绿脓假单胞菌致死海豚的病理变化

1997年～1998年青岛市海豚表演馆饲养的3只海豚小青、小红、小黑分别于1997年9月、1998年2月、5月因绿脓假单胞菌感染而引起发病. 　　……     

猪传染性胃肠炎诊断与不同治疗方法的疗效观察

本研究针对鱼台县疑似患猪传染性胃肠炎的30～45日龄仔猪,通过临床症状、病理剖检和猪传染性胃肠炎病毒抗体检测ELISA诊断试剂盒确诊为猪传染性胃肠炎。随机抽取四圈57头患猪,按圈分成4组,分别用黄芪多糖-紫锥菊注射液+复方硫酸安普霉素注射液穴位注射、黄藤素注射液+氟苯尼考注射液、黄芪多糖注射液+烟酸诺氟沙星溶液、利巴韦林注射液+硫酸庆大霉素注射液治疗。试验结果表明:黄芪多糖-紫锥菊注射液+复方硫酸安普霉素注射液穴位注射的组方在该地区的治疗效果最好,其次是黄藤素注射液+氟苯尼考注射液的组方、黄芪多糖注射液+烟酸诺氟沙星溶液组方,利巴韦林注射液+硫酸庆大霉素注射液的组方的效果最差。     

Immune responses of orange-spotted grouper,Epinephelus coioides,against virus-like particles of betanodavirus produced in Escherichia coli

Betanodaviruses are the causative agents of viral nervous necrosis (VNN), a serious disease of cultured marine fish worldwide. Virus-like particles (VLPs) are one of the good novel vaccine candidates to control this disease. Until now, betanodavirus vaccine studies mainly focused on the humoral immune response and mortality after virus challenge. However, little is known about the activation of genes responsible for cellular and innate immunity by vaccines. In the present study, VLPs of orange-spotted grouper nervous necrosis virus (OGNNV) were produced in prokaryotes and their ability to enter Asian sea bass cells was the same as native virus, suggesting that they possess a similar structure to OGNNV. VLPs immunogenicity was then determined by intramuscularly vaccinating Epinephelus coioides at different concentrations (1.5 or 15 μg g^?1 fish body weight, FBW) and immunizing frequencies (administration once, twice and thrice). A single vaccination with the dosage of 1.5 μg g^?1 FBW is enough to provoke high titer antibodies (average 3 fold higher than that of negative control) with strong neutralizing antibody titer as early as 1 week post immunization. Furthermore, quantitative PCR analysis revealed that eleven genes associated with humoral, cellular and innate immunities were up-regulated in the liver, spleen and head kidney at 12 h post immunization, correlating with the early antibody response. In conclusion, we demonstrated that VLP vaccination induced humoral immune responses and activated genes associated with cellular and innate immunity against betanodavirus infection in orange-spotted grouper.     

Leucine markedly regulates pancreatic exocrine secretion in goats

Four goats (30.1 ± 1.3 kg) with common bile duct re‐entrant catheter and duodenal catheter were used to evaluate the effects of duodenal leucine infusion on pancreatic exocrine secretion and plasma parameters with two 4 × 4 Latin square design experiments. In the long‐term infusion experiment, goats were fed twice daily [700 g/day, dry matter (DM) basis] at 8:00 and 18:00 hours and were duodenally infused with 0, 3, 6, 9 g/day leucine for 14 days. Pancreatic juice and jugular blood samples were collected over 1‐h intervals for 6 h daily from d 11 to 14 days to encompass a 24‐h day. In the short‐term experiment, goats were infused leucine for 10 h continuously at the same infusion rate with Experiment 1 after feed deprivation for 24 h repeated every 10 days. Pancreatic juice and blood samples were collected at 0, 1, 2, 4, 6, 8 and 10 h of infusion. The results showed that the long‐term leucine infusion did not affect pancreatic juice secretion, protein output, trypsin and lipase secretion and plasma insulin concentration, but linearly increased α‐amylase secretion. No changes in pancreatic protein and lipase secretion were observed in the short‐term infusion. Pancreatic juice and α‐amylase secretion responded quadratically, with the greatest values observed in the 3 and 6 g/day leucine respectively. Trypsin secretion linearly decreased, while plasma insulin concentration increased linearly with increased leucine infusion. The results demonstrated that duodenal leucine infusion dose and time dependently regulated pancreatic enzyme secretion not associated with the change in plasma insulin concentration.     

Identification of lactic acid bacteria in the feces of dairy cows fed whole crop maize silage to assess the survival of silage bacteria in the gut

In order to assess the survival of lactic acid bacteria (LAB) in whole crop maize silage in the gut of dairy cows, one representative silage sample and three different feces samples were collected from dairy cows on three dairy farms in Hua Bei, China and three dairy farms in Kyushu, Japan. The composition of the bacterial community was examined by denaturing gradient gel electrophoresis and quantitative polymerase chain reaction. Lactobacillus acetotolerans was detected in all bunker‐made maize silage samples, regardless of the dairy farm or sampling region from which they were sourced. A total of eight LAB species were detected in the maize silage samples, of which three (L. acetotolerans, L. pontis and L. casei) appeared to survive digestion. The populations of L. acetotolerans in silage and feces were 10^6–7 and 10^3–4 copies/g, respectively, indicating that, even for the LAB species showing potential survival in the gut, competition in this niche may be harsh and the population may substantially decrease during the digestion process. It may be difficult for silage LAB to survive in the gut of silage‐fed dairy cows, because marked decrease in population can take place during the digestion process, even for surviving species.     

中国荷斯坦牛初产日龄遗传评估及全基因组关联分析

基于高通量SNPs测序的全基因组关联分析为奶牛繁殖性状相关基因的探索提供了契机。本研究基于课题组前期中国荷斯坦牛基因组测序芯片的结果,通过DMU(v 6.0)采用AI-REML结合EM算法的动物模型对北京地区2001-2011年10年间19 111头中国荷斯坦母牛初产日龄记录进行遗传参数估计,并应用PLINK(v 1.07)将大群估计的2 172头中国荷斯坦母牛初产日龄性状的育种值(EBV)作为表型值,进行基于无关群体设计的全基因组关联分析。通过全基因组水平的Bonferroni校正,共检测到1 700个SNPs与初产日龄显著相关。选取P值达到10-15的43个SNPs进行初步分析,其中12个位于X染色体上。显著SNPs上下游200kb范围内存在KLHL4、TRAM1、TRAM2、ZNF438、MATK等繁殖障碍疾病相关基因。7号染色体1 Mb(20.42~21.52 Mb)区域内多个SNPs位点与初产日龄显著相关。这些基因和区域可以作为影响初产日龄性状的重要候选基因和区域,为揭示奶牛繁殖性状的分子遗传基础积累了素材。     

饲粮锌和钙水平对产蛋鸡生产性能、血液生化指标和组织中锌含量的影响

试验在玉米-豆饼型基础饲粮中(含锌29.2ppm)添加不同水平锌和钙研究了饲粮锌和钙水平对蛋用型鸡产蛋期生产性能、血液生化指标和组织中锌含量的影响。结果表明:1.仅喂未加锌的基础饲粮的试验鸡开产晚(P<0.05),产蛋率低(P<0.05)、蛋重小(P<0.05)、产蛋量少(P<0.01)、饲料利用效率差(P<0.01)、破壳率高(P<0.01)。饲粮加锌1000ppm与加锌40ppm对照相比,生产性能无显著差异。饲粮高钙对产蛋鸡生产性能无不良影响。2.血清碱性磷酸酶活性随饲粮加锌水平的提高而显著提高(P<0.01),但不受饲粮钙水平显著影响(P>0.05)。饲粮加锌1000ppm使血清总蛋白含量(P<0.01)、血清白蛋白和球蛋白含量(P<0.05)显著降低。饲粮高钙使血清总蛋白含量(P<0.01)和白蛋白含量(P<0.05)显著降低,而球蛋白含量不受影响(P>0.05)。3.随饲粮加锌水平的提高,肝脏(P<0.01)、胰脏(P<0.01)和胫骨(P<0.01)中锌含量大幅度升高;血液(P<0.01)、肾脏(P<0.01)和肺脏(P<0.05)中锌含量升高幅度较小;而输卵管(P>0.05)、心脏(P>0.05)和肌肉(P>0.05)中锌含量无显著变化。饲粮高钙使肝脏(P<0.05)和肌肉(P<0.01)中锌含量显著降低,对其它器官无显著影响(P>0.05)。     

家蚕消化液对几种外源性酶活性的影响

通过体外试验和经口添食试验表明,家蚕消化液对纤维素酶和果胶酶具有明显的 抑制作用.分析认为,这是由于消化液的强碱性或消化液中存在的蛋白酶的分解作用 所致.因此,利用外源性消化酶的可能途径是先经酶促反应,将大量纤维素或果胶等 转变成可吸收利用的还原糖形态,然后进行给饵.     

人工践踏对无土草坪基质的影响

本试验采用人工践踏的方式,研究了不同组合配比的基质对无土草坪耐践踏性的影响。结果表明,良好的无土草坪基质配比及适合的厚度是保证无土草坪优良的耐践踏性及观赏性的关键。本试验中,草坪草生长基质层和弹性材料层均为25 mm,结构合理、厚度适中,克服了现有草坪基质薄、持水力差的缺点。在相同践踏强度下,能维持较高水平的草层高度、草坪盖度和草坪草分蘖数,分别达到了5.14 cm、97.60%和123.97 个·dm-2,且停止践踏后草坪质量能快速恢复。均匀混合的草坪草生长基质层和弹性材料层,在厚度一致的前提下并不耐践踏,实际效果不好。土壤坪床的处理效果最差。因此,具有独立的草坪草生长基质层和弹性材料层且厚度适中的基质,适用于生产耐践踏性好的无土草坪。     

牛血清中口蹄疫病毒非结构蛋白抗体时间分辨荧光免疫分析检测方法的建立

为研究快速定量检测牛血清中口蹄疫病毒非结构蛋白抗体的方法,试验通过优化抗原表达条件等步骤,在大肠杆菌原核表达系统中表达可溶性的3A-3B融合蛋白,并基于纯化的可溶性融合蛋白建立口蹄疫病毒非结构蛋白抗体时间分辨荧光免疫分析检测试剂盒。结果表明:建立的方法能够检测牛血清中的口蹄疫病毒非结构蛋白抗体,敏感性高,特异性强,对其他相关的牛类病原无交叉反应,其组内与组间变异系数分别低于10%和15%,具有良好的重复性。对300份临床牛血清样品进行检测,同Procheck公司的口蹄疫非结构蛋白抗体试剂盒进行比较,阳性样品符合率96%,阴性样品符合率93.3%,总的符合率95.7%。重复性试验组内与组间变异系数均小于10%。文章首次建立了口蹄疫病毒非结构蛋白抗体时间分辨荧光免疫分析检测方法,同传统的ELISA方法相比,该检测方法特异性相当、敏感性更高,操作更简单、快速,具有较高的应用推广价值。