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排序方式: 共有1092条查询结果,搜索用时 171 毫秒
81.
Rensing SA Lang D Zimmer AD Terry A Salamov A Shapiro H Nishiyama T Perroud PF Lindquist EA Kamisugi Y Tanahashi T Sakakibara K Fujita T Oishi K Shin-I T Kuroki Y Toyoda A Suzuki Y Hashimoto S Yamaguchi K Sugano S Kohara Y Fujiyama A Anterola A Aoki S Ashton N Barbazuk WB Barker E Bennetzen JL Blankenship R Cho SH Dutcher SK Estelle M Fawcett JA Gundlach H Hanada K Heyl A Hicks KA Hughes J Lohr M Mayer K Melkozernov A Murata T Nelson DR Pils B Prigge M Reiss B Renner T Rombauts S Rushton PJ 《Science (New York, N.Y.)》2008,319(5859):64-69
We report the draft genome sequence of the model moss Physcomitrella patens and compare its features with those of flowering plants, from which it is separated by more than 400 million years, and unicellular aquatic algae. This comparison reveals genomic changes concomitant with the evolutionary movement to land, including a general increase in gene family complexity; loss of genes associated with aquatic environments (e.g., flagellar arms); acquisition of genes for tolerating terrestrial stresses (e.g., variation in temperature and water availability); and the development of the auxin and abscisic acid signaling pathways for coordinating multicellular growth and dehydration response. The Physcomitrella genome provides a resource for phylogenetic inferences about gene function and for experimental analysis of plant processes through this plant's unique facility for reverse genetics. 相似文献
82.
In Caenorhabditis elegans, the gonad acquires two U-shaped arms by the directed migration of its distal tip cells (DTCs) along the body wall basement membranes. Correct migration of DTCs requires the mig-17 gene, which encodes a member of the metalloprotease-disintegrin protein family. The MIG-17 protein is secreted from muscle cells of the body wall and localizes in the basement membranes of gonad. This localization is dependent on the disintegrin-like domain of MIG-17 and its catalytic activity. These results suggest that the MIG-17 metalloprotease directs migration of DTCs by remodeling the basement membrane. 相似文献
83.
A 49-kilodalton phosphoprotein in the Drosophila photoreceptor is an arrestin homolog 总被引:10,自引:0,他引:10
T Yamada Y Takeuchi N Komori H Kobayashi Y Sakai Y Hotta H Matsumoto 《Science (New York, N.Y.)》1990,248(4954):483-486
The gene encoding the 49-kilodalton protein that undergoes light-induced phosphorylation in the Drosophila photoreceptor has been isolated and characterized. The encoded protein has 401 amino acid residues and a molecular mass of 44,972 daltons, and it shares approximately 42 percent amino acid sequence identity with arrestin (S-antigen), which has been proposed to quench the light-induced cascade of guanosine 3',5'-monophosphate hydrolysis in vertebrate photoreceptors. Unlike the 49-kilodalton protein, however, arrestin, which appears to bind to phosphorylated rhodopsin, has not itself been reported to undergo phosphorylation. In vitro, Ca2+ was the only agent found that would stimulate the phosphorylation of the 49-kilodalton protein. The phosphorylation of this arrestin-like protein in vivo may therefore be triggered by a Ca2+ signal that is likely to be regulated by light-activated phosphoinositide-specific phospholipase C. 相似文献
84.
Cartilage acidic protein-1B (LOTUS), an endogenous Nogo receptor antagonist for axon tract formation
Sato Y Iketani M Kurihara Y Yamaguchi M Yamashita N Nakamura F Arie Y Kawasaki T Hirata T Abe T Kiyonari H Strittmatter SM Goshima Y Takei K 《Science (New York, N.Y.)》2011,333(6043):769-773
Neural circuitry formation depends on the molecular control of axonal projection during development. By screening with fluorophore-assisted light inactivation in the developing mouse brain, we identified cartilage acidic protein-1B as a key molecule for lateral olfactory tract (LOT) formation and named it LOT usher substance (LOTUS). We further identified Nogo receptor-1 (NgR1) as a LOTUS-binding protein. NgR1 is a receptor of myelin-derived axon growth inhibitors, such as Nogo, which prevent neural regeneration in the adult. LOTUS suppressed Nogo-NgR1 binding and Nogo-induced growth cone collapse. A defasciculated LOT was present in lotus-deficient mice but not in mice lacking both lotus- and ngr1. These findings suggest that endogenous antagonism of NgR1 by LOTUS is crucial for normal LOT formation. 相似文献
85.
Periodic signaling controlled by an oscillatory circuit that includes protein kinases ERK2 and PKA 总被引:1,自引:0,他引:1
Maeda M Lu S Shaulsky G Miyazaki Y Kuwayama H Tanaka Y Kuspa A Loomis WF 《Science (New York, N.Y.)》2004,304(5672):875-878
Self-regulating systems often use robust oscillatory circuits. One such system controls the chemotactic signaling mechanism of Dictyostelium, where pulses of adenosine 3',5'-monophosphate (cAMP) are generated with a periodicity of 7 minutes. We have observed spontaneous oscillations in activation of the mitogen-activated protein (MAP) kinase ERK2 that occur in phase with peaks of cAMP, and we show that ERK2 modulates cAMP levels through the phosphodiesterase RegA. Computer modeling and simulations of the underlying circuit faithfully account for the ability of the cells to spontaneously generate periodic pulses during specific stages of development. Similar oscillatory processes may occur in cells of many different species. 相似文献
86.
色泽是金针菇的重要性状之一,受一对等位基因的控制。以FL19(黄色)、8801(白色)及其后代(F1代、F2代)菌株和相应单核菌株为材料,采用分离群体分组分析(BulkedSegregantAnalysis,BSA)方法,对金针菇基因组DNA进行RAPD分析,通过对200个引物的筛选,获得了一个与白色性状基因紧密连锁的RAPD标记S3751800,并在亲本及其后代菌株进行了验证,具有较好的重复性和稳定性。可用于白色金针菇新品种的辅助选育。 相似文献
87.
Terracciano A Abdel-Khalek AM Adám N Adamovová L Ahn CK Ahn HN Alansari BM Alcalay L Allik J Angleitner A Avia MD Ayearst LE Barbaranelli C Beer A Borg-Cunen MA Bratko D Brunner-Sciarra M Budzinski L Camart N Dahourou D De Fruyt F de Lima MP del Pilar GE Diener E Falzon R Fernando K Ficková E Fischer R Flores-Mendoza C Ghayur MA Gülgöz S Hagberg B Halberstadt J Halim MS Hrebícková M Humrichouse J Jensen HH Jocic DD Jónsson FH Khoury B Klinkosz W Knezević G Lauri MA Leibovich N Martin TA 《Science (New York, N.Y.)》2005,310(5745):96-100
Most people hold beliefs about personality characteristics typical of members of their own and others' cultures. These perceptions of national character may be generalizations from personal experience, stereotypes with a "kernel of truth," or inaccurate stereotypes. We obtained national character ratings of 3989 people from 49 cultures and compared them with the average personality scores of culture members assessed by observer ratings and self-reports. National character ratings were reliable but did not converge with assessed traits. Perceptions of national character thus appear to be unfounded stereotypes that may serve the function of maintaining a national identity. 相似文献
88.
Excess cyclin E-Cdk2 accelerates entry into S phase of the cell cycle and promotes polyploidy, which may contribute to genomic instability in cancer cells. We identified 20 amino acids in cyclin E as a centrosomal localization signal (CLS) essential for both centrosomal targeting and promoting DNA synthesis. Expressed wild-type, but not mutant, CLS peptides localized on the centrosome, prevented endogenous cyclin E and cyclin A from localizing to the centrosome, and inhibited DNA synthesis. Ectopic cyclin E localized to the centrosome and accelerated S phase entry even with mutations that abolish Cdk2 binding, but not with a mutation in the CLS. These results suggest that cyclin E has a modular centrosomal-targeting domain essential for promoting S phase entry in a Cdk2-independent manner. 相似文献
89.
The molecular nature of sweet taste receptors has not been fully explored. Employing a differential screening strategy, we identified a taste receptor gene, Tre1, that controls the taste sensitivity to trehalose in Drosophila melanogaster. The Tre1 gene encodes a novel protein with similarity to G protein-coupled seven-transmembrane receptors. Disruption of the Tre1 gene lowered the taste sensitivity to trehalose, whereas sensitivities to other sugars were unaltered. Overexpression of the Tre1 gene restored the taste sensitivity to trehalose in the Tre1 deletion mutant. The Tre1 gene is expressed in taste sensory cells. These results provide direct evidence that Tre1 encodes a putative taste receptor for trehalose in Drosophila. 相似文献
90.
Episodic memory or memory for the detailed events in our lives is critically dependent on structures of the medial temporal lobe (MTL). A fundamental component of episodic memory is memory for the temporal order of items within an episode. To understand the contribution of individual MTL structures to temporal-order memory, we recorded single-unit activity and local field potential from three MTL areas (hippocampus and entorhinal and perirhinal cortex) and visual area TE as monkeys performed a temporal-order memory task. Hippocampus provided incremental timing signals from one item presentation to the next, whereas perirhinal cortex signaled the conjunction of items and their relative temporal order. Thus, perirhinal cortex appeared to integrate timing information from hippocampus with item information from visual sensory area TE. 相似文献