抗体夹心BGT-ELISA方法的建立及在转基因白桦中的应用

以蜘蛛杀虫肽与Bt-toxinC肽融合蛋白基因(bgt基因)的表达产物的定量分析为研究目标,采用原核表达及纯化出融合蛋白His-BGT作为抗原进行兔免疫,得到相应的抗血清,采用ELISA法检测效价在1:10000以上,并对抗血清进行了免疫亲和层析,获得了高纯度的IgG,Westernblot检测具有较好的特异性。采用过碘酸钠法将抗体标记辣根过氧化物酶(HRP),得到第二抗体即酶标抗体,标记率在85%以上。建立起检测BGT杀虫蛋白的快速、灵敏的方法。应用该检测方法,分析了不同转基因白桦(BetulaplatyphyllaSuk.)株系中BGT蛋白含量占叶片可溶性总蛋白含量的0.05%～0.3%,并利用Westernblot验证了此方法是可靠的。说明抗体夹心BGT-ELISA方法能够定量分析转基因植株中BGT蛋白的含量,为转bgt基因植物的检测和应用奠定了基础。     

Stabilization Properties of Aged refuse in Semi aerobic and Anaerobic Landfills

The stabilization properties of aged refuse in five year old semi aerobic and anaerobic landfills were compared, such as volatile solid (VS), total organic carbon content (TOC), humic matters, and so on. It was found that VS and TOC content in aged refuse were obviously lower than those in fresh refuse after five years disposal. However, the VS and TOC content of aged refuse in semi aerobic landfill were slightly lower than anaerobic ones, whilst the difference was not significant. Surface settlements of semi aerobic landfills were higher than that of anaerobic ones (P<0.05). Moreover, there was negative correlation between VS content and surface settlements. Both leachate and water recycling methods did not lead to significant VS content difference, while leachate recycling would cause obviously uneven surface settlement. Biological degradable materials (BDM) value of aged refuse in semi aerobic landfill and anaerobic one was similar. Furthermore, BDM value increased with the increase of air pipe distance and depth. The greater the air pipe distance is, the bigger the difference of BDM value between the layers is. In addition, total extraction ratios of humic matters and HA/FA in semi aerobic and anaerobic landfill aged refuse had no significant differences.     

Design method of generatingDICOM Image in the high frequency X ray machine

DICOM3.0 standard is complex and rich in contents. Aiming at the difficulty of software design, a programming method of generating DICOM image file in the high frequency X ray machine is proposed. The data structure of DICOM file is analyzed, and then the programming method of generating DICOM image files is introduced in detail. For the proposed method, a template array of the data structure is constructed to save the eigenvalues, transfer syntax, pixel data and so on of the image. All kinds of the data are stored in the DICOM file successively. The programming method can not only transform a BMP file into a DICOM file, but also transform a JPEG file into a DICOM file. It strictly follows DICOM 3.0 protocol. The generated DICOM files can be displayed with the DICOM file browsing software ezDICOM. The programming design was based on VC++6.0.     

杂草对光系统Ⅱ抑制剂的抗药性研究进展

随着除草剂使用量和使用频率的不断增加,杂草抗药性问题也逐渐成为杂草防除及治理的难点和热点.目前杂草对PS(Photosystem)Ⅱ抑制剂类除草剂产生抗药性主要分为靶标抗性和非靶标抗性.本研究综述了近年来杂草对PS Ⅱ抑制剂类除草剂抗药性的产生、发展现状及杂草抗PS Ⅱ抑制剂类除草剂的机理.针对这些问题,提出除草剂使用...     

柑橘皮渣降解酶的筛选

从6种果胶酶中,筛选出对柑橘皮渣降解作用良好的果胶酶W1,适宜用量为0.05%;从5种纤维素酶中,筛选出对柑橘皮渣降解作用良好的纤维素酶R-10,适宜用量为0.01%。由果胶酶W1和纤维素酶R-10组成的复合酶,适宜的酶解温度为30℃,酶解时间为24h。     

草地贪夜蛾寄主选择性及对其生长发育的影响

旨在为草地贪夜蛾的预测预报、科学防控、适生性分析以及风险评估提供参考依据。以盆栽香蕉苗、玉米苗和草地贪夜蛾3~4龄幼虫作为试材,用室内接虫和田间自然感虫的方法,测定了选择压力和非选择压力条件下草地贪夜蛾对香蕉和玉米的危害指数、草地贪夜蛾发育历期、幼虫存活率、蛹体大小及成虫羽化率。在非选择压力条件下草地贪夜蛾主要选择危害玉米,对香蕉‘桂蕉6号’只是试探取食,接虫7天后对玉米和香蕉危害指数分别是89.05、1.58。在选择压力下草地贪夜蛾幼虫接虫3天后开始取食危害香蕉,接虫后3、5、7天危害指数分别为11.74、16.51、24.76,说明香蕉不是草地贪夜蛾适宜的寄主作物。取食香蕉的草地贪夜蛾幼虫生长缓慢、虫体小,3龄幼虫至化蛹历时9~14天,比取食玉米的草地贪夜蛾长2~4天;成虫羽化历时9~14天,比取食玉米的草地贪夜蛾长4~7天;取食香蕉明显影响草地贪夜蛾生长发育,幼虫存活率为20.0%、化蛹率68.1%、蛹体大小1.25×0.35 cm、蛹重0.13 g、成虫羽化率47.8%,明显低于取食玉米寄主的幼虫存活率为86.7%、化蛹率97.4%、蛹体大小1.57×0.5 cm、蛹重0.21 g、成虫羽化率92.0%。草地贪夜蛾在选择压力下会取食危害香蕉,危害程度相对较轻,能完成世代生活史,但生长发育速度也较慢。香蕉不是草地贪夜蛾适宜的寄主植物,但在香蕉上暴发存在一定的风险。     

化州市化橘红种质资源主成分分析和聚类分析

应用主成分分析和聚类分析,从宏观和微观2个层面分析鉴别化橘红种质资源,以期为化橘红种质资源的鉴定、评价、保护、开发和利用提供参考依据.利用SPSS 19.0对化橘红种质资源17项主要植物学数量性状指标和6项理化指标进行变异性和主成分分析,采用MEGA7对SNP结果进行聚类分析.研究发现,不同化橘红种质资源在有效成分、花...     

种苗处理对麦冬生长的影响及其控病效果研究

为了明确种苗处理对麦冬生长的影响及其控病效果,指导麦冬优质安全生产.以川麦冬为供试材料,采用田间小区试验,研究了 6种药剂3种种苗处理方式对麦冬种苗成活、生长和产量的影响,以及对炭疽病、根腐病的控病效果.结果表明木霉T23制剂、多抗霉素和噁霉灵处理种苗,可显著提高麦冬种苗成活率,促进麦冬分蘖、增加块根数、使块根伸长增粗...     

硅肥对水稻产量、品质及抗倒伏特性的影响

研究不同硅肥对宁化河龙贡米产量、品质和抗倒伏性状的影响,以期为宁化河龙贡米的可持续稳定发展提供技术支撑.以'玉针香'为试验材料,设置3个不同硅肥处理[森夫硅肥(S1)、蔡德龙ETDA硅肥(S2)、不施硅肥(S0,对照)],调查不同硅肥处理对'玉针香'产量形成、品质及茎秆形态和力学特征相关性状的影响.结果表明,施用S1处...     

油茶FAD2基因全长cDNA的克隆和序列分析

FAD2基因控制油酸转化为亚油酸,是油茶油脂合成代谢的关键酶基因.在构建的油茶.EST文库基础上,采用5'RACE和交错延伸PCR技术获得了油茶FAD2基因的全长cDNA克隆.该基因序列长1 682 bp,开放阅读框为1 149 bp,编码382个氨基酸,并且具有FAD2特有的保守序列和相关特征.经过比对分析,发现油茶的FAD2基因与其他植物的FAD2基因具有较高的相似性.