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排序方式: 共有174条查询结果,搜索用时 19 毫秒
91.
Hiroyuki Yamazaki Henki Rotinsulu Tsuyoshi Kaneko Kazuki Murakami Hiromu Fujiwara Kazuyo Ukai Michio Namikoshi 《Marine drugs》2012,10(12):2691-2697
1-Hydroxy-10-methoxy-dibenz[b, e]oxepin-6,11-dione (1) was obtained from the culture broth of a marine-derived fungus, Beauveria bassiana TPU942, isolated from a marine sponge collected at Iriomote Island in Okinawa, together with two known compounds, chrysazin (2) and globosuxanthone A (3). The structure of 1 was elucidated on the basis of its spectroscopic data (HREIMS, 1D and 2D NMR experiments including 1H–1H COSY, HMQC and HMBC spectra). Dibenz[b, e]oxepines are rare in nature, and only six natural products have been reported. Therefore, compound 1 is the seventh natural product in this class. Compounds 2 and 3 showed an antifungal activity against Candida albicans, and 3 inhibited the cell growth against two human cancer cell lines, HCT-15 (colon) and Jurkat (T-cell lymphoma). Compound 1 did not show an apparent activity in the same bioassays. 相似文献
92.
Lysyl oxidase (LOX) plays a critical role in the formation of cross-linkages in extracellular matrix molecules. Thus, it is essential for the biogenesis and homeostasis of the connective tissue matrix. During development, collagen fibres and elastic system fibres emerge and accumulate in a temporospatial manner in the presumptive dermis of chicks. In this study, we investigated LOX mRNA expression by laser capture microdissection and RT-qPCR and LOX protein localization by immunohistochemistry. The picrosirius polarization method was used to investigate a relation between collagen accumulation and LOX expression. PCR analysis showed that the expression of LOX mRNA in the presumptive dermis became apparent at embryonic day 13 and increased considerably by ED17. Immunohistochemical staining for LOX in the dermis was very low at all stages of development. Accumulation of collagen fibres was seen in the dermis on ED10, and higher wavelengths of birefringence became evident by ED13. Our findings suggest that the temporal pattern of LOX mRNA expression correlates with collagen fibre accumulation in the dermis of the developing chick limb bud, whereas LOX expression was relatively constant at the protein level. 相似文献
93.
Kenshi Katahira Miho Ishitake Hiroshi Moriwaki Osamu Yamamoto Tadao Fujita Hideo Yamazaki Shusaku Yoshikawa 《Water, air, and soil pollution》2009,204(1-4):215-225
Metal concentrations in a sediment core from a moat outside Osaka Castle in Japan were measured by ICP-MS following a microwave extraction method. Concentrations of metals in the sediment core samples peaked around 1945 when the World War II ended. This is in part due to great air raids on Osaka. In cluster analysis using metal concentrations, the fractions of sediment core samples were classified into two groups, representing natural sources (1795–1915) and anthropogenic sources (1922–1976), respectively. Results of lead isotope ratios also showed the anthropogenic influences from 1915 by changing the ratio values compared to those of natural sources. In addition, several components contributing to metal concentrations were identified by principal component analysis. The main component was controlled by natural sources and a decrease of the component score corresponded to an increased influence from anthropogenic sources. The largest impact of anthropogenic sources was shown around 1945. 相似文献
94.
A decline in the hot-water-soluble pectin and an increase in the hot-water-insoluble pectin were observed when cucumber fruits were subjected to chilling temperatures. Infrared absorption spectra revealed the presence of highly esterified carboxyl groups in the soluble pectin, and of free carboxyl groups in the insoluble pectin. An increase of the insoluble pectin during chilling was also found in other chilling-sensitive plants. From these results it is suggested that a de-esterification of pectin and the concomitant increase of polymeric pectin takes place during chilling, making cell walls firmer, and that these pectic changes may be a characteristic common to a number of chilling-sensitive plants. 相似文献
95.
Yamazaki K Teraoka H Dong W Stegeman JJ Hiraga T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(9):829-833
Cytochrome P450 1A (CYP1A) is well known for being induced by aromatic hydrocarbons, including 2,3,7,8-tetrachloro-dibenzo-p-dioxin (TCDD). We determined the complete cDNA sequence of a CYP1A open reading frame with both 5'- and 3'-ends in zebrafish (zfCYP1A), a useful model for environmental toxicology. zfCYP1A shows high percentage identity with CYP1As of mammals, domestic fowl and xenopus (51.9-60.4%), as well as the other fish species (63.8-89.2%). As revealed by in situ hybridization and immunohistochemistry, zfCYP1A was scarcely detected in control embryos but was markedly induced by TCDD especially in heart, vascular endothelial cells, intestinal epithelium, pronephros and outer integument in both prehatched and hatched embryos. These expression patterns are consistent with possible involvement of zfCYP1A in TCDD-induced toxicities. 相似文献
96.
Barbara Glanemann Karen Humm Mariana Abreu Sophie Aspinall David Buckeridge Hope Carveth Hannah Darcy Jessica Florey Polly Frowde Isuru Gajanayake Kate Green Emma Holmes Alenka Hrovat Anne-Katherine Jasensky Bryn A Jones Vasiliki Lantzaki Eve JY Lo Kirsty MacDonald Kevin O'Brien Alejandro Suárez-Bonnet Nele Van den Steen Balazs Szladovits Annelies Willems Helen Wilson 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2023,37(1):126-132
97.
Hiromichi Yamazaki Sunao Kikuchi Tsuguo Hoshina Takeshi Kimura 《Soil Science and Plant Nutrition》2013,59(2):529-534
Bacterial wilt of tomato (Lycopersicon esculentum Mill.) caused by Ralstonia solanacearum Smith is a serious disease in Japan. We previously reported that calcium (Ca) nutrition in tomato significantly affected the resistance to the disease, and that highly resistant cultivars were characterized by a high Ca uptake. We examined the relationship between the Ca uptake and resistance using mutually grafted seedlings of tomato cultivars differing in their resistance. A susceptible (‘Ponderosa’) or moderately resistant (‘Zuiei’) cultivar (scion) was grafted on the rootstock of a susceptible, moderately resistant, or highly resistant cultivar (‘Hawaii 7998’). Roots or petioles of the grafted seedlings were inoculated with the pathogen, and the development of bacterial wilt was observed. Although Ca uptake by shoot increased by grafting on the rootstock of a highly resistant cultivar, the development of the disease was not influenced by the difference in Ca uptake, and depended on the resistance of the cultivar to which the inoculated part of the graft belonged. It was concluded that the differences in Ca uptake of the shoot of the grafted tomato seedlings might not be related to the expression of the resistance to bacterial wilt. 相似文献
98.
Masatsugu Yamazaki Taisuke Ohtsuka Yasushi Kusuoka Masayoshi Maehata Hiroyuki Obayashi Kiyoyuki Imai Fujiyoshi Shibahara Makoto Kimura 《Fisheries Science》2010,76(2):207-217
We examined the effects of predation by Nigorobuna Carassius auratus grandoculis larvae and fry, a crucian carp endemic to Lake Biwa, Japan, on the community structure of aquatic organisms in rice fields.
Six experimental plots with three different rice-straw treatments in the presence/absence of stocked larvae were prepared.
In each plot, the number of aquatic organisms ranging in size from 30 μm to 5 mm in the water, as well as those from 63 μm
to 5 mm in size in the surface sediments, was surveyed 6, 13, 20, 26, 34, and 41 days after the onset of irrigation. Three-day-old
fish larvae were released on day 10. Undigested organisms in the gut contents of the larvae or fry were identified on days
20, 26, 34, and 41, respectively. Ten-day-old larvae mainly preyed on Cladocera, but the fry thereafter shifted to Diptera
as their main prey. While Cladocera and Podocopida decreased in fish-stocked plots, Euglenales and Halteriida became more
abundant there. Top-down or bottom-up effects of fish seemed to control these changes in community structure. 相似文献
99.
Bagenda Dominic Kasujja Yamazaki Koji Kobayashi Tetsuya Kawai Yuji 《Fisheries Science》2010,76(2):395-401
The compatibility and potential of nisin, a Pediococcus pentosaceus fermentate, and pectin to inhibit Listeria monocytogenes in soy-seasoned salmon roe at 5 and 12°C was studied. The compatibility of nisin and soy-seasoning was assessed by adding
6.3 μg/g of nisin to freshly washed or soy-seasoned (60% soy, 15% sweet-sake, 5% sugar, and 15% water) roe and monitoring
the growth of an inoculum of L. monocytogenes (5.6 Log CFU/g; strains IID 578, IID 581, ATCC 7644) as well as residual nisin concentrations at 12°C. The combined antimicrobial
potential was determined by monitoring growth of the inoculum (5.2 Log CFU/g) in roe when the soy-seasoning contained nisin
(0.05 g/ml), fermentate powder made by freeze-drying a broth culture of P. pentosaceus (0.1 g/ml), and 2% pectin at 5 or 12°C. Chloride content, total acid content, and the effect of pectin on acceptability of
roe were also determined. Nisin was more effective in soy-seasoned roe than in freshly washed roe. Microbial growth was completely
inhibited by a combination of soy-seasoning, nisin, the fermentate, and pectin. Despite its viscosity, pectin did not significantly
affect the chemical properties or acceptability of roe relative to pectin-free roe samples (P < 0.05). Based on these results, we conclude that nisin’s potential as an antimicrobial agent in soy-seasoned roe can be
enhanced using fermentates and pectin. 相似文献
100.
To fill in the gaps in knowledge as to how individual sea cucumber Apostichopus japonicus behave consuming eukaryotic food sources in natural environments, eukaryotic communities in the faeces of sea cucumbers and sediments were analysed through one whole year based on 16S rRNA gene sequencing of the organelle genomes. A total of 390 eukaryotic features were obtained, and 99.7% of the features were assigned to chloroplasts. The eukaryotic communities in faeces and sediments showed seasonal fluctuations through one whole year based on Bray‐Curtis distance and community composition. Comparison of eukaryotic communities between faeces and sediments showed that 12 families including Chaetocerotaceae and Laminariaceae were more abundant in faeces than in sediments, suggesting that sea cucumbers may choose sediment containing these algal taxa more often compared with others in natural environments. All features of Laminariaceae were assigned to Saccharina japonica, which is consistent with the fact that this alga is one of the most suitable diets in the aquaculture of A. japonicus. Assessments of individual 16S amplicon sequences of both faecal and sediment samples could be an alternative tool to help us understand dynamic feeding behaviours of sea cucumber populations in contributing to bioresource conservation and development of a superior approach to aquaculture. 相似文献