Experimental research on cardiac fibrosis induced by recombinant mouse interleukin-11 in mice

AIM To observe the effect of recombinant mouse interleukin-11 （rmIL-11）injected subcutaneously into mice on heart structure and function and to determine its pro-fibrotic effect. METHODS C57BL/6 mice were randomly divided into experimental group and control group.The mice in experimental group were injected subcutaneously with recombinant mouse IL-11 at the dose of 100 μg·kg^-1·d^-1 for 3 consecutive weeks, while the control group were given equal volume of normal saline in the same way. After the experiment was finished, the parameters of heart function were measured by echocardiography.The heart weight was weighed and the cardiac weight index (CWI) was calculated. HE staining and Masson's trichrome staining were performed to observe the pathological changes and the extent of myocardial fibrosis in mouse myocardia respectively, and the cardiac collagen volume fraction (CVF) was calculated. The expression levels of extracellular matrix proteins in the myocardial tissues of mice, including type Ⅰ collagen, type Ⅲ collagen and fibronectin, were determined by Western blot. RESULTS Left ventricular ejection fraction and left ventricular fraction shortening in experimental group were obviously lower than those in control group (P<0.01), however left ventricular end-diastolic diamension and left ventricular end systolic dimension were significantly higher than those in control group (P<0.05).Compared with control group, the CWI was increased (P<0.01), the myocardial arrangement was disorder, the necrosis of cardiac myocytes was increased, and excessive deposition of collagen was observed in the myocardial tissues in experimental group. Correspondingly, the CVF and protein levels of type Ⅰ collagen, type Ⅲ collagen and fibronectin in the left ventricle in experimental group were increased significantly (P<0.05). CONCLUSION Injection of rmIL-11 into the mice subcutaneously induces fibrogenesis in the heart, which implies that IL-11 is likely a novel pro-fibrotic factor.     

GA3介导miR171s及其靶基因VvSCLs调控葡萄种子发育的作用分析

[目的]鉴定vvi-miR171s成员及其靶基因,明确vvi-miR171s成员及其靶基因在葡萄种子发育中的重要作用及其响应赤霉素(Gibberellin,GA)调控种子发育的表达模式.[方法]以'魏可'葡萄('Wink')果实为试材,利用miR-RACE技术鉴定vvi-miR171a/b/e/f/g/h的成熟体序列;...     

农业区位理论与西部退耕还林区农业产业布局研究

西部是我国重要的生态屏障和重要河流的源头,又是我国重要的农业区,如何在实施退耕还林过程中,兼顾生态效益和经济效益协调、可持续发展,是该项工程能否成功的关键。本文按照农业区位理论和产业布局演变的一般规律,在分析西部退耕还林区现状及农业产业发展趋势的基础上,对西部退耕还林区农业产业布局进行科学规划,并用“吴旗模式”验证其可行性,为西部区域经济发展提供现实的指导意义。     

利用SNP芯片对部分黄淮海玉米自交系遗传多样性分析

利用4 434个SNP标记对47份玉米自交系进行种质遗传多样性和杂种优势类群研究。采用Admixture软件对自交系的遗传结构进行分析,采用Treebest软件对自交系进行聚类分析和类群划分。结果表明,47份自交系分为6个类群,分别为瑞德群、兰卡斯特群、塘四平头群、PB群、旅大红骨群和自330亚群。10个自选系组配的杂交种主要利用的杂种优势模式为PB×塘四平头和瑞德×塘四平头,均为近年来黄淮海地区主要利用的杂种优势模式。     

锌冶炼厂周围农作物和蔬菜的汞污染及健康风险评价

采用对应采样方法研究了葫芦岛锌冶炼厂周围地区3种农作物、20多种蔬菜及其根部土壤的含汞量。结果表明,蔬菜可食用部分受到汞的严重污染,汞含量范围为0．001—0．147mg·kg^-1（干重）;37．1％的叶菜类蔬菜、9％的瓜类蔬菜和4％的豆类蔬菜超过国家食品污染物限量标准;玉米、大豆、高粱中汞污染明显,3者的种子中汞平均含量分别为0．008、006、0．057mg·kg^-1;作物和蔬菜植株中汞含量顺序为叶〉根〉茎〉果实。相关性分析表明,植株根部的汞主要来自于土壤,而叶中的汞则可能来源于大气。通过消费蔬菜．成人汞的平均摄入量为0．015μg·kg^-1·d^-1,最高摄入量为0．051μg·kg^-1·d^-1,儿童汞的平均摄入量为0．020μg·kg^-1·d^-1,最高摄入量为0．069μg·kg^-1·d^-1,仅通过这一途径摄入的总汞平均值就分别占PTWI的2．1％、2．8％,最高值占7．1％、9．7％。     

基于双列杂交的花生主要品质性状遗传效应分析

为了提高花生品质性状优势组合的选育效率,以5个花生材料为亲本,采用Griffing完全双列杂交配制20个组合,分析花生籽仁中粗脂肪含量、粗蛋白含量、蔗糖含量和脂肪酸组分等10个性状的配合力和遗传参数.配合力效应分析表明,豫花132(W191)的粗脂肪、硬脂酸、亚油酸、花生酸和山嵛酸含量等性状的一般配合力效应值最大,冀花...     

机器视觉技术在温室营养液调控系统中的应用

现代设施农业趋向于采用无土栽培.无土栽培是一种采用灌溉营养液来栽培作物的方法.为此,介绍了国内温室无土栽培中营养液供给系统的研究现状,针对目前营养液供给控制存在的问题,将机器视觉技术应用到温室营养液调控中,利用作物的图像信息和环境信息,参考作物专家系统,提出控制策略,调整营养液配置参数,并阐述了系统的软硬件实现方法.     

武威某奶牛场犊牛及职工球虫病感染情况调查

为摸清该奶牛场的球虫感染情况,在甘肃省武威市某集约化牛场随机抽10头犊牛粪便及与犊牛直接接触的一线职工10人的粪便为样本,采用麦克马斯特计数方法,检测球虫病的感染情况。结果显示：该牛场球虫总感染率为80%,但OPG均小于5000,属于亚临床型球虫感染,该奶牛场职工没有球虫病感染情况。通过对球虫病感染的检查,诣在为该场犊牛球虫病的防控治疗提供依据。     

杨木低聚木糖对肉鸡生长性能、肠道消化酶活性和短链脂肪酸含量及血清激素水平的影响

本试验以肉鸡为研究对象,对以杨木屑为原料、采用醋酸催化法制取的低聚木糖进行评价,研究低聚木糖对肉鸡生长性能、肠道消化酶活性和短链脂肪酸含量及血清激素水平的影响,探讨低聚木糖的促生长机制,为醋酸催化法生产低聚木糖的应用提供理论依据.选用120只1日龄爱拔益加肉公鸡,随机分为2个组,即对照组和低聚木糖组,每组6个重复,每个...     

推广BMFB-4型高效免耕施肥播种机——提高保护性耕作机械化水平

我国农业生产环境脆弱,水资源紧缺,现有耕地94970khm^2,旱区约占60％,主要分布在日照长、温差大、富有农业生产潜力的北方和中西部地区。传统的耕作方式使土壤由于过度耕作而造成地表裸露,耕地退化,生态环境恶化。特别是近年来北方尘暴、沙化愈来愈烈,极大制约了农业的发展。迫切需要大力推广应用保护性耕作技术。对农田实行免耕少耕、残茬覆盖、生物覆盖、尽可能减少土壤耕作,减少土壤风蚀、水蚀,提高土壤肥力和抗旱能力。