Effect of Temperature and Alkaline pH on the Physicochemical Properties of the Protein Isolates Extracted from the Whole Ungutted Lanternfish (Benthosema pterotum)

The aim of this study was to evaluate functional properties of the protein extracted from lanternfish (Benthosema pterotum). Extraction of the fish protein was performed in alkaline pHs (10 and 12) followed by precipitation at its isoelectric pH. The effects of different extraction temperatures (0, 4, 11, 25, and 30°C) were also studied. The results showed that the protein recovery yield was higher when extracted at pH 12 compared to pH 10. Furthermore, some functional properties including water holding capacity, oil holding capacity, emulsifying capacity, foaming capacity, and solubility of the protein isolated at pH 12 were higher than that isolated at pH 10 (p < 0.05). The color evaluation (L*, a*, and b*) of the samples showed protein isolated at pH 12 was lighter (higher L*) than protein isolated at pH 10, but redness (a* value) declined in both samples. Evaluation of different extraction temperatures showed that protein recovery yield and functional properties were improved with increasing temperature. Fish protein isolate can be used in the formulation of value-added products because of their distinctive functional properties.     

Synthesis and characterization of new copolymers from glycidyl methacrylate and tetrahydrofurfuryl acrylate: Determination of reactivity ratios

The new copolymers from different feed compositions of glycidyl methacrylate (GMA) and tetrahydrofurfuryl acrylate (THFA) were synthesized using free radical polymerization in toluene at 70±1 °C using benzoyl peroxide (BPO) as initiator. The polymers were characterized by ¹H NMR, ¹³C NMR and FTIR spectroscopic techniques. The polydispersities of the copolymers suggest a strong tendency for chain termination by disproportionation. The glass transition temperature of the copolymers increases with increase in GMA content. The thermal stability of the copolymers increases with increase in THFA content. The copolymer compositions were determined using ¹H NMR analysis. Reactivity ratios for GMA and THFA as determined by the Mao-Huglin method were r₁=0.379 and r₂=0.266. The results showed that all these copolymerizations are strictly linear systems describable by the Mayo-Lewis equation based on the terminal model and that accurate reactivity ratio data can be obtained.     

Plant and soil fertility characteristics of different winter wheat fields in the Huang-huai-hai plain of China

High-profile nutrient accumulation has caused low nutrient use efficiency and environmental pollution. A total of 140 points in farmers’ wheat fields were investigated and sampled under a typical winter wheat and summer maize rotation system in the Huang-huai-hai Plain of China (HPC). The average wheat yield in the high-yield field is more than 10% relative to the mid-low-yield areas based on historical data. The results showed in the high-yield wheat field the effective spike number was the main yield component, followed by 1000-grain weight. Uptake contents and ratios of N, P₂O₅ and K₂O in the whole wheat plant were also examined. Along the soil depth, organic matter (OM), alkali-hydrolyzable N (AN), available P (AP) and available K (AK) concentration gradually decreased. Soil critical values of AN, AP and AK in the 0–20?cm depth needed to achieve the maximum grain yield were 85.9, 17.7 and 114.2?mg kg^?1 calculated by the significant linear-plus-plateau model between wheat grain yield and soil nutrient concentration at harvest, respectively. These results indicated that the rational application of fertilizer based on the soil nutrient supply capacity and residual nutrients in root zone and optimum planting density are key determinants of grain production in the HPC.     

Pit and mound influence on soil features in an Oriental Beech (Fagus orientalis Lipsky) forest

Windthrow, i.e. the felling of trees by wind, occurs continually in forest ecosystems. The uprooting of old trees creates multiple microsites (e.g. pit and mound landscape) that are the main source of soil heterogeneity. To determine the impact of pit and mound landscapes on soil features, a beech forest of the Langa district (Mazandaran province, Northern Iran) was studied. An area of 25 ha was considered for this study wherein three microsites were distinguished, including pit bottom (pit), mount top (mound) and level areas (closed canopy). In this area, 22 uprooted trees were also found. Soil samples were collected at different depths (i.e. 0–15, 15–30 and 30–45 cm) from all microsites and analysed. Our study shows that windthrow events should be considered as an important factor in influencing forest ecosystem, as they affect physical (i.e. density, texture and water content), chemical (i.e. pH, organic C, total N, cation exchange capacity and available nutrients) and biological (i.e. soil microbial respiration and earthworm density/biomass) characteristics of soil, thus resulting in pit and mound microsites that may strongly differ with respect to the closed canopy.     

Field-specific variable rate fertilizer application based on rice growth diagnosis and soil testing for high quality rice production

This study was carried out to verify the applicability of variable rate fertilization (VRF) based on soil testing and diagnosis of rice plant growth for high quality rice production of var. Chucheongbyeo at the farm level. The field trials were conducted at Icheon in Gyeonggi province on a 10 ha farm consisting of 45 experimental fields. For comparative study, 15 field trials were carried out adopting fertilizer management (FPM) practices currently used by farmers. FPM fields were managed by each rice grower using current cultivation methods, but in each VRF field fertilizer application was prescribed using soil test results and the amount of N fertilizer for top-dressing at panicle initiation stage was calculated using rice growth value at that stage. In VRF fields, the total amount of N fertilizer application was less (72 kg ha⁻¹) than that in FPM fields (103 kg ha⁻¹). However, the amount of K₂O fertilizer application was more in VRF fields (60 kg ha⁻¹) than that in FPM fields (52 kg ha⁻¹). The amount of P₂O₅ fertilizer application was similar between the VRF and FPM fields. Plant height was significantly shorter and the number of tillers was significantly more at VRF fields than at the FPM fields. Coefficient of variation (CV) of each growth characteristic measured in VRF was lower than that of FPM fields at panicle initiation stage. There was no difference in culm and panicle length and panicle number between them at the grain filling stage, but CV of panicle numbers per m² decreased in VRF compared with that of the FPM fields. Rice yield was not different between VRF and FPM fields despite higher brown rice recovery and 1,000-grain weight in VRF fields. Under VRF management, head rice yield increased due to an increase in head rice ratio accompanied by a reduction in brown rice protein content and variation of quality characteristics. These results suggest that VRF application based on soil tests and measurement of rice growth value at panicle initiation stage has the potential for quality control and production of high quality rice through increasing uniformity of growth and reducing the variability in quality among individual fields.     

Barley growth,yield, antioxidant enzymes,and ion accumulation affected by PGRs under salinity stress conditions

Application of plant growth regulator (PGR) may alleviate some negative effects of environmental stresses such as salinity. A controlled environment experiment was conducted to study barley (Hordeum vulgare L. cv. Reyhane) growth, yield, antioxidant enzymes and ions accumulation affected by PGRs under salinity stress conditions at Shiraz University during 2012. The treatments were PGRs at four levels—water (as control), cycocel (CCC, 19 mM), salicylic acid (SA, 1 mM), and jasmonic acid (JA, 0.5 mM)—and four salinity levels—no stress (0.67 dS m^?1, as control), 5, 10, and 15 dS m^?1, which were arranged in a factorial experiment based on completely randomized design with four replicates. The results showed that salinity stress significantly decreased plant height, peduncle length, leaf area, ear length, grain number, dry weight, grain yield, harvest index, potassium (K⁺) accumulation, and potassium/sodium (K⁺/Na⁺) concentration ratio, which were closely associated with stress severity. However, PGRs compensated some of these negative effects, so that SA foliar application had the most ameliorative effect. Salt stress also increased Na⁺ accumulation as well as the activity of peroxidase, catalase, and superoxide dismutase (SOD). Since ion discrimination and enhanced antioxidant enzymes are associated with salt tolerance, in this experiment PGRs application might have enhanced K⁺ accumulation and antioxidant enzyme activity. The activity of SOD and K⁺/Na⁺ ratio were found to be useful in salt tolerance manipulation in barley plants.     

Human energy expenditure in lowland rice cultivation in Malaysia

A study was undertaken to evaluate the human energy consumption of various field operations involved in lowland rice cultivation in Malaysia. Based on recorded average heart rates, fertilizing was found to be the most strenuous operation, with an average heart rate of 138 beats min(-1). There were no significant differences in the average heart rates of the subjects among the individual tasks within the first plowing, second plowing, and harvesting operations, with the average heart rates for these three tasks being 116, 106, and 106 beats min(-1), respectively. The corresponding energy expenditures were 3.90, 3.43, and 3.35 kcal min(-1). Loading the seed into the blower tank and broadcasting the seed were the most critical tasks for the seed broadcasting operation, with average heart rates of 124 and 136 beats min(-1), respectively. The highest energy expenditure of 418.38 kcal ha(-1) was observed for seed broadcasting, and the lowest energy expenditure of 127.96 kcal ha(-1) was for second plowing. The total seasonal human energy expenditure for rice cultivation was estimated to be 5810.71 kcal ha(-1), 55.7% of which was spent on pesticide spraying. Although the sample size in this study was relatively small, the results indicated that human energy expenditure per unit area (kcal ha(-1)) was positively linked to the average heart rate of the subjects and negatively linked to the field capacity. Thus, mechanization of certain tasks could decrease worker physical effort and fatigue and increase production.     

Standardization of the outbred BALB/c mice as a suitable animal model for <Emphasis Type="BoldItalic">Besnoitia caprae</Emphasis> studies

The Apicomplexan parasite Besnoitia caprae infects wild and domestic goats. Knowledge on Besnoitia caprae specially an optimized animal model is sparse. Experimental infections with tachyzoites of BC-Pars obtained from BALB/c mice were conducted in outbred mice to determine the infectivity and LD50 of Besnoitia caprae. Six groups of five mice were intraperitoneally infected with 12.5 × 10³, 25 × 10³, 5 × 10⁴, 1 × 10⁵ and 2 × 10⁵ tachyzoites and a control inoculum of DMEM, respectively. Although morbidity and mortality were observed in all groups, two mice in the 12.5 × 10³ group showed alopecia and skin lesions on 60 days post-infection (DPI). Histopathological and molecular examination of skins confirmed B. caprae infection. The LD₅₀ was calculated as 25 × 10^3.2 tachyzoites per mouse. The results indicate that outbred BALB/c mice can be used as a suitable model of besnoitiosis and to screen candidate treatments and to test the efficacy of vaccines for besnoitiosis.     

Prevalence of Brucellosis in Horse North-East of Iran

Brucella preferentially infects cattle, swine, sheep, and goats. However, some epidemiological surveys have been carried out to investigate nonruminants, such as horses. Horse brucellosis has been found in clinical cases, but there are few epidemiologic patterns. Between May 2008 and April 2009, a total of 120 horses were screened for brucella infections in Mashhad, Iran, by the rose bengal test and the tube agglutination test. Sera from three horses were found positive by rose bengal test and tube agglutination test, and therefore the prevalence rate was 2.5%. In horses, the highest individual seroprevalence was in an animal kept close under the intensive system, with other animals such as cattle, sheep, and goats. The zoonotic aspects of brucellosis from the horse must, therefore, be considered because the disease is important from a public health standpoint. The present study documents the first serological evidence of Brucella spp. infection in horses in Iran.     

Downed logs improve soil properties in old-growth temperate forests of northern Iran

Dead trees, particularly downed logs, play an important role in the dynamics of forest ecosystem. Contribution of decaying wood to C and nutrient pools of forest soils depends on the tree species and degree of wood decay. However, the extent to which the downed logs affect the soil properties of temperate forests has rarely been evaluated. In this study, a mixed beech forest was selected in Liresar region of Mazandaran Province, northern Iran, to investigate if and how the presence of downed logs affected soil quality and function by comparing soils underneath degraded logs and nearby soils of the two dominant tree species(beech and hornbeam). We then explored how these effects occurred as downed logs decomposed by comparing the woods of both tree species at four degrees of decomposition. Degree of decay of downed logs was classified into four classes(DC1–DC4). Eight dead trees of each tree species were selected at the center of each sample plot. Three composite soil samples underneath each decaying log and 100 cm away from a decaying log were collected at two soil depths(0–15 and 15–30 cm) to analyze soil main physicochemical properties and microbial activity. The results revealed that downed logs affected soil physical(5% wetter than control soils), chemical(2% lower pH, 100% increase in organic C and total N in the case of hornbeam, and 2% increase in P), and biological characteristics(soil microbial respiration enhanced by 10%, and microbial biomass C 620 and 351.5 mg kg~(-1) and microbial biomass N 66.47 and 32.18 mg kg~(-1), respectively, in the cases of beech and hornbeam), thus resulting in significantly different soil microsites from those without downed logs. Presence of downed logs increased soil microbial activity and soil fertility as wood decayed. Thus, the presence of downed logs is an important factor influencing forest soils and should be taken into consideration in forest management practices.