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361.
Chitinase and N-acetylglucosaminidase activities in forest soils were examined by a highly sensitive method using 4-methylumbelliferyl (4MU) derivatives of N-acetyl-D-glucosamine oligomers as substrates. The method involves the fluorometric estimation of the 4-MU released through the activity of the soil enzymes when a soil sample and the substrate are incubated in a buffer. The activities of both enzymes decreased markedly with depth in the 4 forest soil profiles studied (i.e. Andosol, Podzol, 2 Cambisols). The activities of these soil enzymes were highly correlated with the carbon and nitrogen content of the soils. 相似文献
362.
363.
Akira GOTO Masaaki TAGAMI Fumiki KATO Tsukasa SUZUKI Takashi YAMAGA Harutaka MURASE Fumio SATO Munkhtuul TSOGTGEREL Tadamasa NIIKURA Tomoe MORIYAMA Akiko CHIBA Ken-ichi WATANABE Nao TSUZUKI Yasuo NAMBO 《Journal of Equine Science》2021,32(4):147
We performed a standing hand-assisted laparoscopic ovariectomy in a draft mare that presented with high serum anti-Müllerian hormone (AMH) level and had an enlarged single cystic ovary. Histopathological examination revealed no tumor cell proliferation in the ovary, but the presence of a large ovarian cyst was confirmed. In the diagnosis of abnormal ovaries in mares, a comprehensive assessment should be performed, including the monitoring of ovarian morphology and biomarkers over time, to determine the disease prognosis and treatment plan. The case of this mare with a nonneoplastic abnormal ovary and increased serum AMH level was rare. We suggest that standing hand-assisted laparoscopic ovariectomy is useful for the removal of large ovaries in draft mares. 相似文献
364.
Cistella japonica was inoculated onto the stems of youngChamaecyparis obtusa trees, and the development of external and internal symptoms was investigated for five years. Most lesions started exuding
resin from May to July during the first growing season after inoculation, while a few lesions started exuding resin during
the second growing season. Resin exudation lasted for two successive years on most lesions, and during successive three, four,
or five years on some lesions. Resin exuded excessively in the secondary phloem of the lesions and resin cysts frequently
developed there. The lesions where cambial tissue was necrosed occupied 18% of all the lesions and the resinous areas expanded
to a larger size than those where resin simply exuded in the phloem.Ci. japonica was reisolated from some inoculated lesion tissues at a high frequency. The isolation frequency of the fungus from some lesion
tissues two and more years later was zero or low and that ofCryptosporiopsis abietina was high.
This work was supported by a grant from the Forest Agency of the Japanese Government. 相似文献
365.
S Katayama M Yamanaka S Ota Y Shimizu 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1999,61(4):411-416
We have developed a new quantitative method for rabies virus (RV) detection using enzyme-linked immunosorbent assay (ELISA). The method named N-ELISA was based on the quantitation of nucleoprotein (N) in RV virions captured by RV-specific polyclonal antibodies on an ELISA plate. Both infective and defective interfering (DI) particles of RV could be detected by this method. When viruses were propagated in a medium of pH 7.4 adjusted with 7% NaHCO3, N-ELISA could detect them with titers of more than 10(6) pfu/ml, though the result did not correlate highly with that of the infectivity assay. The reason for this was considered to be that RVs included spikeless and damaged particles which were produced under conditions of low or high pH. However, in the time course of virus yield, titers of N-ELISA correlated well with those of the infectivity assay. 相似文献
366.
Sample size required for marker assisted selection in improving quantitative traits of self-fertilizing species 总被引:2,自引:0,他引:2
The efficiency of selection for desired trait genotypes in a molecular marker assisted selection for a quantitative trait
in self-fertilizing crop is considered. The QTLs controlling the trait were assumed to be unlinked. It was supposed that the
selection starts in F2, derived from a cross between inbred lines, and this selection will terminate if one or more plants with the desired trait
genotype is found. If no plant with the desired trait genotype is found in F2 then the selection is continued in the F3 progeny that is derived from a single selected F2 plant. Which F2 plant is to be selected was determined according to the rank which is related to the marker genotype of the F2 plants. And this rank was based on the expected frequency of the desired trait genotype in the progeny. The plant with the
top rank among all F2 plants is then selected with the first priority. Additionally the number of F3 plants in the progeny was set to be equal to the number of plants that are required for detecting one or more plants with
the desired trait genotype with a given probability. The probability of getting at least one plant with the desired trait
genotype is expressed as a function of the number of F2 plants (N).The required value for N and the total number of plants (T) in F2 and F3 for detecting at least one plant with the desired trait genotype were calculated for different situations. T was always smaller
for a single marker than for flanking markers. The minimum of T and monotonous decrease of N can be observed when the cumulative-expected-frequency
of selected marker genotypes of F2 plants increased.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
367.
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369.
Yasuo Suto 《Journal of Forest Research》1998,3(2):99-102
In the resinous lesion development caused by the inoculation withCistella japonica onChamaecyparis obtusa stem, numerous traumatic resin-canals were formed at certain radial intervals in the secondary phloem. These formation started
in the ring grown in the current or the previous year of inoculation and the resin-canals were subsequently formed in the
newly grown rings. The resin-canals were arranged in tangential series in one to two lines in an annual-ring. Artificially
induced resin-canal formation was similar to that in naturally infected resinous stem canker. The results from the present
study reconfirmed thatCi. japonica is the causal agent of the disease. 相似文献
370.