Osteological development and anomalies in larval stage of hatchery‐reared yellow catfish Pelteobagrus fulvidraco

Comprehensive knowledge of osteological development of fish not only provides means for understanding its functional development, but also allows early detection of skeletal anomalies. The present study was conducted to determine osteological development and occurrence of anomalies from the first to the 40th day after hatching (DAH) for yellow catfish Pelteobagrus fulvidraco, a commercially important species in China. Vertebral ontogeny started with formation of anterior neural arches at 3 DAH, and completed at 6 DAH. Vertebral centra started to develop at 4 DAH and ossification in all centra was visible at 15 DAH. Caudal fin appeared at 2 DAH and ossification was visible at 26 DAH. The onset of dorsal and anal fins appeared at 4 DAH and their ossifications were visible at 20 DAH. Pectoral fins were present before first feeding and formed entirely at 9 DAH, and their ossifications were visible at 21 DAH. Pelvic fins appeared at 9 DAH, and formed completely at 21 DAH. The ossification of pelvic fins was not finished until the end of the experiment (40 DAH). 24 types of skeletal anomalies were observed. About 20% of individuals showed at least one anomaly. Haemal vertebrae anomalies occurred at the highest frequency, followed by pre‐haemal, caudal vertebrae and anal fin anomalies, and caudal fin had the lowest anomalies frequency. For the first time, our study determined osteological development and anomalies incidence in larval yellow catfish, which help further investigations into rearing conditions leading to appearance of these anomalies to prevent their incidence.     

高效液相色谱法测定生物胺衍生条件的优化研究

利用荧光检测器（FLD）,采用柱前衍生高效液相色谱法（HPLC）对组胺（HIS）、色胺（TRP）、2-苯乙胺（2-PHE）、腐胺（PUT）、尸胺（CAD）、酪胺（TYR）、亚精胺（SPD）和精胺（SPM）8种生物胺（BA）进行测定分析。通过探究丹酰氯（Dns-Cl）质量浓度、反应体系的pH、衍生时间及衍生温度对生物胺衍生反应的影响,确定最优的衍生条件。结果显示,当Dns-Cl的质量浓度为BA质量浓度的10倍、pH 11.0、40℃下避光反应45 min,所有BA均能有效分离。BA的相对标准偏差（RSD）在3%以内,能够满足分析要求。     

青海蒲公英属植物的经济价值及开发利用前景

蒲公英属植物是青海高原的一种重要野生植物资源.本文论述了蒲公英的营养价值、药用价值、生态价值及开发应用前景.     

胰岛素、胰高血糖素对体外培养肝细胞DGAT2mRNA的影响

为阐明神经内分泌因子胰岛素、胰高血糖素在奶牛脂肪代谢过程中的调控作用,用荧光定量PCR方法检测胰岛素、胰高血糖素对肝细胞二酰基甘油酰基转移酶（DGAT2）mRNA丰度的影响。结果表明低浓度的胰岛素能促进脂肝细胞内DGAT2mRNA的表达;胰高血糖素抑制脂肝细胞内DGAT2mRNA表达。由此证明：胰岛素和胰高血糖素能直接调控肝细胞中的DGAT2基因mRNA的表达。     

天津重点水域底栖生物调查分析

于2018年9月,对天津大港,宝坻,华苑等地区的12个站点的大型底栖生物的群落结构及生物多样性进行了调查分析。结果表明,调查区共鉴定出大型底栖生物3门10种,其中软体动物4种,环节动物4种,水生昆虫2种,各站位丰度波动范围为20～840 ind/m^2,平均为205.33 ind/m^2,生物量范围为0.012～308.63 g/m^2,平均为59.69 g/m^2;囊螺为该地区绝对优势种。丰富度指数Margalefs.index在1.48～3.334之间波动, Goodnight修正指数在0.523～1之间波动。     

The loss of genetic diversity in Dabry's sturgeon (Acipenser dabryanus,Dumeril) as revealed by DNA fingerprinting

• 1. Dabry's sturgeon, a large, long‐lived migratory fish is endemic to the Yangtze River. Over‐fishing and habitat destruction have caused large‐scale declines in natural stocks in the last two decades.
• 2. Examining patterns of genetic diversity has become an integral component of many management plans for endangered species. DNA fingerprinting was applied to detect genetic diversity in Dabry's sturgeon collected in 1958–1959, 1980–1981 and 1998–1999.
• 3. Studies on direct genetic parameters (genetic variability, hypervariable loci and heterozygosity) and indirect parameters (band‐sharing coefficient and allelic frequency) showed that the continuous decline in wild populations has caused the loss of genetic diversity in present‐day sturgeon.
• 4. The present‐day populations have the lowest genetic variability; thus, effective management is needed to preserve genetic diversity.
• 5. A conservation strategy is urgently required, comprising artificial rearing facilities coupled with breeding management plans.

Copyright © 2003 John Wiley & Sons, Ltd.     

Study of electrization of powder skim milk during milk particle movement

Electrization of particles of powder materials is a widely spread phenomenon that makes a practical impact on the functional efficiency of equipment designed for producing a broad range of processed disperse products (dry milk, flour, grain, etc.). The results of electrization tests of powder skim milk particles are presented. A mathematical model of inner and outer transfer of electric charge for a single powder skim milk particle is suggested. The studies on a specialized laboratory unit have made it possible to obtain data on triboelectric charging of particles in different testing conditions.     

光诱鱿鱼浮拖网渔具渔法试验

首先对诱鱼光场进行计算，并分析其对光诱鱿鱼浮拖网作业的影响，据此合理配置了灯光强度及其布局，探讨了一套较有效的诱导渔方法。根据鱿鱼的趋光习性及浮拖网的特点，设计了适应在较深渔场作业的光诱浮拖网渔具，并成功地试验了光诱鱿鱼浮拖网柔性网板；取得了良好的社会生态效益和经济效益。     

Annual Energy Consumption and Feasibility Assessment of Ground -Source Heat Pump in Residential Building in Fuzhou City

This paper describes the annual energy consumption and economic assessment of air-source heat pump(ASHP) and ground-source heat pump(GSHP),based on hourly building load of typical house in Fuzhou.Analysis results show that the energy consumption of GSHP is 15% less than that of ASHP through all year,where there is 13% less in summer and 20% less in winter.Although the seasonal energy efficiency ratio of GSHP in Fuzhou is higher than that of ASHP,it has no obvious effect on the energy efficiency as it is in the hot summer and cold winter zone.In comparison with ASHP,the GSHP is not cost-effective.     

基于多时相GF-1WFV 数据的南方丘陵地区甘蔗种植面积提取

南方丘陵地区甘蔗种植具有分散、形状多样等特点,利用中低分辨率遥感数据提取甘蔗种植面积时,数据的有效性和精度都难以满足要求.利用高分辨率GF-1WFV遥感数据,分析比较甘蔗与其他地物类的光谱特征、纹理特征及植被指数时间变化差异,采用多时相迭代方法构建甘蔗提取特征向量决策树模型.利用该模型提取了广西江州区的甘蔗种植面积,经野外实地调查验证,甘蔗面积提取的用户精度为90.13％,生产精度为88.78％,表明GF-1WFV数据是复杂地形下提取甘蔗等农作物的潜力数据源,该提取思路可以为在全国范围内不同地区了解甘蔗种植情况,进行甘蔗长势监测和产量估测提供技术参考.