Antiproliferation effects of 188Re labeled monoclonal antibody on prostate cancer cells in vitro

AIM: To investigate the effect of 188Re labeled monoclonal antibody on prostatic specific membrane antigen 7E11C5.3,radioimmunotherapy for the treatment of human prostate cancer cell line LNCaP in vitro.METHODS: 188Re-7E11C5.3 was prepared by direct 2-mercaptoethanol reduction method.Labeling efficiency and radiochemical purity was measured by paper chromatography.Immunoreactive fraction was determined by linear extrapolation.Cytotoxicity to LNCaP cells was determined by MTT assay.RESULTS: The labeling yield of 188Re-7E11C5.3 was (93.16±2.18)%,the radiochemical purity was (95.62±0.48)%,and the immunoreactive fraction was (74.86±1.86)%.The inhibitory effect of 188Re-7E11C5.3 on cell proliferation of LNCaP cells was significantly higher than that of 188Re-mIgG or 188ReO-4.The 50% inhibitory doses (IC50) of 188Re-7E11C5.3,188Re-mIgG,and 188ReO-4 were (23.38±3.73)×107 Bq/L,(59.21±8.02)×107 Bq/L and (68.89±10.91)×107 Bq/L,respectively.CONCLUSION: 188Re-7E11C5.3 can effectively inhibit the growth of in vitro cultured prostate cancer cells and shows much potential for prostate cancer radioimmunotherapy.     

Significance of expression of extracellular matrix metalloproteinase inducer and endothelin converting enzymes in lymphoid metastasis and prognosis in non-small-cell lung carcinoma

AIM: To investigate the expression of extracellular matrix metalloproteinase inducer (EMMPRIN) and endothelin converting enzymes (ECE) in non-small-cell lung carcinoma (NSCLC), and their relationship with lymphoid metastasis and prognosis.METHODS: Expression of EMMPRIN and ECE in 77 cases of patients with NSCLC was detected immunohistochemically.The relationship of the expression of EMMPRIN and ECE with tumor size, histological type, differentiation, lymphoid metastasis, clinical stage, and prognosis were analyzed.RESULTS: The expressive rate of EMMPRIN and ECE was 66% and 45%, respectively.The expression of EMMPRIN and ECE was associated positively with lymphoid metastasis (both P<0.01, r=0.371 and 0.467, respectively), and inversely with survival time (P<0.01 and P<0.01).No relationship was found in the expression of EMMPRIN and ECE with tumor size, histological type, and differentiation (P>0.05).The expression of EMMPRIN was associated with the expression of HGF in NSCLC (P<0.05, r=0.243).CONCLUSION: The expression of EMMPRIN and ECE is associated with lymphoid metastasis and prognosis in NSCLC.Overexpression of EMMPRIN and ECE implies infavourable prognosis in NSCLC.     

对当前桂林市柑桔黄龙病综合治理工作的意见

桂林具得天独厚的气候及地理条件,是柑桔种植的最适宜区,种柑桔已有几十年的历史,20世纪80年代规模及产量达到了前所未有的辉煌,90年代中后期由于受黄龙病的严重危害,产量和品质急剧下降,大量桔园被毁、丢弃,柑桔产业一度跌入低迷状态.进入新世纪,各级政府把重振桂林柑桔产业提上议事日程,进行大规模农业产业结构调整,掀起了种柑桔高潮,2005年全市柑桔栽培面积达8.3万hm2,总产量105万t,总产值16.07亿元,分别占当年全市水果面积、总产量、总产值的42.95%、63.25%和52.23%.按总产值计算,全市当年农民人均柑桔生产收入412元,占当年农民人均收入的56.2%,不论是现在还是在今后相当长的一段时间内,都还没有哪个水果品种可以代替柑桔在桂林市农业和农村经济中的主导地位和作用.因而,柑桔黄龙病综合治理(以下简称"治黄")也被提上议事日程,尤其是2005年12月12日"恭城会议"后,桂北大地"治黄"工作如火如荼,但"治黄"不是一蹴而就的,它将是一个复杂的、艰巨的、漫长的持续过程,"治黄"还必须把握好如下几个问题.     

鸡的铜绿假单胞菌病的研究

１９９８年６－１１月天津武清,蓟县,河北玉田等地出现一种以眼睑肿胀,流泪,瞎眼及喙端,眼,践爪结痂为特征的鸡传染病。病鸡主要为３０－６０日龄。经鼻感染３０日龄鸡复制出了与自然病例相同的临床表现,并分离到接种菌。对分离菌的系统鉴定表明所分离的４株菌均为铜绿假单胞菌。