棉铃虫田间分布型及在实践中的应用——2.棉铃虫田间卵量调查及序贯抽样检定防治标准的方法

在调查分析了解棉铃虫卵在田间呈核心理论分布型的基础上,研究了卵量调查方法的改进,应用序贯抽样检定防治标准,及统计资料的代换方法。根据棉铃虫卵在田间呈核心分布型,为了提高田间调查准确度,改进我国已经应用的田间取样方式,提出田间卵量调查方法是:以200株为取样量,采用平行线式,40个样点,每点单行五株。因为这样增多了样点和卵分布核心相遇的机会,经与原采用的单对角线式,双对角线式,棋盘式,分行式取样比较,平行线式取样准确度最高,降低了样本平均数代表性误差。为了节省田间卵量检查时间,可采用调查着卵株率计算百株卵量的办法,或对不同卵量密度的田块区别对待,百株卵量10粒以上检查100株,10粒以下检查200株。以田间百株卵量15粒或着卵株率11.77%为达到防治标准;卵量10粒或着卵株率8.36%为未达到防治标准,制定了序贯抽样检定防治标准的方法。检查卵量序贯抽样确定防治标准的方程:d_o=0.1127n-8.5353d_1=0.1127n 8.5353检查着卵株序贯抽样确定防治标准的方程:d_o=0.0998n-6.4228d_1=0.0998n 6.4228为了使用方便,制定了每次检查五株为单位的序贯抽样确定防治标准的田间实用检定表。实例计算表明,对棉铃虫卵和田间调查资料,进行变量分析,用对数和平方根代换均可。根据卵呈核心或嵌纹分布的特点,对数代换更为合理些。     

Effect of gradual acclimation to high ambient temperatures on egg‐shell quality of the Sinai Bedouin fowl,the commercial white leghorn and their crossbreds

1. The effect of gradual acclimation to high ambient temperatures on egg‐shell quality was studied in the Sinai fowl, the commercial White Leghorn, and their reciprocal crossbreds.

2. The Leghorn egg was characterised by a thinner and weaker shell compared with the Sinai and the crossbreds, at all the experimental temperatures.

3. In contrast to other reports, high ambient temperatures for a long period had only mild effects on egg‐shell quality.

4. The results suggest that gradual acclimation to high ambient temperatures might improve the efficiency of the physiological mechanisms involved in the hen's response to heat. Consequently, the reproductive process adapts to the hot environmental conditions.

5. The results indicate that the Sinai breed might be used for future selection of a breed, highly resistant to extreme environmental conditions and with an improved shell quality.     

Pelargonium flower-break and pelargonium line pattern viruses in the Netherlands; purification,antiserum preparation,serological identification,and detection in pelargonium by ELISA

Two viruses, detected frequently in the Netherlands in pelargonium, were identified by serology and test plant reactions. Antisera were prepared and an ELISA procedure was developed to detect the viruses in pelargonium.One of the viruses, PFBV-N, proved to be pelargonium flower-break virus. With the antiserum to PFBV-N, it could be detected reliably throughout the year inPelargonium zonale Springtime Irene.The other virus, PLPV-N, was serologically closely related to pelargonium line pattern virus (PLPV) and to pelargonium ring pattern virus (PRPV), as were an old virus isolate from Saturnus, collected in the Netherlands in 1971 (L128), and PLPV isolates from Yugoslavia (PLPV-Y) and Denmark (PLPV-D). There were only minor differences in host-plant reactions between the virus isolates. Based on these tests, PLPV and PRPV are considered as isolates of the same virus, for which, for practical reasons, the name pelargonium line pattern virus is proposed.PLPV could be reliably detected by ELISA inP. zonale Springtime Irene and Amanda throughout the year with only a few exceptions. InPelargonium peltatum Tavira, however, reslts were erratic due to uneven distribution of virus in the plant. Best results were obtained when petioles of fully expanded leaves were tested.     

Detection of squash mosaic virus in seeds of melon (Cucumis melo) by enzyme-linked immunosorbent assay (ELISA)

Squash mosaic virus (SqMV, comovirus) is seed-transmitted in severalCucurbitaceae. Therefore, the use of virus-free seed is important to prevent establishment of this virus in the Netherlands and to avoid spread to other countries.This study was undertaken to develop an enzyme-linked immunosorbent assay (ELISA) for the detection of SqMV in melon seeds. An antiserum was produced to a serotype 1 isolate from melon. Two ELISA variants were investigated viz. an ELISA variant with simultaneous incubation of sample and enzyme conjugate (ELISA 1) and an ELISA variant with successive incubation of sample and enzyme conjugate (ELISA 2). The sensitivity of ELISA was tested by mixing fluor of ground infected and non-infected seeds in different proportions. SqMV was detected by both ELISA variants at dilutions of 1 160 (1 part of infected flour mixed with 159 parts of non-infected flour) or higher after a substrate incubation period of 4 h. However, ELISA 1 gave relatively higher absorbance values than ELISA 2 for nearly all dilutions. Since ELISA 1 is also faster than ELISA 2, ELISA 1 is advised for routine testing. In these test, using subsamples of 100 melon seeds SqMV is detected reliably. ELISA 1 is now used in the Netherlands for routine-indexing of melon seed lots for SqMV.Samenvatting Het pompoenemozaïekvirus gaat over met het zaad van verscheideneCucurbitaceae. Het gebruik van virusvrij zaad is belangrijk om te voorkomen dat het virus zijn intrede doet in Nederland en zich naar andere landen verspreidt.Een antiserum werd geproduceerd tegen een serotype 1 isolaat van meloen. Met behulp van dit antiserum werd een ELISA ontwikkeld om pompoenemozaïekvirus in zaden van meloen aan te tonen. Twee varianten van ELISA werden vergeleken, namelijk een variant waarbij monster en enzymconjugaat gelijktijdig geïncubeerd werden (ELISA 1) en een variant waarbij monster en enzymconjugaat na elkaar geïncubeerd werden (ELISA 2). De gevoeligheid van de ELISA varianten werd uitgetoetst door meel van zieke zaden in verschillende verhoudingen te mengen met meel van gezonde zaden. Het pompoenemozaïekvirus werd met beide ELISA varianten aangetoond in verdunningen van 1 160 (1 deel meel van zieke zaden gemengd met 159 delen meel van gezonde zaden) of hoger na 4 uur incubatie met substraat. ELISA 1 gaf doorgaans hogere extinctiewaarden dan ELISA 2 voor bijna alle verdunningen. Omdat ELISA 1 ook nog sneller is dan ELISA 2, wordt ELISA 1 aanbevolen voor routinematig gebruik. Wanneer voor routinematig gebruik 100 meloenezaden per submonster getoetst worden, kan het pompoenemozaïek virus betrouwbaar worden aangetoond. In Nederland worden momenteel per zaadpartij 20 submonsters van 100 zaden getoetst.     

Arbovirus isolations from mosquitoes in South Slovakia.

In the years 1973 and 1975 mosquitoes and some other Diptera (Tabanidae, Simuliidae, Hippoboscidae) were tested for virus. 13,924 mosquitoes, 75 horseflies and 60 blackflies were processed in 1973. Five strains of Tahyna virus were isolated from mosquito species Aedes vexans. 3,378 mosquitoes and 12 sheep keds were tested for virus in 1975. Twelve strains of Calovo virus were isolated from Anopheles maculipennis and one strain of Tahyna virus was obtained from Aedes vexans mosquitoes.     

Scanning electron microscopy of Setaria labiatopapillosa (Alessandrini, 1848) from cattle of Kazakhstan.

Surface structures of males and females of Setaria labiatopapillosa were studied by means of scanning electron microscopy at high magnification. It was found that the surface of buccal cavity, lateral appendages, vulva, anus and cloaca differs from the remaining cuticle. The shape of internal and external mouth papillae, amphids, deirids, postdeirid and phasmidial pore was studied. On the surface of the postdeirid are four small papillae arranged in a typical manner around the depression from which projects a needle-like formation. The ventral bands are formed by fine longitudinal parallel folds of the cuticle. Some anomalies in the localization of postcloacal papillae were observed.     

Nerine latent virus: some properties and serological detectability in Nerine bowdenii

Nerine latent virus (NeLV), first found inNerine bowdenii, may occur also in the otherNerine species investigated so far:N. sarniensis, N. flexuosa Alba, andN. Mansellii. Chenopodium amaranticolor, C. quinoa, andGomphrena globosa sometimes reacted with local lesions after mechanical inoculation with NeLV.Nicotiana clevelandii andHippeastrum were symptomless hosts. In this respect NeLV resembled the incompletely describedHippeastrum latent virus (HLV).Serologically NeLV was closely related to HLV and to carnation latent virus (CaLV), but differed from the latter in host plant reaction. A more distant relationship was observed with some other carlaviruses, wheareas NeLV also reacted with an antiserum to potato virus X.Depending on the lot, NeLV could be detected rather reliably with the micro-precipitin test inN. bowdenii Van Roon, but less well in 63. Better results were obtained with the microplate method of enzyme-linked immunosorbent assay (ELISA).The average particle length was 664 nm, the sedimentation coefficient 155 S and the buoyant density 1.298 g/cm³.NeLV can be considered as a member of the carlavirus group. On basis of priority HLV may be considered as NeLV.     

Phage typing of strains of Staphylococcus aureus from staphylococcal mastitis of dairy cows]

Of 62 strains of Staphylococcus aureus, isolated from milk samples of dairy-cows with staphylococcic mastitis from five agricultural enterprises, 68 p. c. could be typified as bacteriophages. In 42 typified strains, altogether 17 different phage pictures were found, in which case, in the different agricultural enterprises, the number of phage picture fluctuated between 1 and 8. Phage picture 42 D was found and dominated in all the agricultural enterprises. Of the typified strains, 76% could be ranked only into one phage group and 24% in the "M" group (= miscellaneous). Of 32 typified strains, 56.3% belonged to phage group IV, 25% to group III, 12.5% to group I, and 6.2% to group II.     

Phage typization of Staphylococcus aureus strains from staphylococcic mastitis of dairy cows]

Of 62 strains of Staphylococcus aureus, isolated from milk samples of dairy-cows with staphylococcic mastitis from five agricultural enterprises, 68 p. c. could be typified as bacteriophages. In 42 typified strains, altogether 17 different phage pictures were found, in which case, in the different agricultural enterprises, the number of phage picture fluctuated between 1 and 8. Phage picture 42 D was found and dominated in all the agricultural enterprises. Of the typified strains, 76% could be ranked only into one phage group and 24% in the "M" group (= miscellaneous). Of 32 typified strains, 56.3% belonged to phage group IV, 25% to group III, 12.5% to group I, and 6.2% to group II.     

Distribution of Cryptococcus neoformans in a pigeon habitat.

Samples of pigeon droppings were taken from 7 sites in a church tower contaminated with C. neoformans, and the distribution patterns of the fungus were studied. From various sites, 0 to 3 x 10(5) viable C. neoformans particles were recovered per one gram of dry excreta. The factors causing the different density of C. neoformans population in the habitat were: uric acid share of the total nitrogen, creatinine content, sunlight and probably pH. Chemical composition of the substrate is the primary factor in the distribution of C. neoformans in droppings.