敌百虫溶液（水产用）中敌百虫含量测定的方法比较

对敌百虫含量两种测定方法即电位滴定法和高效液相色谱法进行比较分析。采用HPLC法测定敌百虫在2．4-3．6mg／mL范围内呈良好的线性关系（r^2=0．99993）,平均回收率为99．7％,RSD为0．5％。采用电位滴定法测定敌百虫,回收率为99．4％,RSD为0．8％。结果表明采用HPLC法,杂质分离度好,避免了氯化...     

Altered pharmacokinetics of enrofloxacin in experimental models of hepatic and renal impairment

We investigated the effects of hepatic and renal impairment on the pharmacokinetics of enrofloxacin in Sprague-Dawley rats. Experimental hepatic and renal failure were induced by carbon tetrachloride (CCL₄) and 5/6 nephrectomy, respectively. After intravenous dosing of enrofloxacin (10 mg/kg), plasma concentrations of enrofloxacin were measured using liquid chromatograph/mass spectrometry. There was no significant effect of hepatic impairment on enrofloxacin pharmacokinetics. However, renal impairment markedly prolonged elimination half life (t_1/2λz) of enrofloxacin (P < 0.05), comparing with respective control. Total body clearance (Cl_b) and volume of distribution at steady state (V_ss) were significantly decreased (P < 0.05) by renal impairment. In conclusion, these results suggested that renal impairment could affect the pharmacokinetics of enrofloxacin.     

Multiple amino acid substitutions are involved in the adaptation of H9N2 avian influenza virus to mice

To explore adaptation of avian influenza virus to mice we previously performed serial lung-to-lung passages of the influenza A/Chicken/Jiangsu/7/2002 (H9N2) strain, resulting in the isolation of a variant influenza strain lethal for mice. We now report that virulence correlates with improved growth characteristics on mammalian cells and extended tissue tropism in vivo. Sequencing of the complete genomes of the wild-type and mouse-adapted viruses revealed 25 amino acid substitutions. Some were found to reiterate known substitutions in human and swine H9N2 influenza isolates. Functions affected include nuclear localization signals and sites of protein and RNA interaction, while others are known determinants of pathogenicity and host specificity such as the viral polymerase PB2 E627K substitution. These observations suggest that enhanced growth characteristics and modified cell tropism may contribute to increased virulence in mice. We conclude that multiple amino acid substitutions are likely to be involved in the adaptation of H9N2 avian influenza virus to mice.     

大窖调制微储饲料的实用技术

新疆草地载畜能力下降,饲草不足,而农区有大量各类农作物没有很好的利用。微储饲料技术的应用,在很大程度上缓解了草料不足的问题。在压制微储饲料的生产过程中,我们对原菌液池加以了改进,对葵花头、秆可做微储原料予以肯定。     

真空井点降水联合加固软土地基的施工技术分析

根据工程实际情况,应用真空井点降水联合加固软土地基的施工方法,通过施工沉降观测和理论分析,对真空井点降水联合预压加固软土地基的施工技术及加固有效深度、有效应力的变化问题进行了研究。验证了真空井点降水联合预压加固施工方法在寒区环境的适用性,分析了在该条件下,本施工方法的加固效果和沉降变化规律,为基础设计和工程施工提供参考依据。     

农业信息化管理应用及发展

随着我国经济水平和科学技术的迅速发展,计算机技术已广泛应用于我国农业生产。把计算机技术引入到农业生产管理中,是现代科学技术发展的重要标志。将计算机技术与农业信息化管理结合在一起,能够对农业生产起重大帮助作用。信息化农业是将农业信息和农业技术知识结合在一起的一种新型农业,农业信息化可推动我国农业可持续发展。通过对计算机技术与农业信息化管理进行深入分析,提出农业信息化管理中的改进措施。      

阿鲁科尔沁旗紫花苜蓿需水规律与灌溉定额

为了给阿鲁科尔沁旗紫花苜蓿(Medicago sativa)灌溉提供科学依据,利用1984-2013年30年气象数据,采用联合国粮农组织推荐的彭曼-蒙特斯公式法,研究了阿鲁科尔沁旗紫花苜蓿需水规律和灌溉定额。结果表明,阿鲁科尔沁旗紫花苜蓿第1茬、第2茬、第3茬、第4茬、生长季、非生长季和全年需水量分别为221、187、169、179、755、70和825mm,需水强度分别为4.3、4.7、4.1、2.5、3.7、0.4和2.3 mm·d~(-1),灌溉需水量分别为194、118、66、131、508、56和564mm,灌溉定额分别为228、139、78、154、598、66和664 mm。     

盐胁迫对星星草幼苗保护酶系统的影响

在盐胁迫下,星星草幼苗过氧化氢酶活性随着胁迫时间的延长而增强,并与盐浓度呈显著正相关。超氧化物歧化酶活性随着胁迫浓度的增强而增强,当超过一定浓度(3.0%Na₂CO₃)时则趋减弱,尔后随着胁迫时间的延长而增强。过氧化物酶活性的变化规律与超氧化物歧化酶活性相似,但变化幅度较小。丙二醛含量随着胁迫时间的延长和胁迫强度的增强而增加。     

低温胁迫对草地早熟禾抗性生理生化指标的影响

研究低温胁迫对草地早熟禾生理生化指标的影响。结果表明,在5℃下,超氧化物岐化酶(SOD)和过氧化氢酶(CAT)的活性随着胁迫时间的延长而增加,当4h时出现最大值,之后活性逐渐下降。当温度为0℃与-6℃时,两种酶的活性随着胁迫时间的延长而急剧下降,接近于零,温度越低,下降越快。叶绿素和类胡萝卜素含量随着肋迫时间的延长而下降,温度越低下降越快。5℃对脯氨酸含量无明显影响,在0℃与-6℃时,随着时间的延长,含量则明显增加。5℃和0℃低温胁迫对质膜相对透性无明显影响,-6℃时,质膜相对透性明显增大。供试指标对低温胁迫敏感性的顺序是:超氧化物岐化酶、过氧化氢酶>类胡萝卜素>脯氨酸>叶绿素>质膜相对透性。