塑料薄膜覆盖晾晒亚麻技术初步研究与应用

本文研究分析了塑料薄膜覆盖晾晒亚麻技术的效应原理;较详细地介绍了主要技术措施;阐述了该技术在当地的应用情况.本项技术对亚麻生产具有一定的积极意义.     

Effectiveness and pathologic changes of transplantation of human mesenchymal stem cells in SOD1-G93A mouse model of familial amyotrophic lateral sclerosis

AIM: To study intravenous transplantation of human mesenchymal stem cells (hMSCs) on the life span and pathological change of SOD1-G93A amyotrophic lateral sclerosis (ALS) mice. METHODS: hMSCs were cultured and expanded from heparinized bone marrow cells from healthy donors and the purity and features were identified with FCM. hMSCs (3×106) resuspended in 0.3 mL DMEM or 0.3 mL DMEM only were injected into the tail vein of genotyped SOD1-G93A ALS mice. The mice were evaluated for signs of motor deficit with 4-point scoring system according to Weydt and the onset and life span were assessed. The pathological change was observed with Nissl staining and number of motor neuron was counted. RESULTS: The onset symptoms in untreated SOD1-G93A ALS mice appeared at (156.6±3.6) d of age and the average life span was (188.3±3.5) d. hMSCs transplantation delayed the onset of ALS type symptoms about 14 d and prolonged the life span about 18 d compared to the untreated SOD1-G93A littermates. The loss of motor neurons in untreated mice was much faster and severer than that in hMSCs transplanted mice. At 16 th week and 20 th week, motor neurons of untreated mice were significantly fewer than those of transplanted mice. β-globin gene in brain was detected in transplanted ALS mice. CONCLUSION: hMSCs migrate to central nervous system after intravenous transplantation, prolong the life span and delay the onset and motor neuron loss in SOD1-G93A ALS mice.     

气候变化下三裂叶豚草在新疆的潜在地理分布

明确有害入侵种在区域尺度上的潜在分布及其对气候变化的响应对入侵种的预警和具体防控意义重大。三裂叶豚草是全球公认的恶性入侵杂草,目前已经大面积入侵“一带一路”中亚枢纽—新疆伊犁河谷。为有效防控三裂叶豚草在新疆的扩散蔓延,本研究基于最大熵模型,预测了当前及未来两种气候情景（RCP4.5, RCP8.5）下2050s和2070s时期三裂叶豚草在新疆的潜在分布及变化趋势。结果表明：当前气候下三裂叶豚草在新疆的总适生面积达24.01万km²,约占全疆面积的14%,在RCP4.5情景下,至2050s、2070s时期将分别增至37.36万和39.23万km²;在RCP8.5情景下,将分别增至39.45万和42.94万km²。在未来两种气候情景下,随着时间的推移,潜在适生区总体均呈现向北增加转移的趋势,减少区域主要集中在准噶尔盆地。所有环境因子中,与降水相关的因子总贡献率为40.1%,与温度相关的因子总贡献率56.0%,其中最干月降水（36.2%）、温度季节性变化标准差（29.1%）是对三裂叶豚草分布贡献率较高的环境因子。耕地和建设用地是三裂叶豚草入侵风险最高的区域。建议管理的重点除了放在已预测到的适生区内,还应特别关注农田、草场、道路两侧这些人畜扰动大、水分充足的区域。     

松毛虫宽缘金小蜂生物学特性观察初报

本文对松毛虫宽缘金小蜂的形态特征、生活习惯及利用途径进行了详细的介绍，对防治松毛虫有一定的指导意义。     

Influence of predator stress on levels of IL-1β, IL-6 in BALB/C mice with brain asymmetry

AIM: To investigate the influence of predator stress on hypothalamic-pituitary-adrenal (HPA) axis and IL-1β, IL-6 in mice with brain asymmetry. METHODS: By using paw preference test, right-pawed, left-pawed, and ambidextrous-pawed mice model were established. Mice with brain lateralization were exposed to their predator (cat). After acute and chronic predator stress by cats, EIA and ELISA were applied to detect plasma levels of corticosterone (CS), IL-1β and IL-6. RESULTS: (1) The level of plasma CS: in both acute and chronic predator stress group, right-pawed and ambidextrous mice had a higher level than that in their corresponding normal group (P<0.05). Meanwhile, the two groups also showed higher level than left-pawed in acute stress. Compared with the corresponding group under acute stress, left-pawed had much higher level of CS under chronic stress (P<0.05). (2) The level of plasma IL-1β: in normal group, left and right-pawed mice were higher than that in ambidextrous mice (P<0.01), of which left-pawed was the highest. In chronic stress group, the left-pawed exhibited higher level than that in right-pawed mice (P<0.05). (3) The level of IL-6: after chronic predator stress, ambidextrous mice had much higher level than that in other two groups (P<0.05). CONCLUSION: Under predator stress, the HPA axis and plasma proinflammatory cytokines (IL-1β, IL-6) in BALB/C mice were modulated by the brain asymmetrically, and brain lateralization may influence the function of immuno-endocrine network in psychological circumstances.     

两个园林树种防寒技术的研究

以日本厚朴和山樱引种实生苗为试材,综合引种过程中日本厚朴和山樱的越冬表现,分析不同越冬防护措施与植株冻伤率、生长状况之间的关系,探讨沈阳地区引种日本厚朴和山樱栽培的最佳方式和越冬防寒措施,制定适宜的栽培管理措施,解决日本厚朴在北方地区自然条件下安全越冬问题.     

二苯胺处理对鸭梨果实冷害的影响

研究了贮前二苯胺（DPA）处理对在低温条件下（0℃）贮藏鸭梨果实的果心褐变及生理变化的影响。结果表明：急骤降温（0℃）可产生严重的早期果心褐变,贮前二苯胺处理在一定条件下可减轻鸭梨果实的早期褐变,贮藏60 d时DPA处理果实的褐变指数为0.233,而对照为0.417;同时DPA处理可降低果实的乙烯释放量、抑制多酚氧化酶活性、保持果实酚类物质含量,对丙二醛（MDA）含量和果实相对电导率也有一定影响。     

纳米标记免疫层析法在农药残留检测中的应用研究进展

农药残留超标已成为影响农产品质量安全的重要问题,迫切需要探寻开发灵敏、准确、可靠、便捷且适用性强的农药残留快速检测方法。免疫层析法是将抗原抗体特异性免疫反应和色谱层析分离技术相结合的一种快速检测方法,其中,基于胶体金标记的免疫层析技术以其便捷、成本低、可视化等优点而受到普遍欢迎。近年来随着量子点、时间分辨荧光微球、上转换发光纳米粒子等新型纳米标记材料的出现,免疫层析技术得到了广泛发展。文章从标记类型（非共价作用标记及共价作用标记）及标记材料（胶体金、纳米碳、量子点、上转换发光纳米粒子、磁性纳米颗粒、时间分辨荧光微球及荧光乳胶颗粒）等方面,综述了不同纳米材料标记的免疫层析技术及其在农药残留检测领域的研究及应用进展,可为深入开展农药残留免疫层析技术研究提供参考。     

几种生长调节剂对四翅滨藜全光喷雾扦插效果的影响

四翅滨藜全光照喷雾嫩枝扦插试验结果表明:采用NAA、ABT 2号生根粉、IBA等3种外源植物生长调节剂处理插条2 h,对插条具有良好的促根作用,其中100 mg/L NAA为最好,生根率可达到93.6%;100 mg/LABT2号生根粉和100 mg/L IBA次之,生根率分别可以达到92.6%,92.3%.     

鸡白细胞介素-2分子生物学和疾病防治应用研究进展

鸡白细胞介素-2(IL-2)是由激活的T细胞分泌产生的糖蛋白,具有显著的免疫增强作用,不仅在T细胞、B细胞的生长与分化和NK细胞的激活等方面发挥重要作用,而且与其他细胞因子一起发挥免疫功能,是一种能影响机体免疫反应各个方面的调节因子。文章综述了鸡白细胞介素-2分子生物学和疾病防治应用研究进展。