野生种多毛、醋栗番茄与栽培种番茄的指纹图谱分析

本文利用CAPS和SSR两种分子标记技术,对野生种多毛和醋栗番茄与栽培种番茄的DNA指纹图谱进行了分析。结果表明,30对CAPS和10对SSR引物中,分别有19对和7对引物产生清晰明显且稳定的多态性条带。利用两种标记共获得了98个标记位点,分析表明SSR标记的检测效率高于CAPS标记。相对于栽培种而言,多毛番茄有16.3%的特异信息,醋栗番茄有10%的特异信息。该些标记为番茄遗传图谱的构建、重要基因的标记及遗传育种提供了有力工具。     

植树挖坑机钻头主轴扭转振动数学建模与求解

介绍国内外机械化植树造林机械发展状况,指出国内植树用挖坑机研究重点,利用空间运动学和机械振动理论以及弹性动力学原理着重分析建立钻头主轴扭转振动模型并用数值方法进行求解,同时系统地分析钻头主轴下端部件与土壤的相互作用力、钻头主轴重力及惯性力等因素的影响,给出动态的准随机边界条件和初始条件.为下一步的钻头动力学特性仿真及整机动态特性研究,合理预测和控制钻头的运动规律,改善钻头的动力学特性,提高钻头工作效率及改进设计方法提供新的理论依据.     

BTOPMC/SCAU分布式流域水文模型原理和系统设计

BTOPMC(block-wise use of TOPMODEL(TOPgraphic MODEL,基于地形的水文模型)with the Muskingum-Cunge method,分块应用TOPMODEL和马斯京根-康吉汇流方法)是一个具有较强物理基础的分布式流域水文模型,主要用于教学和流域研究,模型有待软件化,以便用于流域作业预报和水资源管理。该文采用多层架构体系设计了该模型的计算机系统,形成了BTOPMC/SCAU(south china agricultural university),系统由数据层、模型层、通信层、数据表达层和用户操作层组成,运行环境采用客户端/服务端模式。提供手动和自动2种参数率定模式,自动率定采用SCE-UA(shuffled complex evolution developed at university of arizona)全局最优算法,内置7种目标函数。采用Open MP(open multi-processing,开放式多处理)多核并发编程技术在产汇流模块实现了多核并行计算。系统为流域水文作业预报和水资源管理提供了精致的方法和简便工具。     

Effects of a Dual‐Frequency Frequency‐Sweeping Ultrasound Treatment on the Properties and Structure of the Zein Protein

We investigated the effects of a dual‐frequency frequency‐sweeping ultrasound (DFFSU) treatment on the functional properties and structure of zein. The solubility of ultrasound‐treated zein proteins increased slightly but significantly as the treatment time increased. The results showed that the DFFSU treatment had an obvious influence on the mean particle size and the size distribution. A significant (P < 0.05) increase in the size of the particles with respect to time was observed after a sonication time of more than 20 min in zein solutions. Differential scanning calorimetry results showed that sonication alters the thermal behavior of zein. Circular dichroism spectra showed a small increase in the percentage of ordered structure elements within the protein molecule. After 60 min of ultrasonication, the percentage of α‐helix structures increased by 0.9%, whereas the percentage of β‐sheets and β‐turns decreased by 0.5%. Microstructural analyses by scanning electron microscopy showed that several microholes appeared in the zein following ultrasonic pretreatment. Under the conditions investigated in this study, DFFSU treatment was found to affect the studied functional properties of the zein protein. This technology could be used to obtain improved functional properties in some protein samples.     

Structural identification of nonvolatile dimerization products of glucosamine by gas chromatography-mass spectrometry, liquid chromatography-mass spectrometry, and nuclear magnetic resonance analysis

The degradation profile of glucosamine bulk form stressed at 100 degrees C for 2 h in an aqueous solution was studied. Column chromatography of acetylated product mixture led to isolation of two pure compounds (1b and 2b) and a mixture of at least three isomers (3b). 1a and 2a were identified as 5-(hydroxymethyl)-2-furaldehyde (5-HMF) and 2-(tetrahydroxybutyl)-5-(3',4'-dihydroxy-1'-trans-butenyl)pyrazine, respectively, by utilizing a variety of analytical techniques, such as GC-MS, LC-MS, on-line UV spectrum, (1)H and (13)C NMR, and DEPT, as well as (1)H-(1)H COSY. 3a was identified as 2-(tetrahydroxybutyl)-5-(2',3',4'-trihydroxybutyl)pyrazine, commonly known as deoxyfructosazine. In addition, glucosamine solid dosage form was exposed to 40 degrees C/75% relative humility for 10 weeks. Methanol extract of glucosamine solid dosage form was analyzed after acetylation by LC-MS, resulting in degradants 3b and 4b. 3a and 4a were, therefore, determined as deoxyfructosazine and 2,5-bis(tetrahydroxybutyl)pyrazine (fructosazine), respectively. Furthermore, the mechanisms of formation of identified degradation products are proposed and briefly discussed.     

Effects of previous elemental sulfur applications on oxidation of additional applied elemental sulfur in soils

Oxidation of elemental sulfur (S⁰) in 20 Chinese agricultural soils was tested and the effects of previous S⁰ applications on the oxidation of additional applied S⁰ in selected soils were investigated using laboratory, greenhouse, and field experiments. Results showed that sulfur oxidative capacities presented great variability among 20 tested soils, with a coefficient of variation of 92.4%. There were no significant relationships between S⁰ oxidation and physical and chemical properties of the selected soil. Previous S⁰ amendment significantly increased the oxidation rate of additional applied S⁰. These stimulatory effects after the first applications of S⁰ were greater than those after two applications. The percent increase in S⁰ oxidation rate due to S⁰ pretreatment was negatively correlated with the oxidation capacities of soils before S⁰ pretreatments. The significant reduction of sulfur oxidation in autoclaved soils and significant increase in S⁰ oxidation after inoculation with S⁰-treated soil suspension demonstrated that microbial oxidation was mainly responsible for the enhancement of soil oxidation ability after previous S⁰ amendments.     

山茶属红山茶组植物种质资源

中国是山茶属红山茶组植物的分布中心,具有丰富的种质资源。该组植物作为著名观赏花卉和油料植物在中国有着悠久的栽培历史,是优良的乡土木本花卉,其观赏价值高,园林应用前景广阔,值得大力推广。加强对该组植物的研究和保护具有重要意义。     

适应机械化收获的田间油菜植株特性研究

油菜机械化收获过程出现的高损失率制约了油菜生产的发展,单纯从农机方面进行研究难以解决。该文从农机农艺相结合出发,对田间油菜植株特性进行测试研究。测试大田植株整体表现特性及不同密度下植株茎秆物理特性的田间表现,并在不同成熟度和催熟剂喷施情形下对油菜角果拉断力进行测试,获得了田间植株特性基础数据。结果表明适宜的种植密度有利于机械化收获作业,催熟剂的喷施对机收作业性能影响不大。研究获得了直播油菜的田间植株表现特征,同时为油菜机收配套农艺要求提供理论研究基础和参考依据。     

APQC知识管理框架的实践程序探析

研究了APQC知识管理框架的应用以及实践程序。