德国核桃‘No.120'幼胚胚轴与子叶体细胞胚胎发生及其植株再生

核桃 (Juglansregia)‘No . 12 0’品系授粉 7～ 8周的幼胚胚轴与子叶在含不同植物生长调节剂组合的诱导培养基上培养 4周后转移至无植物生长调节剂的继代培养基上培养 ,不同发育时期的体细胞胚直接形成于胚轴与子叶表面。子叶与胚轴分别在KT 2mg/L BA1mg/L IBA 0 .0 1mg/L与 2 ,4 D 2mg/L KT 1mg/L的组合的诱导下体细胞胚胎发生频率最高。体细胞胚经 3～ 5℃低温处理 2个月 ,31.6%的体细胞胚能发育成正常生长的植株     

荔枝果皮的褐变

荔枝果皮的褐变一直是商业上的一个主要采后问题。大多数人认为褐变是由于果实失水后多酚氧化酶(PPO)活性增加引起的,它导致了花青苷的迅速降解,使果变褐。然而,支持此理论的实验证据很少。最近,澳大利亚和以色列着重研究果皮液泡的PH值对花青苷颜色和稳定性的影响。本文综述了研究荔枝果皮褐变的方法,并针对目前流行的PPO引起花青苷降解的理论提出一些疑问。     

Fibroblast growth factor 21 in dairy cows:current knowledge and potential relevanceOA

Fibroblast growth factor 21(FGF21) has been identified as an important regulator of carbohydrate and lipid metabolism, which plays an important role for metabolic regulation, particularly under conditions of energy deprivation or stress conditions. Dairy cows are subjected to a negative energy balance and various kinds of stress particularly during the periparturient phase and during early lactation. It has been shown that the plasma concentration of FGF21 in dairy cows is dramatically increased...     

Unveiling how vitrification affects the porcine blastocyst: clues from a transcriptomic studyOA

Background: Currently, there is a high demand for efficient pig embryo cryopreservation procedures in the porcine industry as well as for genetic diversity preservation and research purposes. To date, vitrification(VIT) is the most efficient method for pig embryo cryopreservation. Despite a high number of embryos survives in vitro after vitrification/warming procedures, the in vivo embryo survival rates after embryo transfer are variable among laboratories. So far, most studies have focused on c...     

Insights into the proteomic profile of newly harvested corn and metagenomic analysis of the broiler intestinal microbiotaOA

Background: The use of newly harvested corn in feed causes wet droppings in broilers and increased feed cost which was termed as “new season grain problem”. The present study was conducted to evaluate the proteomic profile of newly harvested corn and the subsequent influence on intestinal microbiol community for broiler chickens.Methods: Newly harvested corn stored for either half a month(HM) or two months(TM) was used, and the pasting properties, total soluble sugars, and proteomic analysis tec...     

Starchy and fibrous feedstuffs differ in their in vitro digestibility and fermentation characteristics and differently modulate gut microbiota of swineOA

Background: Alternative feedstuffs may contribute to reducing feed costs of pig production. But these feedstuffs are typically rich in fiber and resistant starch(RS). Dietary fibers and RS are fermented in the gastrointestinal tract(GIT) and modulate the microbial community. Certain microbes in the GIT can promote host health, depending on the type of fermentation substrates available. In this study, six alternative feedstuffs(three starchy: Okinawan sweet potato, OSP; yam, and taro, and three f...     

Inhibiting nuclear factor erythroid 2 related factor 2-mediated autophagy in bovine mammary epithelial cells induces oxidative stress in response to exogenous fatty acidsOA

Background: In early lactation, bovine mammary epithelial cells undergo serious metabolic challenges and oxidative stress both of which could be alleviated by activation of autophagy. Nuclear factor erythroid 2 related factor 2(NFE2L2), a master regulator of cellular redox homeostasis, plays an important role in the regulation of autophagy and oxidative stress. Thus, the objective of this study was to investigate the role of NFE2L2-mediated autophagy on oxidative stress of bovine mammary epithel...     

Insects as alternative feed for ruminants:comparison of protein evaluation methodsOA

Background: The high dependence of intensive ruminant production on soybean meal and the environmental impact of this crop encourage the search for alternative protein-rich feeds. The use of insects seems promising, but the extent of their ruminal protein degradation is largely unknown. This parameter has major influence not only on N utilization efficiency but also on the environmental burden of ruminant farming. In addition, although assessing ruminal N degradation represents a key first step ...     

A review of the resistome within the digestive tract of livestockOA

Antimicrobials have been widely used to prevent and treat infectious diseases and promote growth in food-production animals. However, the occurrence of antimicrobial resistance poses a huge threat to public and animal health, especial y in less developed countries where food-producing animals often intermingle with humans. To limit the spread of antimicrobial resistance from food-production animals to humans and the environment, it is essential to have a comprehensive knowledge of the role of th...     

Effect of protease supplementation on apparent ileal crude protein and amino acid digestibility of over-processed soybean meals in broilersOA

Background: Nutritional value of proteins in feed ingredients can be negatively affected by hydrothermal processing, which causes large variation in the bioavailability of amino acids(AA) and negatively affects animal productive performance. Supplementation of exogenous proteases could increase the rate of digestion of damaged proteins, thereby increasing overall AA digestibility and bioavailability. The aim was to determine the effect of exogenous protease supplementation on the apparent ileal ...