转基因与野生唐鱼耐温限度及窒息点的比较研究

对转红色荧光蛋白基因唐鱼、野生型唐鱼Ⅰ和野生型唐鱼Ⅱ的耐温和耐氧能力进行比较研究。结果表明,在驯化温度为30 °C,升温速率为1 °C / h时,三种唐鱼的半致死高温分别为：38.53±0.08 °C、38.28±0.29 °C和38.55±0.09 °C。在驯化温度为25 °C,降温速率为2 °C / 24h时,三种唐鱼的半致死低温分别为：1.13±0.40 °C、1.26±0.10 °C和1.21±0.12 °C。恒温25 °C时,测定三种唐鱼的窒息点分别为：0.66±0.02 mg/L、0.74±0.21 mg/L和0.51±0.01 mg/L。差异显著性检验表明,三种唐鱼对温度和溶氧的耐受力无显著性差异。     

元江自然保护区珍稀濒危植物红花木莲群落学研究

对元江自然保护区内珍稀濒危植物红花木莲群落进行研究,调查了群落物种组成、外貌、演替等,分析了群落的物种多样性。结果表明：（1）群落垂直结构明显,可分为乔木层、灌木层、草本层、层间植物。共有维管束植物110种,隶属47科80属。（2）群落的物种多样性高,草本层的各项物种多样性指数均高于乔木层和灌木层。（3）群落未发展到稳定状态,演替过程中优势种会有所变化。     

预油漆纸的应用及其生产工艺

本文介绍了预油漆纸的种类、基本性能及其在家具表面装饰和室内装修方面的应用，并且着重介绍了后浸型预油漆纸的主要原材料及生产工艺。     

不同板栗品种的组培技术

对檀桥、新田、韶十八、华光、红栗、铁粒头、安1、新田、焦扎、石丰、九家种等11个板栗品种的组织培养技术进行了研究.结果表明：最适培养基为WPM,MS;愈伤组织诱导成功的品种是檀桥、新田、韶十八、华光、红栗;外植体中胚的愈伤组织诱导率最高,野外材料以茎段的诱导率最高,为65%;不定芽的诱导中,檀桥生长最好;诱导生根中,华光可直接诱导出根.     

防沙治沙技术对策的探讨

土地沙化是我国特别是西北地区当前最为严重的生态环境问题，不断如剧的沙尘暴与沙化土地的扩张更使生态环境问题雪上加霜。文中根据中国沙化现状及世界防沙治沙经验展开思考，提出中国防沙治沙的总体方针和技术对策。     

小麦抗(耐)热种质资源的鉴定筛选

通过小麦抗 (耐 )热种质材料筛选方法的研究 ,计算冬春抗热总指数 ,确定抗热级别。从 2 0 0份鉴定材料中筛选出抗热级别为 1的 5个品种 (系 ) :97A - 5 ,96 - 171,M 2 4 ,4 47- 13- 2 ,97(6 ) 88,可作为优良抗 (耐 )热亲本材料供抗热育种使用。其中 97A - 5 ,M2 4等还可供大田生产使用。     

Technique of Classification of Coal and Gas Outburst Mines

managing the coal mines according their dangerous grade determined. can decrease the engineering quanity and lessen the sightless characteristic. It has marked benefit for community and great economic benefit. The dangerous grade of outburst mines are determined Outburst coal mines are classified into mines with serious outburst hazard, mines with middle outburst hazard and mines with ordinary outburst hazard. Twelve classification indicators are used in comprehensively evaluating the dangerous grade of outburst mines. The dangerous grade of outburst mines are determined according to the principle of the most subjection with fuzzy comprehensive evaluation method, on the basis of the classification indicators and its quantification. By the model founded, some coal mines are studied and the research results are accordant with the fact.     

Myeloperoxidase in Equine Semen: Concentration and Localization during Freezing Processing

Myeloperoxidase (MPO) is a pro-oxidant enzyme contained in and released by neutrophils during degranulation or after lysis. Post-thaw semen contains MPO, and concentration of this enzyme is associated with decreased motility. The aim of this study was to determine kinetics of MPO concentration during freezing, its origin, and its impact on frozen-thawed semen. Forty ejaculates were used. Semen was frozen using the classical freezing procedure. MPO concentrations were assayed in fresh semen, after centrifugation, and after cooling down to 4°C. Post-thaw MPO assay results and spermogram characteristics were determined. MPO immunocytochemistry was performed on 4 different ejaculates at each step of freezing procedure. MPO concentration increased after cooling down to 4°C and thawing compared with fresh semen. As temperature decreased, MPO was higher or tended to be higher in post-thaw poor quality samples. Nonsperm cells showed various degrees of MPO immunostaining and were observed as epithelial cells with nuclear pyknosis and keratinization. MPO immunostaining increased in medium and decreased in nonsperm cells during freezing. Our study shows that MPO concentration in equine semen increases when temperature decreases. We hypothesize that nonsperm cells present in fresh semen could release MPO.     

不合格血标本护理相关因素原因分析与对策

目的：结合临床实践经验,探讨临床检验中不合格血液标本的原因并制定相应的整改措施,降低血标本不合格率。方法：选取山东省肿瘤医院普外科2013年6月-12月入院患者的血标本,对不合格血标本进行原因分析,2014年1月-6月针对分析的问题进行改进,计算血标本不合格率。结果：通过分析上半年不合格血标本的原因并实施相应措施,下半年血标本不合格率由0.83%降低到0.54%。结论：根据检验科室筛选的不合格标本分析,规范护理人员操作,缩短物业人员运送标本的时间,避免途中剧烈震动,可以有效降低血标本不合格率。     

不同磷源及其配比对铜绿微囊藻生长和摄磷效应的影响

在实验室条件下研究了无机磷(磷酸氢二钾)与2种有机磷(β-甘油磷酸钠、三磷酸腺苷钠)不同配比对铜绿微囊藻(Microcystis aeruginosa)的生长效应以及微囊藻摄取不同磷形态[总溶解磷(total dissolved phosphorus, TSP)、溶解反应磷(soluble reactive phosphorus, SRP)和溶解有机磷(dissolved organic phosphorus, DOP)]的规律.结果表明,当无机磷与有机磷配比为6∶1时,对铜绿微囊藻生长最有利,对数生长期可维持6～8 d,其最大生物量达2.586×106个/mL;培养基中以有机磷作为唯一磷源时,铜绿微囊藻的生长最差;2种有机磷(β-甘油磷酸钠、三磷酸腺苷钠)对铜绿微囊藻生长的效果相似,铜绿微囊藻对SRP的摄取速度越快,其生长速率也越高(R=0 9995);待水中可利用磷耗尽后,β-甘油磷酸钠会在碱性磷酸酶作用下转化成可利用磷供藻生长,而三磷酸腺苷可直接分解成磷酸基供铜绿微囊藻生长.