Metastatic immune infiltrates correlate with those of the primary tumour in canine osteosarcoma

Our lack of understanding of the immune microenvironment in canine osteosarcoma (cOSA) has limited the identification of potential immunotherapeutic targets. In particular, our ability to utilize readily available tissue from a dog's primary tumour to predict the type and extent of immune response in their pulmonary metastatic lesions is unknown. We, therefore, collected 21 matched pairs of primary tumours and pulmonary metastatic lesions from dogs with OSA and performed immunohistochemistry to quantify T‐lymphocyte (CD3), FOXP3+ cell, B‐lymphocyte (Pax‐5), and CD204+ macrophage infiltration. We found that T‐lymphocytes and FOXP3+ infiltrates in primary tumours positively correlated with that of metastatic lesions (ρ = 0.512, P = 0.038 and ρ = 0.698, P = 0.007, respectively), while a strong trend existed for CD204+ infiltrates (ρ = 0.404, P = 0.087). We also observed T‐ and B‐lymphocytes, and CD204+ macrophages to be significantly higher in a dog's pulmonary metastasis compared to their primary tumour (P = 0.018, P = 0.018, P = 0.016, respectively), while FOXP3+ cells were only significantly higher in metastases when all primary tumour and metastasis lesions were compared without pairing (P = 0.036). Together, these findings suggest that the metastatic immune microenvironment may be influenced by that of the primary cOSA, and that primary tumour immune biomarkers could potentially be applied to predict immunotherapeutic responses in gross metastatic disease. We, therefore, provide a rationale for the treatment of cOSA pulmonary metastases with immunotherapeutics that enhance the anti‐tumour activity of these immune cells, particularly in dogs with moderate to high immune cell infiltration in their primary tumours.     

结核分枝杆菌蛋白酶体结构与功能研究进展

结核病是由结核分枝杆菌(MTB)引起的一种慢性传染病,其发病机制比较复杂。本文介绍了MTB蛋白酶体结构及其激活因子的研究进展,阐述了MTB蛋白酶体生理功能,揭示了蛋白酶体在结核分枝杆菌的致病性、持留性、转录调控中发挥的重要作用。对结核分枝杆菌蛋白酶体结构和生理功能的深入研究,有助于进一步探索结核分枝杆菌持留致病新机制,也可为疫苗和抗结核新药的研发提供理论依据。     

对分割肉检疫监管的探讨

公众对肉类产品的需求逐渐由白条肉向品牌化、精细化的分割肉方向发展,相关产品的检验、检疫、监管已成为社会关注的热点。本文对三种不同类型肉品分割行为进行了分析,探讨检疫监管存在的问题,并提出明确监管主体、修订相关法律法规、建立追溯平台、强化主体责任的建议,供同仁参考。     

实验性鸡马立克氏病羽髓病变研究

通过光镜1电镜等方法对鸡马立克氏病羽髓组织进行了形态学研究。结果显示，羽髓远端坏死，羽髓中有多量肿瘤细胞浸润，肿瘤细胞呈弥漫性或局部性浸润，电镜观察，肿瘤细胞核异型性明显，核膜凹陷，核内出现假核内包含物，在一些羽髓中，巨噬细胞吞噬肿瘤细胞较多见，而且观察到一些肿瘤细胞发生调亡，结果表明，鸡马立克氏病羽髓病变十分明显，羽髓的形态学变化可用于鸡马立克氏病的诊断。     

关于建立畜禽生产信息化监督管理体系的设想

畜禽的饲养生产是动物源性食品的源头所在。保证畜禽安全生产、确保畜禽产品质量安全是每个畜牧业工作者的重要责任。本文提出了建立畜禽生产信息化监督管理体系的设想,通过建立畜禽养殖电子档案,设置兽药、饲料和饲料添加剂、兽用生物制品产品包装编码,搭建开放式畜禽生产信息查询监督平台等方式,让畜禽生产过程公开化、透明化,便于兽医管理部门开展监督执法工作和接受群众的查询与监督。     

鸵鸟肉深加工技术

1工艺流程原料选择→解冻→剔骨→切块→预煮→煎炒拌料→包装→真空封口→杀菌→贮运。2操作要点2.1解冻解冻的方法和条件:夏季解冻室温为16～20℃,冬季为10～15℃,解冻时间夏季12～24小时,冬季     

Glutathione content of in vivo and in vitro matured canine oocytes collected from different reproductive stages

Glutathione (GSH) concentrations of oocytes are considered as an important marker of the cytoplasmic maturation. The present study was designed to compare GSH concentrations of in vivo and in vitro matured canine oocytes. In vivo matured oocytes were collected 72 hr after ovulation by flushing fallopian tubes after laparotomy. Ovaries were collected from bitches with different reproductive stages, and collected oocytes were divided into 2 groups according to the size viz. < 120 microm and > 120 microm in diameter and cultured for 72 hr in Tissue Culture Medium-199 supplemented with 10% FBS, 2.2 mg/ml sodium bicarbonate, 2.0 microg/ml estrogen, 0.5 microg/ml FSH, 0.03 IU/ml hCG, and 1% penicillin-streptomycin solution in the presence or absence of 50 microM beta-mercaptoethanol. GSH concentrations were determined by the dithionitrobenzoic acid-glutathione disulfide (DTNB-GSSG) reductase recycling assay. GSH concentrations of immature canine oocytes were 2.9 and 3.8, 3.5 and 6.8, and 3.1 and 6.5 pM/oocyte for < 120 microm and > 120 microm in diameter oocyte groups at anestrous, follicular and luteal stage, respectively (P<0.05). In vivo matured oocytes had significantly higher GSH concentrations compared with in vitro matured oocytes. The GSH content was 19.2 pM/oocyte for in vivo matured oocytes, while 4.1 to 8.1 and 5.7 to 13.2 pM/oocyte for in vitro matured oocytes cultured in the absence or presence of beta-mercaptoethanol, respectively (P<0.05). Presence of beta-mercaptoethanol increased GSH synthesis in canine oocytes cultured in vitro, and oocytes collected from follicular and luteal stage was superior to anestrus oocytes.     

慈溪市土蜂濒临绝迹的生态因素

慈溪地处东海之滨、杭州湾南岸,总面积1154平方公里,慈溪属北亚热带季风气候区,气候温和湿润,四季分明。全境地势南高北低,呈丘陵、平原、滩涂三级台阶状朝杭州湾展开。     

赴美采购流产大豆价格变数加大

2009年5月27日,商务部组团赴美采购2000万吨大豆的“超级订单”,最终流产。双方没有像往年一样签订协议,一个重要原因是中方的报价没有得到美方的认同。而幕后的原因是,美方忌惮我国购买大豆用于收储,调控市场价格。     

鸡痘病毒282E4株2.2kbTK基因序列分析

将鸡痘病毒２８２Ｅ４弱毒株基因组中的３．７ｋｂＨｉｎｄⅢ片段克隆到ＫＳ（－）质粒中，亚克隆后，获得含ＴＫ基因正反方向插入的２．２ｋｂＨｉｎｄⅢ－ＣｌａＩ片段的质粒ｐＫＦＡ和ｐＫＦＢ，进而构建出正反方向的系列嵌套缺失质粒。用Ｔ７、Ｔ３引物所做的核苷酸序列分析表明，２．２ｋｂＨｉｎｄⅢ－ＣｌａＩ ＴＫ基因序列长为２１５９ｂｐ，含有两个完整的读码框架（ＯＲＥ）Ｘ和ＴＫ，并确证了ＴＫ基因内存在单一酶切点Ｎ