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41.
Marine organisms have been shown to be a valuable source for biologically active compounds for the prevention and treatment of cancer, inflammation, immune system diseases, and other pathologies. The advantage of studying organisms collected in the marine environment lies in their great biodiversity and in the variety of chemical structures of marine natural products. Various studies have focused on marine organism compounds with potential pharmaceutical applications, for instance, as immunomodulators, to treat cancer and immune-mediated diseases. Modulation of the immune system is defined as any change in the immune response that can result in the induction, expression, amplification, or inhibition of any phase of the immune response. Studies very often focus on the effects of marine-derived compounds on macrophages, as well as lymphocytes, by analyzing the release of mediators (cytokines) by using the immunological assay enzyme-linked immunosorbent assay (ELISA), Western blot, immunofluorescence, and real-time PCR. The main sources are fungi, bacteria, microalgae, macroalgae, sponges, mollusks, corals, and fishes. This review is focused on the marine-derived molecules discovered in the last three years as potential immunomodulatory drugs. 相似文献
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43.
福氏志贺菌gyrA和parC基因QRDR序列的测定与同源性分析 总被引:2,自引:0,他引:2
测定了我国痢疾流行病原志贺菌福氏2 a 3 0 1株与喹喏酮类药物耐药性相关的 gyr A( DNA旋转酶 A亚单位 )和 par C(拓扑异构酶IVA亚单位 )基因 ,并将 gyr A和 par C基因QRDR(喹喏酮类药物耐药性决定区 )核苷酸序列与几种动物和人的病原菌进行了同源性和遗传进化比较分析。结果显示 ,志贺菌福氏 2 agyr A和 par C基因 QRDR序列分别与宋内氏志贺菌、大肠杆菌 O1 57、大肠杆菌 K1 2、阴沟肠杆菌、坂口肠道杆菌、鼠伤寒沙门氏菌、副伤寒沙门氏菌、肺炎克氏杆菌、产道克氏杆菌、空肠弯曲杆菌、弗氏柠檬酸杆菌具有显著同源性(均大于 88% ) ,表明 gyr A和 par C是这些细菌中的看家基因 ,推测在各种细菌中具有类似的起源 ,因此对在不同细菌之间进行喹喏酮类药物耐药性的研究非常有利。 QRDR的遗传进化分析表明 ,同属或相近属细菌 gyr A或 par CQRDR的遗传距离明显接近 ,其中与大肠杆菌属的距离最近 ,认为可列到同一个属 ,结果有力支持了近年提出的大肠杆菌与志贺菌属于同族细菌的理论。研究对理解喹喏酮类药物耐药性的分子机理及其与细菌遗传进化之间的关系具有重要意义 相似文献
44.
应用FA及PPA-ELISA技术对猪瘟的检测 总被引:3,自引:0,他引:3
用免疫荧光抗体诊断技术及单克隆抗体纯化酶联免疫吸附试验诊断技术对疑为猪瘟病毒感染猪进行了检测 ,重点阐述了 2种方法的技术原理和操作过程。通过用免疫荧光抗体诊断技术检测了 5份病料 ,其中有 2份为阳性 ,阳性率为 40 % ;用单克隆抗体纯化酶联免疫吸附试验诊断技术检测了猪瘟血清抗体 ,在 40头母猪血清样品中 ,猪瘟弱毒抗体效价 OD值较高 ,有 1 0 0 %的保护率 ,但是发现母猪群中有 1 0 %隐性猪瘟感染 ,其强毒抗体效价 OD值大于 0 .5,体内带有猪瘟病毒。结果及过程表明此两种诊断方法检测快速、鉴别准确、分辨率高 相似文献
45.
Characterization of ileal bacterial microbiota in newly-weaned pigs in response to feeding lincomycin, organic acids or herbal extract 总被引:1,自引:1,他引:1
Joshua Gong Hai Yu Ting Liu Mei Li Weiduo Si Cornelis F.M. de Lange Cate Dewey 《Livestock Science》2008,116(1-3):318-322
The changes of ileal bacterial microbiota in pigs during the first 2 weeks post-weaning in response to feeding lincomycin, organic acids or herbal extract were characterized using 16S rRNA gene-based PCR-denaturing gradient gel electrophoresis (DGGE) profiling, DNA sequencing, and real-time PCR (QPCR) techniques. Both time post-weaning and the dietary treatments resulted in a shift of the microbiota composition. While time post-weaning mostly influenced Clostridium group, the feed additives increased the population of Lactobacillus and related lactic acid bacteria by ≥ 3-fold. 相似文献
46.
山羊肺淋巴系的研究 总被引:2,自引:0,他引:2
24例山羊肺经肺实质和肺胸膜下注射30%普鲁士蓝氯仿溶液,剖查其器官内淋巴管及淋巴流向。结果表明,山羊肺的器官内淋巴管有浅淋巴管和深淋巴管2种。左肺尖叶淋巴管注入左支气管肺淋巴结和气管支气管左淋巴结,左肺心叶淋巴管注入左支气管肺淋巴结、气管支气管左淋巴结和气管支气管中淋巴结,左肺膈叶淋巴管注入气管支气管左淋巴结、气管支气管中淋巴结和纵隔后淋巴结;右肺尖叶淋巴管注入右支气管肺淋巴结和气管支气管前淋巴结,右肺心叶淋巴管注入右支气管肺淋巴结和气管支气管右淋巴结,右肺膈叶淋巴管注入气管支气管右淋巴结、气管支气管中淋巴结和纵隔后淋巴结,右肺副叶淋巴管注入右支气管肺淋巴结、气管支气管中淋巴结和纵隔后淋巴结。 相似文献
47.
Mystery swine disease in The Netherlands: the isolation of Lelystad virus. 总被引:115,自引:0,他引:115
G Wensvoort C Terpstra J M Pol E A ter Laak M Bloemraad E P de Kluyver C Kragten L van Buiten A den Besten F Wagenaar 《The Veterinary quarterly》1991,13(3):121-130
In early 1991, the Dutch pig-industry was struck by the so-called mystery swine disease. Large-scale laboratory investigations were undertaken to search for the etiological agent. We focused on isolating viruses and mycoplasmas, and we tested paired sera of affected sows for antibodies against ten known pig viruses. The mycoplasmas M. hyosynoviae, M. hyopneumoniae, and Acholeplasma laidlawii, and the viruses encephalomyocarditis virus and porcine enterovirus types 2 and 7 were isolated from individual pigs. An unknown agent, however, was isolated from 16 of 20 piglets and from 41 of 63 sows. This agent was characterised as a virus and designated Lelystad virus. No relationship between this virus and other viruses has yet been established. Of 165 sows reportedly afflicted by the disease, 123 (75 per cent) seroconverted to Lelystad virus, whereas less than 10 per cent seroconverted to any of the other virus isolates or to the known viral pathogens. Antibodies directed against Lelystad virus were also found in pigs with mystery swine disease in England, Germany, and in the United States. We conclude that infection with Lelystad virus is the likely cause of mystery swine disease. 相似文献
48.
Sasanelli M Paradies P Otranto D Lia RP de Caprariis D 《The Journal of small animal practice》2008,49(8):417-420
Angiostrongylosis was diagnosed in a dog presenting with haemothorax on the basis of detection of Angiostrongylus vasorum first-stage larvae both in the pleural effusion and in faeces. A one-year-old, male, mixed-breed dog was presented with fever, depression and persistent cough of one month's duration. Clinical examination revealed temperature of 39.5 degrees C, loud bronchovesicular sounds on thoracic auscultation and attenuated cardiac sounds. Thoracic radiographs showed a moderate bilateral pleural effusion and a diffuse interstitial pulmonary pattern, with an alveolar pattern in one lobe. Routine haematology revealed anaemia and leucocytosis with eosinophilia, basophilia and thrombocytopenia. Coagulation assays showed a consumptive coagulopathy resembling disseminated intravascular coagulation. The relationship between haemothorax and the presence of A vasorum larvae in the pleural effusion is discussed. The dog was successfully treated with fenbendazole until negative for larvae on faecal examination. This case report indicates that A vasorum infection should be considered as a possible aetiological cause of haemothorax in dogs. 相似文献
49.
da Silva Andrade G Bruhn FR Rocha CM Guimarães Ade S Gouveia AM Guimarães AM 《Veterinary parasitology》2012,188(1-2):168-171
The aim of this study was to determine the prevalence and risk factors associated with infection due to Neospora caninum in serum samples from 488 sheep originating from 63 farms in 63 municipalities distributed across eight of the twelve mesoregions of the state Minas Gerais, southeastern Brazil. For detection of N. caninum the sheep serum samples were subjected to the indirect fluorescence antibody test (IFAT ≥ 50). To identify the risk factors associated with infection due to N. caninum a questionnaire was filled out for each herd by interviewing, the individual responsible for the herd, demanding information on the general characteristics of the property. Sixty-four sheep sera (13.1%; 95% CI=10.3-16.4) presented IgG-specific anti-N. caninum antibodies with the following titers: 50 (49; 76.6%), 100 (7; 10.9%), 200 (4; 6.2%), 400 (3; 4.7%) and 800 (1; 1.6%). The prevalence of infected sheep per mesoregion ranged from 0 to 28.1%. Out of the 63 farms sampled, 31 (49.2%; 95% CI=36.4-62.1) presented at least one seropositive sheep. No significant association was found between the presence of anti-N. caninum antibodies and the risk factors evaluated on the farms, except for the mesoregion variable (p=0.004; OR=0.429; CI95%=0.182-1.008). These results indicate that there is a need for additional research to define the epidemiological importance of this parasite as a cause of reproductive problems in sheep herds in Minas Gerais. 相似文献
50.
Pedotransfer functions to assess adsorbed phosphate using iron oxide content and magnetic susceptibility in an Oxisol
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L. A. Camargo J. Marques Jr. G. T. Pereira L. R. F. Alleoni A. S. R. de S. Bahia D. De B. Teixeira 《Soil Use and Management》2016,32(2):172-182
Adsorbed phosphate in soils can be chemically extracted; however, this process is both time‐consuming and not cost‐effective if large numbers of samples have to be analysed. Indirect assessment of adsorbed phosphate by pedotransfer functions (PTFs) can help optimize fertilizer strategies. This study aimed to evaluate the spatial variability of adsorbed phosphate (Pads), iron oxides and magnetic susceptibility (MS) in oxisols and to calibrate PTFs to predict Pads. A total of 308 soil samples were collected from Hapludox and Eutrudox soils formed from sandstone in Brazil. The contents of clay (196–607 g/kg), iron oxides (40–165 g/kg), MS (1.2–29 × 10?6 m3/kg) and Pads (327–842 mg/kg) were in the range of typical values for these highly weathered soils. This study showed that the attributes studied were spatially dependent. Geomorphic surfaces enabled understanding of spatial variability and helped to develop a more efficient sampling scheme to calibrate PTFs. Moreover, the adsorbed phosphate in these oxisols could be predicted by a PTF using iron oxides and MS as predictors. The MS attribute enabled the most accurate prediction (concordance coefficient = 0.95, root‐mean‐square error = 46 mg/kg and relative improvement in root‐mean‐square error = ?4.12) of spatial variability through PTF compared to other predictors. 相似文献