生菜叶片光谱红边参数对氮营养的响应特征分析

以不同氮素供应水平下的水培生菜为研究对象,采用光谱仪和叶绿素仪分别获得生菜叶片的漫反射光谱信息和叶绿素含量,通过提取生菜叶片光谱的红边参数,分析不同氮素供应水平下红边参数的变化规律,以及各个红边参数与生菜叶片叶绿素含量间的关系.结果表明,随氮营养供应水平的提高,生菜叶片各红边参数呈现不同的响应趋势.红边位置、最小振幅、红边面积以及红边振幅和最小振幅比值与生菜叶片SPAD值间均具有极显著的相关关系,其中以红边位置的相关程度最高,其相关系数为0.9420,均方根误差为1.803.可见,红边位置可以作为生菜叶片氮素营养诊断的一种有效指标.     

The role of ectomycorrhizal fungi in alleviating pine decline in semiarid sandy soil of northern China: an experimental approach

The decline of Mongolian pine (Pinus sylvestris var. mongolica) trees on sandy land in northern China has caused serious ecological concerns. Mongolian pine is an ectomycorrhizal fungus (ECM)-dependent species. Three ECM species (Boletus sp., Lactarius deliciosus and L.sp.) were collected from Mongolian pine plantation stands to test their beneficial effects on Mongolian pine seedlings and their responses to environmental factors such as pH, drought stress and temperature. The results indicated that ECM inoculation significantly increased the rate of ECM colonization and the length of seedling shoots. The three ECM could grow in a pH range from 4 to 7, but did not grow under heavy drought stress (?1.53 MPa). High temperatures (over 37 °C) caused death of ECM. When related to soil pH, soil water content and temperature in the Mongolian pine plantations, water conditions and temperature were unfavorable for ECM growth in surface soil, but suitable in deeper soil. Therefore, it was concluded that the failure of natural regeneration in Mongolian pine plantations might be influenced by the lack of ECM in the surface soil because of high temperatures causing ECM death. Moreover, the majority of the root area is distributed in deeper soil, which alleviates the stresses on ECM development and arrests pine decline; this is beneficial for tree growth.     

水稻雄性不育突变体012S-3的遗传分析和基因定位

雄性不育是水稻杂种优势利用的重要资源,对雄性不育现象的研究具有重要理论意义和实践价值。本研究以自然突变的水稻雄性不育突变体012S-3为试验材料,对其表型特征和花粉育性等进行调查,并构建遗传群体,利用分子标记对目的基因进行初步定位,然后应用基因组重测序技术对其进行精细定位。结果表明,012S-3是一个典型的无花粉普通型雄性不育材料,其不育性状受1对隐性核基因控制。初步定位分析目的基因与SSR标记RM6081存在连锁关系,其遗传距离约为34.4 c M;进一步的精细定位分析,找到3个候选基因:LOC_Os07g35880、LOC_Os07g35920和LOC_Os07g35940,其中LOC_Os07g35880和LOC_Os07g35940编码β-淀粉酶,属于水稻中新发现的花粉致死基因。该不育基因的成功定位为其进一步的分离克隆及其在水稻分子设计育种中的应用奠定了基础。     

2008年浙江余姚棉花品种比较试验

因近几年来流入浙江余姚市的棉花品种较多,为寻找适合余姚棉区更好的主栽品种,2008年引进各地优势品种共7个,进行比较试验。     

理论灌输模式的建构

运用模式方法研究高校思想政治教育是当前教育研究的崭新领域,对于提高高校思想政治教育的实效性具有重要意义.理论灌输模式作为高校思想政治教育模式的一种,包括教育者、受教育者、教育内容、教育手段四个要素和激发动机、理论传授、理论学习、检查巩固、实践运用、总结评价等操作环节,它主要应用于高校思想政治理论课模式、通识教育模式、政治理论学习模式和个别辅导模式等.     

城市空间增长效应评价方法——以重庆市为例

在城市空间快速增长背景下,开展城市空间增长效应评价研究,有助于解决城市空间增长中出现的问题,可为确定城市空间发展战略、制定空间发展政策提供依据。城市空间增长效应评价方法旨在定量、客观地判断城市空间增长过程中要素配置与运行是否合理、高效和公平。笔者建立了结构配置效应、经济配置效应和公平配置效应3个方面的评价体系,通过目标法对城市空间增长效应进行评价,得出空间增长效应评价函数。以重庆市为例进行实证分析,结果显示：重庆市2004-2013年间的城市空间增长效应总体呈上升发展趋势,结构性、公平性趋好;合理配置效应和经济配置效应波动显著,反映出城市建设面临的用地结构优化、用地效率提升等方面的问题。     

Effects of nitrogen and water stress on the rehydration,endogenous hormonal regulation and yield of maize

Water scarcity is known to be a strong limiting factor affecting maize grown and yield in cold semi-arid regions. Numerous studies have shown that rehydration improves maize growth. Our study aimed to explore the effects of rehydration treatments on maize growth and yield under water and nitrogen stress during different growth stages. We selected the drought-tolerant maize variety Nendan 19 (ND19) and subjected it to water stress during the V6 (sixth-leaf), R2 (filling) and R6 (maturity) growth stages and a rehydration treatment after each stress stage. Our results indicated that N1 (N100 kg N ha⁻¹) and N3 (N300 kg N ha⁻¹) treatments significantly increased the leaf moisture status relative to water content (RWC), bound water content (BWC), free water content (FWC) and water potential (WP)) at different growth stages. Similar trends were observed in the accumulation of plant leaf and root hormones (zeatin+zeatin riboside, indole-3-acetic acid, abscisic acid and gibberellic acid), photosynthetic pigments and chlorophyll fluorescence. However, under the same water stress conditions, they decreased as the N rate increased and reached a minimum value in the S3 (water stress for N3) treatments. In addition, with growth stage advancement and extension of the rehydration time, both showed a gradual upward trend. The results showed that to save water resources in the cold semi-arid region, rehydration treatments (R2S1 and R2S3) significantly increased the photosynthetic pigments and chlorophyll fluorescence parameters, leaf moisture status, biomass, 100-grain weight, hormone content, ear characteristics and grain yield of maize.     

Forest biomass and root–shoot allocation in northeast China

Temperate and boreal forests act as major sinks for atmospheric CO₂. To assess the magnitude and distribution of the sinks more precisely, an accurate estimation of forest biomass is required. However, the determinants of large-scale biomass pattern (especially root biomass) are still poorly understood for these forests in China. In this study, we used 515 field measurements of biomass across the northeast part of China, to examine factors affecting large-scale biomass pattern and root–shoot biomass allocation. Our results showed that, Picea & Abies forest and coniferous & broadleaf mixed forest had the highest mean biomass (178–202 Mg/ha), while Pinus sylvestris forest the lowest (78 Mg/ha). The root:shoot (R/S) biomass ratio ranged between 0.09 and 0.67 in northeast China, with an average of 0.27. Forest origin (primary/secondary/planted forest) explained 31–37% of variation in biomass (total, shoot and root), while climate explained only 8–15%, reflecting the strong effect of disturbance on forest biomass. Compared with shoot biomass, root biomass was less limited by precipitation as a result of biomass allocation change. R/S ratio was negatively related to water availability, shoot biomass, stand age, height and volume, suggesting significant effects of climate and ontogeny on biomass allocation. Root–shoot biomass relationships also differed significantly between natural and planted forests, and between broadleaf and coniferous forests. Shoot biomass, climate and forest origin were the most important predictors for root biomass, and together explained 83% of the variation. This model provided a better way for estimating root biomass than the R/S ratio method, which predicted root biomass with a R² of 0.71.     

美国车厘子组织培养试验

采用美国思加图车厘子室内盆栽萌发的嫩芽和老芽作为外植体,分析不同处理时间对车厘子外植体萌芽效果的影响;采用不同浓度的植物生长调节剂对其进行诱导增殖和生根培养,筛选最佳组培方法。结果表明：嫩芽是车厘子外植体的最佳选择,通过7 s酒精和0.1%升汞9 min消毒获得无菌体,污染率低、褐化率较低、萌芽率60%。芽诱导培养基为MS+6-BA 1.0 mg/L+NAA 0.2 mg/L,增殖系数为3.92。生根培养基为1/2MS+NAA0.6 mg/L,生根率为100%。移栽炼苗基质比例为V（腐殖土）∶V（红土）=4∶6,盖膜驯化,保湿温度（25±2）℃,湿度80%,成活率达90%以上。     

田面微地形对滴灌灌水均匀性的影响

首先分析了田面微地形对滴头实际工作压力的影响,田面越不平整,滴头实际工作压力与设计工作压力的差值越大,当田面局部高差大于滴头设计工作压力时,滴头流量等于零;然后提出了田面微地形产生的流量偏差率公式,并用泰勒级数展开;最后利用描述统计方法和独立样本T-检验的方法对余项进行分析,置信度为99%的余项取值区间为0.001￣0.002,说明余项能够被忽略,田面微地形产生的流量偏差率公式可以用简单的形式来表示。