猪链球菌2型诊断与防制措施

<正>猪链球菌(Streptococcus suistype 2,SS2)已经成为危害养猪生产及人类健康的重要人畜共患病。可引起猪脑膜炎、关节炎、心内膜炎及猝死等;致人败血症、心内膜炎、脑膜炎,严重的致人链球菌毒素休克综合征而死亡。1998年7月江苏南通某地猪群暴发猪链球菌2型,同时导致21     

电解多维对缓解断奶仔猪转群应激的探讨

目前，规模化养猪场一般采取２８－３５日龄的仔猪早期断奶，在很大程度上提高了母猪的生产力及饲料的利用率，提高了猪场的经济，但随着仔猪断奶日龄的提高，也出现了许多生产上的问题，其中较为普遍而且影响较为严重的就是仔猪断奶后的转群应激。 在生产实践中，我们已克服了仔猪断奶应激，通过半年多的反复对比试验发现，在断奶仔猪转群后的３ｄ－５ｄ天内，饲料中添加一定量的电解多维，对缓解断奶仔猪因转群造成的食欲不振、消化不良、腹泻、膘情下降、抗病力减弱等不良反应均能起到满意的效果。１材料与方法１．１电解多维山东省明发…     

褪黑素和光照对鹌鹑白细胞介素-2的影响

采用褪黑素(MLT)完全抗原主动免疫鹌鹑，在不同光照条件下饲养，研究了MLT参与神经-内分泌-免疫网络调控时对IL-2水平的影响。结果表明：主动免疫后，MLT和IL-2水平明显下降；随着光照时间的延长，IL-2水平也显著下降。一定剂量的MLT可促进机体免疫因子IL-2的分泌。     

发酵床陈化垫料腐解复合微生物菌剂筛选

为了探讨微生物茵剂对发酵床陈化垫料堆肥腐解的影响,以陈化垫料为原料,用实验室分离纯化得到不同类型的微生物菌株,选择搭配优势群体组成复合微生物茵剂,进行堆肥腐解试验。结果表明：添加不同配比比例的枯草芽孢杆菌,康宁木霉和高温放线茵能有效促进陈化垫料腐解,其中接种复合微生物茵剂（Kc：Kn：Gf=l：1：1）腐解效果最好,高温保持的时间达13d,堆肥结束时,纤维素降解率、木质素降解率、发芽率指数、C／N分别为47．6％、30．2％、98．5％、18．sN,大肠杆菌未检出。     

如何提高缉毒犬的实际工作能力

缉毒犬对各种毒品气味的分辨能力,即一头受训犬能够在各种复杂的环境中,准确地从烟、酒、食物、糖、茶、化妆品、药材以及化学物品等干扰气味中分辨出某种毒品气味,并反应正确。目前,有些地方的缉毒犬只是对单一毒品气味进行了训练：并且考虑在更为复杂的环境下的训练,即模拟训练。这种训练接近实战,要求带犬民警充分考虑到实战现场的复杂性,并以此为标准,对模拟训练中的三种形式进行合理的布局。     

手术治疗真胃移位应注意的问题

随着奶牛单产水平的不断提高，真胃移位病例时有发生，治疗该病已是每个兽医工作者必备的技能。治疗有多种方法：禁食、泻下、健胃法和手术法。前两种方法治疗复发率比较高，后者疗效确切。笔者治疗该病采用手术疗法，在多数病例中总结出了手术法治疗该病值得注意的几个问题，写出来供同行参考。     

L-arginine prevents reduced expression of endothelial nitric oxide synthase (NOS) in pulmonary arterioles of broilers exposed to cool temperatures

This study investigated nitric oxide synthase (NOS) expression in the endothelium of pulmonary arterioles of broilers during the development of pulmonary hypertension syndrome (PHS). PHS was triggered by exposing broilers to sub-thermoneutral (cool) temperatures and an additional 1.0% L-arginine was added to the basal diet to evaluate the effects of supplemental L-arginine on nitric oxide (NO) production, endothelial NOS expression, and the incidence of PHS. Cumulative mortality from PHS, right/total ventricle weight ratios (RV/TV), and body weights were recorded. Plasma NO concentration and NOS expression in the endothelium of pulmonary arterioles with an outer diameter ranging from 100 to 200 microm were determined. Birds exposed to cool temperatures had increased pulmonary hypertension and PHS mortality and diminished endothelial NOS expression. Supplemental dietary L-arginine reduced PHS mortality and elicited higher NOS expression within the pulmonary endothelium coincident with elevated NO production. The results demonstrated that broilers developing PHS exhibited diminished NOS expression in the endothelium of their pulmonary arterioles. Supplemental L-arginine prevented the reduced expression of NOS in the pulmonary endothelium, which might contribute to the increased production of NO by the pulmonary vasculature.     

Inhibition of porcine circovirus type 1 and type 2 production in PK-15 cells by small interfering RNAs targeting the Rep gene

Porcine circovirus type 1 (PCV1) and type 2 (PCV2) are two genotypes of porcine circovirus. Both of them are presumed to be widespread in the swine population. Currently, there is no specific treatment for their infections. RNA interference (RNAi) is a sequence-specific RNA degradation mechanism mediated by small interfering RNA (siRNA), which represents a possible therapeutic application for the treatment of viral infections. In this study, three siRNA expression plasmids (pS-RepA, pS-RepB and pS-RepC) were generated to target three different coding regions of the Rep protein (Rep) of PCV. These siRNAs were used to inhibit PCV production in a porcine kidney cell line, PK-15 cells. Our results revealed that Rep gene expression was inhibited by pS-RepA, pS-RepB and pS-RepC to different degrees. Moreover, our study also showed that the production of PCV1 and PCV2 was reduced by these siRNAs. pS-RepC, which targets the middle region of Rep gene, proved to be the most efficient siRNA for inhibition of Rep expression and viral production. Taken together, our data suggest that RNAi could be investigated as a potential treatment for PCV infection.     

一起未经检疫调运牲畜引发疫情的调查处理

1案情2003年3月23日锡盟正镶白旗改良站从通辽市科左后旗查金台牧场购入26头西门塔尔牛 (其中3头种公牛 ) ,用汽车从科尔沁区运抵锡盟正镶白旗羊场 ,并与2002年11月从通辽科左后旗查金台牧场购入的10头牛混群饲养。24日下午起 ,新购进牛陆续发病 ,至31日病牛已达26头 (新购和原购的牛都有 ) ,旗兽医站接到疫情报告后即派兽医人员到羊场调查情况 ,并采取了相应措施。兽医人员初步诊断为某一类病 ,于是向旗、盟畜牧局和政府上报疫情。并采取病料送自治区兽医站检验诊断 ,确诊为某一类传染病。2处罚经自治区动物防疫监督检验所调查取证 ,这是一…     

提高"仙湖3号"种鸭生产性能的技术措施

“仙湖3号”鸭属快大、瘦肉型新品种,成活率高,49天龄体重3.0～3.5kg,父母代种鸭具有产蛋率高,受精率高,孵化率高等优点,是“仙湖”鸭课题组培育的一个优良品系。本文简要介绍提高“仙湖3号”种鸭生产性能的技术措施,希望通过改进饲养管理,提高经济效益。