黄瓜全雌性基因连锁的AFLP和SCAR分子标记

 本研究以全雌品种‘戴多星’自交系和弱雌品种‘北京截头’自交系为双亲杂交获得F₁ ,然后得到F₂ 性型分离群体, 利用分离群体分组分析法(Bulked Segregant Analysis, BSA) 构建全雌和弱雌两个基因池, 筛选了64对AFLP选择性引物EcoR I-NN +Mse I-NNN组合, 发现EcoR I-TG +Mse I-CAC引物组合在全雌基因池中扩增出一条分子量为234 bp的特异带。经F₂ 代单株验证, 该特异条带能在全雌单株中稳定出现。以MAP MAKER (Version 310) 软件分析, 该标记与全雌性位点的连锁距离在617 cM。命名该连锁标记为TG/CAC₂₃₄。将该特异条带回收、克隆、测序, 设计特异SCAR引物, 再对F₂ 代单株基因组DNA进行扩增, 仅在全雌单株中扩增出1条分子量为166 bp 的特异带, 表明已成功地将与黄瓜全雌性连锁的AFLP标记转化为操作简便、表现稳定的SCAR标记, 该标记命名为SA₁₆₆。     

蝴蝶兰组织培养中的褐化控制研究

 系统研究了吸附剂(活性炭、PVP) 与柠檬酸、半胱氨酸、谷胱甘肽、维生素C和硫代硫酸钠5种抗氧化剂对蝴蝶兰组培中褐化的影响。结果表明: 活性炭能有效控制褐化和促进生长, 但不利于分化; 谷胱甘肽控制褐化的效果虽不及活性炭, 但综合效果最佳。     

中国果树类病毒的发生及其研究进展(英文)

概述了在中国发生且己鉴定明确的5种果树类病毒,即苹果绣果类病毒(Apple scar skin viroid,ASSVd)、梨泡状溃疡类病毒(Pear blister canker viroid,PBCVd)、葡萄黄斑类病毒-1(Grapevine yellow speckle viroid-1,GYSVd-1)、柑橘裂皮类病毒(Citrus exocortis viroid,CEVd)和桃潜隐花叶类病毒(Peachlatent mosaic viroid,PLMVd)的研究进展,包括病害的首次发现、症状特征、发病规律、检测方法与防治对策以及这些类病毒的生物学与分子生物学特性。     

不同化学物质打破葡萄、桃休眠的研究

以保护地栽培的葡萄、桃为试材,对氰氨类、细胞分裂素、赤霉酸和KNO3打破休眠的效果进行了研究。结果表明,1.5%单氰氨、11%石灰氮可明显促使葡萄提前萌芽,提早着色9-10d;50mg/LGA3和100mg/L6-BA对打破休眠基本无效。TDZ与单氰氨混用提高萌芽率的效果最好。在2个葡萄品种上,均没有观察到单氰氨或石灰氮处理对果穗或果实品质性状的不良影响。在华光油桃上,没有观察到化学物质对提早开花或成熟有明显的作用。对葡萄和桃上应用化学物质打破休眠的有关问题进行了讨论。     

日光温室内外金太阳杏光合特性的比较研究

为了探明杏对设施栽培的生理反应,对日光温室内外金太阳杏光合特性进行了比较研究。结果表明,日光温室栽培条件下,金太阳杏单位重量叶片的叶绿素含量增加;光(CO2)饱和点和光(CO2)补偿点降低鸦露地晴天净光合速率Pn日变化呈明显的双峰曲线,温室内也呈双峰线,但双峰出现原因不同。并且研究了影响光合作用的主要生态因子及变化规律,发现净光合速率与胞间CO2浓度、蒸腾作用、气温、叶温、光合有效辐射等因子相关。     

Malignant catarrhal fever-like disease in sheep after intranasal inoculation with ovine herpesvirus-2.

A malignant catarrhal fever (MCF)-like disease was induced experimentally in 3 sheep after aerosol inoculation with ovine herpesvirus-2 (OvHV-2). Each of 3 OvHV-2-negative sheep was nebulized with 2 ml of nasal secretions containing approximately 3.07 X 10(9) OvHV-2 DNA copies from a sheep experiencing an intensive viral-shedding episode. Ovine herpesvirus-2 DNA became detectable by polymerase chain reaction in the peripheral blood leukocytes of all 3 sheep within 3 days, and all 3 seroconverted between 6 and 8 days postinfection (PI). The sheep developed clinical signs, with copious mucopurulent nasal discharge and fever around 14 days PI. One of the 3 clinically affected sheep was euthanized at 18 days PI. Major lesions at necropsy were multifocal linear erosions and ulcers in mucosa of the cheeks, tongue, pharynx, and proximal esophagus and mild disseminated pneumonia. Microscopically, there was extensive moderate superficial histiocytic-lymphocytic rhinitis with epithelial dissociation and degeneration. Moderate multifocal histiocytic bronchointerstitial pneumonia was associated with loss of terminal bronchiolar epithelium. Lymphocytic vasculitis was present only in the lung. The remaining 2 sheep recovered clinically, approximately 25 days PI. The study revealed that clinical signs and lesions resembling MCF can develop when uninfected sheep are exposed to a high dose of aerosolized OvHV-2.     

番鸭在南京地区的适应性观察

番鸭在南京地区进行冬、夏两批饲养,均有较强的适应性。试验结果表明:冬、夏两 批饲养的番鸭只平均重量差异不显著(P>0．05)。夏季与冬季的料重比分别为1:2．62和1: 3．07,冬季饲养的鸭子饲料利用率低,料重比大于夏季。冬季在塑料大棚中饲养番鸭精饲料减 少1／3,添加青粗饲料能达到同样的增重效果。温度与增重无相关关系,相对湿度与番鸭的增 重呈负相关(P<0．01)。鸭舍内平均温度在11．05℃-30．93℃之间,相对湿度在76．28％以 下,番鸭能正常生长发育。环境温度在9．8℃以下,番鸭的生长发育受阻,饲料利用率降低。相 对湿度在77％以上,其增重受到影响。特别在低温高湿的环境中,严重地影响番鸭的生长。在 相同的饲养期内,周平均温度9．8℃,平均相对湿度91％,番鸭周平均增重22 g,周平均温度 27．42℃,平均相对湿度71．29％,番鸭周平均增重355 g,相差333 g(P<0．01)。     

樟子松在西北干旱沙区的光合日变化特征

在甘肃省民勤治沙站 ,用 Li- 62 0 0便携式光合仪分别测定人工栽植的 4龄、9龄和 2 0龄樟子松的净光合速率日进程 ,其结果表明 :在干旱沙区 ,9龄以上樟子松的光合速率在午前随光强和气温而上升 ,午后下降。其 4龄、9龄和 2 0龄樟子松的最大净光合速率分别为 8.7μmol· m- 2 · s- 1、62 .3μmol· m- 2 · s- 1、1 9.7μmol· m- 2 · s- 1。     

Association of polymorphism of Fc receptor γ chain gene at position-29 in promoter with the susceptibility to systemic lupus erythematosus in southern Chinese

AIM:To detect the association between the polymorphism of Fc receptor γ chain gene at position-29 in promoter and systemic lupus erythematosus(SLE).METHODS:The genotypes at position -29 in promoter of Fc receptor γ chain gene were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method in 180 patients with SLE and 140 ethnically matched controls in southern China.RESULTS:The frequencies of TT genotype(33.3%) and T allele (54.4%) at position -29 in patients with SLE were significantly higher than those in controls (17.2% and 42.9%, respectively), whereas, the frequencies of GG genotype (24.4%) and G allele (45.6%) in patients with SLE were remarkably lower than those in controls (31.4% and 57.1%, respectively) (P＜0.05). The TT genotype and T allele at position -29 were not associated with lupus nephritis in SLE patients (P＞0.05).CONCLUSION:Our results indicate that the T allele at position -29 in promoter of Fc receptor gene probably contributes to the susceptibility to SLE, but does not play a role in the occurrence of lupus nephritis.     

Effects of the juice of Fructus Hippophae (JFH) on thrombocytopenia induced by cyclophosphamide in rats

AIM:To investigate the effects of the juice of fructus hippophae(JFH) on thrombocytopenia in rats.METHODS:Forty-eight wistar rats were divided into control group, model group, Yixuesheng-Jiaonang (YSJN) group and JFH group. Rats were injected intraperitoneally with cyclophosphamide (CTX, 30 mg/kg) or saline once a day for 3 days. And then, Saline, YSJN or JFH was administered (ig) once a day for 11 days. On the second, fouth, sixth and eighth day, the thrombocyte were counted, the mean platelet volume (MPV) and PDW were examined. On the eighth day, the cAMP, cGMP in platelets and platelet aggregation function were also examined.RESULTS:JFH could shorten the time of blood coagulation, increase the count of platelet, and enhance the platelet aggregation function. The experiment also showed that the JFH could decrease cGMP content in platelet.CONCLUSION:JFH could enhance blood coagulation and quality of platelet and prevent thrombocytopenia induced by cyclophosphamide in rats.