茶氨酸合成酶基因的SNP挖掘和遗传定位

茶氨酸合成酶(Theanine synthetase,TS)基因是茶树茶氨酸代谢过程中的关键酶基因。本研究以氨基酸含量差异明显的亲本及其杂交所得F_1子代为研究材料,克隆TS基因的c DNA序列,挖掘其SNPs位点,并成功将杂合SNP位点定位在遗传连锁群上。研究结果显示,通过序列比对在亲本间检测到3个SNPs,验证得到1个杂合的位点SNP735。将此位点成功转化为dCAPS标记,该标记在子代中的基因型分离比为1∶1,利用该群体已构建的茶树遗传图谱进行遗传定位,将dCAPS标记定位在连锁群LG03上,相邻标记为TM299和TM517。联合此标记及其相邻标记与游离氨基酸总含量和茶氨酸含量进行统计分析,表明具有显著的相关性。     

播期对吉林省不同品种玉米生长发育及产量的影响

为探讨不同播期对吉林省春玉米生长发育和产量形成的影响,本试验以吉林省主栽品种通单258、华科425、农华106为材料,进行5个播期处理试验,播期设置分别为:4月28日(T1)、5月4日(T2)、5月11日(T3)、5月18日(T4)、5月25日(T5)。结果表明,随着播期推迟,各品种玉米生育期均不同程度缩短,并且播期越晚,干物质积累量越低。玉米开花期前,不同处理叶面积指数差异不显著,花后晚播处理叶面积指数下降快于早播处理。推迟播期,不同品种百粒重降低,产量下降,T1处理产量与T4、T5处理差异达显著水平。适时早播,有利于玉米产量的进一步提高。     

优良早熟玉米自交系KL3及其衍生系利用

自交系KL3是以441×1034为基础材料经多代自交选育而成的优良自交系,是黑龙江省早熟玉米育种的重要自交系,因其抗病性好、抗逆性强、配合力高、农艺性状好、熟期适中而被广泛应用。自交系KL3直接利用选育8个玉米品种,间接利用选育13个玉米品种,社会效益显著。     

靖粳26号的特征特性及高产栽培技术

靖粳26号系由云南省曲靖市农业科学院选育的优质粳稻新品种,具有良好的丰产性和优异的抗逆性,是一个适应性较广的优质、高产、耐寒、多抗粳稻新品种。     

气象因子对寒地水稻鞘腐病发生的影响

为明确主要气象因子对黑龙江省水稻主产区水稻鞘腐病发生的影响,从2007年开始连续10年进行定点调查,收集水稻鞘腐病发生时期的气象因子及病情指数,采用通径分析及逐步回归的方法,对影响水稻鞘腐病病情指数的气象因子进行了研究。结果表明,8月份的平均温度是对水稻鞘腐病影响最大的气象因子,其次为8月份的平均降雨量和9月份的平均日照时数。试验结果为适期施药防治水稻鞘腐病奠定了基础。     

扬州市稻田高效种养模式应用现状及发展对策

近年来,扬州市根据国家农业供给侧结构性调整要求,充分利用自然资源禀赋,开展了多种形式稻田种养模式的示范与推广。本文阐述了扬州市稻鸭共作、稻虾共作以及稻田改作荷藕-虾共作等高效种养模式的应用现状,以单纯种稻为对照,比较了各种模式的生产效益;分析了稻田高效种养模式示范推广过程中存在的主要问题,并提出了促进稻田高效种养模式可持续发展的若干对策。     

USP14 regulates H2O2 induced oxidative stress in H9c2 cells

AIM:To evaluate the effect of inhibiting ubiquitin-specific protease 14(USPl4) activity on oxidative stress induced by H₂O₂ of H9c2 cells.METHODS:The H9c2 cells were incubated with H₂O₂ at 25 μmol/L for 2 h to establish the oxidative stress injury model.The cells were divided into control group,H₂O₂ group,IU1 group (25 μmol/L or 50 μmol/L) and IU1+H₂O₂ group.The H9c2 cells activity was measured by MTS assay.The level of intracellular reactive oxygen species (ROS) and cell survival rate were analyzed by flow cytometry assay.The changes of the mitogen-activated protein kinase (MAPK) family related proteins were detected by Western blot.RESULTS:Compared with control group,the cell activity and the viability rate in H₂O₂ group were decreased (P<0.05),while the intracellular ROS,the protein levels of Bax/Bcl-2,P53,p-ERK1/2,p-JNK and p-P38 were increased (P<0.05).Compared with H₂O₂ group,the cell activity and the viability rate of the H9c2 cells in IU1+H₂O₂ group were increased (P<0.05),while the intracellular ROS,the protein levels of Bax/Bcl-2,P53,p-ERK1/2,p-JNK and p-P38 were decreased (P<0.05).CONCLUSION:Inhibition of USPl4 activity reduces the oxidative stress injury of the H9c2 cells.The mechanism may be related to inhibition of the MAPK signaling and down-regulation of apoptosis related proteins.     

绒毛白蜡新品种‘华雄’

 ‘华雄’是在白蜡属种质资源收集过程中发现并选育出的绒毛白蜡新品种。雄株,干形较直,当年生枝和纸质叶片上下表面均被密毛,耐盐碱性强。     

Protective effects of taurine on LPS-induced myocardial damage in rats

AIM: To investigate the effects of taurine on lipopolysaccharide (LPS)-induced myocardial damage in rats. METHODS: Healthy male SD rats (n=30) were randomly divided into control group (CON), LPS model group (LPS) and taurine treatment group (TAU). The rats in CON group and LPS group were intravenously injected with normal saline, and the rats in TAU group were injected with taurine (100 mg/kg). After 2 h, the rats in LPS group and TAU group were intraperitoneally injected with LPS at 10 mg/kg, and the rats in CON group were injected with normal saline. Six hours after injection of LPS, the blood samples were collected for determination of superoxide dismutase (SOD) activity, malondialdehyde (MDA) content, and tumor necrosis factor α (TNF-α) and interleukin-6 (IL-6) levels. The myocardial tissues were processed for histological examination and the analysis of Western blot. RESULTS: Compared with CON group, LPS significantly reduced SOD activity in the serum and heme oxygenase 1 (HO-1) protein expression in the myocardial tissues, increased the serum content of MDA and levels of TNF-α and IL-6. LPS also significantly elevated the levels of TNF-α and IL-6, and up-regulated the cyclooxygenase-2 (COX-2) expression and phosphorylation of nuclear factor kappa B (NF-κB) in the myocardial tissues. Taurine pretreatment significantly elevated SOD activity and HO-1 protein expression level, decreased the levels of COX-2, TNF-α, IL-6 and phosphorylated NF-κB. Histological observation showed that taurine reduced inflammatory response in the myocardial tissue. CONCLUSION: Taurine attenuates LPS-induced myocardial damage in rats. The beneficial effects of taurine may be associated with its reduction of p-NF-κB/COX-2 signaling by activation of HO-1/CO.     

不同栽培基质对霍山米斛总多糖含量的影响

以霍山米斛为试材,于采摘期采摘不同栽培基质培养的霍山米斛,采用苯酚-硫酸显色法测定总多糖含量,探究不同栽培基质对霍山米斛总多糖含量的影响。结果表明:不同栽培基质霍山米斛总多糖含量为S1(松树锯末+石子,低矮大棚)S3(松树皮+石子,低矮大棚)S4(椰子壳+石子,低矮大棚)S2(石子,低矮大棚)S5(石子,仿野生)。统计学分析表明,不同基质栽培的霍山米斛总多糖含量的差异存在统计学意义,聚类分析数据显示,5种栽培基质的霍山米斛可以分为4类:S1单独一类、S3单独一类、S4单独一类、S2和S5一类。试验旨为霍山米斛的最佳栽培基质选择和初步判定栽培基质的种类提供依据,以期充分地开发霍山米斛资源。