全文获取类型
收费全文 | 49528篇 |
免费 | 2361篇 |
国内免费 | 5270篇 |
专业分类
林业 | 5982篇 |
农学 | 8084篇 |
基础科学 | 4611篇 |
8176篇 | |
综合类 | 14257篇 |
农作物 | 2519篇 |
水产渔业 | 1847篇 |
畜牧兽医 | 6756篇 |
园艺 | 1582篇 |
植物保护 | 3345篇 |
出版年
2024年 | 103篇 |
2023年 | 419篇 |
2022年 | 1211篇 |
2021年 | 1945篇 |
2020年 | 1701篇 |
2019年 | 1789篇 |
2018年 | 1193篇 |
2017年 | 1643篇 |
2016年 | 1647篇 |
2015年 | 2291篇 |
2014年 | 2116篇 |
2013年 | 2619篇 |
2012年 | 3114篇 |
2011年 | 3411篇 |
2010年 | 3201篇 |
2009年 | 2975篇 |
2008年 | 2770篇 |
2007年 | 2789篇 |
2006年 | 2695篇 |
2005年 | 2680篇 |
2004年 | 1190篇 |
2003年 | 986篇 |
2002年 | 795篇 |
2001年 | 831篇 |
2000年 | 1018篇 |
1999年 | 1339篇 |
1998年 | 1192篇 |
1997年 | 1007篇 |
1996年 | 939篇 |
1995年 | 867篇 |
1994年 | 865篇 |
1993年 | 771篇 |
1992年 | 710篇 |
1991年 | 582篇 |
1990年 | 484篇 |
1989年 | 390篇 |
1988年 | 260篇 |
1987年 | 186篇 |
1986年 | 92篇 |
1985年 | 74篇 |
1984年 | 51篇 |
1983年 | 38篇 |
1982年 | 36篇 |
1981年 | 23篇 |
1980年 | 25篇 |
1979年 | 10篇 |
1978年 | 12篇 |
1977年 | 9篇 |
1965年 | 9篇 |
1963年 | 9篇 |
排序方式: 共有10000条查询结果,搜索用时 31 毫秒
991.
Dietary n‐3 highly unsaturated fatty acids affect the biological and serum biochemical parameters,tissue fatty acid profile,antioxidation status and expression of lipid‐metabolism‐related genes in grass carp,Ctenopharyngodon idellus 下载免费PDF全文
A 95‐day feeding trial was conducted to determine the effects of n‐3 highly unsaturated fatty acids (n‐3 HUFA) on the growth, antioxidation and lipid metabolism in grass carp (Ctenopharyngodon idellus). Two isonitrogenous and isolipidic diets were formulated with either lard oil (LO) or fish oil (FO) as the main lipid source. The results showed that the intraperitoneal fat (IPF) ratio was significantly lower (P < 0.05) in FO group. The concentration of n‐3 HUFA in muscle, hepatopancreas and IPF was significantly higher in FO group (P < 0.05). The serum low‐density lipoprotein (LDL) content was significantly lower (P < 0.05), and glucose (GLU) content was significantly higher (P < 0.05) in FO group. The serum total superoxide dismutase (T‐SOD) activity was significantly higher (P < 0.05) in FO group, consistent with the serum malondialdehyde (MDA) content. The gene expression of IPF fatty acid synthase (FAS), acetyl‐CoA carboxylase (ACC), sterol regulatory element‐binding protein (SREBP‐1) and peroxisome proliferator‐activated receptor γ (PPARγ) was significantly lower (P < 0.05) and that of peroxisome proliferator‐activated receptor α (PPARα) was significantly higher (P < 0.05) in FO group compared with LO group. Similar trends were found in the hepatopancreas, except for PPARγ. It is suggested that n‐3 HUFA could inhibit lipid accumulation in grass carp by affecting the expression of lipid‐metabolism‐related genes. 相似文献
992.
达里湖和岗根湖东北雅罗鱼和鲫四种同工酶的比较研究 总被引:3,自引:0,他引:3
比较了达里湖和岗根湖的东北雅罗鱼(Leuciscus waleckii)和鲫(Carassius auratus)肌肉、肝脏、心肌组织中过氧化物酶(POD)、酯酶(EST)、乳酸脱氢酶(LDH)、苹果酸脱氢酶(MDH)的差异。结果显示:达里湖中东北雅罗鱼和鲫在心脏和肝脏中POD、EST含量均高于岗根湖,而肌肉中含量相对低些;MDH、LDH在三种组织中的含量达里湖中的东北雅罗鱼和鲫也高于岗根湖。由试验结果可知,东北雅罗鱼和鲫心肌和肝脏中四种同工酶的含量高于岗根湖,肌肉中四种同工酶的含量相对稳定。 相似文献
993.
Junde Chen Ying Liu Ruizao Yi Long Li Ran Gao Nuohua Xu 《Journal Of Aquatic Food Product Technology》2017,26(1):86-94
Acid soluble collagen was extracted from the scales of lizardfish and characterized with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and amino acid analysis. After 8 h of collagen hydrolysis, hydrolysates had an estimated degree of hydrolysis (DH) from 4 ± 0.05 to 25 ± 0.63% (p < 0.05). All hydrolysates had angiotensin converting enzyme inhibitory and antioxidant activity. These activity levels showed little change after treatment with gastrointestinal proteases. Results indicated that the lizardfish by-products may be improved by enzymatic treatment with acid-soluble collagen from lizardfish scales. 相似文献
994.
H.‐R. Yu Q. Zhang H. Cao X.‐Z. Wang G.‐Q. Huang B.‐R. Zhang J.‐J. Fan S.‐W. Liu W.‐Z. Li Y. Cui 《Aquaculture Nutrition》2013,19(2):139-147
Apparent digestibility coefficients (ADCs) of dry matter, crude protein, crude lipid, gross energy, phosphorus and amino acids in Peruvian fish meal, poultry by‐product meal, meat and bone meal, spray‐dried blood meal, hydrolysed feather meal, corn gluten meal, soybean meal, peanut meal, cottonseed meal and rapeseed meal were determined for juvenile snakehead (Ophiocephalus argus) with initial mean body weight of 78.1 g. A reference diet and test diets that consisted of a 70 : 30 mixture of the reference diet to test ingredient were used with 5 g kg?1 Cr2O3 as an external indicator. Fish meal, poultry by‐product meal and corn gluten meal had higher ADCs of dry matter, crude protein, and gross energy among ingredients tested. Dry matter ADCs ranged 61.9–81.5% for animal ingredients and corn gluten meal and ranged 52.2–68.0% for soybean meal, peanut meal, cottonseed meal and rapeseed meal. Energy ADCs of ingredients followed similar trends to differences in dry matter digestibility. Protein ADCs of animal and plant ingredients ranged 73.6–92.8% and 75.3–85.6%, respectively. Amino acid ADCs generally reflected protein digestibility. Lipid ADCs were relatively high for the ingredients tested. Phosphorus ADCs of animal and plant ingredients ranged 39.5–65.2% and 38.7–57.1%, respectively. 相似文献
995.
996.
Cryopreservation of strip spawned sperm using non‐programmable freezing technique in the blue mussel Mytilus galloprovincialis 下载免费PDF全文
In this study, a non‐programmable freezing technique has been developed with the strip spawned blue mussel (Mytilus galloprovincialis) sperm. The key parameters optimized including (1) cryoprotectant agents (CPAs); (2) cooling temperature; (3) thawing temperature; (4) sugar and amino acid supplementation and (5) sperm to egg ratio. The fertilization rate and/or integrity of plasma membrane and acrosome were used as sperm quality assessment indicators. The highest post‐thaw sperm fertilization rate of 95% was achieved, when sperm were cryopreservated in 8% dimethyl sulfoxide at 7.8 cm above the liquid nitrogen surface and thawed in a 60°C seawater bath. The addition of glucose, sucrose or trehalose in dimethyl sulfoxide did not, whereas 0.8% glycine did significantly improve the post‐thaw sperm fertilization rates. The fluorescent evaluation has demonstrated that the addition of glycine significantly improved the post‐thaw sperm acrosome integrity, revealing a positive role of glycine in the improvement of post‐thaw sperm quality in blue mussels. 相似文献
997.
Live, attenuated Vibrio anguillarum strains can serve as vectors for the delivery of heterologous antigens for development of multivalent recombinant vaccines. Based on the outer membrane anchoring elements of V. anguillarum , we have previously constructed several efficient surface display systems Lpp-Omporf1, Lpp-OmpU, Lpp-Omp26La, Wza-Omporf1, Wza-OmpU and Wza-Omp26La. In this study, with these constructed surface display systems, a putative antigen protein EseB from pathogenic Edwardsiella tarda was successfully expressed on the surface of an attenuated V. anguillarum strain to get multivalent vaccine candidates. Further immune protection evaluation in zebra fish ( Danio rerio ) demonstrated that the V. anguillarum EseB-display strain AV/pW-26La-B could trigger full protection against V. anguillarum infection and early protection against E. tarda infection in the immunized fish. These results suggest that surface display of heterologous protective antigens in attenuated V. anguillarum could be used as a tool to develop potential V. anguillarum vector vaccine. 相似文献
998.
999.
2004年和2005年分别对江苏省沿海滩涂4个具有代表性的文蛤重点增养殖区的环境因子进行了连续调查、监测和研究,主要分析测试了以下3个对文蛤生存影响较为重要的环境因子:底泥中的硫化物(S2-)、底泥渗水中的总氨氮(NH4-N)和底泥渗水中的化学需氧量(COD)。结果表明:S2-季节性变化明显,地点上随监测站位不同有显著差异;NH4-N夏季较高,其它时段基本无变化,均值远远高于上覆水体;COD含量除受气温影响外,还显著受到人类生产生活(如排污等)的影响。另外,2005年较2004年部分监测站位养殖环境有恶化趋势。 相似文献
1000.
4个种群日本沼虾(Macrobrachium nipponensis)于2001年8月分别取自太湖、洪泽湖、广西南宁3地天然水域及浙江德清养殖种群。肌肉营养成分测定结果表明,养殖种群的粗蛋白含量为(15.83±0.32)%(质量分数),显著低于3个野生种群(P<0.05),而粗脂肪质量分数为(1.37±0.02)%显著高于野生种群(P<0.05)。3个野生种群中洪泽湖种群鲜味氨基酸含量最高;但太湖种群粗蛋白含量(17.71±0.38)%最高,必需氨基酸组成最为均衡,且单不饱和脂肪酸总量和多不饱和脂肪酸总量均为最高,分别达25.904%和35.953%。比较得出太湖种群日本沼虾肌肉的营养品质最高,养殖种群最低。 相似文献