蓟马采集和玻片标本的制作

\t\t\t\t\t目的\t\t\t\t\t丰富中国呆蓟马属团蓟马种类,为呆蓟马属团的鉴定提供参考信息。\t\t\t\t\t\t\t\t\t\t\t\t\t方法\t\t\t\t\t标本采集采用拍盘法,标本保存在75%的酒精中,带回室内进行玻片标本制作,在生物显微镜下进行鉴定。\t\t\t\t\t\t\t\t\t\t\t\t\t结果\t\t\t\t\t记述了蓟马亚科呆蓟马属团一中国新纪录属塔姆蓟马属(Tameothrips)及1新纪录种芦竹塔姆蓟马(T. arundo),详细描述了该种的形态鉴定特征,编制了中国已知呆蓟马属团属种名录及分属检索表。玻片标本保存于云南农业大学植物保护学院昆虫系。\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t结论\t\t\t\t\t塔姆蓟马属(Tameothrips)为中国新纪录属,中国目前已记录呆蓟马属团9属21种。\t\t\t\t\t\t\t\t\t     

胡麻异质型ACCase亚基基因的克隆与表达分析

为研究胡麻异质型乙酰辅酶A羧化酶BCCP、BC、α-CT和β-CT四个亚基基因的生物学功能,采用基因克隆、生物信息学和RT-PCR技术分别对胡麻accA、accB、accC和accD 4个基因进行分析。结果发现陇亚10号accA基因编码α-CT亚基,CDS序列全长为2364 bp,编码787个氨基酸;accB基因编码BCCP亚基,CDS序列全长为1173 bp,编码275个氨基酸;accC基因编码BC亚基,CDS序列全长为1574 bp,编码527个氨基酸;accD基因编码β-CT亚基,CDS序列全长为1137 bp,编码378个氨基酸,且4个基因编码的蛋白都是脂肪酸系数较高的亲水性蛋白。RT-PCR结果表明,胡麻accA、accB、accC和accD 4个基因在3个胡麻品种(高含油量的张亚2号、中含油量的陇亚10号和低含油量的R2-17)的不同时期不同组织的表达模式不同,accA、accB、accC和accD四个基因在R2-17、陇亚10号和张亚2号3个胡麻品种所有时期的不同组织中均有表达,但在种子中的表达量均明显高于其他组织,尤其在种子发育10~25 d油脂快速积累时期,4个基因的表达量都迅速增加并达到最高峰。研究表明异质型乙酰辅酶A羧化酶基因可能调控胡麻种子发育前期油脂的合成积累。     

Association of tumor budding with tumor-infiltrating lymphocytes and prognosis in breast cancer patients

AIM: To investigate the relationship of tumor budding with clinicopathologic parameters, tumor-infiltrating lymphocytes (TILs) of tumor microenvironment and the prognosis in breast cancer patients.METHODS: A total of 178 HE section samples were collected from the breast cancer patients treated with surgery in the First Affilated Hospital of Jinan University during Jan. 2012 to Dec. 2016. The tumor budding and stromal tumor-infiltrating lymphocytes were observed under light microscope. The correlation of tumor budding with the clinicopathologic status and TILs were analyzed by χ² test. Kaplan-Meier survival analysis and Log-rank test were used to estimate the disease-free survival (DFS) and overall survival (OS).RESULTS: High tumor budding level was associated with more positive lymph nodes, higher grade, and more lymphovascular invasion. In addition, the patients with higher tumor budding level showed fewer TILs, while the patients with lower tumor budding level had more TILs. Furthermore, the patients with higher tumor budding level had a worse disease-free survival and overall survival than those with lower tumor budding level.CONCLUSION: Tumor budding is significantly associated with adverse clinicopathological characteristics of breast cancer and negatively correlated with TILs. Therefore, tumor budding may serve as a potential biomarker to predict the prognosis of breast cancer.     

Effects of puerarin on apelin-12, angiotensin II,NO and blood pressure in renovascular hypertensive rat

AIM: To investigate the effects of puerarin on blood pressure in renovascular hypertensive rats, and to measure puerarin-induced changes of apelin-12, angiotensin II (Ang II) and nitric oxide(NO), the factors related to development of hypertension. METHODS: Sixty-five male Sprague-Dawley rats were used, of which 8 rats were randomly selected as sham operation group, and the remaining were used to make two-kidney, one-clip model. The rats that met the criterion for Goldblatt hypertensive rat model were randomly allocated into 5 groups: high-, middle- and low-dose puerarin groups, captopril group, and model group. The drugs were administered for 6 weeks. Blood pressure was measured every 2 weeks. Six weeks after treatment, all rats were sacrificed under deep anesthesia. Blood and kidney samples were collected. The level of apelin-12 in serum and kidneys was detected by ELISA. The level of Ang II in plasma and kidneys was measured by radioimmunoassay. NO level in serum was examined by nitrate reductase assay. RESULTS: Puerarin had an antihypertensive effect in a dose-dependent manner. Marked decreases in the level of serum apelin-12 in high- and middle-dose puerarin groups were observed(P<0.01). Puerarin at low dose did not cause obvious change in the content of apelin but still reached significant level (P<0.05). As the dose of puerarin went up, the level of apelin-12 in the kidneys was gradually decreased. Puerarin at high and middle doses obviously reduced the level of AngII in plasma, while purarin at low dose did not produce any significant effects. Puerarin at high and middle doses markedly increased the level of NO in serum, but puerarin at low dose did not induce any significant changes. CONCLUSION: Puerarin has an antihypertensive effect, and its mechanism may be related to inducing the changes of apelin, Ang II and NO, and regulating the balance among those factors.     

响应面法优化Xenorhabdus nematophila发酵培养基的研究

【目的】提高Xenorhabdus nematophilaYL001的抗菌活性,为该菌株杀菌活性成分的提取分离及生物农药的开发应用奠定基础。【方法】以YSG培养基为基础,采用单因子试验方法,对最佳碳源和氮源进行筛选,并采用全因子中心组合试验设计和响应面法对其最佳配比进行优化。【结果】YL001菌株的最佳碳源为玉米粉,氮源为豆饼粉,培养基的最佳组成为:玉米粉9.69 g/L,豆饼粉76.50 g/L,MgSO41.07 g/L,(NH4)2SO41.79 g/L,KH2PO40.63 g/L,K2HPO40.80 g/L,Na2SO41.25 g/L,在此条件下,YL001菌株抗菌活性达268.9 U/mL。【结论】培养基优化后YL001菌株的抗菌活性与试验的预测值接近,表明响应面法在培养基优化中十分有效,相对简单,且节省时间和材料。     

Statistical Model on the Time and Time Difference Error of Arrival in TDOA Location Estimation

Additional time delay of arrival resulted from non-line of sight(NLOS)propagation and muti-path effect in(cellular) environment is the dominant factor resulting in TDOA location error,study on the statistical property of TDOA error is useful for improving the accuracy of TDOA location.Based on the exponential distribution property of NLOS propagation delay,and considering the system error resulted from the signal detection,the authors establish a statistical model of arrival time and a model of TDOA error distribution by statistics when non-light of sight and multi-path propagation exist.The models reflect the statistical property of arrival time and TDOA error in cell network,the simulation shows that the proposed models have a good accuracy and a satisfactory behavior.     

不同密度对棉花根系生长与分布的影响

 利用根钻挖掘法研究了大田条件下6个种植密度对棉花根系生长分布的影响。结果表明：1.5万～8.7万株·hm^-2密度水平根干物质质量密度、根长密度在吐絮之前持续增加,而10.5万株·hm^-2处理在盛铃期后出现下降。在盛蕾期至初花期,棉花根干物质质量密度、根长密度均随种植密度的增加而增大;在盛花期至吐絮初期,根干物质质量密度以3.3万株·hm-2处理最高,3.3万～10.5万株·hm^-2处理根长密度显著高于1.5万株·hm^-2。根系垂直分布结果表明,0～30 cm土层内的根干物质质量和根长分别占所测土体中根量的67.80%～97.44%和54.01%～93.33%。低密度处理（1.5万株·hm^-2、3.3万株·hm^-2）的浅层（0～20 cm）根干物质质量密度和根长密度在盛蕾期均低于其它处理,深层（30～60 cm）根干物质质量密度和根长密度在初花期至盛铃期低于其它处理。吐絮初期,各密度处理根长密度在深层土壤有显著差异。     

新疆降水研究进展综述

目的研究转基因棉花在荒地及栽培地的生存竞争能力及环境适应性,为其环境安全性评价研究提供理论依据。方法以转KatG和SacB基因的抗旱棉花为研究对象,在荒地以及栽培地中研究转基因抗旱棉花的环境适应、生存竞争能力以及对农田生物群落的影响,结合新疆气候条件,分析转基因棉花在荒地条件下演变成杂草的可能性,利用生态学信息论方法分析种植转基因抗旱棉花对生物群落的影响。结果3个试验区域内越冬试验材料均未能出苗,荒地生存竞争试验材料出苗率较低,出苗率最高为47.3%;植株矮小,株高最高为39.8 cm;生育期内未能开花结铃。3个试验区域杂草群落的香农—维纳指数最大为2.232,最小为2.070,均匀度指数最大为0.920,最小为0.737,相同试验区域的不同棉花种植区域间均无显著性差异(P>0.05);不同播种深度下杂草群落的香农—维纳指数最大为1.603,最小为1.188,均匀度指数最大为0.874,最小为0.615,且相同棉花材料间杂草的香农—维纳指数无显著性差异(P>0.05);不同的种植密度下杂草群落的香农—维纳指数最大为1.455,最小为1.263,均匀度指数最大为0.918,最小为0.615,且相同棉花材料间杂草的香农—维纳指数无显著性差异(P>0.05)。结论转基因抗旱棉花在新疆地区荒地的环境适应性较弱,落粒棉种没有越冬的可能,演变成杂草的可能微乎其微;在荒地生态区域中与其他物种间生存竞争处于劣势,不会显著影响种植区域内的群落多样性及物种分布。     

发菜生存与生长分布的微生态环境

通过野外调查 ,研究和分析了对发菜生存与生长发育起直接作用的微土壤、微气候、微地形、微植被和风向等微生态环境。发菜对生存环境的选择性很强。其中 ,干一湿交替的微气候环境是一个起决定作用的关键性因子 ,是发菜生存和生长发育的必要条件。一方面 ,发菜藻体在干燥的状态下 ,有利于干物质的积累 ,是发菜生存和生长的必要前提 ;另一方面 ,发菜藻体只有在一定湿润状态下 ,才能生长发育。由于干旱气候环境所限 ,野生发菜的生长速度极为缓慢。     

酶联免疫吸附方法检测禽流感病毒的研究进展

禽流感是由禽流感病毒(AIV)引起的人畜共患病,可以引起巨大的经济损失,严重威胁人类健康。酶联免疫吸附试验(ELISA)具有灵敏度高、可标准化操作、结果易于分析等特点,因此在禽流感病毒检测过程中占据非常重要的地位。本文重点介绍几种主要的禽流感病毒酶联免疫吸附试验方法,并就该方法的发展提出建议。