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51.
Larsson A Dimander SO Rydzik A A Uggla Waller PJ Höglund J 《Veterinary parasitology》2007,145(1-2):129-137
A 3-year grazing trial (2002-2004) was conducted on a commercial beef cattle farm in south-central Sweden to assess different methods of parasite control. This paper focuses on the dynamics of the free-living larval stages, whereas data on performance and within-host parasitological variables are presented in a complementary paper. Each year in May, 4 groups of 10 first-season grazing (FSG) steers were turned out on to separate 2ha paddocks and subjected to the following strategies: (1) spring turn-out on to pasture which had been grazed the previous year by second-season grazing (SSG) steers (paddock RT), followed by a move to aftermath (paddock AM) after 10 weeks (mid-July), (2) supplementary feeding with concentrate and hay for 4 weeks following turn-out (paddock FD), set stocked, (3) untreated control (paddock UT), set stocked and (4) anthelmintic treated control (paddock DO), set stocked. All paddocks were assigned a new set of FSG cattle each year whereas the treatments remained the same. Pasture infectivity were monitored partly by two tracer calves that grazed each paddock along with the FSG calves for 3 weeks after turn-out and prior to housing, partly by analysis of herbage samples for infective larvae (L3) that were collected from each paddock at monthly intervals between April and October. The predominant genera found were Cooperia and Ostertagia. Tracers grazing paddock RT overall harboured less worms, and in particular less Ostertagia spp., and tracers grazing paddock AM in mid-July harboured insignificant numbers of nematodes compared to tracers on the FD and UT paddocks. Although total worm counts varied between groups, smaller numbers were generally observed early in the grazing-season (May), compared to close to housing (September) when inhibited early L4 larvae were almost exclusively found. Results observed from herbage samples showed high numbers of L3 in spring before the time of turn-out, compared to around housing. In conclusion, the rotation control strategy showed promising results and provided a turn-out pasture that was 'nematode safe' to FSG cattle the following spring, whereas the feeding strategy failed as applied in this experiment. 相似文献
52.
Elisabet Sonntag-Öström Maria Nordin Ylva Lundell Ann Dolling Urban Wiklund Marcus Karlsson Bo Carlberg Lisbeth Slunga Järvholm 《Urban Forestry & Urban Greening》2014,13(2):344-354
This experimental study investigated differences in perceived restorativeness, mood, attention capacity and physiological reactions when visiting city and forest environments. Twenty female patients diagnosed with exhaustion disorder visited three different forest environments and one city environment in randomized order. They performed a standardized 90-min test procedure in each of these environments. Evaluation of the environments and psychological effects in mood were studied with self-administered questionnaires. Attention capacity was studied with Necker Cube Pattern Control task. Physiological responses were measured with regularly scheduled controls of heart rate and blood pressure, and a single test of heart rate recovery. Visits to the forest environments were perceived as significantly more restorative, enhancing mood and attention capacity compared to the city. This also applies to the results of heart rate and to some extent to the results of the diastolic blood pressure. The results from this experimental study support our hypothesis that short visits to forest environments enhance both psychological and physiological recovery and that visits to forest environments are likely to be beneficial when suffering from exhaustion disorder. 相似文献
53.
Ylva Cecilia Bj?rnsdotter Sjunnesson Jane Margaret Morrell Raquel González 《Acta veterinaria Scandinavica》2013,55(1):20
Background
Good quality spermatozoa are important to achieve fertilization, viable embryos and offspring. Single Layer Centrifugation (SLC) through a colloid (Androcoll-P) selects good quality spermatozoa. However, it has not been established previously whether porcine spermatozoa selected by this method maintain their fertility.Methods
The semen was prepared either by SLC or by standard centrifugation (control) and used for in vitro fertilization (IVF) at oocyte:spermatozoa ratios of 1:50; 1:100 and 1:300 (or 4 x 103, 8 x 103 and 24 x 103 spermatozoa/ml) to evaluate their subsequent ability to generate blastocysts. In addition, sperm motility was assessed by computer assisted sperm motility analysis.Results
Total and progressive motility were significantly higher in sperm samples prepared by SLC compared to uncentrifuged samples. Sperm binding ability, polyspermy, cleavage and blastocyst rates were affected by the oocyte:sperm ratio, but not by sperm treatment.Conclusion
The use of SLC does not adversely affect the in vitro fertilizing and embryo-generating ability of the selected spermatozoa compared to their unselected counterparts, but further modifications in the IVF conditions would be needed to improve the monospermy in IVF systems. Since SLC did not appear to have a negative effect on sperm fertilizing ability, and may in fact select for spermatozoa with a greater potential for fertilization, an in vivo trial to determine the usefulness of this sperm preparation technique prior to artificial insemination is warranted. 相似文献54.
Tina Boddum Niels Skals Marit Wirén Robert Baur Stefan Rauscher Ylva Hillbur 《Pest management science》2009,65(8):851-856
BACKGROUND: The swede midge, Contarinia nasturtii Kieffer, is a serious pest in crucifers. Its pheromone is a blend of (2S,9S)‐diacetoxyundecane, (2S,10S)‐diacetoxyundecane and (2S)‐acetoxyundecane. The pheromone is used in monitoring traps, and this study examines possible ways to optimise the traps. RESULTS: Two dispenser types were compared: polyethylene dispensers and cotton dispensers. Polyethylene dispensers attracted male C. nasturtii for more than 6 weeks, whereas cotton dispensers were attractive for only 2 weeks. All three pheromone components were important for attraction of male midges in the field. The importance of the stereoisomeric compositions of the pheromone compounds was also tested—both in the wind tunnel and in the field. In the case of 2,9‐diacetoxyundecane and 2‐acetoxyundecane, the non‐natural stereoisomers did not inhibit male C. nasturtii attraction, whereas one or both of the stereoisomers of 2,10‐diacetoxyundecane did. CONCLUSION: Pheromone traps with the synthetic pheromone in a 1:2:0.02 ratio emitted from PE dispensers were highly effective and long lasting. As the mixture of stereoisomers of 2,10‐diacetoxyundecane strongly inhibited attraction of male C. nasturtii while those of 2,9‐diacetoxyundecane and 2‐acetoxyundecane did not have any inhibitory effect, it is possible to produce traps that are effective and long lasting but cheaper to produce and maintain. Copyright © 2009 Society of Chemical Industry 相似文献
55.
Hrabok JT Oksanen A Nieminen M Rydzik A Uggla A Waller PJ 《Veterinary parasitology》2006,136(3-4):297-306
The reindeer husbandry range of Scandinavia overlaps with sheep, goat, and cattle pastures. The aim of this study was to determine whether reindeer are suitable hosts for ovine or bovine nematode parasites, and thus may spread these parasites into the reindeer husbandry regions. To render worm-free, twelve 4-month-old male reindeer calves, six lambs, and six bovine calves were given ivermectin at 200 microg/kg body weight. Five weeks post-treatment, six reindeer calves were each artificially dosed with 10,000 third-stage larvae (L3) of gastrointestinal nematodes derived from sheep, and an additional six reindeer with L3 derived from cattle. Lambs and bovine calves received the same dose of ovine and bovine larvae as reindeer, from the same larval source, respectively. Faecal samples collected on five occasions after the larval dosing revealed that by the fourth week, all reindeer calves, lambs, and bovine calves were infected. Animals were slaughtered on days 40 (reindeer) or 47 (lambs and bovine calves) after the larval dosing. Reindeer calves were most susceptible to L3 derived from sheep. The overall mean intensity of Haemochus contortus, Trichostrongylus axei, and Teladorsagia circumcincta, did not differ between reindeer and sheep; however, early fourth-stage larvae of H. contortus were more abundant in reindeer (p = 0.002). The establishment of bovine-derived Ostertagia ostertagi was similar in reindeer (62%) and bovine calves (57%), but larval inhibition was much higher in reindeer (91%, p < 0.001) than in cattle (31%). Very poor establishment of bovine derived Cooperia oncophora was recorded in reindeer calves (2%) compared with bovine calves (59%). These results show that young reindeer are susceptible hosts to the important gastrointestinal parasites of sheep (T. circumcincta, H. contortus) and cattle (O. ostertagi), as well as being a suitable host for T. axei. 相似文献
56.
Ylva Lindgren Nils Lundeheim Sofia Boqvist Ulf Magnusson 《Acta veterinaria Scandinavica》2013,55(1):33
Background
To achieve a competitive reproductive performance in organic pig farming is a major challenge for this farming practise. Practices and research data regarding conventional pig production are not always applicable to organic production, why field studies are needed to identify differences in performance between organic and conventional pig farms in order to identify areas for improvement.Results
Performance data for one year was collected from 5 organic herds that had more than 30 sows in production and used a computerized recording system, and data from five nearby conventional farms with more than 30 sows and the same recording system were used as a comparison. In total data from 4697 farrowings were analyzed. In the organic pig herds, there were a higher total number of piglets born per litter (p=0.001), a higher number of piglets stillborn per litter (p<0.001), but a tendency (p<0.06) to lower number of weaned pigs per litter and longer nursing period (p<0.001) and farrowing interval (p<0.001).Conclusions
The reproductive performance was lower in the organic herds and the variation in reproductive performance among the organic herds was larger than among the conventional ones, suggesting options for improvement in the organic herds. 相似文献57.
Steroid hormone receptors ERα and PR characterised by immunohistochemistry in the mare adrenal gland
Ylva Hedberg Alm Sayamon Sukjumlong Hans Kindahl Anne-Marie Dalin 《Acta veterinaria Scandinavica》2009,51(1):31
Background
Sex steroid hormone receptors have been identified in the adrenal gland of rat, sheep and rhesus monkey, indicating a direct effect of sex steroids on adrenal gland function.Methods
In the present study, immunohistochemistry using two different mouse monoclonal antibodies was employed to determine the presence of oestrogen receptor alpha (ERalpha) and progesterone receptor (PR) in the mare adrenal gland. Adrenal glands from intact (n = 5) and ovariectomised (OVX) (n = 5) mares, as well as uterine tissue (n = 9), were collected after euthanasia. Three of the OVX mares were treated with a single intramuscular injection of oestradiol benzoate (2.5 mg) 18 – 22 hours prior to euthanasia and tissue collection (OVX+Oe). Uterine tissue was used as a positive control and showed positive staining for both ERalpha and PR.Results
ERalpha staining was detected in the adrenal zona glomerulosa, fasciculata and reticularis of all mare groups. Ovariectomy increased cortical ERalpha staining intensity. In OVX mares and one intact mare, positive ERalpha staining was also detected in adrenal medullary cells. PR staining of weak intensity was present in a low proportion of cells in the zona fasciculata and reticularis of all mare groups. Weak PR staining was also found in a high proportion of adrenal medullary cells. In contrast to staining in the adrenal cortex, which was always located within the cell nuclei, medullary staining for both ERalpha and PR was observed only in the cell cytoplasm.Conclusion
The present results show the presence of ERalpha in the adrenal cortex, indicating oestradiol may have a direct effect on mare adrenal function. However, further studies are needed to confirm the presence of PR as staining in the present study was only weak and/or minor. Also, any possible effect of oestradiol treatment on the levels of steroid receptors cannot be determined by the present study, as treatment time was of a too short duration. 相似文献58.
Gunnarsson L Zakrisson G Christensson D Uggla A 《Journal of the American Animal Hospital Association》2004,40(5):400-404
In a laboratory study to evaluate the efficacy of selamectin for treatment of canine nasal mite infection, 12 purpose-bred beagles were experimentally infected with Pneumonyssoides caninum (P. caninum). Six of the dogs were treated with selamectin applied to the skin of the back at dosages of 6 to 24 mg/kg for three times at 2-week intervals. The remaining six dogs were an untreated control group. At necropsy 39 to 46 days after inoculation, no P. caninum mites were found in any of the treated dogs. In contrast, nasal mites were found in five of the untreated dogs. This difference was statistically significant at P=0.015. 相似文献
59.
Blood and tissue samples obtained at necropsy from carcasses showing varying degrees of postmortem decomposition are sometimes used as sources of antibodies for serological tests, primarily in studies involving wild animals or in examination of aborted fetuses. To test the persistence of antibodies in blood and lung tissue kept at room temperature (approximately 20degreesC) for up to 48 and 118 days, respectively, serum and lung fluid from Neospora caninum-infected and uninfected cattle were tested using an indirect N. caninum immunostimulating complex enzyme-linked immunosorbent assay (ELISA). Testing of selected samples was also performed using a commercial Neospora competitive ELISA. Despite severe changes in the gross appearance of the samples, the blood collected through venipuncture from live animals remained essentially sterile, whereas the pieces of lung tissue showed a variety of bacterial and fungal growth. Nevertheless, there was no obvious reduction in antibody levels in either the blood or the tissue samples during the 2 observation periods. This study indicates that specific immunoglobulin G antibodies may be remarkably stable in sterile blood and decomposed lung tissue. 相似文献
60.
Waldenstedt L Elwinger K Lundén A Thebo P Bedford MR Uggla A 《British poultry science》2000,41(4):459-464
1. The effect of intestinal digesta viscosity on bird performance in chickens with coccidiosis was compared to those without coccidiosis. 2. Six hundred chicks were divided into five groups: one control group was fed a basal maize/soyabean-based diet and the other groups were fed the basal diet supplemented with 2, 4, 6 or 8 g carboxymethyl cellulose (CMC) per kg of feed. At 14 d of age half the birds were individually inoculated with sporulated oocysts of Eimeria acervulina and Eimeria praecox. 3. Intestinal digesta viscosity increased with increasing inclusion of CMC. This effect was considerably less pronounced in inoculated than in non-inoculated birds. 4. There was a significant negative effect on live weight gain and feed conversion ratio (FCR) with increasing CMC inclusion in non-inoculated birds, but in inoculated birds there was no clear relation between CMC inclusion and performance. Neither intestinal lesion scores, nor numbers of Clostridium pefringens in the caeca, were significantly affected by CMC inclusion. 5. Across all diets inoculation impaired growth rate by 9% and FCR by 8%, but did not affect the amount of C. perfringens in the caeca. 相似文献