不同培养料对姬菇产量和品质的影响

比较分析了闽牧42、蔗渣和稻草为主料栽培的姬菇产量、生物学效率和蛋白氨基酸含量。结果表明，闽牧42培养料栽培的姬菇，生物学效率最高，为1.14。变量分析表明，闽牧42培养料栽培的姬菇比稻草培养料栽培的姬菇增产60.11%，差异达极显著水平；比蔗渣培养料栽培的姬菇增产19.28%，差异达显著水平。蔗渣培养料栽培的姬菇比稻草培养料栽培的姬菇增产34.23%，差异也达到显著水平。三种培养料栽培的陈旧菇，粗蛋白含量均高于31%，氨基酸总量三者相近，均高于22%，E/T 值达37%左右，接近FAO/WHO提出的40%的参考模式。     

Mechanism of arrhythmias caused by autoantibodies against β1-adrenoceptors induced by hepatitis B virus in mice

AIM: To investigate the significance of autoantibodies against β1-adrenoceptors induced by hepatitis B virus in the pathogenesis of hepatitis virus myocarditis. METHODS: 30 mice were injected peritoneally with an emulsion of hepatitis B virus and complete Freund's adjuvant every three weeks. The autoantibodies were examined by ELISA, the heart and liver specimens were collected on 56 d for pathological observation and the binding of the autoantibodies to guinea pig cardiac myocytes were examined by immunofluorescence. Using the patch clamp technique, the effects of 1∶50 autoantibodies purified by octanoic acid extraction on the action potential and L type Ca2+ currents of guinea pig cardiac myocytes were also investigated. RESULTS: There was a good correlation between the autoantibodies and hepatitis B virus. Without pathological changes in the heart and liver specimens, 6 mice of the test group manifested bundle branch block, sinus arrest and premature ventricular beat etc, which were positive in the autoantibodies. The specific binding of the autoantibodies of the mice to guinea pig cardiac myocytes was observed. 1∶50 autoantibodies of the mice prolonged APD20, APD50, APD90 by 36.46%, 29.63% and 12.40%, respectively and enhanced L type Ca2+ currents by (49.67±16.01)%. CONCLUSIONS: Autoantibodies against β1-adrenoceptors of the mice induced by hepatitis B virus result in several arrhythmias, which might be mediated by the enhancement of L type Ca2+ currents.     

江西省不同地方中华蜜蜂外部形态的比较研究

主要测定了江西省 8个县 (市 )中蜂工蜂的吻长、右前翅长与宽、背板长、肘脉指数和跗节指数等主要外部形态指标 ,结果表明 ,浮梁中蜂为最理想选育材料。同时 ,对江西省中蜂资源的开发利用提出几点建议。     

沥青混凝土路面裂缝防治措施的探讨

本文通过分析裂缝产生的原因，提出防止路面产生裂缝的几点措施。     

耐氟喹诺酮类药物大肠埃希氏菌基因突变的MAMA-PCR检测

采用MAMA-PCR法对72株已知突变菌株的突变情况进行了检测。结果，有2种gyrA83位突变：TCG→TTG和TCG缺失；4种gyrA87位突变：GAC→AAC、GAC→GGC、GAC→TAC和GAC→CAC；1种parC80位突变：AGC→ATC以及4种parC84位突变：GAA→AAA、GAA→GCA、GAA→GGA和GAA→GTA。采用同样的方法，对38株未知突变菌株的检测结果显示，gyrA83、gyrA87、parC 80和parC84位的突变检出的正确率分别为97．4％、94．7％、81．6％和94．7％。结果表明，MAMA-PCR有望成为监测耐氟喹诺酮类药物大肠埃希氏菌的分子生物学常规方法。     

深化闽台农业合作的战略思考

本文通过分析闽台农业合作发展现状与存在的问题,对其战略地位进行再认识,从而提出闽台农业合作战略转型与策略谋划的新思考,以期为持续发挥福建在海峡两岸农业合作中的引领作用,实现闽台农业合作更高层次的双赢,进一步提升福建现代农业综合竞争力,为海峡西岸社会主义新农村建设提供决策参考。     

练苗期营养液浓度对黄瓜幼苗贮运质量的影响

以黄瓜幼苗为试材,采用练苗期喷施不同营养液浓度的方法,研究练苗期不同营养液浓度处理(T1、T2、T3、T4)对不同贮存天数(0、2、4d)下黄瓜幼苗主要形态指标的变化规律与叶绿素含量的变化情况,以及定植后植株恢复能力的影响。结果表明:在短期(0、2d)贮运黄瓜幼苗时,将练苗期营养液浓度控制在1/2倍液有利于较好的保持幼苗长势,缩短缓苗期有利于幼苗定植后恢复生长;在中期(4d)贮运黄瓜幼苗时,练苗期营养液浓度适宜选择1/4倍液、1/2倍液,能够保持较好的商品苗品质,利于幼苗定植后存活,缩短缓苗期。     

铁皮石斛的特征特性及大棚栽培新技术

本文通过介绍铁皮石斛的特征特性及从大棚建设技术、移栽、栽培料处理、种植管理、病虫害管理等方面总结其大棚栽培技术要点,为生产上进一步推广应用提供参考.     

Preparation of recombinant PTD-HSP27 and verification of its ability to penetrate the cell membrane of human lens epithelial cells and rabbit cornea

AIM: To construct the prokaryotic expression system containing protein transduction domain (PTD) with heat shock protein 27 (HSP27) in order to prepare and purify the recombinant protein, and to verify whether the recombinant protein PTD-HSP27 has the ability to penetrate the human lens epithelial cell (HLEC) membrane and the rabbit cornea. METHODS: The plasmid pKYB-PTD-HSPB1-6His was constructed by the technique of overlap extension PCR. The plasmid was transformed and PTD-HSP27 was purified through nickel affinity chromatography column and identified by Western blotting. PTD-HSP27-6His was labeled with the fluorescein isothiocyanate (FITC). The penetrating ability of PTD-HSP27 into HLECs and rabbit cornea was tested. RESULTS: The recombinant PTD-HSP27 plasmid was successfully cloned and effectively expressed. The correctness of the recombinant protein PTD-HSP27 was demonstrated. Fluorescence microscopic examination showed that PTD-HSP27-FITC was internalized by HLECs. Fluorescent labeled PTD-HSP27 was then observed in the rabbit aqueous humor. CONCLUSION: The recombined gene PTD-HSPB1 was constructed by overlap extension PCR technique and the PTD-HSP27 fusion protein was prepared and purified by nickel affinity chromatography column. Using the technique of PTD-fusion protein, HSP27 was transduced into HLECs and passed through the cornea.     

Preventive effects of Clostridium butyricum on gastric ulceration induced by pylorus ligature in mice

AIM: To investigate the preventive effects of Clostridium butyricum (C. butyricum) on the type of pylorus ligated gastric ulcer (GU) in mice and the underlying mechanisms. METHODS: ICR mice were randomly divided into 4 groups: sham operation group, model group, C. butyricum pretreatment group and omeprazole pretreatment group. Gastric pyloric ligation was adopted to establish GU model in mice. The gastric juice was collected to measure the content of gastric free mucus, the pH of gastric juice and the activity of pepsin. The gastric tissues were collected for routine HE staining to observe the pathological changes. The content of glycogen was detected by PAS staining. The protein expression of Bax and Bcl-2 in the gastric mucosa was also assessed by immunohistochemical staining. RESULTS: The HE and PAS staining showed that the C. butyricum pretreatment obviously attenuated the mucosa lesion induced by ligation. Compared with model group, the pH of gastric juice was significantly raised. The activity of pepsin fell off in C. butyricum group, which was lower than that in omeprazole group. In comparison with model group, the content of gastric free mucus was dramatically increased and PAS staining showed a significant rise in C. butyricum group, but not in omeprazole group. The protein expression of Bax was decreased and the protein expression of Bcl-2 was upgraded in C. butyricum group than those in model group. CONCLUSION: C. butyricum protects gastric mucosa against the challenge of pylorus ligation in mice and its mechanism may be related to inhibiting gastric acid secretion and the activation of pepsin, increasing the production of gastric free mucus, strengthening the expression of bcl-2 gene and inhibiting the expression of bax gene.