Molecular simulations probing Kushecarpin A as a new lipoxygenase inhibitor

Zizyphus oxyphylla Edgew is used in Pakistan as a folk medicinal remedy for inflammatory conditions, pains especially rheumatic pain, fevers, allergy and diabetes. The aim of the current study was to scientifically validate the folk use of Z. oxyphylla Edgew by using the isolated compound in vitro and in vivo levels. Kushecarpin A was isolated from ethyl acetate fraction of the plant crude extract. Molecular docking simulations predicted Kushecarpin A as a potential new lipoxygenase (LOX) inhibitor. Kushecarpin A showed significant lipoxygenase inhibition (IC50: 7.2 ± 0.02 μM) thus validated computational prediction. It also exhibited significant and highly significant inhibition (p < 0.05 and p < 0.01) of carrageenan-induced hind paw oedema at the doses of 5, 10 and 20 mg/kg. Kushecarpin A seems to be a potentially new anti-inflammatory compound responsible for anti-inflammatory activities of Z. oxyphylla Edgew. In vitro and in vivo anti-inflammatory inflammatory activities were found in good agreement with the folk medicinal use of Z. oxyphylla Edgew in inflammatory disorders.     

Pythium yorkensis sp. nov., a potential soybean pathogen from southeastern Pennsylvania,USA

Biodiversity surveys of natural as well as agricultural ecosystems commonly reveal novel isolates. A new species, Pythium yorkensis sp. nov., is reported here, isolated from field soil during a survey of oomycete diversity in symptomless soybean across southeastern Pennsylvania. Molecular data from both mitochondrial and nuclear loci support a unique phylogenetic position for the isolates collected, and morphological features distinguish this new species from close relatives in Pythium clade J. Pathogenicity assays suggest that this new species may be a potential agricultural pathogen, as isolates were mildly aggressive on soybean. This study highlights the continued need for culture‐based surveys in collaboration with high‐throughput sequence‐based approaches for environmental sampling.     

Molecular characterisation of Theileria equi and risk factors associated with the occurrence of theileriosis in horses of Punjab (Pakistan)

Theileria equi (T. equi) is an obligate intra- and extra-erythrocytic parasite that causes equine theileriosis (ET) in equids. Equine theileriosis is considered a notifiable disease of global significance, a major constraint to the international movement of horses, and endemic in many countries. This disease may be difficult to diagnose, as it can produce variable and nonspecific clinical signs. A cross-sectional study was designed for the molecular characterisation of T. equi and to investigate the associated risk factors of ET accompanied by its consequences on haematological and sero-biochemical parameters. A convenience sampling of 500 blood samples were collected from ET suspect horses from January to December 2017. PCR was performed on all blood samples targeting the 18S rRNA gene of T. equi followed by sequencing; 9% animals tested positive with confirmed sequences. The isolates of this study showed high homology with Cuban, Russian and Brazilian isolates of T. equi (accession numbers KY111762.2 , MG551915.1 and KY952237.1 , respectively). Based on multivariate analysis, the principal risk factors consisted of absence of dogs on the premises and presence of tick infestation. The haemato-biochemical parameters showed a decrease in granulocytes and erythrocytes, and an increase in lymphocytes, monocytes, mean corpuscular volume, mean corpuscular haemoglobin, mean platelet volume, glucose, phosphorus and aspartate aminotransferase in positive horses. This is the first study which identified ET in Punjab (Pakistan) using molecular techniques and risk factors together with the haemato-biochemical variations in horses.     

Preference and performance of <Emphasis Type="Italic">Plutella xylostella</Emphasis> (L.) (Lepidoptera: Plutellidae) on canola cultivars

The diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae), is an important insect pest of canola, Brassica napus L., in Ardabil, Iran. Host plant resistance is an essential component of integrated management of P. xylostella. We investigated the preferences and performance of P. xylostella on nine commercial cultivars of canola, namely Zarfam, RGS003, Adder, Okapi, Opera, Hyola401, Ebonite Option500 and Elite under greenhouse conditions at 23 ± 1°C, 50 ± 5% RH and 14L:10D. In free-choice situation, oviposition was lowest on Opera (23.5) and highest on Zarfam (44.7). In the life table study, fecundity of new generation female moths was lowest on Opera (95.4) and highest on Adder (145.7). Survival from egg to adult was significantly lower on Opera, Option500 and Hyola401 than on other tested cultivars. The intrinsic rate of natural increase (r _m) and population growth rate (λ) were lowest on Opera and highest on Zarfam. The generation time (T) was shortest on Zarfam (17.2 days) and longest on Hyola401 (19.9 days) whereas doubling time (DT) was longest on Opera (3.9 days) and shortest on Zarfam (3.0 days). Our results clearly suggest that Opera was the most resistant host among the tested cultivars and has the potential to be used in the integrated management of P. xylostella.     

Endophytes from maize with plant growth promotion and biocontrol activity under drought stress

In the present study, 39 endophytic bacteria were isolated from different crops with main focus on maize roots and seeds. Endophytes were screened for drought stress tolerance, plant growth promoting (PGP) traits and antifungal activity. Out of 39 isolates, 32 could show drought tolerance up to–1.02 matric potential (MPa) and exhibited most of the plant growth promoting traits. But, only five isolates could show antagonistic activity against plant fungal pathogens. Based on the results, 10 promising isolates namely FTR, NFTR, FMZR9, FMZR2, MZ30V92, MRC12, MRC31, MRC33, MRC41 and MRR2 were selected and identified using biochemical and 16S rDNA gene sequencing as Pseudomonas aeruginosa (strains FTR and NFTR), Pseudomonas monteilii (strain FMZR2), Pseudomonas putida (strain FMZR9), Acitenobacter brumalii (strain MZ30V92), Enterobacter asburiae (strain MRC12), Sinorhizobium meliloti (strain MRC31), Pseudomonas thivervalensis (strain MRC33), Pseudomonas fulva (strain MRC41), and Pseudomonas lini (strain MRR2). Further, at–1.02 MPa all the 10 isolates showed PGP traits, and 3 isolates (FTR, NFTR and MRC12) showed antifungal activity. Thus, indicating that drought tolerant plant growth promoting antagonistic endophytic bacteria (PGPAE) helps in plant growth and disease management under drought stress.     

Bacteria wilt of Casuarina equisetifolia in India

Pseudomonas solanacearum (Smith) was consistently isolated from the wilted plants of Casuarina equisetifolia. The isolate gave positive reaction to oxidase, catalase and gas production from nitrate and negative reaction for arginine dihydrolase and acid was produced from various carbohydrates. Wilt produced on tomato, egg plant and capsicum indicated that the pathogen belongs to Race I and Biovar III of P. solanacearum which is a new record from India.     

Soil Salinization in Some Irrigated Areas of the Kingdom of Bahrain

Monitoring and assessment of agricultural land degradation is of vital importance for better land and water management planning and reclamation. It requires setting baseline information and basic analysis at specific time and space. About 33 geo-referenced soil sampling spots were selected in two agricultural production locations in the Kingdom of Bahrain to assess the status and preliminary causes of land degradation. Soil samples were taken from 13 sites in Diraz location while 19 samples were taken from Budayyi location. The samples were taken to 90 cm depth at 30 cm intervals. Standard procedures were followed to determine soil physiochemical properties. In addition, field observations on farm condition, distance from the sea, method of irrigation and irrigation water source were taken. Some of the soil samples were deliberately taken from outside the irrigated basins among trees compared with samples taken from inside the actively growing area for comparison. The results indicated that the salinity level was significantly （P 〈 0.001） higher at the 0-30 cm soil depth compared with 30-60 cm or 60-90 cm depths in both locations. The distance from the sea did not show clear correlation with surface soil salinity in Budayyi area compared with Diraz. Both locations showed significantly higher salinity levels on samples taken outside the actively growing areas compared with those taken from within. The effect is more prominent at the 0-30 cm depth. The observed variability on salinity levels may be attributed to farm management practices and deteriorating quality of ground water. Thus, agricultural land degradation in Bahrain cannot be attributed to ground water deterioration alone. The use of tertiary treated sewage water （TSE） may ease the pressure on ground water, but the pH of the TSE should be carefully monitored and managed with proper studies on leaching requirements to avoid further salinity complications.     

Inferring biological soil crust successional stage using combined PLFA, DGGE, physical and biophysiological analyses

Arid areas are highly sensitive to climate change and are ideal model systems to study the potential impact of climate change on species' community structure. Biological soil crust (BSC) formation plays an ecological role in a number of key processes in the development of dry ecosystems. It was hypothesized that BSC succession and function are affected by aridity level and limited by rainfall. Furthermore, it is possible to infer the direction of the BSC succession based on aridity level, and the latter can imitate future climate change scenarios. The objectives of this study were to investigate the microbial biomass and diversity of the BSC structure in three sites differing in aridity level (semiarid, arid and hyper-arid), by combining physical and biophysiological measurements with 16S rRNA gene fragment and phospholipid fatty acid (PLFA) analyses. Physical and biophysiological parameters of the BSC were significantly influenced by aridity level. Total protein and polysaccharide contents were strongly correlated with total PLFA-based microbial biomass. Gram-positive biomarkers and microbial biomass were significantly higher in the wettest (semiarid) site than in the driest (hyper-arid) one. Multivariate-analysis based ordination of the PLFA data segregated the cluster of semiarid data from that of the hyper-arid site, while data from the arid site were dispersed between the two. The phylogenetic distribution of prominent 16S rRNA bacterial gene sequences along the aridity levels was in agreement with the PLFA analysis: the hyper-arid site was dominated by the cyanobacterium Microcoleus vaginatus, while diverse populations of cyanobacteria and soil bacteria were found in the other sites. These complementary tools allowed a simple and sensitive measurement of the influence of aridity levels on BSC successional stage. The results demonstrate that different aridity levels correspond to different BSC successional stages and those differences can be used as parameters for global change scenarios.     

Novel technique for definite blastomere inhibition and distribution of maternal RNA in sterlet Acipenser ruthenus embryo

Fisheries Science - The cleavage pattern of a vertebrate’s embryo is either holoblastic (complete) or meroblastic (partial). Sturgeon and other basal bony fishes represent a transition of the...     

Effects of feeding rates on growth and production of Nile tilapia, common carp and silver carp polycultured in fertilized ponds

Abstract In an attempt to identify appropriate feeding rates for multispecies of fish raised in fertilized earthen ponds, the present work was conducted over a 19‐week experimental period to establish the growth performance, production and body composition of Nile tilapia, common carp and silver carp fed 0.0%, 0.5%, 1.0%, 3.0%, 5.0% biomass and to apparent satiation (treatments). Twelve ponds were stocked with a similar number and weight of each fish species. Two ponds were assigned to each of the treatments, and a 25% protein pelleted fish feed was used to feed fish at the specified rate of feeding. At the end of the experiment, growth, weight gain, survival, yield and body composition of fish groups were affected by the treatments. The economic effectiveness also varied among treatments. The most conspicuous attribute of the feeding rates was its lack of influence on growth (g day^?1), weight gain (g per fish), yield (kg ha^?1) or body composition of silver carp. The results of whole‐body proximate analysis indicated that various feeding rates had either an irregular pattern or no effects on the protein and ash gain per 100 g of fish body weight (bw) gain. The most notable exceptions were significant (P < 0.05) increases in body fat and gross energy gains in Nile tilapia, common carp and silver carp accompanied by decreases in percentages of moisture (but not in silver carp) as feeding rate increased. Among the six different feeding levels, feeding to apparent satiation (feed amount was equivalent to 2.67% of fish bw day^?1) appeared to be optimal, as it significantly (P < 0.05) supported the highest fish production, income and net profit compared with all other treatments except for the 3% feeding level, for which the differences in those measurements were comparable.