Green tea polyphenols supplementation alters immunometabolism and oxidative stress in dairy cows with hyperketonemia

Peripartal cows often experience negative energy balance, and are therefore prone to suffering from metabolic diseases such as hyperketonemia, which causes financial losses in dairy farms. This study aimed to investigate the effect of green tea polyphenol (GTP) supplementation during the periparturient period on production performance, oxidative stress and immunometabolism in dairy cows with hyperketonemia. One hundred Holstein cows were assigned to GTP (0.2 g/kg DM; n = 50) or control (without GTP; n = 50) group based on body weight, previous milk yield, and parity on d 15 before expected parturition. Subsequently, 10 cows with hyperketonemia were selected from each group, according to blood β-hydroxybutyric acid (BHBA) concentration between 1.2 and 2.9 mmol/L from 2 to 3 d postpartum. All cows were fed a close-up diet and a lactation diet with or without GTP supply from 15 d prepartum until 30 d postpartum. Milk and blood samples were obtained from 20 cows selected with hyperketonemia on 10, 20, and 30 d postpartum. Compared with control cows, greater milk yield and lower somatic cell count were observed in GTP cows. The GTP group had lower concentrations of BHBA, free fatty acids, cholesterol, triglyceride, reactive oxygen species, malondialdehyde, and hydrogen peroxide, greater concentrations of glucose, lower activities of aspartate aminotransferase, alanine aminotransferase, and glutamyl transpeptidase, alongside greater activities of superoxide dismutase, glutathione peroxidase, and total antioxidant capacity. Additionally, GTP supplementation up-regulated concentrations of interleukin-6 and interleukin-10, but down-regulated concentrations of tumor necrosis factor-α, interleukin-1β, interleukin-2, interleukin-8, and interferon-γ in plasma. Greater concentrations of plasma immunoglobulin G were also detected in the GTP group. Overall, the data suggested that GTP supplementation from 15 d prepartum to 30 d postpartum improved the milk yield and health status in cows with hyperketonemia during early lactation.     

育种咨询系统中的水稻杂交后代性状预测

杂交育种是一个很复杂的组合过程。由于指导育种的遗传学原理还远不够完善,因此,在实践中更多的是依赖育种家的经验和技巧,从而造成了育种工作中一定程度上的盲目性。为了提高育种效率,有必要发展一套为育种工作服务的咨询系统。利用它协助育种家收集数据、分析育种过程。本文重点阐述水稻杂交咨询系统研制中的杂交后代性状预测方法及原理。     

苹果果实表皮总蛋白提取及双向电泳方法的优化

以苹果果实表皮为试材,采用3种方法提取果实表皮总蛋白,并对双向电泳(2-DE)操作体系进行优化。结果表明:改良酚提法适于苹果果实表皮总蛋白的提取,优化后的2-DE分离体系为预制IPG胶条18cm pH 4~7,上样量600μg,改进后的等电聚焦程序,浓度16%的分离胶,适于苹果果实表皮总蛋白的2-DE分离。     

外源乙烯和1-甲基环丙烯处理对采后胡萝卜品质的影响

以胡萝卜为试材,采用乙烯及乙烯受体抑制剂(1-甲基环丙烯)处理,通过对贮藏60d后胡萝卜的品质劣变率(长须根率、腐烂指数、出薹率)、外观指数、可溶性糖含量及多酚氧化酶活性进行测定,研究了1-MCP对胡萝卜采后贮藏品质的影响。结果表明:1-MCP结合手洗贮藏可抑制胡萝卜贮藏期间的品质劣变率、抑制可溶性糖含量的损失、保持较高的外观指数及抑制多酚氧化酶(PPO)活性。手洗后贮藏则可有效改善胡萝卜贮藏期间的表皮褐变问题。     

喷漆机器人喷枪轨迹离线优化方法

介绍了喷漆机器人喷枪轨迹离线优化方法问题的数学模型，建立了评价喷涂效果的目标泛函，确定了具体的算法．并以圆锥面工件为例对整个喷涂过程进行计算机仿真，得出优化仿真结果。     

平面曲柄摇杆机构的计算机辅助动力学分析

对平面曲柄摇杆机构进行了运动学和动力学分析，并利用MATLAB6．5对其进行了计算机模拟分析，得出了平面曲柄摇杆机构各铰节点动态规律图，对分析机构的稳定性和优化设计具有一定的参考价值。     

变螺距诱导轮流场的数值模拟

在泵叶轮进口前置变螺距诱导轮能有效地提高泵机组的抗汽蚀性能。基于CFD技术,采用Fluent软件对变螺距诱导轮进行几何建模、选定边界条件等,数值模拟计算其内部流场。结合变螺距诱导轮的实例,给出了变螺距诱导轮内部速度与压力的分布情况以及粒子流动迹线图,得到了其内部流场的主要特征。     

装载机散热器模块进出口位置匹配试验

为提高车辆冷却系统中多散热器模块匹配的性能，对某装载机散热器模块在风洞测试平台上进行试验研究。试验散热器模块第1排是中冷器和液压油冷却器，第2排为水箱，第3排是变矩器油冷器。在风洞上对模块原型和在原型基础上互换变矩器油冷器进出油口位置的变型进行对比试验。试验结果表明，调整进出油口位置，能提高总的散热量，且对模块中单个散热器的性能影响更大。在设计中可以合理布置散热器进出油口位置，使得模块性能更加优异。     

新疆桃实生优选单株果实品质比较

以7个不同新疆桃种质资源为试材,比较其实生优选单株的果实品质,明确新疆桃实生优选单株的果实品质优劣,以期为新疆桃产业的发展提供新的种质资源.结果表明:7个新疆桃种质的单果质量范围在125.39～198.89 g,种质间差异较大,'毛桃四号'毛桃一号'毛桃三号'单果质量显著高于其它种质;7个新疆桃种质的可溶性固形物含量在9.88％～13.95％,除了'油桃二号'其它种质的可溶性固形物含量均超过了12％;7个新疆桃种质的可溶性糖含量范围在5.43％～13.91％,种质间差异较大,'毛桃四号'的可溶性糖含量显著高于其它种质;可滴定酸含量范围在0.57％～1.74％,种质间差异较大,'毛桃五号'油桃一号'的可滴定酸含量显著高于其它种质,7个新疆桃种质呈现出多样化的果实形状、颜色、风味.结合外在品质和内在品质综合分析,'毛桃四号'的果实品质综合性状表现最好,可以在新疆地区推广运用.