全文获取类型
收费全文 | 349篇 |
免费 | 10篇 |
国内免费 | 2篇 |
专业分类
林业 | 53篇 |
农学 | 4篇 |
基础科学 | 2篇 |
37篇 | |
综合类 | 20篇 |
农作物 | 28篇 |
水产渔业 | 59篇 |
畜牧兽医 | 140篇 |
园艺 | 3篇 |
植物保护 | 15篇 |
出版年
2023年 | 1篇 |
2022年 | 9篇 |
2021年 | 7篇 |
2020年 | 2篇 |
2019年 | 9篇 |
2018年 | 5篇 |
2017年 | 9篇 |
2016年 | 6篇 |
2015年 | 12篇 |
2014年 | 18篇 |
2013年 | 16篇 |
2012年 | 22篇 |
2011年 | 22篇 |
2010年 | 12篇 |
2009年 | 8篇 |
2008年 | 36篇 |
2007年 | 12篇 |
2006年 | 29篇 |
2005年 | 25篇 |
2004年 | 15篇 |
2003年 | 31篇 |
2002年 | 17篇 |
2001年 | 8篇 |
2000年 | 12篇 |
1999年 | 1篇 |
1998年 | 5篇 |
1997年 | 2篇 |
1996年 | 3篇 |
1994年 | 1篇 |
1992年 | 1篇 |
1990年 | 2篇 |
1987年 | 1篇 |
1982年 | 2篇 |
排序方式: 共有361条查询结果,搜索用时 15 毫秒
61.
Junya ITO Tomoko YOSHIDA Yasushi KASAI Takuya WAKAI Jan B. PARYS Rafael A. FISSORE Naomi KASHIWAZAKI 《Animal Science Journal》2010,81(1):34-41
During fertilization in mammalian species, a sperm-induced intracellular Ca2+ signal ([Ca2+ ]i ) mediates both exit of meiosis and oocyte activation. Recently, we demonstrated in mouse oocytes that the phosphorylation levels of inositol 1,4,5 trisphosphate receptor type1 (IP3 R1), the channel responsible for Ca2+ release and oscillations during fertilization, changed during maturation and fertilization. Therefore, we examined the expression and phosphorylation of IP3 R1 during in vitro maturation of pig oocytes. Here, our present study shows that expression of IP3 R1 protein did not change during maturation, although the phosphorylation status of the receptor, specifically at an MPM-2 epitope, did. We found that while at the beginning of maturation IP3 R1 lacked MPM-2 immunoreactivity, it became MPM-2 reactive by 24 h and reached maximal reactivity by 36 h. Interestingly, the acquisition of MPM-2 reactivity coincided with the activation of p34cdc2 kinase and mitogen-activated protein kinase (MAPK), which are involved in meiotic progression. Following completion of maturation, inactivation of MAPK by U0126 did not affect IP3 R1 phosphorylation, although inactivation of p34cdc2 kinase by roscovitine dramatically reduced IP3 R1 phosphorylation. Neither inhibitor affected total expression of IP3 R1. Altogether, our results show that IP3 R1 undergoes dynamic phosphorylation during maturation and this might underlie the generation of oscillations at fertilization. 相似文献
62.
Yasushi Ishiguro Takahiro Asano Kayoko Otsubo Haruhisa Suga Koji Kageyama 《Journal of General Plant Pathology》2013,79(5):350-358
The objective of this study was to develop a multiplex PCR detection method for the high-temperature-growing pathogens Pythium aphanidermatum, P. helicoides and P. myriotylum. Species-specific primer pairs were designed that targeted the rDNA ITS regions. The multiplex PCR was constructed with a universal primer pair for eukaryotes directed at the 18S rDNA as a positive control, in addition to the three species-specific primer pairs. When the multiplex PCR was applied to naturally infested soils, the expected species were reliably identified, suggesting that the method is suitable for the detection of the three Pythium pathogens in environmental samples. 相似文献
63.
The aim of the present study was to provide a method for evaluating bone toxicity induced by drugs in various bones in aged rats. Male Crl:CD (SD) rats at 46 weeks of age were administered 15 mg/m(2) body surface area of doxorubicin, which effects the growth plate in weanling rats, weekly for 9 weeks by intravenous injection, and the femur, sternum, humerus and tibia were examined histopathologically. In the doxorubicin-treated group, thinning of the growth plate was remarkably observed in the proximal tibia and humerus; however, these changes were not observed in other regions. In addition, the osteoclast number per bone perimeter in the proximal tibia was significantly higher than others in control aged rat. Thus, recognizing the various histological reactions related to the time of epiphyseal closure is important for evaluating bone toxicity in aged rats. 相似文献
64.
Sthitmatee N Kataoka Y Sawada T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(11):1445-1451
A mutant strain, PBA322, was constructed by electroporation of a phagemid containing the coding region of antisense RNA of the ompH gene, encoding 39 kDa capsular protein or OmpH, into the parental strain P-1059 (serovar A:3) of Pasteurella multocida, and the pathogenicity was determined in mice and chickens. Grayish colonies of the mutant, indicating loss of capsule synthesis, were observed under a stereomicroscope using obliquely transmitted light, while iridescent colonies were observed for the parental strain. Moreover, strain PBA322 showed a low amount of OmpH compared with the parental strain on SDS-PAGE. Additionally, the capsule of strain PBA322 was thinner than that of the parental strain according to electron microscopy, correlating to the attenuation against chickens. In conclusion, strain PBA322, the mutant of P. multocida strain P-1059, was completely attenuated for chickens. 相似文献
65.
Dong F Yang Z Baldermann S Sato Y Asai T Watanabe N 《Journal of agricultural and food chemistry》2011,59(24):13131-13135
As a defense response to attacks by herbivores such as the smaller tea tortrix ( Adoxophyes honmai Yasuda), tea ( Camellia sinensis ) leaves emit numerous volatiles such as (Z)-3-hexen-1-ol, linalool, α-farnesene, benzyl nitrile, indole, nerolidol, and ocimenes in higher concentration. Attack of Kanzawa spider mites ( Tetranychus kanzawai Kishida), another major pest insect of tea crops, induced the emission of α-farnesene and ocimenes from tea leaves. The exogenous application of jasmonic acid to tea leaves induced a volatile blend that was similar, although not identical, to that induced by the smaller tea tortrix. Most of these herbivore-induced plant volatiles (HIPV) were not stored in the tea leaves but emitted after the herbivore attack. Both the adaxial and abaxial epidermal layers of tea leaves emitted blends of similar composition. Furthermore, HIPV such as α-farnesene were emitted mostly from damaged but not from undamaged leaf regions. A principal component analysis of metabolites (m/z 70-1000) in undamaged tea leaves exposed or not to HIPV suggests that external signaling via HIPV may lead to more drastic changes in the metabolite spectrum of tea leaves than internal signaling via vascular connections, although total catechin contents were slightly but not significantly increased in the external signaling via HIPV. 相似文献
66.
Ahmed Al-Busaidi Tahei Yamamoto Mitsuhiro Inoue A. Egrinya Eneji Yasushi Mori Muhammad Irshad 《Journal of plant nutrition》2013,36(7):1159-1173
ABSTRACT Soil salinity is a major abiotic factor limiting crop production but an amendment with synthetic zeolite may mitigate effects of salinity stress on plants. The objective of the study was to determine the effects of zeolite on soil properties and growth of barley irrigated with diluted seawater. Barley was raised on a sand dune soil treated with calcium type zeolite at the rate of 1 and 5% and irrigated every alternate day with seawater diluted to electrical conductivity (EC) levels of 3 and 16 dS m?1. Irrigation with 16 dS m?1 saline water significantly suppressed plant height by 25%, leaf area by 44% and dry weight by 60%. However, a substantial increase in plant biomass of salt stressed barley was observed in zeolite-amended treatments. The application of zeolite also enhanced water and salt holding capacity of soil. Post-harvest soil analysis showed high concentrations of calcium (Ca2 +), magnesium (Mg2 +), sodium (Na+), and potassium (K+) due to saline water especially in the upper soil layer but concentrations were lower in soils treated with zeolite. Zeolite application at 5% increased Ca2 + concentration in salt stressed plants; concentrations of trace elements were also increased by 19% for iron (Fe2 +) and 10% for manganese (Mn2 +). The overall results indicated that soil amendment with zeolite could effectively ameliorate salinity stress and improve nutrient balance in a sandy soil. 相似文献
67.
Le Van Chinh Kazuaki Hiramatsu Masayoshi Harada Makito Mori 《Paddy and Water Environment》2008,6(4):405-414
Chiyoda basin is located in Saga Prefecture in Kyushu Island, Japan, and lies next to the tidal compartment of the Chikugo
River to which the excess water in the basin is drained away. Chiyoda basin has a total area of about 1,100 ha and is a typical
flat and low-lying paddy-cultivated area. The main environmental issue in this basin is total nitrogen (TN) and total phosphorus
(TP) load management because TN and TP, which loaded from farmlands, degrade surface water as a result of anthropogenic eutrophication.
This paper presents a mathematical model of TN and TP runoff during an irrigation period in Chiyoda basin in order to elucidate
the pollutant fluxes that accompany water transportation in paddy fields and drainage canals, and to evaluate pollutant removal
from the study area to the Chikugo River. First, the water flow and the algorithm of gate operation were simulated by a continuous
tank model and the accuracy of the model was then evaluated by comparing the simulated water levels with observed ones during
an irrigation period. The observed and simulated water levels were in good agreement, indicating that the proposed model is
applicable for drainage and water supply analyses in flat, low-lying paddy-cultivated areas. Second, the TN and TP runoff
during an irrigation period was simulated based on the TN and TP loads that were determined by observed data in paddy fields.
For TN runoff, the simulated results and observed data were in good agreement whereas for TP runoff, the simulated results
were higher than the observed data. However, if the settled TP within the paddy tank was calculated as 6%, then the simulated
results and the observed data were in good agreement. We concluded that TN runoff from paddy field to the drainage canal system
was not affected much by the sediment related process. The present study could provide farmers and managers with a useful
tool for controlling the water distribution in an irrigation period, and the TN and TP loads in the downstream area as well
as the Chikugo River. 相似文献
68.
Yasushi Suzuki 《Journal of Forest Research》2000,5(3):201-204
The current status of wounds on residual trees was investigated and compared with the wound status from five years ago when
the stands were thinned and logged. Wound status was defined according to the four levels of severity based on appearance.
Several damaged trees were cut for inspection of the wounded areas. Most wounds of a lighter severity level had already healed
by occlusion and were no longer evident. Wounds reflecting heavy damage but had healed, were smaller in the horizontal dimension,
or width, than those not yet healed. Slight wounds, but with a current status reflecting persistent damage were smaller in
the vertical dimension, or length. An inspection of wound sections revealed the development of discoloration in the wood.
Only the slight wounds that healed early had no or small discolored areas. The width of discolored areas of wounds in which
cambium was still exposed was greater than the original width of the wound. In conclusion, wound width rather than length
significantly affects healing or occlusion of wounds. 相似文献
69.
We have previously reported that the scallop shell extract possesses free radical scavenging activity. In this study, we isolated a glycoprotein with a molecular weight of approximately 90 kDa (named 90-kDa protein) which showed 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and superoxide anion radical scavenging, reducing, and ferrous ion-chelating activities. Amino acid composition analysis showed that the 90-kDa protein was rich in Asx (Asp or Asn), Ser and Gly residues. A BLAST search of partial amino acid sequences determined by matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry revealed that the 90-kDa protein is a novel protein. The 90-kDa protein is a yellow protein that contains fluorescent substances. To the best of our knowledge, this is the first report of a radical scavenging protein from the scallop shell. 相似文献
70.
A cat with acute myeloblastic leukemia without maturation (M1) treated with combination chemotherapy
Mashita T Shimoda T Yoshioka H Takahashi Y Mitsuda M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(1):97-101
A 2-year-old domestic shorthair cat was presented to us with decreased activity and anorexia. Hematologic findings revealed a mild non-regenerative anemia, thrombocytopenia, and leukocytosis with an increase in blast cells. Bone marrow aspirates also revealed a marked increase of blasts. The blastic cells were shown to be positive for peroxidase. Acute myeloblastic leukemia without maturation (M1) was diagnosed according to the FAB classification. Chemotherapy was initiated with cyclophosphamide, vincristine, prednisolone, and cytosine arabinoside. The cat responded partially. In total, the cats were given 7 blood transfusions. The cat died 14 weeks after first being presented to us. 相似文献