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81.
以大白菜卵圆型品种"锦抗1号"和直筒型品种"乡情80"为试材,在6个氮肥水平下,研究了叶片SPAD值与氮素营养诊断指标、施氮量的关系以及施氮量对产量的影响。结果表明:在大白菜莲座期和结球期,2个品种的叶片SPAD值与氮素营养诊断指标及施氮量有很好的相关性;2个生育期"锦抗1号"的临界SPAD值分别为40.45、42.45,"乡情80"的临界SPAD值分别为38.57、40.36;建立了基于叶片SPAD值的大白菜氮肥推荐施肥模型。综上所述,大白菜叶片SPAD值可以作为氮素营养诊断指标,并能推荐氮肥的施用。 相似文献
82.
为鉴定野生番茄的SpIDD1基因并探究其功能,以野生醋栗番茄‘PI365967’为试验材料,利用RT-PCR克隆得到SpIDD1基因的CDS序列,全长1 020 bp,编码339个氨基酸,与普通番茄参考基因组序列相比,SpIDD1只存在一个核苷酸位点的差异。蛋白序列比对和结构分析表明,Sp IDD1含有1个核定位信号和4个保守锌指结构域(C2H2·C2H2·C2HC·C2HC),是一个典型的IDD蛋白。系统进化分析表明,SpIDD1与马铃薯和辣椒的IDD蛋白亲缘关系最近。荧光定量PCR显示,SpIDD1在叶、顶芽和果实中的表达较高,并受干旱胁迫的显著诱导。利用TRV-VIGS系统构建了Sp IDD1的基因沉默载体,并成功侵染番茄。研究结果表明,SpIDD1作为一个典型的IDD基因,很可能参与了番茄的干旱胁迫。本研究为进一步研究该基因的功能及作用机理提供理论依据。 相似文献
83.
AIM: To explore the effect of poncirin on the growth of AGS gastric cancer cells and the underlying mechanism.METHODS: The effect of poncirin on AGS cell viability was measure by MTT assay. The cell cycle distribution and cell apoptosis were analyzed by flow cytometry. Nuclear staining with DAPI was used to reflect the morphological change of the AGS cells treated with poncirin. The protein levels of extrinsic apoptosis pathway-related proteins such as FasL, caspase-8, caspase-3 and PARP, and mitochondria-mediated intrinsic apoptosis pathway-associated proteins such as Bak, Bcl-xL, Bax and caspase-9 were determined by Western blot.RESULTS: Poncirin inhibited the viability of AGS gastric cancer cells in a time-and concentration-dependent manner (P<0.05). Poncirin induced accumulation of G1 DNA content and significantly increased total apoptosis in the AGS cells. Nuclear staining showed a dose-dependent increase in the number of apoptotic cells after treated with poncirin.The protein level of FasL was upregulated in a dose-dependent manner by treatment with poncirin. Poncirin significantly activated caspase-8 and caspase-3. Moreover, poncirin significantly induced the cleavage of PARP in a dose-dependent manner (P<0.05). In addition, the protein levels of Bcl-xL, Bax and Bak were unchanged after treated with different doses of poncirin. Furthermore, caspase-9 was not activated by poncirin treatment in the AGS cells.CONCLUSION: Poncirin has the anti-cancer effect via extrinsic apoptosis pathway to inhibit the growth of AGS gastric cancer cells, possibly making it a therapeutic agent for human gastric cancer treatment. 相似文献
84.
85.
南方建筑非透明围护结构热工与节能设计 总被引:1,自引:0,他引:1
如何兼顾冬夏两季建筑供暖空调负荷和能耗,保证室内热环境质量,确定最适宜的热工特性,是长期困扰中国南方建筑围护结构热工与节能设计的难点。通过对夏热冬冷和夏热冬暖地区建筑在采暖、空调与自然通风条件下动态热过程的分析,研究了这一地区围护结构热特性与能耗的制约关系,以及对室内热环境与建筑热稳定性的作用机理。在兼顾冬季保温与夏季隔热的情况下,将建筑全年能耗作为控制目标,从室内热环境质量、节能效果、围护结构的安全性、可靠性、经济性和实用性等角度评价目前所采用的围护结构节能技术存在的问题,提出一种适宜南方气候的建筑围护结构热特性指标及构造形式。 相似文献
86.
Glomalin-related soil protein(GRSP)sequesters large amounts of carbon and plays important roles in maintaining terrestrial soil ecosystem functions and ecological restoration;however,little is known about GRSP variation in 1-m soil profiles and its association with stand characteristics,soil properties,and climatic conditions,hindering GRSP-related degraded soil improvement and GRSP evaluation.In this study,we sampled soils from 1-m profiles from poplar(Populus spp.)shelterbelts in Northeast China.GRSP contents were 1.8–2.0 times higher in the upper 40 cm soil layers than at 40–100 cm.GRSP-related soil organic carbon(SOC)sequestration in deeper soil layers was*1.2 times higher than in surface layers.The amounts of GRSP-related nutrients were similar throughout the soil profile.A redundancy analysis showed that in both surface and deeper layers,soil properties(pH,electrical conductivity,water,SOC,and soil nutrients)explained the majority of the GRSP variation(59.5–84.2%);the second-most-important factor in GRSP regulation was climatic conditions(temperature,precipitation,and altitude),while specific shelterbelt characteristics had negligible effects(<5%).Soil depth and climate indirectly affected GRSP features via soil properties,as manifested by structural equation model analysis.Our findings demonstrate that GRSP is important for carbon storage in deep soils,regardless of shelterbelt characteristics.Future glomalin assessments should consider these vertical patterns and possible regulating mechanisms that are related to soil properties and climatic changes. 相似文献
87.
滇东北4种典型筇竹林凋落物的持水性 总被引:1,自引:1,他引:0
以滇东北4种典型筇竹林为研究对象,对比分析不同类型筇竹林凋落物储量及持水性能,从而为滇东北筇竹林生态可持续经营提供理论依据和实践指导。研究结果表明,凋落物储量为人工筇竹-黄皮树混交林(7.61 t/hm2) > 天然筇竹-人工黄皮树混交林(6.61 t/hm2) > 天然筇竹-人工厚朴混交林(5.73 t/hm2) > 天然筇竹纯林(5.23 t/hm2)。凋落物最大持水量为天然筇竹-人工厚朴混交林(3.45 t/hm2) > 人工筇竹-黄皮树混交林(3.22 t/hm2) > 天然筇竹-人工黄皮树混交林(2.89 t/hm2) > 天然筇竹纯林(2.69 t/hm2)。4种类型筇竹林凋落物吸水速率均随着浸泡时间延长而逐渐趋于一致,筇竹混交林凋落物吸水速率高于筇竹纯林。凋落物的总有效拦蓄量为人工筇竹-黄皮树混交林(9.74 t/hm2) > 天然筇竹-人工厚朴混交林(8.95 t/hm2) > 天然筇竹-人工黄皮树混交林(7.73 t/hm2) > 天然筇竹纯林(6.23 t/hm2)。 相似文献
88.
89.
In this review, we summarized the distribution of the chemically investigated Oceanapia sponges, including the isolation and biological activities of their secondary metabolites, covering the literature from the first report in 1989 to July 2019. There have been 110 compounds reported during this period, including 59 alkaloids, 33 lipids, 14 sterols and 4 miscellaneous compounds. Besides their unique structures, they exhibited promising bioactivities ranging from insecticidal to antibacterial. Their complex structural characteristics and diverse biological properties have attracted a great deal of attention from chemists and pharmaceuticals seeking to perform their applications in the treatment of disease. 相似文献
90.
Qi Zeng Yuchan Chen Junfeng Wang Xuefeng Shi Yihao Che Xiayu Chen Weimao Zhong Weimin Zhang Xiaoyi Wei Fazuo Wang Si Zhang 《Marine drugs》2022,20(2)
Three new metabolites, including a cyclic tetrapeptide asperhiratide (1), an ecdysteroid derivative asperhiratine (2), and a sesquiterpene lactone asperhiratone (3), were isolated and identified from the soft coral-derived fungus Aspergillus hiratsukae SCSIO 5Bn1003, together with 10 known compounds. Their structures were elucidated via spectroscopic analysis, X-ray diffraction analysis, and electronic circular dichroism calculations. In addition, the absolute configuration of 1 was determined by Marfey’s technique and an analysis of the acid hydrolysates using a chiral phase HPLC column. Among all the compounds, 6 and 8 showed medium cytotoxic activities against four tumor cell lines (SF-268, HepG-2, MCF-7, and A549), with IC50 values ranging from 31.03 ± 3.04 to 50.25 ± 0.54 µM. Meanwhile, they strongly inhibited α-glucosidase activities, with IC50 values of 35.73 ± 3.94 and 22.00 ± 2.45 µM, which were close to and even stronger than the positive control acarbose (IC50 = 32.92 ± 1.03 µM). Compounds 6–8 showed significant antibacterial activities against Bacillus subtilis, with MIC values of 10.26 ± 0.76 µM, 17.00 ± 1.25 µM, and 5.30 ± 0.29 µM, respectively. Compounds 9 and 12 exhibited potent radical scavenging activities against DPPH, with IC50 values of 12.23 ± 0.78 µM and 7.38 ± 1.16 µM. In addition, asperhiratide (1) was evaluated for anti-angiogenic activities in the in vivo zebrafish model, which showed a weak inhibitory effect on intersegmental vessel (ISV) formation. 相似文献