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31.
地处阴山山系中段大青山脉林区的哈园,其气候区划为我国中温带亚干旱气候类型。就整体而言,四季分明,内陆气候特征强烈;但因巨大阴山山地效应,年降雨量超过450mm,植被繁茂,形成多种多样的小气候环境,以高山环境、森林环境及峡谷环境为代表,它们冷暖干湿分异形大。气候的多样性,可满足旅游者的不同要求,产生了非凡吸引力,为哈园开发建设的宝贵自然资源之一。 相似文献
32.
纹枯病菌絲在田間稻株上的消长受水稻各生育期、及当时气候条件影响很大;一般在孕穗职前菌絲量少,孕穗至乳熟初期分布量大、蔓延速度快;抽穗期后10天达到最高峯。早稻成熟期菌絲停止蔓延或消失;晚稻(尤其連作晚稻)抽穗期后因寒潮或低温而停止蔓延;乳熟期趋于消失。纹枯病菌核的发育一般要通过四个时期,无休眠期。病情的水平扩展速度以孕穗至抽穗期最快,乳熟期几停止;而垂直扩展速度以水稻分蘖期最慢,抽穗至乳熟期最快。矮稈品种2—3天,高稈品种3—5天便可士升一个叶位,乳熟后緩慢。晚稻則以抽穗期最快,抽穗后緩慢,乳熟后病势停滞不前。在水稻易感期(孕穗—抽穗),遇到梅雨的两端低温,职及矮稈品种、多肥、密植,在一定有效菌核量下,是本病流行期、流行程度的关鍵因素。防治适期应以菌絲蔓延前期,卽在分蘖期至孕穗期施药,以杀伤菌絲为主;有必要时可在抽穗期再施药一次,保护剑叶,以抑制菌核形成、兼杀菌絲为主。如对矮稈品种、多肥栽培的水稻,于分蘖期施药保护是很必要的。 相似文献
33.
A. Schots J. De Boer A. Schouten J. Roosien J. F. Zil Verentant H. Pomp L. Bouwman-Smits H. Overmars F. J. Gommers B. Visser W. J. Stiekema J. Bakker 《European journal of plant pathology / European Foundation for Plant Pathology》1992,98(2):183-191
Engineering resistance against various diseases and pests is hampered by the lack of suitable genes. To overcome this problem we started a research program aimed at obtaining resistance by transfecting plants with genes encoding monoclonal antibodies against pathogen specific proteins. The idea is that monoclonal antibodies will inhibit the biological activity of molecules that are essential for the pathogenesis. Potato cyst nematodes are chosen as a model and it is thought that monoclonal antibodies are able to block the function of the saliva proteins of this parasite. These proteins are, among others, responsible for the induction of multinucleate transfer cells upon which the nematode feeds. It is well documented that the ability of antibodies to bind molecules is sufficient to inactivate the function of an antigen and in view of the potential of animals to synthesize antibodies to almost any molecular structure, this strategy should be feasible for a wide range of diseases and pests.Antibodies have several desirable features with regard to protein engineering. The antibody (IgG) is a Y-shaped molecule, in which the domains forming the tips of the arms bind to antigen and those forming the stem are responsible for triggering effector functions (Fc fragments) that eliminate the antigen from the animal. Domains carrying the antigen-binding loops (Fv and Fab fragments) can be used separately from the Fc fragments without loss of affinity. The antigen-binding domains can also be endowed with new properties by fusing them to toxins or enzymes. Antibody engineering is also facilitated by the Polymerase Chain Reaction (PCR). A systematic comparison of the nucleotide sequence of more than 100 antibodies revealed that not only the 3′-ends, but also the 5′-ends of the antibody genes are relatively conserved. We were able to design a small set of primers with restriction sites for forced cloning, which allowed the amplification of genes encoding antibodies specific for the saliva proteins ofGlobodera rostochiensis. Complete heavy and light chain genes as well as single chain Fv fragments (scFv), in which the variable parts of the light (VL) and heavy chain (VH) are linked by a peptide, will be transferred to potato plants. A major challenge will be to establish a correct expression of the antibody genes with regard to three dimensional folding, assembly and intracellular location. 相似文献
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35.
不同含水量菜豆种子老化过程中生理特性的研究 总被引:2,自引:0,他引:2
对不同含水量的菜豆种子( 2.1%、3.4%、3.8%、6.9%和9.0% ) 老化过程中的生理特性进行了研究。结果表明, 菜豆种子在含水量为318%时, 发芽率, 保护酶系统( SOD、POD和CAT) 活性和脱氢酶活性明显高于其它含水量的种子, 而脂氧合酶(LOX) 活性和丙二醛(MDA) 含量显著低于其它含水量的种子, 表现出较高的生活力。此结果表明适度降低菜豆种子的含水量可以提高其抗老化劣变能力;不同生活力的菜豆种子POD同工酶酶谱表现不同, 而SDS2PAGE蛋白质谱带则基本相同。 相似文献
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37.
Shohei MATSUURA Shigeru HOSHINO Hideaki HAYASHI Tetsuyuki KOHGUCHI Kyoji HAGIWARA Toshihiro OMURA 《Journal of General Plant Pathology》2002,68(1):99-102
DAS-ELISA proved to be reliable enough to detect a latent infection by Tomato spotted wilt virus (TSWV) in asymptomatic stock plants of chrysanthemum. A high density of Frankliniella occidentalis, the predominant vector, in the presence of latently infected stock plants resulted in a high incidence of disease in the chrysanthemum
production field. The incidence of disease was low when the vector thrips were not abundant in spite of the presence of latently
infected stock plants. These results suggest that an infestation of the vector thrips causes severe secondary spread of TSWV
originating from latently infected stock plants in chrysanthemum production fields.
Received 27 July 2001/ Accepted in revised form 27 November 2001 相似文献
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ZHANG Li-rong XIANG Peng XIA Wen-jie CHEN Zhen-guang ZHANG Xiu-ming LI Yan LI Shu-nong 《园艺学报》2002,18(8):896-899
AIM: To investigate the effects of PD98059 on the differentiation from mesenchymal stem cells to osteoblasts.METHODS: hMSC were separated from human marrow and expanded in cuture medium. hMSC were induced with dexamethasone, β-glycerophosphate, vitamin C which acted as osteoblast differentiation inducer. PD98059 was added into the osteoblasts induction medium. The cells were assayed with cell morphology, alkaline phosphatase (AP) activity and calcium deposition. RESULTS: The isolated cultured MSC comprised a single phenotypic population and displayed a fibroblast-like morphology. After induced with osteoblasts induction medium, the cells showed changes in cell morphology from spindle-shape to cuboidal and polygonal. The AP activity increased gradually and reached the peak in 12 days, then decreased. Many scattered tangerice calcium nodes were observed. PD 98059 significantly inhibited AP activity and calcium deposition in a dose-dependent manner. A striking observation of the present study was that a few adipocytes appeared in cultures that were treated with PD 98059 and osteogenic differentiation medium. CONCLUSION: These results indicated that osteogenic diferentiation from the hMSCs was related to the activation of the ERK. 相似文献