乌桕金带蛾新种记述(鳞翅目:带蛾科)

本文为贵州省的重要林木害虫－乌桕金带蛾的新种记述，与近缘种中华金带蛾详加比较，附有特征图以兹鉴别。     

Anal leiomyoma in a Holstein heifer.

A 2-year-old heifer was presented with masses on her anus that were interfering with cervical manipulation during embryo flushing. The masses had broad stalks attached within the anal sphinchter. Recovery was without incident after surgical resection. No recurrence of the masses had occurred 3 months later. Histologic diagnosis was benign leiomyoma.     

南方山区草地资源的特点及开发利用途径和原则

根据生态学的基本原理，对我国南方山区草地资源的特点进行了分析，从生成学类型上该地区划时进行了划分，进而提出了南方山区草地开发利用的途径和原则。     

牛乳免疫球蛋白和补体含量测定

用琼脂单项免疫扩散法测定牛乳免疫球蛋白和补体含量。结果表明：初乳免疫球蛋白和补体Ｃ３含量极显著高于常乳（Ｐ〈０．０１）;初乳补体Ｃ４含量显著高于常乳（Ｐ〈０．０５）;初乳中免疫球蛋白和补体含量于产后３天达到高峰。     

哺乳动物腔前卵泡研究进展及其在珍稀濒危野生动物上的应用

回顾哺乳动物(牛、羊、猪、小鼠、大鼠、兔以及仓鼠等)腔前卵泡的研究历史和现状并探讨腔前卵泡研究在珍稀濒危野生动物(猫科动物、犬科动物和灵长类动物等)上应用的可能性.     

鹦鹉热衣原体荧光环介导等温扩增（LAMP）检测方法的建立

鹦鹉热衣原体(Chlamydia psittaci)是一种人兽共患病原体,能引起自然疫源性衣原体病,目前广泛分布于世界各地。本研究应用环介导等温扩增技术(LAMP),针对鹦鹉热衣原体23S RNA基因序列设计引物,并进行筛选以及敏感性和特异性试验,建立了鹦鹉热衣原体恒温荧光扩增方法。该方法在63℃恒温条件下1 h内即可显示结果,操作简单、快速,特异性强,灵敏度可达100拷贝/μL,且与流产衣原体、动物布鲁氏菌、牛结核分枝杆菌、沙门氏菌、大肠杆菌、金黄色葡萄球菌无交叉反应;用国产仪器可直接通过检测荧光信号判读结果,既增强了准确性,也避免了开盖污染产生假阳性;应用建立的LAMP方法对实验室保存的临床DNA样品进行检测,发现检测结果与QPCR相同。该方法的建立弥补了传统检测技术的不足,为实现鹦鹉热衣原体的现场快速诊断提供了技术支持。     

牛支原体和牛病毒性腹泻病毒二重二温式PCR检测方法的建立

为建立一种牛支原体(Mycoplasma bovis,MB)和牛病毒性腹泻病毒(Bovine viral diarrhea virus,BVDV)的快速鉴别诊断方法,针对MB的uvr C基因和BVDV的5'端非编码区(5'-UTR)保守基因序列,分别设计两对特异引物,并将三温式PCR扩增程序简化为二个温度梯度,建立了鉴别MB和BVDV的二重二温式PCR方法。该方法能同时扩增MB和BVDV,扩增产物大小分别为412和170 bp。特异性试验结果显示,该方法对参试的所有毒株只扩增MB和BVDV基因组,对其它牛病原体无扩增;敏感性试验结果显示,该方法最低能同时检测到104拷贝的两种目的核酸;干扰性试验结果显示,该方法能同时检测两个模板不同浓度的组合,试验结果不受模板影响。综上,本研究所建立的二重二温式PCR方法特异、敏感、快速、简便,可应用于MB和BVDV临床鉴别诊断和流行病学调查。     

Pharmacokinetics of sanguinarine,chelerythrine, and their metabolites in broiler chickens following oral and intravenous administration

Sanguinarine (SA) and chelerythrine (CHE) are the main active components of the phytogenic livestock feed additive, Sangrovit®. However, little information is available on the pharmacokinetics of Sangrovit® in poultry. The goal of this work was to study the pharmacokinetics of SA, CHE, and their metabolites, dihydrosanguinarine (DHSA) and dihydrochelerythrine (DHCHE), in 10 healthy female broiler chickens following oral (p.o.) administration of Sangrovit® and intravenous (i.v.) administration of a mixture of SA and CHE. The plasma samples were processed using two different simple protein precipitation methods because the parent drugs and metabolites are stable under different pH conditions. The absorption and metabolism of SA following p.o. administration were fast, with half‐life (t_1/2) values of 1.05 ± 0.18 hr and 0.83 ± 0.10 hr for SA and DHSA, respectively. The maximum concentration (C_max) of DHSA (2.49 ± 1.4 μg/L) was higher that of SA (1.89 ± 0.8 μg/L). The area under the concentration vs. time curve (AUC) values for SA and DHSA were 9.92 ± 5.4 and 6.08 ± 3.49 ng/ml hr, respectively. Following i.v. administration, the clearance (CL) of SA was 6.79 ± 0.63 (L·h^?1·kg^?1) with a t_1/2 of 0.34 ± 0.13 hr. The AUC values for DHSA and DHCHE were 7.48 ± 1.05 and 0.52 ± 0.09 (ng/ml hr), respectively. These data suggested that Sangrovit® had low absorption and bioavailability in broiler chickens. The work reported here provides useful information on the pharmacokinetic behavior of Sangrovit® after p.o. and i.v. administration in broiler chickens, which is important for the evaluation of its use in poultry.     

藏山羊乳主要营养成分及乳酶、乳清蛋白的研究

本文研究了分布于西藏和四川地区的藏山羊乳蛋白、乳糖和乳脂含量,并对乳中4种酶活力及乳清蛋白的组成进行了研究。结果表明:藏山羊乳中3种营养成分存在一定的地区差异,脱脂乳中乳酸脱氢酶,碱性磷酸酶,γ-谷氨酰转肽酶、乳过氧化物酶活力分别为81.9±37.8、7.7±3.5、437.4±93.0、532.7±202.6单位。乳过氧化物酶在体外可被10~(-5)M的硫氰酸根或乳酸根活化。用琼脂糖凝胶电泳可将乳LDH分出LDH_1、LDH_2和LDH_3三带,各带所占百分比例分别为50.6±4.5、31.6±4.2和17.8±3.3,提示乳LDH不是来源于血液。用SDS—PAGE可将乳清蛋白分出5条区带。     

白色力克斯兔、哈尔滨白兔及杂交后代血液蛋白多态性研究Ⅱ.用血液蛋白多态性剖析亲本品种与杂种后代的遗传关系

根据所测定的白色力克斯兔(WR)、哈尔滨白兔(HW)及杂种一代(F_1,?WR×?HW)和回交一代[B_1,?WRX×?F_1,包括分离出的力克斯毛型(B_1R)和正常毛型(B_1N)]的5个血液蛋白多态系统——红细胞酯酶(Es—1、Es—2和Es—3)、前转铁蛋白(Prt)和后白蛋白(Po)的基因频率和基因型新率,比较了群体间的基因分布,估算了各群体内的平均杂合度和群体间的遗传距离,并运用类平均聚类法进行了聚类分析.结果表明,白色力克斯兔和哈尔滨白兔基因分布差异最大.亲缘关系最远;杂种一代和回交一代基因分布差异最小,亲缘关系最近;杂交一代和回交一代与白色力克斯兔亲缘关系较近,与哈尔滨白兔较远;回交一代中B_1N和B_1R间基因分布也存在一定差异.各群体平均杂合度大小为0.5223(HW)、0.4182[B_1,0.4846(B_1N)和0.3223(B_R)]、0.1105(F_1)和0.3946(WR).研究结果表明,利用蛋白多态性探讨家兔品种或品系间亲缘关系以及区分家兔品种或品系是可行的.此外,本文还结合各群体的变异性,对白色力克斯免和哈尔滨白兔的选育进行了探讨.