sufficient yield of transferable blastocysts and production of live offspring derived from freeze-dried sperm samples are still subjects to be challenged and overcome in large domestic species. 相似文献
52.
Terkawi MA Thekisoe OM Katsande C Latif AA Mans BJ Matthee O Mkize N Mabogoane N Marais F Yokoyama N Xuan X Igarashi I 《Veterinary parasitology》2011,182(2-4):337-342
A total of 719 serum samples collected from clinically healthy cattle from eight provinces located in different districts of South Africa were examined by the indirect enzyme-linked immunosorbent assay (ELISA) and the standard indirect fluorescent antibody test (IFAT) to determine the serological prevalence of Babesia bovis and Babesia bigemina. The results showed that 35.3% and 39.7% of cattle were positive for B. bovis and 30% and 36.5% were positive for B. bigemina antibodies on ELISA and IFAT, respectively. Mixed infections were detected in 18.2% and 26.3% of the samples using ELISA and IFAT, respectively. Consequently, the ELISAs with recombinant B. bovis spherical body protein-4 (BbSBP-4) and B. bigemina C-terminal rhoptry-associated protein-1 (BbigRAP-1/CT) were proven to be highly reliable in the serological diagnoses of bovine babesiosis in South African cattle, as evidenced by the significant concordance rates when the results were compared to those of IFAT. Moreover, the serological prevalence was significantly different among the tested provinces, in which the ranges exhibited between 15% and 73% for B. bovis infection and between 13% and 54% for B. bigemina infection. High sero-positive rates were present in Mpumalanga and KwaZulu-Natal provinces, while the lowest rate was in the North West province. Our data provide important information regarding the current seroprevalence of bovine babesiosis in South Africa, which might be beneficial in developing rational strategies for disease control and management. 相似文献
53.
Abdalla AL Louvandini H Sallam SM Bueno IC Tsai SM Figueira AV 《Tropical animal health and production》2012,44(5):953-964
The main objective of the present work was to study nutritive strategies for lessening the CH4 formation associated to ruminant tropical diets. In vitro gas production technique was used for evaluating the effect of
tannin-rich plants, essential oils, and biodiesel co-products on CH4 formation in three individual studies and a small chamber system to measure CH4 released by sheep for in vivo studies was developed. Microbial rumen population diversity from in vitro assays was studied
using qPCR. In vitro studies with tanniniferous plants, herbal plant essential oils derived from thyme, fennel, ginger, black
seed, and Eucalyptus oil (EuO) added to the basal diet and cakes of oleaginous plants (cotton, palm, castor plant, turnip,
and lupine), which were included in the basal diet to replace soybean meal, presented significant differences regarding fermentation
gas production and CH4 formation. In vivo assays were performed according to the results of the in vitro assays. Mimosa caesalpineaefolia, when supplemented to a basal diet (Tifton-85 hay Cynodon sp, corn grain, soybean meal, cotton seed meal, and mineral mixture) fed to adult Santa Ines sheep reduced enteric CH4 emission but the supplementation of the basal diet with EuO did not affect (P > 0.05) methane released. Regarding the microbial studies of rumen population diversity using qPCR with DNA samples collected
from the in vitro trials, the results showed shifts in microbial communities of the tannin-rich plants in relation to control
plant. This research demonstrated that tannin-rich M. caesepineapholia, essential oil from eucalyptus, and biodiesel co-products either in vitro or in vivo assays showed potential to mitigate
CH4 emission in ruminants. The microbial community study suggested that the reduction in CH4 production may be attributed to a decrease in fermentable substrate rather than to a direct effect on methanogenesis. 相似文献
54.
Seal A Kafwembe E Kassim IA Hong M Wesley A Wood J Abdalla F van den Briel T 《Public health nutrition》2008,11(7):720-728
OBJECTIVE: To assess changes in the Fe and vitamin A status of the population of Nangweshi refugee camp associated with the introduction of maize meal fortification. DESIGN: Pre- and post-intervention study using a longitudinal cohort. SETTING: Nangweshi refugee camp, Zambia. SUBJECTS: Two hundred and twelve adolescents (10-19 years), 157 children (6-59 months) and 118 women (20-49 years) were selected at random by household survey in July 2003 and followed up after 12 months. RESULTS: Maize grain was milled and fortified in two custom-designed mills installed at a central location in the camp and a daily ration of 400 g per person was distributed twice monthly to households as part of the routine food aid ration. During the intervention period mean Hb increased in children (0.87 g/dl; P < 0.001) and adolescents (0.24 g/dl; P = 0.043) but did not increase in women. Anaemia decreased in children by 23.4% (P < 0.001) but there was no significant change in adolescents or women. Serum transferrin receptor (log10-transformed) decreased by -0.082 microg/ml (P = 0.036) indicating an improvement in the Fe status of adolescents but there was no significant decrease in the prevalence of deficiency (-8.5%; P = 0.079). In adolescents, serum retinol increased by 0.16 micromol/l (P < 0.001) and vitamin A deficiency decreased by 26.1% (P < 0.001). CONCLUSIONS: The introduction of fortified maize meal led to a decrease in anaemia in children and a decrease in vitamin A deficiency in adolescents. Centralised, camp-level milling and fortification of maize meal is a feasible and pertinent intervention in food aid operations. 相似文献
55.
Studies on gangrenous mastitis in goats 总被引:1,自引:0,他引:1
M T Abu-Samra S M Elsanousi M A Abdalla A A Gameel M Abdel Aziz B Abbas K E Ibrahim S O Idris 《The Cornell veterinarian》1988,78(3):281-300
One hundred and fifty goats were diagnosed as having proven gangrenous mastitis. The disease was categorized into early, intermediate and late stages. Gangrenous mastitis in goats is typified by a sudden onset, dark hyperemia, and edema with progressive discoloration of the distal part of the udder. The disease affected lactating goats but not the dry ones. Coagulase positive Staphylococcus aureus was isolated from 60% of composite and half milk samples obtained from the diseased goats. The histopathological changes mainly comprised proliferation of connective tissue, thrombosis and necrosis involving a group of lobules. Treatment of the early and intermediate stages of the disease was successful through the administration of systemic and intramammary terramycin together with diuretics and topical antiseptic cream. The late stage of the disease was successfully treated only through surgery. 相似文献
56.
A H Fayed E B Abdalla R R Anderson K Spencer H D Johnson 《American journal of veterinary research》1989,50(1):151-153
A study was performed to assess the effect of xylazine HCl (0.1 mg/kg of body weight, IV) in heifers maintained at thermoneutrality (18 C, 42% humidity) or under heat stress (33 C, 63% humidity) conditions. Xylazine caused 50 and 70% decreases in serum insulin concentrations in the thermoneutral and heat-stressed heifers, respectively. Xylazine-induced hypoinsulinemia was associated with hyperglycemia. In the thermoneutral group, serum glucose concentrations increased from a basal concentration of 75 mg/dl to 150 mg/dl after 15 minutes. In the heat stress group, the serum glucose concentration increased from 65 mg/dl to 105 mg/dl. Hyperglycemia peaked at 2 hours and remained high for 6 hours after xylazine administration. Heat-stressed heifers took a longer time (107 minutes) to stand than did heifers under thermoneutral conditions (41 minutes). The time to regain sensation to pain was significantly prolonged in heat-stressed heifers. Xylazine had no effect on body temperature and respiration rate in heifers under the thermoneutral condition, whereas it markedly induced hyperthermia and suppressed respiration rate in the heat-stressed heifers. Furthermore, the pulse rate was slightly decreased in thermoneutral heifers and was markedly decreased in the heat-stressed heifers. 相似文献
57.
Optimizing the manufacturing conditions of veneer strand-flanged I-beams was continued in this study and focused on the strand
density and preparation method. Three levels of strand density were used, while the strands were prepared by either saw or
roll-press splitter. The main results indicated that: within the compaction ratios (1.4–2.3) investigated in this study, the
strand with lower density showed slight improvement in the dimensional stability and the bond strength between web and flange,
but not in bending properties of the I-beams. The strand preparation method was concluded to be dependent on species for akamatsu,
sugi, and bamboo strands; roll-press splitter-prepared strands tended to negatively affect dimensional stability and mechanical
properties of the I-beams. When using akamatsu or sugi strands, low density allowed the possibility of using lower resin application
rates between strands.
Part of this paper was presented at the 54th Annual Meeting of the Japan Wood Research Society in Sapporo, August 2004 相似文献
58.
Salem Mohamed Omar Abdalla Salem Tarek A. Yürüten Özdemir Keriman Sönmez Adem Yavuz Bilen Soner Güney Kerim 《Fish physiology and biochemistry》2021,47(4):1053-1062
Fish Physiology and Biochemistry - In the present study, antioxidant activity, immune responses, and growth performance of rainbow trout (Onchorhynchus mykiss) juveniles fed with diets supplemented... 相似文献
59.
Ana Alfaro-Fernández Mai Abdalla Ali Fadia Mobarak Abdelraheem Ebrahim Abd Elhameed Saeed María Isabel Font San Ambrosio 《European journal of plant pathology / European Foundation for Plant Pathology》2012,133(4):791-795
In January 2011, symptomatic chickpea and faba bean plants were observed in fields located in the Gezira state (Sudan). Faba bean plants showed yellowing and stunting, whereas chickpea plants presented yellowing, reddening and little leaves. The disease etiology was investigated using nested polymerase chain reaction (PCR) with phytoplasma-specific primers which amplify a fragment of the 16S rRNA gene. Sequencing and restriction fragment length polymorphism (RFLP) analyses revealed that the tested phytoplasmas belonged to the group 16SrII. Phylogenetic analyses of the 16S rRNA gene of the obtained sequences indicated that the chickpea and faba bean phytoplasmas from Sudan were more closely related to the phytoplasmas subgroup 16SrII-D. To our knowledge, this is the first report of phytoplasmas from the group 16SrII-D infecting chickpea in Sudan, and faba bean worldwide. 相似文献
60.
Maiy M. M. Abdallah Ibrahim A. Adam Tamadur M. Abdalla Sanaa A. Abdelaziz Mohamed E. Ahmed Imadeldin E. Aradaib 《Irish veterinary journal》2015,69(1):6