玉米叶片保绿度F1杂种优势与环境互作效应分析

采用加性-显性及与环境互作的遗传模型,利用玉米6个亲本,按完全双列杂交设计组配的15个杂交组合在4种环境条件下的试验资料,分析玉米叶片保绿度F1杂种优势与环境互作效应。结果表明:玉米叶片保绿度F1杂种优势存在着极显著的基因型与环境互作效应。不同杂交组合在同一环境下的F1杂种优势表现不尽相同,同一杂交组合在不同环境条件下表现也不尽相同。玉米自交系沈137是选育保绿性玉米杂交种较好的亲本材料。     

Effect of poly(ADP-ribose) polymerase on the response of guinea pig tracheas induced by peroxynitrite in vitro

AIM and METHODS: to elucidater the effect of poly(ADP-ribose) polymerase(PARP) on tracheal hyperreactivity of guinea - pig induced by peroxynitrite, the responses of guinea pig tracheas to histamine af- ter incubation with peroxynitrite in the absence and presence of 3 - aminobenzamide(3 - AB), a highly selective inhibitor for PARP, were observed in vitro. RESULTS: The exposure of tracheal strips to peroxynitrite led to epithelial damage and hyperreacitivity to histamine, both of which were reversed by 3 - AB(lmmol/L or 5mmol/L), whereas incubation of tracheal strips with 3 - AB(5mmol/L) had no effect on the reponses. CONCLUSION: PARP is involved in the epithelial damage and hyperreactivity of guinea - pig tracheas induced by peroxynitrite. The results suggested that inhibition of excessive activation of PARP may represent a novel strategy for the prevention and therapy of airway hyperreactivity in asthma.     

Effects of EBV infection on growth and apoptosis of nasopharyngeal carcinoma cell line

AIM: To explore the effect of EBV infection on growth and apoptosis of nasopharyngeal carcinoma(NPC) cell line.METHODS: NPC cell line CNE1 was directly infected by Epstein Barr virus (EBV). The expression of EBV-latent membrane protein 1 (EBV-LMP1) and bcl-2 were detected by immunohistochemistry method (LSAB). The growth of NPC cells was identified by MTT method. Apoptotic carcinoma cells were detected by flow cytometry analysis and the terminal deoxynucletidyl transferase-medicated dUTP-biotin nick end labeling (TUNEL) methods. RESULTS: EBV-LMP1 was positive in CNE1 infected by EBV(E-CNE1). Compared with CEN1, the growth of E-CNE1 apparently increased (P＜0.01). No apoptotic carcinoma cells were detected and bcl-2 postive cells were 2%~3% respectively in 2 kinds of NPC cells.CONCLUSION: Growth of NPC cells is enhanced by EBV infection and EBV-LMP1 expression, but no influence on expression of bcl-2 and apoptosis of NPC cells.     

CYP450 epoxygenase/EET pathway attenuates adipose inflammation of obese mice through HIF-1α

AIM To investigate the effects of cytochrome P450 （CYP450） epoxygenase/epoxyeicosatrienoic acid （EET） pathway on insulin resistance in obese mice, and to explore the possible mechanisms. METHODS High-fat diet-induced obesity model was established in C57BL/6Cnc mice, and the obese mice were randomly divided into 3 groups, including obesity group （treated with saline; n=10）, EET group （treated with 11,12-EET; n=10） and EET inhibitor 14,15-epoxyeicosa-5（Z）-enoic acid （EEZE） group （n=10）. Normal C57BL/6Cnc mice （n=10） treated with saline served as control. Protein expression of CYP2J2 （one of CYP450 epoxygenases） and hypoxia-inducible factor-1α （HIF-1α） was measured by Western blot. Vessel-like structure was detected by immunofluorescence staining. The serum levels of insulin, tumor necrosis factor-α （TNF-α）, interleukin （IL）-1β, IL-6 and monocyte chemoattractant protein-1 （MCP-1） were measured by ELISA. RESULTS In obese mice, homeostasis model assessment of insulin resistance （HOMA-IR） values were increased, the protein level of CYP2J2 was reduced, and the protein level of HIF-1α was increased in adipose tissues as compared with the controls （P<0.05）. The serum levels of MCP-1, IL-1β, IL-6 and TNF-α were also significantly increased in obese mice （P<0.05）. After treatment with 11, 12-EET, the HOMA-IR values were decreased compared with vehicle-treated obese mice, HIF-1α expression levels were decreased in the adipose tissue, and the serum levels of MCP-1, IL-1β, IL-6 and TNF-α were reduced （P<0.05）. Immunohistochemical results of adipose tissue from vehicle-treated obese mice showed a marked decrease in vessel-like structures （CD31-positive） compared with normal control mice （P<0.05）. EET treatment significantly increased the newly formed vessel-like structures in the visceral adipose tissues of obese mice as compared with vehicle-treated obese mice （P<0.05）. CONCLUSION High-fat diet-induced obesity and insulin resistance are closely related to the CYP450 pathway. Exogenous EETs effectively decrease obesity-induced insulin resistance possibly through pro-angiogenesis and attenuation of hypoxia and inflammation.     

Progress in roles of MNX1 antisense RNA 1 in malignant tumor

MNX1 antisense RNA 1 （MNX1-AS1） is a newly discovered long non-coding RNA （lncRNA）, which is highly dysregulated in various carcinomas and its expression level is closely related to the overall survival and prognosis of patients. MNX1-AS1 regulates the occurrence and development of carcinomas by endogenous competitive adsorption of miRNA, regulating cell cycle, inducing epithelial mesenchymal transformation and activating multiple signaling pathways. The in-depth study of the carcinogenesis of MNX1-AS1 is useful for the early diagnosis, targeted therapy and prognostic assessment of relevant carcinomas. This article reviews the roles of MNX1-AS1 in malignant tumor.     

长沙不同绿地对缓解热岛效应的作用

以长沙市学校、公园内的绿地作为研究对象,研究了不同生长发育植物、树冠郁闭度、植物覆盖率对城市热岛效应的影响,得出长势好的植物群落缓解热岛效应的作用大,郁闭度和绿地面积与减缓热岛效应的作用呈正相关。并根据长沙的绿化情况,提出了一些建议。     

玉米小斑病抗性种质鉴定及生理生化分析

以200份玉米优良自交系为材料,2022-2023年夏分别进行小斑病菌的田间接种,综合两年数据共鉴定出高抗小斑病的玉米自交系3份,分别为K22、CML50和齐319;筛选到包括Mo17在内的抗性玉米自交系24份。选取K22和B73两个具有不同抗性水平的玉米自交系进行小斑病菌的室内接种。结果表明,48 h和72 h时,自交系K22叶片的病斑数、病斑面积和病斑面积比等各项指标均显著优于自交系B73,进一步验证田间鉴定的准确性。48 h或72 h时,高抗小斑病自交系K22与感病对照B73相比,5种防御酶活性增加14.32%～70.45%。     

基于分期播种试验夏玉米延迟收获促进子粒灌浆和粒重增益效应

基于2019-2022年夏玉米播期试验资料,分析夏玉米生理成熟后延迟收获对子粒农艺性状改善、增产效应。结果表明,早播10 d至晚播20 d处理的有效灌浆结束日均比生理成熟期延后,子粒有效灌浆持续日数依次比其花后10 d至生理成熟期日数延长28～9 d,播期每推迟10 d延长日数减少6.3 d。子粒农艺性状有明显的改善,百粒重、穗粒重提高,子粒增产效应明显,理论产量增产率16.95%～7.66%,增产量1 960.36～681.51 kg/hm²,对照增产最多,比晚播10、20 d增产粮食多2.4～2.9倍。为华北地区提高气候资源利用效率和粮食生产提质增效提供参考。     

臭氧浓度升高条件下大豆光合能力变化及光响应曲线的拟合模型比较

为研究臭氧(O3)浓度升高条件下大豆叶片光合能力、光响应曲线的变化以及光响应曲线拟合模型的适用性,以铁丰29为试验材料,于开顶式气室(OTCs)内进行盆栽试验,设置3个O3浓度(CK:45 nL·L-1,T1:80±10 nL·L-1,T2:120±10nL·L-1)熏蒸大豆,测量气体交换参数,使用非直角双曲线模型(N...     

ASP技术在"中国茶主题网关"建设中的应用

介绍了网站信息资源组织的新模式--主题网关的概念与特征;介绍了ASP技术的相关概念;并以中国茶主题网关建设为例,介绍了ASP技术在主题网关建设中的应用.